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| 1 | Therapeutic imaging window of cerebral infarction revealed by multisequence magnetic resonance imaging An animal and clinical study显示文摘In this study, we established a Wistar rat model of right middle cerebral artery occlusion and observed pathological imaging changes (T2-weighted imaging [T2WI], T2FLAIR, and diffusion-weighted imaging [DWI]) following cerebral infarction. The pathological changes were divided into three phases: early cerebral infarction, middle cerebral infarction, and late cerebral infarction. In the early cerebral infarction phase (less than 2 hours post-infarction), there was evidence of intracellular edema, which improved after reperfusion. This improvement was defined as the ischemic penumbra. In this phase, a high DWI signal and a low apparent diffusion coefficient were observed in the right basal ganglia region. By contrast, there were no abnormal T2WI and T2FLAIR signals. For the middle cerebral infarction phase (2-4 hours post-infarction), a mixed edema was observed. After reperfusion, there was a mild improvement in cell edema, while the angioedema became more serious. A high DWI signal and a low apparent diffusion coefficient signal were observed, and some rats showed high T2WI and T2FLAIR signals. For the late cerebral infarction phase (4-6 hours post-infarction), significant angioedema was visible in the infarction site. After reperfusion, there was a significant increase in angioedema, while there was evidence of hemorrhage and necrosis. A mixed signal was observed on DWI, while a high apparent diffusion coefficient signal, a high T2WI signal, and a high T2FLAIR signal were also observed. All 86 cerebral infarction patients were subjected to T2WI, T2FLAIR, and DWI. MRI results of clinic data similar to the early infarction phase of animal experiments were found in 51 patients, for which 10 patients (10/51) had an onset time greater than 6 hours. A total of 35 patients had MRI results similar to the middle and late infarction phase of animal experiments, of which eight patients (8/35) had an onset time less than 6 hours. These data suggest that defining the 'therapeutic time window' as the time 6 hours after infarction may not be suitable for all patients. Integrated application of MRI sequences including T2WI, T2FLAIR, DW-MRI, and apparent diffusion coefficient mapping should be used to examine the ischemic penumbra, which may provide valuable information for identifying the 'therapeutic time window'. | Hong Lu Hui Hu Zhanping He Xiangjun Han Jing Chen Rong Tu | 2012 | Neural Regeneration Research2012，7，31： | 16 |
| 2 | Gene interference regulates aquaporin-4 expression in swollen tissue of rats with cerebral ischemic edema Correlation with variation in apparent diffusion coefficient显示文摘To investigate the effects of mRNA interference on aquaporin-4 expression in swollen tissue of rats with ischemic cerebral edema, and diagnose the significance of diffusion-weighted MRI, we injected 5 μL shRNA- aquaporin-4 (control group) or siRNA- aquaporin-4 solution (1:800) (RNA interference group) into the rat right basal ganglia immediately before occlusion of the middle cerebral artery. At 0.25 hours after occlusion of the middle cerebral artery, diffusion-weighted MRI displayed a high signal; within 2 hours, the relative apparent diffusion coefficient decreased markedly, aquaporin-4 expression increased rapidly, and intracellular edema was obviously aggravated; at 4 and 6 hours, the relative apparent diffusion coefficient slowly returned to control levels, aquaporin-4 expression slightly increased, and angioedema was observed. In the RNA interference group, during 0.25- 6 hours after injection of siRNA- aquaporin-4 solution, the relative apparent diffusion coefficient slightly fluctuated and aquaporin-4 expression was upregulated; during 0.5-4 hours, the relative apparent diffusion coefficient was significantly higher, while aquaporin-4 expression was significantly lower when compared with the control group, and intracellular edema was markedly reduced; at 0.25 and 6 hours, the relative apparent diffusion coefficient and aquaporin-4 expression were similar when compared with the control group; obvious angioedema remained at 6 hours. Pearson's correlation test results showed that aquaporin-4 expression was negatively correlated with the apparent diffusion coefficient (r = -0.806, P < 0.01). These findings suggest that upregulated aquaporin-4 expression is likely to be the main molecular mechanism of intracellular edema and may be the molecular basis for decreased relative apparent diffusion coefficient. Aquaporin-4 gene interference can effectively inhibit the upregulation of aquaporin-4 expression during the stage of intracellular edema with time-effectiveness. Moreover, diffusion-weighted MRI can accurately detect intracellular edema. | Hui Hu Hong Lu Zhanping He Xiangjun Han Jing Chen Rong Tu | 2012 | Neural Regeneration Research2012，7，21： | 14 |
| 3 | Sediment delivery ratio and its uncertainties on flood event scale:Quantification for the Lower Yellow River显示文摘Sediment delivery ratio(SDR)for fluvial rivers was formulated with sediment rating curve.The observed data of SDR on flood event scale of the Lower Yellow River(LYR)were adopted to examine the formulation and to calibrate the model parameters.A regression formula of SDR was then established and its 95%prediction interval was accordingly quantified to represent its overall uncertainties.Three types of factors including diversity of the incoming flow conditions,river self-regulation processes,and human activities were ascribed to the uncertainties.The following were shown:(1)With the incoming sediment coefficient(ISC)being a variable,it was not necessary to adopt the incoming flow discharge as the second variable in the formulation of SDR;and(2)ISC=0.003 and therefore SDR=2 might be a threshold for distinguishing the characteristics of sediment transport within the LYR.These findings would highlight sediment transport characteristics on the scale of flood event and contribute to uncertainty based analysis of water volume required for sediment transport and channel maintenance of the LYR. | FU XuDong 1,JIANG LiWei 1,WU BaoSheng 1,HU ChunHong 2,WANG GuangQian 1 &FEI XiangJun 1 1State Key Laboratory of Hydroscience and Engineering,Tsinghua University,Beijing 100084,China 2China Institute of Hydropower and Water Resources Research,Beijing 100044,China | 2010 | Science China(Technological Sciences)2010，53，3： | 7 |
| 4 | MRS and diffusion tensor image in mild traumatic brain injuries显示文摘Objective:To analyze characters of magnetic resonance spectroscopy(MRS) and diffusion tensor imaging(DTI) in the diagnosis of mild trauma brain injuries(MTBI) in frontal lobe and to compare with conventional magnetic resonance imaging(MRI).Methods:A total of 21 patients were selected,who all aged 12-51 years old and had injury within 24 hours.Computer tomography (CT) and the Glasgow Coma Scale were used to evaluate the degree of injury.All patients were diagnosed as MTBI,and 19 had conventional MRI,MRS and DTI.The major parameters of MRS were Probe-P sequence,TE= 144 or 35 ms,and both single voxel spectrum and chemical shift imging were included.The major parameters of DTI were diffusion directions =15,b value = 1000 s/mm^2. Frational anisotropic(FA) map and average ADC map were obtained to evaluate DTI result. Positive deletion ratio was observed and the imaging changes were compared between injured side and normal side.Results:All 21 patients had CT scan and Glasgow scale.A total of 19 patients had conventional MRI.DTI and MRS.Results of CT and conventional MRI showed no significant abnormality in lobe,and Glasgow scale showed mild type.MRS result showed significant decrease in N-acetyl aspartate(NAA) and NAA/creatine(Cr) in 13 cases(68.4%) (P<0.001),and increase in lactic acid(Lac) in 7 cases(36.8%).FA mapping of the frontal lobe displayed significant changes in 7 cases(36.8%),with 5 out of the 7 cases having increase in FA value.And there was no significant difference in average ADC.Conclusions:MRS and DTI might be more sensible than other methods,such as CT and conventional MRI in diagnosis of MTBI.The particular changes were reduced NAA and increased Lac for MRS.and increased FA values for DTI. | Jing Chen Hu Jin Ying Zhang Qizhou Liang Haiyan Liao Ziyi Guo Xiangjun Han | 2012 | Asian Pacific Journal of Tropical Medicine2012，5，1： | 6 |
| 5 | Rapid isolation and immune profiling of SARS-CoV-2 specific memory B cell in convalescent COVID-19 patients via LIBRA-seq显示文摘B cell response plays a critical role against SARS-CoV-2 infection.However,little is known about the diversity and frequency of the paired SARS-CoV-2 antigen-specific BCR repertoire after SARS-CoV-2 infection.Here,we performed single-cell RNA sequencing and VDJ sequencing using the memory and plasma B cells isolated from five convalescent COVID-19 patients,and analyzed the spectrum and transcriptional heterogeneity of antibody immune responses.Via linking BCR to antigen specificity through sequencing(LIBRA-seq),we identified a distinct activated memory B cell subgroup(CD11c^(high) CD95^(high))had a higher proportion of SARS-CoV-2 antigen-labeled cells compared with memory B cells.Our results revealed the diversity of paired BCR repertoire and the non-stochastic pairing of SARS-CoV-2 antigen-specific immunoglobulin heavy and light chains after SARS-CoV-2 infection.The public antibody clonotypes were shared by distinct convalescent individuals.Moreover,several antibodies isolated by LIBRA-seq showed high binding affinity against SARS-CoV-2 receptor-binding domain(RBD)or nucleoprotein(NP)via ELISA assay.Two RBD-reactive antibodies C14646P3S and C2767P3S isolated by LIBRA-seq exhibited high neutralizing activities against both pseudotyped and authentic SARS-CoV-2 viruses in vitro.Our study provides fundamental insights into B cell response following SARS-CoV-2 infection at the single-cell level. | Bing He Shuning Liu Yuanyuan Wang Mengxin Xu Wei Cai Jia Liu Wendi Bai Shupei Ye Yong Ma Hengrui Hu Huicui Meng Tao Sun Yanling Li Huanle Luo Mang Shi Xiangjun Du Wenjing Zhao Shoudeng Chen Jingyi Yang Haipeng Zhu Yusheng Jie Yuedong Yang Deyin Guo Qiao Wang Yuwen Liu Huimin Yan Manli Wang Yao-Qing Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 2 |
| 6 | Cloning and expression analysis of the nuclear factor erythroid 2-related factor 2(Nrf2)gene of grass carp(Ctenopharyngodon idellus)and the dietary effect of Eucommia ulmoides on gene expression显示文摘Nuclear factor erythroid 2-related factor 2(Nrf2)is an important transcription factor in the regulation of redox homeostasis,which plays a vital role in metabolism,the cell cycle,and cell protection.In this study,the complete cDNA sequence of Nrf2 gene of grass carp was obtained by rapid amplification of cDNA ends-polymerase chain reaction(RACE-PCR),and the characteristics of Nrf2 proteins predicted from gene sequencing were analyzed by bioinformatics methods.Tissue differential expression patterns were also detected by Real-time Fluorescence quantitative PCR(qRT-PCR).Then,Eucommia ulmoides(EU),a traditional Chinese medicine,was added into diet with an inclusion of 2%to feed grass carp for 3 months to investigate the effects on the expression of Nrf2 gene in tissues.The results showed that the full-length of Nrf2 gene of grass carp is 2760 bp,encoding 593 amino acids,and the predicted Nrf2 protein is a hydrophilic and non-secretory protein.Grass carp Nrf2 gene showed higher expression in kidney,gill,liver,spleen,and blood tissues than in other tissues.In the feeding trial,dietary EU significantly promoted the Nrf2 mRNA expression in kidney,gill,and liver tissues after three months feeding.The results suggest that Nrf2 plays an important role in the anti-oxidative capacity of grass carp and its expression is enhanced by dietary EU. | Junpeng Wang Jun Xie Zhihan Tu Jing Wei Wenqian Pan Jing Hu Yi Zhang Xiangjun Leng Xiaoqin Li | 2018 | Aquaculture and Fisheries2018，3，5： | 2 |
| 7 | Polymorphisms in thymidylate synthase gene and susceptibility to breast cancer in a Chinese population:a case-control analysis显示文摘 | Xiangjun Zhai Jun Gao Zhibin Hu | 2006 | BMC Cancer2006，25，6： | 1 |
| 8 | Potentially functional genetic variants in microRNA processing genes and risk of HBV‐related hepatocellular carcinoma显示文摘 | Li Liu Jiaze An Jibin Liu Juan Wen Xiangjun Zhai Yao Liu Shandong Pan Jie Jiang Yang Wen Zheng Liu Yixin Zhang Jianguo Chen Jinliang Xing Guozhong Ji Hongbing Shen Zhibin Hu Zhining Fan | 2013 | Mol Carcinog2013，，1： | 1 |
| 9 | Dendritic - cell - based technology landscape : Insights from patents and cita- tion networks 显示文摘 | KONG XIANGJUN HU YUANJIA CAI ZHIFANG | 2015 | Human Vaccines & Immunotherapeutics2015，11，3： | 1 |
| 10 | Application of HNMR-based inetabolomics for detecting injury induced by long-term microwave exposure in Wistar rat' urine 显示文摘 | Wang Lifeng Hu Xiangjun Peng Ruiyun | 2012 | Anal Bioanal Chem2012，404，1： | 1 |
| 11 | Circulating exosome-like vesicles of humans with nondiabetic obesity impaired isletβ-cell proliferation,which was associated with decreased Omentin-1 protein cargo显示文摘The regulation ofβ-cell mass in the status of nondiabetic obesity remains not well understood.We aimed to investigate the role of circulating exosome-like vesicles(ELVs)isolated from humans with simple obesity in the regulation of isletβ-cell mass.Between June 2017 and July 2019,81 subjects with simple obesity and 102 healthy volunteers with normal weight were recruited.ELVs were isolated by ultra-centrifugation.The proliferations ofβ-cells and islets were measured by 5-ethynl-2′-deoxyuridine(EdU).Protein components in ELVs were identified by Quantitative Proteomic Analysis and verified by Western blot and ELISA.The role of specific exosomal protein was analyzed by gain-of-function approach in ELVs released by 3T3-L1 preadipocytes.Circulating ELVs from subjects with simple obesity inhibitedβ-cell proliferation in vitro without affecting its apoptosis,secretion,and inflammation.The protein levels of Rictor and Omentin-1 were downregulated in circulating ELVs from subjects with simple obesity and associated with the obesity-linked pathologic conditions.The ELV-carried Omentin-1 and Omentin-1 protein per se were validated to increaseβ-cell proliferation and activate Akt signaling pathway.Moreover,Omentin-1 in ELVs was downregulated by insulin.The circulating ELVs may act as a negative regulator forβ-cell mass in nondiabetic obesity through inhibitingβ-cell proliferation.This effect was associated with downregulated Omentin-1 protein in ELVs.This newly identified ELV-carried protein could be a mediator linking insulin resistance to impairedβ-cell proliferation and a new potential target for increasingβ-cell mass in obesity and T2DM. | Qian Ge Xinxin Xie Xiangjun Chen Rongfeng Huang Cheng-Xue Rui Qianna Zhen Renzhi Hu Min Wu Xiaoqiu Xiao Xi Li | 2022 | Genes & Diseases2022，9，4： | 1 |
| 12 | Fabric-substrated capacitive biopotential sensors enhanced by dielectric nanoparticles显示文摘Wearable biopotential sensing devices are essential to long-term and real-time monitoring of human health.Non-contact,capacitive sensing electrodes prevent potential skin iritations,and are thus beneficial for long-term monitoring.Existing capacitive electrodes are either connected to a separate control circuit via external wires or have limited sensing capacitances,which leads to low signal qualities.This study demonstrates a stretchable capacitive sensing device with integrated electrodes and control electronics,with enhanced signal qualities.The electrodes and the control electronics are fabricated on a common fabric substrate for breathability and strain-limiting protection.The stretchable electrodes are based on an island-bridge design with a stretchability as high as-100%,and an area ratio as high as-80%.By using a dielectric calcium copper titanate(CCTO)composite as the adhesive layer,the electrode capacitance can be increased,yielding an enhanced signal-to-noise ratio(SNR)in the acquired biopotentials.This device offers a convenient and comfortable approach for long-term non-contact monitoring of biopotential signals. | Xiangjun Chen Xiaoxiang Gao Akihiro Nomoto Keren Shi Muyang Lin Hongjie Hu Yue Gu Yangzhi Zhu Zhuohong Wu Xue Chen Xinyu Wang Baiyan Qi Sai Zhou Hong Ding Sheng Xu | 2021 | Nano Research2021，14，9： | 1 |
| 13 | A low-energy ion spectrometer with half-space entrance for three-axis stabilized spacecraft显示文摘A low-energy ion spectrometer(LEIS) for use aboard three-axis stabilized spacecraft has been developed to measure ion energy per charge distribution in three-dimensional space with good energy-, angular-and temporal-resolutions. For the standard top-hat electrostatic analyzer used widely in space plasma detection, three-axis stabilized spacecraft makes it difficult to obtain complete coverage of all possible ion arrival directions. We have designed angular scanning deflectors supplementing to a cylindrically symmetric top-hat electrostatic analyzer to provide a half-space field of view as 360°×90°(–45°–+45°), and fabricated the LEIS flight model for detecting magnetospheric ions in geosynchronous orbit. The performance of this payload has been evaluated in detail by a series of simulation and environmental tests, and the payload has also been calibrated through laboratory experiments using a low-energy ion source. The results show that capabilities of the LEIS payload are in accordance with the requirements of a magnetospheric mission. | HU RenXiang SHAN Xu YUAN GuangYuan WANG ShuWen ZHANG WeiHang QI Wei CAO Zhe LI YiRen CHEN ManMing YANG XiaoPing WANG Bo SHAO SiPei LI Feng ZHONG XiaoQing FAN Dan HAO XinJun FENG ChangQing SU ZhenPeng SHEN ChengLong LI Xin DAI GuYue QIU BingLin PAN ZongHao LIU Kai XU ChunKai LIU ShuBin AN Qi ZHANG TieLong WANG YuMing CHEN XiangJun | 2019 | Science China(Technological Sciences)2019，62，6： | 1 |
| 14 | Puerarin inhibits C -reactive protein expression via suppression of nuclear factor kappaB activation in lipopolysaccharide - induced peripheral blood mononuclear cells of patients with stable angina pectoris 显示文摘 | Yang Xiangjun Hu Wenzhi Zhang Qin | 2010 | Basic Clin Pharmacol Toxicol2010，107，2： | 1 |
| 15 | Identity-based broadcast signcryption 显示文摘 | Fagen Li Xiangjun Xin Yupu Hu | 2008 | Computer Standards & Interfaces2008，30，12： | 1 |
| 16 | Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish显示文摘 | An Xiao Zhanxiang Wang Yingying Hu Yingdan Wu Zhou Luo Zhipeng Yang Yao Zu Wenyuan Li Peng Huang Xiangjun Tong Zuoyan Zhu Shuo Lin Bo Zhang | 2013 | Nucleic Acids Research2013，，14： | 1 |
| 17 | A pairing-based signcryption scheme using self-certified public keys显示文摘 | Li Fagen Xin Xiangjun Hu Yupu | 2007 | International Journal of Computers and Applications2007，29，3： | 1 |
| 18 | Genetic variants in pseudogene E2F3P1 confer risk for HBV-related hepatocellular carcinoma in a Chinese population显示文摘Recent studies showed that pseudogenes can regulate the expression of their coding gene partners by competing for miRNAs. The E2F family plays a crucial role in the control of cell cycle checkpoint. E2F3P1 is a pseudogene of E2F3. Few studies focused on genetic variations on pseudogenes. In this study, we performed a case-control study to assess the association between single nucleotide polymorphisms (SNPs) in E2F3P1 and hepatocellular carcinoma (HCC) risk in 1050 hepatitis B virus (HBV)-positive HCC cases and 1050 chronic HBV carriers. Logistic regression analysis was applied to estimate odds ratios (ORs) and 95% confidence intervals (CIs) for the associations between genotypes and HCC risk. We found that the variant CT/TT genotypes of rs1838149 were associated with a significantly decreased risk of HCC (adjusted OR=0.66, 95% CIs=0.51-0.86, P=0.002) compared to those with wildtype CC homozygote. Furthermore, the AA genotype of rs9909601 had an increased HCC risk with an adjusted OR of 1.41 (95% CIs=1.07-1.86), and the A allele of rs9909601 was significantly associated with HCC risk compared to those with the G allele (adjusted OR=1.17, 95% CIs=1.03-1.33, P=0.017). These results indicate that genetic variations in the pseudogene E2F3P1 may confer HCC risk. | Li Liu Yao Liu Jibin Liu Xiangjun Zhai Juan Wen Kaipeng Xie Hongbing Shen Zhibin Hu Zhining Fan | 2013 | The Journal of Biomedical Research2013，27，3： | 1 |
| 19 | Effects of combined substitution of Dy and Mo or Si on the crystal structures and magnetic properties of Nd_(1-y)Dy_yFe_(11-x)TiM_x(M=Mo,Si) compounds显示文摘A series of Nd1-yDyyFe11-xTiMx(M=Mo,Si) alloys have been prepared by arc melting and studied by X-ray diffraction and neutron diffraction.All samples are found to crystallize in the ThMn12-type structure.The lattice parameters a and c and unit cell volume V of Nd0.5Dy0.5Fe11-xTiMox alloys increase linearly with increasing content of Mo(x),while the lattice parameters a and c and unit cell volume V of Nd0.5Dy0.5Fe11-xTiSix alloys decrease linearly with increasing content of Si(x).In NdyDy1-yFe11-xTiMx(M=Mo,Si) compounds,Ti and Mo atoms preferentially occupy the 8i sites and Si atoms preferentially occupy the 8j and 8f sites.Magnetic measurements show that the substitution of Fe by either Mo or Si leads to a decrease in the Curie temperature. | LI XiangJun,ZHENG Na,HU ZhongBo & YUAN ZhuoBin College of Chemistry and Chemical Engineering,Graduate University of Chinese Academy of Sciences,Beijing 100049,China | 2009 | Science China Chemistry2009，52，8： | 0 |
| 20 | TPA Enhances Growth Horm one (GH) Secretion Effect ofGH-Releasing Horm one (GHRH) by Hum an gsp-PositivePi-tuitary Som atotrophinom as显示文摘In recent years, one of the most exciting advances in the researches of pituitary adenomas is the discovery that 30 %-40 % of human pituitary somatotrophinomas carry somatic mutations of the gene for the α subunit of the stimulatory GTP binding protein, G s (G sα). These mutations, termed gsp oncogenes, may play an important role in the tumorigenesis of pituitary adenomas. Of 10 somatotrophinomas examined, 3 (30 %) were proved to be gsp positive, as determined by sequence analysis of DNA generated by the polymerase chain reaction (PCR). GHRH exerted a significant stimulatory effect on GH secretion in 2 of 3 gsp positive and 4 of 7 gsp negative tumors. Moreover, phorbol ester, 1, 2 tetradecanoylphorbol 13 acetate (TPA), enhanced stimulation of lated the GH secretion effect exerted by GHRH in gsp positive somatotrophinomas, whereas this effect was not observed in gsp negative tumors. This result suggests that the protein kinase C signal system as well as adenylyl cyclase cAMP protein kinase A intracellular signal transduction system plays a pivotal role in GH secretory control of GHRH, which may work together via a cross talk mechanism. | LEI Ting , BAI Xiangjun , HU Wenan , XUE Delin , JIANG Xianhui Department of Neurosurgery, Tongji Hospital, Tongji Medical University, Wuhan 430030 | 1999 | Journal of Huazhong University of Science and Technology(Medical Sciences)1999，19，3： | 0 |
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