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| 1 | Advances and Trends on Tube Bending Forming Technologies显示文摘作为有庞大的数量和差异的一个种关键部件,弄弯的试管部分从材料和结构方面为无足轻重的人和高张力产品满足增加的需要。弄弯的试管广泛地在象航空,太空,造船,汽车,精力和护理那样的许多高端工业被使用了。弯曲的试管成为了为小产品形成生产技术的钥匙之一。经由把特征和多重缺点弄弯的分析,在试管弯曲探索普通问题上的进展在穹隆之内面关于起皱的不稳定性被总结,墙变瘦(裂开) 在拱背, springback 现象,剖面图变丑,形成的限制和过程 / 工具设计 / 优化。一些当前发达的弯曲技术以他们的优点和限制被考察。最后,鉴于高效的建筑群的迫切要求,在航空与 difficult-to-deform 和小材料把试管部件弄弯,太空域,发展趋势和相应挑战为认识到把变丑弄弯的精确、高效率的试管被介绍。 | YANG He LI Heng ZHANG Zhiyong ZHAN Mei LIU Jing LI Guanjun | 2012 | Chinese Journal of Aeronautics2012，25，1： | 86 |
| 2 | Forming limits under multi-index constraints in NC bending of aluminum alloy thin-walled tubes with large diameters显示文摘With increasing diameters of aluminum alloy thin-walled tubes (AATTs), the tube forming limits, i.e. the minimum bending factors, and their predictions under multi-index constraints including wrinkling, thinning and flattening have been being a key problem to be urgently solved for improving tube forming potential in numerical control (NC) bending processes of AATTs with large diameters. Thus in this paper, a search algorithm of the forming limits is put forward based on a 3D elastic-plastic finite element (FE) model and a wrinkling energy prediction model for the bending processes under axial compression loading (ACL) or not. This algorithm enables to be considered the effects of process parameter combinations including die, friction parameters on the multi-indices. Based on this algorithm, the forming limits of the different size tubes are obtained, and the roles of the process parameter combinations in enabling the limit bending processes are also revealed. The followings are found: the first, within the appropriate ranges of friction and clearances between the different dies and the tubes enabling the bending processes with smaller bending factors, the ACL enables the tube limit bending processes after a decrease of the mandrel ball thickness and diameters; then, without considering the effects of the tube geometry sizes on the tube constitutive equations, the forming limits will be decided by the limit thinning values for the tubes with diameters smaller than 80 mm, while the wrinkling for the tubes with diameters no less than 80 mm. The forming limits obtained from this algorithm are smaller than the analytical results, and reduced by 57.39%; the last, the roles of the process parameter combinations in enabling the limit bending processes are verified by experimental results. | YAN Jing, YANG He, ZHAN Mei & LI Heng College of Materials Science and Engineering, State Key Laboratory of Solidification Processing, Northwestern Polytechnical University, Xi’an 710072, China | 2010 | Science China(Technological Sciences)2010，53，2： | 21 |
| 3 | Effects of four regulating-intestine prescriptions on pathology and ultrastructure of colon tissue in rats with ulcerative colitis显示文摘AIM: To observe different histomorphologic changes of ulcerative colitis (UC) rats that were treated with four regulating-intestine prescriptions (FRIP), to investigate the curative effects of FRIP and to analyze their treatment mechanism.METHODS: The UC rat model was made by the method of 2,4-dinitro chloro benzene (DNCB) immunity and acetic acid local enema. Ninety-eight SD rats were randomly divided into seven groups, namely, the normal control group, model group, salicylazosulfapyridine (SASP) group,Wumeiwan (WMW) group, Baitouwengtang (BTWT) group,Senglingbaishusan (SLBSS) group, and Tongxieyaofang (TXYF) group. Each group had 14 rats (with equal ratio of male and female). The six animal model groups of UC-SASP, TXYF, WMW, BTWT, SLBSS, TXYF-were treated by distilled water except the normal control group. Changes of the rat's general conditions after treatment were respectively observed, the colon tissue damage scores were given out, the pathology of colonic mucosa and changes of ultrastructure were analyzed.RESULTS: Different pathological changes on histology were shown after treatment by FRIP. The colon tissue damage score in model group was higher than that of FRIP groups and SASP group (q = 4.59, 4.77, P<0.05 or q = 5.48,6.25, 5.97, P<0.01). The scores of WMW group, BTWT group and SLBSS group were lower than that of SASP (q = 4.13, P<0.05 or q = 5.31, 5.12, P<0.01). There was no remarkable difference between the damage score of TXYF group and SASP group (q = 3.75, P>0.05). In addition, some apoptosis cells were found in the pathologic control group.CONCLUSION: The model made with DNCB and acetic acid was successful, and FRIP had better curative effect and WMW was the best curative effect, BTW, SLBSS and TXYF were similar to SASP, and we discovered that apoptosis was possibly related to UC. | Heng Fan Ming-Yi Qiu Jia-Jun Mei Guan-Xin Shen Song-Lin Liu Rui Chen | 2005 | World Journal of Gastroenterology2005，11，31： | 19 |
| 4 | Springback prediction of thick-walled high-strength titanium tube bending显示文摘Signifcant springback occurs after tube rotary-draw-bending(RDB),especially for a high-strength Ti–3Al–2.5V tube(HSTT) due to its high ratio of yield strength to Young's modulus.The combination scheme of explicit and implicit is preferred to predict the springback.This simulation strategy includes several numerical parameters,such as element type,number of elements through thickness(NEL),element size,etc.However,the influences of these parameters on springback prediction accuracy are not fully understood.Thus,taking the geometrical specifcation9.525 mm · 0.508 mm of a HSTT as the objective,the effects of numerical parameters on prediction accuracy and computation effciency of springback simulation of HSTT RDB are investigated.The simulated springback results are compared with experimental ones.The main results are:(1) solid and continuum-shell elements predict the experimental results well;(2) for C3D8R elements,NEL of at least 3 is required to obtain reliable results and a relative error of 29% can occur as NEL is varied in the range of 1–3;(3) specifying damping factor typically works well in Abaqus/Emplicit simulation of springback and the springback results are sensitive to the magnitude of damping factor.In addition,the explanations of the effect rules are given and a guideline is added. | Song Feifei Yang He Li Heng Zhan Mei Li Guangjun | 2013 | Chinese Journal of Aeronautics2013，26，5： | 15 |
| 5 | Expression of Ki-67, galectin-3, fragile histidine triad, and parafibromin in malignant and benign parathyroid tumors显示文摘 | WANG Ou WANG Chun-yan SHI Jie NIE Min XIA Wei-bo LI Mei JIANG Yan GUAN Heng MENG Xun-wu XING Xiao-ping | 2012 | Chinese Medical Journal2012，，16： | 8 |
| 6 | Cu(OAc)_2 catalyzed Sonogashira cross-coupling reaction in amines显示文摘简单 Cu (OAc ) 联合协议的 2 催化 Sonogashira 被介绍。有终端炔属羟的许多芳基卤的政变石楠顺利被进行负担得起相应需要的产品在,这被发现对优秀收益中等,用 Cu (OAc ) 2 作为催化剂和 Et3N 作为溶剂。 | Sheng Mei Guo Chen Liang Deng Jin Heng Li | 2007 | Chinese Chemical Letters2007，18，1： | 8 |
| 7 | The heterogeneity of islet autoantibodies and the progression of islet failure in type 1 diabetic patients显示文摘Type 1 diabetes mellitus is heterogeneous in many facets. The patients suffered from type 1 diabetes present several levels of islet function as well as variable number and type of islet-specific autoantibodies. This study was to investigate prevalence and heterogeneity of the islet autoantibodies and clinical phenotypes of type 1 diabetes mellitus; and also discussed the process of islet failure and its risk factors in Chinese type 1 diabetic patients. A total of 1,291 type 1 diabetic patients were enrolled in this study. Demographic information was collected. Laboratory tests including mixed-meal tolerance test, human leukocyte antigen alleles, hemoglobin A1 c, lipids, thyroid function and islet autoantibodies were conducted. The frequency of islet-specific autoantibody in newly diagnosed T1 DM patients(duration shorter than half year) was 73% in East China. According to binary logistic regressions, autoantibody positivity, longer duration and lower Body Mass Index were the risk factors of islet failure. As the disease developed, autoantibodies against glutamic acid decarboxylase declined as well as the other two autoantibodies against zinc transporter 8 and islet antigen 2. The decrease of autoantibodies was positively correlated with aggressive beta cell destruction. Autoantibodies can facilitate the identification of classic T1 DM from other subtypes and predict the progression of islet failure. As there were obvious heterogeneity in autoantibodies and clinical manifestation in different phenotypes of the disease, we should take more factors into consideration when identifying type 1 diabetes mellitus. | Jin Liu Lingling Bian Li Ji Yang Chen Heng Chen Yong Gu Bingqin Ma Wei Gu Xinyu Xu Yun Shi Jian Wang Dalong Zhu Zilin Sun Jianhua Ma Hui Jin Xing Shi Heng Miao Bing Xin Yan Zhu Zhenwen Zhang Ruifang Bu Lan Xu Guangde Shi Wei Tang Wei Li Dongmei Zhou Jun Liang Xingbo Cheng Bimin Shi Jixiang Dong Ji Hu Chen Fang Shao Zhong Weinan Yu Weiping Lu Chenguang Wu Li Qian Jiancheng Yu Jialin Gao Xiaoqiang Fei Qingqing Zhang Xueqin Wang Shiwei Cui Jinluo Cheng Ning Xu Guofeng Wang Guoqing Han Chunrong Xu Yun Xie Minmin An Wei Zhang Zhixiao Wang Yun Cai Qi Fu Yu Fu Shuai Zheng Fan Yang Qingfang Hu Hao Dai Yu Jin Zheng Zhang Kuanfeng Xu Yifan Li Jie Shen Hongwen Zhou Wei He Xuqin Zheng Xiao Han Liping Yu Jinxiong She Mei Zhang Tao Yang | 2016 | Science China(Life Sciences)2016，59，9： | 5 |
| 8 | Chinese expert consensus on the management of chimeric antigen receptor T cell therapy-associated coagulopathy显示文摘Chimeric antigen receptor T-cell(CAR-T)therapy has greatly improved the disease remission rate and long-term survival rate of patients with relapsed/refractory hematological malignancies.[1-3]Currently,several commercial CAR-T products are available in the market and numerous CAR-T clinical trials have been conducted.Attention should be paid to the safety of CAR-T therapy.The main adverse effects of CAR-T therapy are cytokine release syndrome(CRS)and immune effector cell-associated neurotoxicity syndrome(ICANS).[4] | Heng Mei Fangping Chen Yue Han Ming Hou He Huang Xiaojun Huang Yuhua Li Aibin Liang Qifa Liu Ting Niu Jun Peng Wenbin Qian Yongping Song Jianxiang Wang Ying Wang Depei Wu Kailin Xu Linhua Yang Renchi Yang Lei Zhang Liansheng Zhang Xi Zhang Xiaohui Zhang Weili Zhao Weidong Han Yu Hu | 2022 | Chinese Medical Journal2022，，14： | 4 |
| 9 | Matrix stiffness modulates tip cell formation through the p-PXN-Rac1-YAP signaling axis显示文摘Endothelial tip cell outgrowth of blood-vessel sprouts marks the initiation of angiogenesis which is critical in physiological and pathophysiological procedures.However,how mechanical characteristics of extracellular matrix(ECM)modulates tip cell formation has been largely neglected.In this study,we found enhanced CD31 expression in the stiffening outer layer of hepatocellular carcinoma than in surrounding soft tissues.Stiffened matrix promoted sprouting from endothelial cell(EC)spheroids and upregulated expressions of tip cell-enriched genes in vitro.Moreover,tip cells showed increased cellular stiffness,more actin cytoskeleton organization and enhanced YAP nuclear transfer than stalk and phalanx ECs.We further uncovered that substrate stiffness regulates FAK and Paxillin phosphorylation in focal adhesion of ECs promoting Rac1 transition from inactive to active state.YAP is subsequently activated and translocated into nucleus,leading to increased tip cell specification.p-Paxillin can also loosen the intercellular connection which also facilitates tip cell specification.Collectively our present study shows that matrix stiffness modulates tip cell formation through p-PXN-Rac1-YAP signaling axis,shedding light on the role of mechanotransduction in tip cell formation.This is of special significance in biomaterial design and treatment of some pathological situations. | Yaru Guo Feng Mei Ying Huang Siqin Ma Yan Wei Xuehui Zhang Mingming Xu Ying He Boon Chin Heng Lili Chen Xuliang Deng | 2022 | Bioactive Materials2022，7，1： | 4 |
| 10 | Diagnostic and Prognostic Value of Plasma Factor V Activity and Parameters in Thrombin Generation for Disseminated Intravascular Coagulation in Patients with Hematological Malignancies显示文摘In this study,we used plasma factor V activity and parameters of the thrombin generation test to discuss their diagnostic and prognostic value for disseminated intravascular coagulation (DIC) in patients with hematological malignancies.A total of 164 patients who were diagnosed with hematological malignancies in the Department of Hematology,Union Hospital,between Apr 2014 and Dec.2014 were enrolled in this study.There were 131 patients in the study group and 33 patients in the control group in terms of the laboratory results for DIC.The patients in the study group were divided into a DIC subgroup (n=59) and a non-DIC subgroup (n=72) based on the International Society of Thrombosis and Hemostasis (ISTH) Integral System,and they were divided into four subgroups [score ≤3 (n=35),score=4 (n=37),score=5 (n=47),and score >6 (n=12)] according to ISTH scores.Using 28-day mortality as the endpoint,the patients in the study group were divided into a survival subgroup (n=111) and a non-survival subgroup (n=20).The results showed that the plasma factor V activity was significantly weaker,and lag time and time to peak were significantly shorter in the study group than in the control group (P<0.01).The factor V activity,peak and endogenous thrombin potential (ETP) were significantly decreased in the DIC subgroup as compared with those in the non-DIC subgroup (P<0.01).Among factor V activity,lag time,peak,ETP,and ttPeak,only the factor V activity was significantly decreased in the nonsurvival subgroup compared with the survival subgroup (P<0.01).With the increase in ISTH score,the ETP and peak decreased gradually.The binary logistic regression analysis revealed that PLT,D-dimer,factor V activity and ETP had linear relationship with DIC diagnosed by ISTH Integral System.Using DIC diagnosed by ISTH Integral System as the endpoint,the area under curve (AUC) of factor V activity was found to be similar to that of blood platelet count (PLT) and prothrombin time (PT).In conclusion,factor V activity,ETP and peak had diagnostic value for DIC in patients with hematological malignancies,and only factor V activity had limited prognostic value. | Hai-ming K0U Xiao-ping ZHANG Man-zhi WANG Jun DENG Heng MEI Yu HU | 2019 | Current Medical Science2019，39，4： | 3 |
| 11 | 2022 Chinese expert consensus and guidelines on clinical management of toxicity in anti-CD19 chimeric antigen receptor T-cell therapy for B-cell non-Hodgkin lymphoma显示文摘Adoptive cellular immunotherapy with chimeric antigen receptor(CAR)T cells has emerged as a novel modality for treating relapsed and/or refractory B-cell non-Hodgkin lymphoma(B-NHL).With increasing approval of CAR T-cell products and advances in CAR T cell therapy,CAR T cells are expected to be used in a growing number of cases.However,CAR T-cell-associated toxicities can be severe or even fatal,thus compromising the survival benefit from this therapy.Standardizing and studying the clinical management of these toxicities are imperative.In contrast to other hematological malignancies,such as acute lymphoblastic leukemia and multiple myeloma,anti-CD19 CAR T-cell-associated toxicities in B-NHL have several distinctive features,most notably local cytokine-release syndrome(CRS).However,previously published guidelines have provided few specific recommendations for the grading and management of toxicities associated with CAR T-cell treatment for B-NHL.Consequently,we developed this consensus for the prevention,recognition,and management of these toxicities,on the basis of published literature regarding the management of anti-CD19 CAR T-cell-associated toxicities and the clinical experience of multiple Chinese institutions.This consensus refines a grading system and classification of CRS in B-NHL and corresponding measures for CRS management,and delineates comprehensive principles and exploratory recommendations for managing anti-CD19 CAR T-cell-associated toxicities in addition to CRS. | Ping Li Yang Liu Yun Liang Jian Bo Sujun Gao Yongxian Hu Yu Hu He Huang Xiaojun Huang Hongmei Jing Xiaoyan Ke Jianyong Li Yuhua Li Qifa Liu Peihua Lu Heng Mei Ting Niu Yongping Song Yuqin Song Liping Su Sanfang Tu Jianxiang Wang Depei Wu Zhao Wang Kailin Xu Zhitao Ying Qingming Yang Yajing Zhang Fengxia Shi Bin Zhang Huilai Zhang Xi Zhang Mingfeng Zhao Weili Zhao Xiangyu Zhao Liang Huang Jun Zhu Wenbin Qian Weidong Han Aibin Liang | 2023 | Cancer Biology & Medicine2023，20，2： | 3 |
| 12 | Recombinant Tissue Plasminogen Activator-conjugated Nanoparticles Effectively Targets Thrombolysis in a Rat Model of Middle Cerebral Artery Occlusion显示文摘 | Jun DENG Heng MEI Wei SHI Zhi-qing PANG Bo ZHANG Yao GUO Hua-fang WANG Xin-guo JIANG Yu HU | 2018 | Current Medical Science2018，38，3： | 3 |
| 13 | Lateral Flow Immunoassay for Quantitative Detection of Ractopamine in Swine Urine显示文摘A strip reader based lateral flow immunoassay(LFIA)was established for the rapid and quantitative detection of ractopamine(RAC)in swine urine.The ratio of the optical densities(ODs)of the test line(AT)to that of the control line(AC)was used to effectively minimize interference among strips and sample variations.The linear range for the quantitative detection of RAC was0.2 ng/mL to 3.5 ng/mL with a median inhibitory concentration(IC50)of 0.59±0.06 ng/mL.The limit of detection(LOD)of the LFIA was 0.13 ng/mL.The intra-assay recovery rates were 92.97%,97.25%,and 107.41%,whereas the inter-assay rates were80.07%,108.17%,and 93.7%,respectively. | REN Mei Ling CHEN Xue Lan LI Chao Hui XU Bo LIU Wen Juan XU Heng Yi XIONG Yong Hua | 2014 | Biomedical and Environmental Sciences2014，27，2： | 3 |
| 14 | Enhanced multimodal luminescence and ultrahigh stability Eu^(3+)-doped CsPbBr_(3) glasses for X-ray detection and imaging显示文摘As an emerging scintillation material,metal halide perovskite(CsPbX3)has been deemed the most potentially valuable candidate in X-ray detection and medical imaging.Nevertheless,it is a continuing challenge to implement efficient radioluminescence(RL)with high radiation stability and moisture resistance.Moreover,the optimized luminescence properties and excellent uniformity of CsPbX3 glass are also key points for obtaining perfect X-ray images.Herein,we have successfully precipitated Eu^(3+)-doped CsPbBr_(3)nanocrystals(NCs)with improved photoluminescence quantum yield(≈58.6%)because partial Eu^(3+)entered the perovskite lattice in a robust borosilicate glass matrix by in situ crystallization.The small amount of Eu addition made the lattice of NCs shrink and promoted uniform distribution of CsPbBr_(3)NCs in the glass,which effectively reduced the light scattering of the sample.Subsequently,multimodal RL intensity of the CsPbBr_(3)/CsPbBr_(3):xEu NCs glasses(CPB-0Eu/CPB-xEu)as a function of X-ray dose rate showed a superlinear relationship to the benefit of obtaining satisfactory X-ray images.Also,the outstanding radiation stability and water resistance of CPB-xEu were confirmed due to the protection of the robust glass matrix.Finally,an X-ray imaging system using a CPB-xEu scintillator was constructed,and the spring in the opaque sample was legibly detected under the motivation of X-rays,indicating that CsPbX3 glasses possess extensive application prospects in terms of X-ray detection and medical imaging. | YAO TONG QIN WANG HENG YANG XIAOTING LIU ENROU MEI XIAOJUAN LIANG ZHIJUN ZHANG WEIDONG XIANG | 2021 | Photonics Research2021，9，12： | 2 |
| 15 | Synthesis of Nicotinic Acid by Potassium Dichromate Oxidation显示文摘Nicotinic acid was synthesized from 3 methylpyridine with potassium dichromate as the oxidant. The reaction conditions, the product separation and analysis were studied. The results indicate that under the certain reaction conditions, the yield of nicotinic acid can reach 67.4%, the conversion can reach 99.7% and the selectivity can be as high as 99.8%. The final product has a high purity. | TIAN Mei * , ZHANG Heng bin ** and CAO Xue jingCollege of Chemistry, Jilin University, Changchun 130023, P.R. China | 2002 | Chemical Research in Chinese Universities2002，18，3： | 2 |
| 16 | 2021 Chinese consensus on the diagnosis and management of primary immune thrombocytopenia in pregnancy显示文摘Immune thrombocytopenia(ITP)is an acquired disease characterized by isolated thrombocytopenia,which is one of the most common causes of thrombocytopenia during pregnancy.Women with ITP who have severe thrombocytopenia are at an increased risk for life-threatening obstetric complications.Therefore,we established this consensus statement on the diagnosis and management of ITP during pregnancy(detailed information is available in the Supplementary File,http://gffzz16e312a87a7141a5hx69owk9xkn0w6k0n.ffgz.tsg.suse.edu.cn/A978). | Zhang Xiaohui Chen Fangping Chen Xiequn Cheng Yunfeng Fang Meiyun Feng Jianming Fu Haixia Gao Hong Han Yue He Aili Hou Ming Hu Yu Huang Ruibin Huang Wenrong Jing Zhicheng Kong Peiyan Liang Aibin Liang Meiying Liu Daihong Liu Junling Liu Lin Liu Xiaowei Ma Liangming Mei Heng Ni Heyu Niu Ting Peng Jun Qiao Jianlin Ren Jinhai Song Yongping Tang Liang V Tong Tong Wang Shaoyuan Wang Xin Wang Zhao Wei Hui Wu Depei Wu Guangsheng Xu Caigang Xu Xue Xu Yajing Yang Linhua Yang Renchi Yang Tonghua Yin Chenghong Yu Li Zhang Guangsen Zhang Lei Zhang Liansheng Zhang Xi Zhao Weili Zhao Yongqiang Zhou Daobin Zhou Hu Zhou Zeping Zhu Tienan Wang Jianliu Huang Xiaojun | 2022 | Chinese Medical Journal2022，，8： | 2 |
| 17 | UVA1 irradiation inhibits fibroblast proliferation and alleviates pathological changes of scleroderma in a mouse model显示文摘The purpose of the present study was to compare the effects of different doses of ultraviolet radiation A1 (UVA1) on human fibroblast proliferation and collagen level in a mouse model of scleroderma,so as to identify appropriate irradiation doses for clinical treatment of scleroderma.Monolayer from human fibroblasts was cultured in vitro,and a mouse model of scleroderma was established by subcutaneous injection of 100 μL of 400 μg/mL bleomycin into the back of BALB/c mice for 4 weeks.The mouse models and human fibroblasts were divided into UVA1exposed (100,60 and 20 J/cm 2) and UVA-unexposed groups.At 0,24 and 48 h after exposure,cell proliferation and levels of hydroxyproline and collagen were detected.UVA1 irradiation was performed 3 times weekly for 10 weeks,and the pathological changes of skin tissues,skin thickness and collagen level were observed after phototherapy.Cell proliferation and the levels of hydroxyproline and collagen were inhibited after phototherapy,and there was a significant difference between the UVA1-exposed cells and UVA1-unexposed cells (P < 0.001).In addition,UVA1 phototherapy improved dermal sclerosis and softened the skin,and there were significant differences between the high-dose UVA1 group and the model group,and the negative group (P < 0.05).It is concluded that UVA1 radiation can reduce cell proliferation,and decrease hydroxyproline and collagen levels in a dose-dependent manner in vitro.High-dose UVA1 phototherapy has marked therapeutic effect on scleroderma in the mouse model.Decreased collagen level may be related to the reduced number and activity of cells,as well as inhibition of collagen synthesis. | Mei Ju Kun Chen Baozhu Chang Heng Gu | 2012 | The Journal of Biomedical Research2012，26，2： | 2 |
| 18 | Potent in vitro Interference of Fleroxacin in DNA-binding,Unwinding and ATPase Activities of Bloom Helicase显示文摘Objective To study the effect of fleroxacin(FLRX) on biological properties of Bloom(BLM) helicase catalytic core(BLM 642-1290 helicase) in vitro and the molecular mechanism of interaction between the two molecules.Methods DNA-binding and unwinding activities of BLM 642-1290 helicase were assayed by fluorescence polarization and gel retardation assay under conditions that the helicase was subjected to different concentrations of FLRX.Effect of FLRX on helicase ATPase activity was analyzed by phosphorus-free assay based on a colorimetric estimation of ATP hydrolysis-produced inorganic phosphate.Molecular mechanism ofinteractionbetween thetwomoleculeswasassayedbyultraviolet andfluorescence spectra.Results The DNA unwinding and ATPase activities of BLM 642-1290 helicase were inhibited whereas the DNA-binding activity was promoted in vitro.A BLM-FLRX complex was formed through one binding site,electrostatic and hydrophobic interaction force.Moreover,the intrinsic fluorescence of the helicase was quenched by FLRX as a result of non-radioactive energy transfer.The biological activity of helicase was affected by FLRX,which may be through an allosteric mechanism and stabilization of enzyme conformation in low helicase activity state,disruption of the coupling of ATP hydrolysis to unwinding,and blocking helicase translocation on DNA strands.Conclusion FLRX may affect the biological activities and conformation of BLM 642-1290 helicase,and DNA helicase may be used as a promising drug target for some diseases. | LUO Heng XU Hou Qiang CHEN Xiang DING Mei YANG Qi Xin LI Kun | 2013 | Biomedical and Environmental Sciences2013，26，4： | 2 |
| 19 | Panda X-III: Searching for neutrinoless double beta decay with high pressure ^(136)Xe gas time projection chambers显示文摘Searching for the neutrinoless double beta decay(NLDBD)is now regarded as the topmost promising technique to explore the nature of neutrinos after the discovery of neutrino masses in oscillation experiments.Panda X-III(particle and astrophysical xenon experiment III)will search for the NLDBD of136Xe at the China Jin Ping Underground Laboratory(CJPL).In the first phase of the experiment,a high pressure gas Time Projection Chamber(TPC)will contain 200 kg,90%136Xe enriched gas operated at10 bar.Fine pitch micro-pattern gas detector(Microbulk Micromegas)will be used at both ends of the TPC for the charge readout with a cathode in the middle.Charge signals can be used to reconstruct the electron tracks of the NLDBD events and provide good energy and spatial resolution.The detector will be immersed in a large water tank to ensure~5 m of water shielding in all directions.The second phase,a ton-scale experiment,will consist of five TPCs in the same water tank,with improved energy resolution and better control over backgrounds. | Xun Chen Chang Bo Fu Javier Galan Karl Giboni Franco Giuliani Ling Hui Gu Ke Han Xiang Dong Ji Heng Lin Jiang Lai Liu Kai Xiang Ni Hiroki Kusano Xiang Xiang Ren Shao Bo Wang Yong Yang Dan Zhang Tao Zhang Li Zhao Xiang Ming Sun Shou Yang Hu Si Yu Jian Xing Long Li Xiao Mei Li Hao Liang Huan Qiao Zhang Ming Rui Zhao Jing Zhou Ya Jun Mao Hao Qiao Si Guang Wang Ying Yuan Meng Wang Amir N.Khan Neill Raper Jian Tang Wei Wang Jia Ning Dong Chang Qing Feng Cheng Li Jian Bei Liu Shu Bin Liu Xiao Lian Wang Dan Yang Zhu Juan F.Castel Susana Cebri'an Theopisti Dafni Javier G.Garza Igor G.Irastorza Francisco J.Iguaz Gloria Luz ′on Hector Mirallas Stephan Aune Eric Berthoumieux Yann Bedfer Denis Calvet Nicole d'Hose Alain Delbart Maria Diakaki Esther Ferrer-Ribas Andrea Ferrero Fabienne Kunne Damien Neyret Thomas Papaevangelou Franck Sabatie Maxence Vanderbroucke AnDi Tan Wick Haxton Yuan Mei Chinorat Kobdaj Yu-Peng Yan | 2017 | Science China(Physics,Mechanics & Astronomy)2017，60，6： | 2 |
| 20 | A new approach to the synthesis of α-chlorocycloalkanones显示文摘α-Chlorocycloalkanones(Ⅳ) were synthesized from cycloalkanones via potassiumα-oxocycloalkylsulfonates(Ⅱ).A two-step mechanism was proposed to explain the formation ofⅣfromⅡ. | Xing Hai Li Xin Ling Yang Xiao Mei Liang Zhen Peng Kai Yun Ling Fu Heng Chen Dao Quan Wang | 2007 | Chinese Chemical Letters2007，18，12： | 1 |
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