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| 1 | RNA-Directed DNA Methylation Is Involved in Regulating Photoperiod-Sensitive Male Sterility in Rice显示文摘光周期敏感的男绝育(PSMS ) 是为混合米饭繁殖的珍贵 germplasm。最近,我们克隆 pms3,控制 PSMS 的一个地点,它编码称为在长天的条件下面为米饭植物的正常男富饶要求的 LDMAR 的长非编码的 RNA。在在 PSMS 米饭(Nongken 58S ) 相对的 LDMAR 的倡导者的增加的 methylation 野类型(Nongken 58 ) 减少了在长天的条件下面导致男绝育的 LDMAR 的表示。在这研究,我们鉴别 siRNA 在比在 Nongken 58 更充满 Nongken 58S 的 LDMAR 倡导者区域说出 PsiLDMAR。我们证明 PsiLDMAR 多半从 AK111270 被导出,与它让 110 底与 LDMAR 的 5 结束重叠的 3 结束从 LDMAR 倡导者的感觉海滨获得的一个抄本。在 Nongken 58S 的 Overexpressing AK111270 极大地充实 PsiLDMAR,它在 LDMAR 倡导者导致了指导 RNA 的 DNA methylation 并且镇压 LDMAR 的表示。在 Nongken 58S 的 LDMAR 的减小为富饶恢复改变了批评的天长度并且在短天的条件下面推迟了富饶恢复。这结果为 PSMS 增加了我们分子的机制的理解。 | Jihua Ding Jianqiang Shen Hailiang Mao Weibo Xie Xianghua Li Qifa Zhang | 2012 | Molecular Plant2012，5，6： | 33 |
| 2 | Thermal entanglement in a mixed-spin Heisenberg XXZ model under a nonuniform external magnetic field显示文摘The thermal entanglement in (1/2,1) mixed-spin Heisenberg XXZ model is investigated under an external nonuniform magnetic field. In the uniform magnetic field system,the critical magnetic field Bc and critical temperature Tc are increased by increasing the anisotropic parameter k. The degree of magnetic field b plays an important role in improving the critical temperature and enlarging the region of entan-glement in the nonuniform magnetic field system. | WANG Fei JIA HongHui ZHANG HaiLiang ZHANG XueAo CHANG ShengLi | 2009 | Science China(Physics,Mechanics & Astronomy)2009，52，12： | 13 |
| 3 | Genome-Wide Analysis of the Sus Gene Family in Cotton显示文摘蔗糖 synthase (怀疑) 是在植物蔗糖新陈代谢的关键酶。在棉花,怀疑(EC 2.4.1.13 ) 是降级的主要的酶从种子上衣的韧皮部进口进棉花纤维的蔗糖。这研究示威了 Gossypium arboreum L. 的染色体, G。raimondii Ulbr,和 G。hirsutum L. ,包含了 8, 8,和 15 分别地怀疑基因。他们的结构的组织,种系发生的关系,和表示侧面被描绘。genomic 和编码序列的比较识别了哪个高度被保存在之间的多重 introns,数字和位置双并且 allotetraploid 棉花种类。大多数种系发生的 clades 从所有三种包含了序列,建议 tetraploid G 的 Sus 基因。hirsutum 它的双祖先源于那些。一个人怀疑组(怀疑我) 在棉花期间经历了扩大进化。表示分析显示大多数怀疑基因差别在各种各样的纸巾被表示并且在棉花纤维房间有发展依赖者表示侧面。一样的 orthologous 组的成员在所有三种有很类似的表示模式。这些结果提供新卓见进棉花的进化在纤维生长和开发期间怀疑基因家庭,和卓见进它的成员的生理的功能。 | Changsong Zou Cairui Lu Haihong Shang Xinrui Jing Hailiang Cheng Youping Zhang Guoli Song | 2013 | Journal of Integrative Plant Biology2013，55，7： | 10 |
| 4 | Microbial diversity in cold seep sediments from the northern South China Sea显示文摘华南海(SCS ) 是最大的西方的和平的边缘的海。然而,微生物引起的研究从来没在寒冷被执行过渗出在 SCS 的沉积。在 2004, SONNE 177 巡航发现二冷渗出有在北 SCS 的不同的水深度的区域。在水深度在 3000 m 附近的地方, Haiyang 4 区域已经为在 seafloor 上的活跃渗出被证实了,例如微生物引起的地席, authigenic 碳酸盐外壳和瓣鳃类。我们在从这感冒收集的一个 5.55-m 沉积核心调查了微生物引起的丰富和差异渗出区域。一条综合途径包括地球化学和 16S rRNA 基因被采用种系发生的分析。这里,我们证明与沉积核心的地球化学侧面一起的微生物引起的丰富和差异揭示了在硫酸盐减小和甲烷氧化之间的 a coupled 反应。直接计数结果显示出的氮蒽橘子那微生物引起的丰富从 105 ~ 106 cells/g 沉积(湿重量)。丰富的深度相关的变化显示出象甲烷集中侧面的一样的趋势。种系发生的分析显示了减少硫酸盐的细菌和厌氧的氧化甲烷的 archaea 的存在。差异在表面是高得多的,但是处于沉积的 geochemical 条件响应变化与深度严厉地减少了,例如甲烷,硫酸盐集中和全部的器官的碳。海洋的 Benthic 组 B, Chloroflexi 和 JS1 分别地是 archaeal 和细菌的图书馆的占优势的 phylotypes。 | Yong Zhang Xin Su Fang Chen Yuanyuan Wang Lu Jiao Hailiang Dong Yongyang Huang Hongchen Jiang | 2012 | Geoscience Frontiers2012，3，3： | 9 |
| 5 | Transcriptome analysis reveals long noncoding RNAs involved in fiber development in cotton (Gossypium arboreum)显示文摘Long noncoding RNAs(lnc RNAs)play important roles in various biological regulatory processes in yeast,mammals,and plants.However,no systematic identification of lnc RNAs has been reported in Gossypium arboreum.In this study,the strand-specific RNA sequencing(ss RNA-seq)of samples from cotton fibers and leaves was performed,and lnc RNAs involved in fiber initiation and elongation processes were systematically identified and analyzed.We identified 5,996 lnc RNAs,of which 3,510 and 2,486 can be classified as long intergenic noncoding RNAs(linc RNAs)and natural antisense transcripts(lnc NAT),respectively.Linc RNAs and lnc NATs are similar in many aspects,but have some differences in exon number,exon length,and transcript length.Expression analysis revealed that 51.9%of linc RNAs and 54.5%of lnc NATs transcripts were preferentially expressed at one stage of fiber development,and were significantly highly expressed than protein-coding transcripts(21.7%).During the fiber and rapid elongation stages,rapid and dynamic changes in lnc RNAs may contribute to fiber development in cotton.This work describes a set of lnc RNAs that are involved in fiber development.The characterization and expression analysis of lnc RNAs will facilitate future studies on their roles in fiber development in cotton. | Changsong Zou Qiaolian Wang Cairui Lu Wencui Yang Youping Zhang Hailiang Cheng Xiaoxu Feng Mtawa Andrew Prosper Guoli Song | 2016 | Science China(Life Sciences)2016，59，2： | 9 |
| 6 | GABAergic Abnormalities Associated with Sensorimotor Corticostriatal Community Structural Deficits in ErbB4 Knockout Mice and First-Episode Treatment-Naive Patients with Schizophrenia显示文摘The current study was designed to explore how disruption of specific molecular circuits in the cerebral cortex may cause sensorimotor cortico-striatal community structure deficits in both a mouse model and patients with schizophrenia.We used prepulse inhibition(PPI)and brain structural and diffusion MRI scans in 23 mice with conditional ErbB4 knockout in parvalbumin interneurons and 27 matched controls.Quantitative real-time PCR was used to assess the differential levels of GABA-related transcripts in brain regions.Concurrently,we measured structural and diffusion MRI and the cumulative contribution of risk alleles in the GABA pathway genes in firstepisode treatment-naı¨ve schizophrenic patients(n=117)and in age-and sex-matched healthy controls(n=86).We present the first evidence of gray and white matter impairment of right sensorimotor cortico-striatal networks and reproduced the sensorimotor gating deficit in a mouse model of schizophrenia.Significant correlations between gray matter volumes(GMVs)in the somatosensory cortex and PPI as well as glutamate decarboxylase 1 mRNA expression were found in controls but not in knockout mice.Furthermore,these findings were confirmed in a human sample in which we found significantly decreased gray and white matter in sensorimotor cortico-striatal networks in schizophrenic patients.The psychiatric risk alleles of the GABA pathway also displayed a significant negative correlation with the GMVs of the somatosensory cortex in patients.Our study identified that ErbB4 ablation in parvalbumin interneurons induced GABAergic dysregulation,providing valuable mechanistic insights into the sensorimotor cortico-striatal community structure deficits associated with schizophrenia. | Chengcheng Zhang Peiyan Ni Yikang Liu Yang Tian Jinxue Wei Bo Xiang Liansheng Zhao Xiaojing Li Xiaohong Ma Wei Deng Wanjun Guo Rongjun Ni Yamin Zhang Qiang Wang Hailiang Huang Nanyin Zhang Tao Li | 2020 | Neuroscience Bulletin2020，36，2： | 9 |
| 7 | Large time behavior of solutions to 3D compressible Navier-Stokes-Poisson system显示文摘We consider the three-dimensional compressible Navier-Stokes-Poisson system where the electric field of the internal electrostatic potential force is governed by the self-consistent Poisson equation.If the Fourier modes of the initial data are degenerate at the low frequency or the initial data decay fast at spatial infinity,we show that the density converges to its equilibrium state at the L 2-rate (1+t)(-7/4) or L ∞-rate (1+t)(-5/2),and the momentum decays at the L 2-rate (1+t)(-5/4) or L ∞-rate (1+t)(-2).These convergence rates are shown to be optimal for the compressible Navier-Stokes-Poisson system. | LI HaiLiang ZHANG Ting | 2012 | Science China Mathematics2012，55，1： | 7 |
| 8 | The essential ability of sparse reconstruction of different compressive sensing strategies显示文摘We show the essential ability of sparse signal reconstruction of different compressive sensing strategies,which include the L1 regularization,the L0 regularization(thresholding iteration algorithm and OMP algorithm),the Lq(0 < q ≤ 1) regularizations,the Log regularization and the SCAD regularization.Taking phase diagram as the basic tool for analysis,we find that(i) the solutions of the L0 regularization using hard thresholding algorithm and OMP algorithm are similar to those of the L1 regularization;(ii) the Lq regularization with the decreasing value of q,the Log regularization and the SCAD regularization can attain sparser solutions than the L1 regularization;(iii) the L1/2 regularization can be taken as a representative of the Lq(0 < q < 1) regularizations.When 1/2 < q < 1,the L1/2 regularization always yields the sparsest solutions and when 0 < q < 1/2 the performance of the regularizations takes no significant difference.The results of this paper provide experimental evidence for our previous work. | ZHANG Hai LIANG Yong GOU HaiLiang XU ZongBen | 2012 | Science China(Information Sciences)2012，55，11： | 7 |
| 9 | Availability modeling for periodically inspection system with different lifetime and repair-time distribution显示文摘Availability is a main feature of design and operation of all engineering system. Recently,availability evaluation of periodical inspection systems with different structures is at the center of attention due to the wide application in engineering. In this paper, an analytical and probabilistic availability model for periodical inspection system is proposed by a new recursively algorithm,which can achieve limiting average availability and instantaneous availability of periodical inspection system under arbitrary lifetime and repair-time distributions. Then three application examples are presented, the systems lifetime and repair-time are respectively fellow exponential/exponential,Weibull/normal and Weibull/lognormal distribution. Finally, a Weibull/lognormal system is studied to analyze the dynamic relationship between inspection period and availability. The results indicate that the proposed approach can provide the technology support for improving system availability and determining reasonable inspection period. | Junliang LI Yueliang CHEN Yong ZHANG Hailiang HUANG | 2019 | Chinese Journal of Aeronautics2019，32，7： | 7 |
| 10 | Electroacupuncture attenuates neuropathic pain after brachial plexus injury显示文摘Electroacupuncture has traditionally been used to treat pain, but its effect on pain following brachial plexus injury is still unknown. In this study, rat models of an avulsion injury to the left brachial plexus root(associated with upper-limb chronic neuropathic pain) were given electroacupuncture stimulation at bilateral Quchi(LI11), Hegu(LI04), Zusanli(ST36) and Yanglingquan(GB34). After electroacupuncture therapy, chronic neuropathic pain in the rats' upper limbs was significantly attenuated. Immunofluorescence staining showed that the expression of β-endorphins in the arcuate nucleus was significantly increased after therapy. Thus, experimental findings indicate that electroacupuncture can attenuate neuropathic pain after brachial plexus injury through upregulating β-endorphin expression. | Shenyu Zhang Hailiang Tang Junming Zhou Yudong Gu | 2014 | Neural Regeneration Research2014，9，14： | 7 |
| 11 | A 12-kyr record of microbial branched and isoprenoid tetraether index in Lake Qinghai,northeastern Qinghai-Tibet Plateau:Implications for paleoclimate reconstruction显示文摘Branched and Isoprenoid Tetraether(BIT) index was considered as a proxy for terrestrial organic matter input in lake sediments, based on the assumption that branched glycerol dialkyl glycerol tetraethers(b GDGTs) are mainly derived from terrestrial soils. However, mounting evidences have showed that the in situ production of b GDGTs is widespread in lakes, challenging BIT as a reliable terrestrial input proxy. Recently, BIT has been proven to be a reliable proxy for paleohydrology in a small crater lake(Lake Challa) in accordance with a different mechanism. However, the response of BIT to paleohydrology variation may differ for different lakes. In this study, we investigate the variations in the BIT index and the concentrations of its related GDGTs in a 12-ka sediment core from Lake Qinghai, in combination with our previous results for surface sediments. We find that variations in BIT strongly depend on the concentration of crenarchaeol in both surface and ancient sediments of this lake, whereas b GDGT concentration varies much less remarkably. Considering that crenarchaeol production is positively correlated with water depth in Lake Qinghai, water depth may exert negative control on the BIT index in this lake. This case is inconsistent with the positive relationship between BIT and lake levels or rainfall intensity reported for Lake Challa, suggesting that the response of BIT to local paleohydrology is site specific in lacustrine systems. Hence, the application of sedimentary BIT as a paleohydrological proxy in a specific lake requires caution before confirming the environmental controls on BIT in that lake. | WANG HuanYe DONG HaiLiang ZHANG ChuanLun JIANG HongChen LIU WeiGuo | 2016 | Science China Earth Sciences2016，59，5： | 6 |
| 12 | The superiority of multi-trait models with genotype-by-environment interactions in a limited number of environments for genomic prediction in pigs显示文摘Background: Different production systems and climates could lead to genotype-by-environment(G × E) interactions between populations, and the inclusion of G × E interactions is becoming essential in breeding decisions. The objective of this study was to investigate the performance of multi-trait models in genomic prediction in a limited number of environments with G × E interactions.Results: In total, 2,688 and 1,384 individuals with growth and reproduction phenotypes, respectively, from two Yorkshire pig populations with similar genetic backgrounds were genotyped with the PorcineSNP80 panel.Single-and multi-trait models with genomic best linear unbiased prediction(GBLUP) and BayesC π were implemented to investigate their genomic prediction abilities with 20 replicates of five-fold cross-validation.Our results regarding between-environment genetic correlations of growth and reproductive traits(ranging from 0.618 to 0.723) indicated the existence of G × E interactions between these two Yorkshire pig populations. For single-trait models, genomic prediction with GBLUP was only 1.1% more accurate on average in the combined population than in single populations, and no significant improvements were obtained by BayesC π for most traits. In addition, single-trait models with either GBLUP or BayesC π produced greater bias for the combined population than for single populations. However, multi-trait models with GBLUP and BayesC π better accommodated G × E interactions,yielding 2.2% – 3.8% and 1.0% – 2.5% higher prediction accuracies for growth and reproductive traits, respectively,compared to those for single-trait models of single populations and the combined population. The multi-trait models also yielded lower bias and larger gains in the case of a small reference population. The smaller improvement in prediction accuracy and larger bias obtained by the single-trait models in the combined population was mainly due to the low consistency of linkage disequilibrium between the two populations, which also caused the BayesC π method to always produce the largest standard error in marker effect estimation for the combined population.Conclusions: In conclusion, our findings confirmed that directly combining populations to enlarge the reference population is not efficient in improving the accuracy of genomic prediction in the presence of G × E interactions, while multi-trait models perform better in a limited number of environments with G × E interactions. | Hailiang Song Qin Zhang Xiangdong Ding | 2021 | Journal of Animal Science and Biotechnology2021，12，1： | 6 |
| 13 | Cooperation of invariant NKT cells and CD4+CD25+ T regulatory cells in prevention of autoimmune diabetes in non-obese diabetic mice treated with α- galactosylceramide显示文摘 | Weipeng Li Fang Ji Yong Zhang Ying Wang Neng Yang Hailiang Ge Fuqing Wang | 2008 | Acta Biochimica et Biophysica Sinica2008，40，5： | 5 |
| 14 | Selectivity regulation of CO2 electroreduction through contact interface engineering on superwetting Cu nanoarray electrodes显示文摘Electrocatalytic CO2 reduction is a promising way to mitigate the urgent energy and environmental issues, but how to increase the selectivity for desired product among multiple competi ng reaction pathways remains a bottle neck. Here, we demonstrate that engineering the gas-liquid-solid contact interface on the electrode surface could tailor the selectivity of CO2 reducti on and meanwhile suppress H2 production through regulated reacti on kin etics. Specifically, polytetrafl uoroethyle ne (PTFE) was utilized to modify a Cu nano array electrode as an example, which is able to cha nge the electrode surface from aerophobic to aerophilic state. The en riched nano-tunnels of the Cu nano array electrode can facilitate CO2 transportation and pin gaseous products on the electrode surface. The latter is believed to be the reason that boosts the Faradaic efficie ncy of liquid products by 67% and limits the H2 producti on to less than half of before. This in terface engin eeri ng strategy also lowered H2O (proton) affinity, therefore promoting CO and HCOOH production. Engineering the electrode contact interface controls the reaction kinetics and the selectivity of products, which should be inspiring for other electrochemical reactions. | Zhao Cai Yusheng Zhang Yuxin Zhao Yueshen Wu Wenwen Xu Xuemei Wen Yang Zhong Ying Zhang Wen Liu Hailiang Wang Yun Kuang Xiaoming Sun | 2019 | Nano Research2019，12，2： | 5 |
| 15 | Changes of annual accumulated temperature over Southern China during 1960-2011显示文摘The spatial and temporal variations of ≥10°C annual accumulated temperature(AAT10) were analyzed by using the linear trend line method, cumulative anomaly method and the multiple linear regression model(MLRM) interpolation method based on the daily meteorological observation data from 104 meteorological stations in Southern China and surrounding 39 meteorological stations from 1960 to 2011. The results show that:(1) From time scale point of view, the climatic trend of the AAT10 increased with an average of 7.54°C/decade in Southern China since 1960. The area of AAT10<6000°C decreased from 1960 to 2011, and the area of 6000°C8000°C increased from 1960 to 2011.(2) From spatial scale point of view, the AAT10 in Southern China reduced with increasing latitude and reduced with increasing altitude. The proportion of the area with 5000°C< AAT10<8000°C accounted for 70% of the study area, followed by the area of 4000°C8000°C was the least. Climate trend rate of the AAT10 at 99% of the meteorological stations was greater than zero, which indicated that the AAT10 increased significantly in the central Yunnan province, southern Guangdong province as well as Hainan Island.(3) Comparison of period A(1960–1989) and period B(1980–2011) with the change of temperature zones shows that the boundaries of cool temperate zone, mid-temperate zone and warm temperate zone shifted northward and shrank westward. The northern boundary of north subtropical zone and mid-subtropical zone shifted northward gradually by over 0.5° and 0.5° latitude, respectively. The western part of northern boundary of south subtropical zone and marginal tropical zone shifted northward by 0.2° and 0.4° latitude, respectively. The change of temperature zones was expanded to high altitude and latitude.(4) The increase of the AAT10 is conducive to the production of tropical crops planted, which will increase the planting area that was suitable for tropical crops, and expand the planting boundaries to high latitude and high altitude. | DAI Shengpei LI Hailiang LUO Hongxia ZHAO Yifei ZHANG Kexin | 2015 | Journal of Geographical Sciences2015，25，10： | 4 |
| 16 | Diversity of microbial plankton across the Three Gorges Dam of the Yangtze River,China显示文摘长江的三个峡水坝(TGD ) ,中国,在世界上是最大的灌溉和水力发电的工程工程之一。效果巨大人造象动物志和 macrophyte 上的 TGD 一样的工程是明显的,主要通过水动力学和流动模式的变化;然而,微生物怎么对如此的变化作出回应,是不太清楚的。这研究被瞄准在不同季节和地点在微生物引起的差异检验差别(在前面并且在 TGD 后面) 。另外,依附粒子、免费生活的社区之间的差别也被检验。在水列在后面并且在 TGD 前面的全部、潜在地活跃的微生物的社区结构与基于 DNA 、基于 RNA 的 16S rRNA 基因被分析在三个不同季节的种系发生的途径。16S rRNA 基因的克隆图书馆从提取 DNA 在扩大以后被准备并且为一些样品,在从提取 rRNA 准备 cDNA 以后。差别在不同季节并且在免费生活、依附粒子的社区之间在地点之间被观察。细菌并且 archaeal 社区比在冬季在夏天是更多样的,由于在夏天的更高滋养的层次和更温暖的温度比在冬季。依附粒子的微生物比免费生活的社区更多样,可能因为在粒子的更高滋养的层次和异构的 geochemical 微型环境。在细菌的社区结构的空间变化被观察,即,在 TGD 后面的水水库(在上游) 比在水坝前面招待了更多样的细菌的人口(下游) ,因为沉积和水从的多样的来源对水库在上游。这些结果在水坝建设影响的河生态系统为我们对环境变化的微生物引起的社区的回答的理解有重要含意。 | Shang Wang Raymond M. Dong Christina Z. Dong Liuqin Huang Hongchen Jiang Yuli Wei Liang Feng Deng Liu Guifang Yang Chuanlun Zhang Hailiang Dong | 2012 | Geoscience Frontiers2012，3，3： | 4 |
| 17 | KLF4 is a tumor suppressor in anaplastic meningioma stem-like cells and human rneningiomas显示文摘 | Hailiang Tang Hongda Zhu Xuanchun Wang Lingyang Hua Jingrun Li Qing Xie Xiancheng Chen Tao Zhang Ye Gong | 2017 | Journal of Molecular Cell Biology2017，9，4： | 3 |
| 18 | Application of CUSA Excel ultrasonic aspiration system in resection of skull base meningiomas显示文摘Background: Effective methods for managing patients with solitary pulmonary nodules(SPNs) depend critically on the predictive probability of malignancy.Methods: Between July 2009 and June 2011, data on gender, age, cancer history, tumor familial history, smoking status, tumor location, nodule size, spiculation, calcification, the tumor border, and the final pathological diagnosis were collected retrospectively from 154 surgical patients with an SPN measuring 3-30 mm. Each final diagnosis was compared with the probability calculated by three predicted models—the Mayo, VA, and Peking University(PU) models. The accuracy of each model was assessed using area under the receiver operating characteristics(ROC) and calibration curves.Results: The area under the ROC curve of the PU model [0.800; 95% confidence interval(CI): 0.708-0.891] was higher than that of the Mayo model(0.753; 95% CI: 0.650-0.857) or VA model(0.728; 95% CI: 0.623-0.833); however, this finding was not statistically significant. To varying degrees, calibration curves showed that all three models overestimated malignancy.Conclusions: The three predicted models have similar accuracy for prediction of SPN malignancy, although the accuracy is not sufficient. For Chinese patients, the PU model may has greater predictive power.Background: Here, we introduced our short experience on the application of a new CUSA Excel ultrasonic aspiration system, which was provided by Integra Lifesciences corporation, in skull base meningiomas resection.Methods: Ten patients with anterior, middle skull base and sphenoid ridge meningioma were operated using the CUSA Excel ultrasonic aspiration system at the Neurosurgery Department of Shanghai Huashan Hospital from August 2014 to October 2014. There were six male and four female patients, aged from 38 to 61 years old(the mean age was 48.5 years old). Five cases with tumor located at anterior skull base, three cases with tumor on middle skull base, and two cases with tumor on sphenoid ridge.Results: All the patents received total resection of meningiomas with the help of this new tool, and the critical brain vessels and nerves were preserved during operations. All the patients recovered well after operation.Conclusions: This new CUSA Excel ultrasonic aspiration system has the advantage of preserving vital brain arteries and cranial nerves during skull base meningioma resection, which is very important for skull base tumor operations. This key step would ensure a well prognosis for patients. We hope the neurosurgeons would benefit from this kind of technique.Background: The purposes of this study were to explore the effects of high mobility group protein box 1(HMGB1) gene on the growth, proliferation, apoptosis, invasion, and metastasis of glioma cells, with an attempt to provide potential therapeutic targets for the treatment of glioma. Methods: The expressions of HMGB1 in glioma cells(U251, U-87 MG and LN-18) and one control cell line(SVG p12) were detected by real time PCR and Western blotting, respectively. Then, the effects of HMGB1 on the biological behaviors of glioma cells were detected: the expression of HMGB1 in human glioma cell lines U251 and U-87 MG were suppressed using RNAi technique, then the influences of HMGB1 on the viability, cycle, apoptosis, and invasion abilities of U251 and U-87 MG cells were analyzed using in a Transwell invasion chamber. Also, the effects of HMGB1 on the expressions of cyclin D1, Bax, Bcl-2, and MMP 9 were detected. Results: As shown by real-time PCR and Western blotting, the expression of HMGB1 significantly increased in glioma cells(U251, U-87 MG, and LN-18) in comparison with the control cell line(SVG p12); the vitality, proliferation and invasive capabilities of U251 and U-87 MG cells in the HMGB1 siR NA-transfected group were significantly lower than those in the blank control group and negative control(NC) siR NA group(P<0.05) but showed no significant difference between the blank control group and NC siR NA group. The percentage of apoptotic U251 and U-87 MG cells was significantly higher in the HMGB1 siR NA-transfected group than in the blank control group and NC siR NA group(P<0.05) but was similar between the latter two groups. The HMGB1 siR NA-transfected group had significantly lower expression levels of Cyclin D1, Bcl-2, and MMP-9 protein in U251 and U-87 MG cells and significantly higher expression of Bax protein than in the blank control group and NC siR NA group(P<0.05); the expression profiles of cyclin D1, Bax, Bcl-2, and MMP 9 showed no significant change in both blank control group and NC siR NA group. Conclusions: HMGB1 gene may promote the proliferation and migration of glioma cells and suppress its effects of apoptosis. Inhibition of the expression of HMGB1 gene can suppress the proliferation and migration of glioma cells and promote their apoptosis. Our observations provided a new target for intervention and treatment of glioma. | Hailiang Tang Haishi Zhang Qing Xie Ye Gong Mingzhe Zheng Daijun Wang Hongda Zhu Xiancheng Chen Liangfu Zhou | 2014 | Chinese Journal of Cancer Research2014，26，6： | 3 |
| 19 | Nondestructive assessment of quality of Nanfeng mandarin fruit by a portable near infrared spectroscopy显示文摘A portable near infrared spectroscopy system was developed for assessing the quality of Nanfeng mandarin fruit.One hundred and fifty-three Nanfeng mandarin samples were used to measure the performance of the system.Several pretreatment methods were adopted to process the spectra.Then Support Vector Machine(SVM),Back Propagation Neural Network(BPNN)and Partial Least Square(PLS)were used to build models for soluble solids content(SSC),titratable acidity(TA),vitamin C and surface color.The best results were obtained by SVM.The correlation coefficient(R)and root mean square error of prediction(RMSEP)were(0.93,0.65°Brix),(0.66,0.09%),(0.81,2.7mg/100g)and(0.57,0.81)for SSC,TA,vitamin C and color,respectively.The results demonstrated that the portable near infrared spectroscopy was feasible for determining the Nanfeng mandarin quality nondestructively. | Sun Xudong Zhang Hailiang Liu Yande | 2009 | International Journal of Agricultural and Biological Engineering2009，2，1： | 3 |
| 20 | Sensing and lasing applications of whispering gallery mode microresonators显示文摘 | Yu Zheng Zhifang Wu Perry Ping Shum Zhilin Xu Gerd Keiser Georges Humbert Hailiang Zhang Shuwen Zeng Xuan Quyen Dinh | 2018 | Opto-Electronic Advances2018，1，9： | 3 |
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