|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Auranofin mitigates systemic iron overload and induces ferroptosis via distinct mechanisms显示文摘Iron homeostasis is essential for health;moreover,hepcidin-deficiency results in iron overload in both hereditary hemochromatosis and iron-loading anemia.Here,we identified iron modulators by functionally screening hepcidin agonists using a library of 640 FDA-approved drugs in human hepatic Huh7 cells.We validated the results in C57BL/6J mice and a mouse model of hemochromatosis(Hfe^(−/−)mice).Our screen revealed that the anti-rheumatoid arthritis drug auranofin(AUR)potently upregulates hepcidin expression.Interestingly,we found that canonical signaling pathways that regulate iron,including the Bmp/Smad and IL-6/Jak2/Stat3 pathways,play indispensable roles in mediating AUR’s effects.In addition,AUR induces IL-6 via the NF-κB pathway.In C57BL/6J mice,acute treatment with 5 mg/kg AUR activated hepatic IL-6/hepcidin signaling and decreased serum iron and transferrin saturation.Whereas chronically treating male Hfe^(−/−)mice with 5 mg/kg AUR activated hepatic IL-6/hepcidin signaling,decreasing systemic iron overload,but less effective in females.Further analyses revealed that estrogen reduced the ability of AUR to induce IL-6/hepcidin signaling in Huh7 cells,providing a mechanistic explanation for ineffectiveness of AUR in female Hfe^(−/−)mice.Notably,high-dose AUR(25 mg/kg)induces ferroptosis and causes lipid peroxidation through inhibition of thioredoxin reductase(TXNRD)activity.We demonstrate the ferroptosis inhibitor ferrostatin significantly protects liver toxicity induced by highdose AUR without comprising its beneficial effect on iron metabolism.In conclusion,our findings provide compelling evidence that TXNRD is a key regulator of ferroptosis,and AUR is a novel activator of hepcidin and ferroptosis via distinct mechanisms,suggesting a promising approach for treating hemochromatosis and hepcidin-deficiency related disorders. | Lei Yang Hao Wang Xiang Yang Qian Wu Peng An Xi Jin Weiwei Liu Xin Huang Yuzhu Li Shiyu Yan Shuying Shen Tingbo Liang Junxia Min Fudi Wang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 5 |
| 2 | Microwave-induced Apoptosis and Cytotoxicity of NK Cells through ERK1/2 Signaling显示文摘Objective To investigate microwave-induced morphological and functional injury of natural killer(NK) cells and uncover their mechanisms. Methods NK-92 cells were exposed to 10, 30, and 50 m W/cm^2 microwaves for 5 min. Ultrastructural changes, cellular apoptosis and cell cycle regulation were detected at 1 h and 24 h after exposure. Cytotoxic activity was assayed at 1 h after exposure, while perforin and NKG2 D expression were detected at 1 h, 6 h, and 12 h after exposure. To clarify the mechanisms, phosphorylated ERK(p-ERK) was detected at 1 h after exposure. Moreover, microwave-induced cellular apoptosis and cell cycle regulation were analyzed after blockade of ERK signaling by using U0126. Results Microwave-induced morphological and ultrastructural injury, dose-dependent apoptosis(P < 0.001) and cell cycle arrest(P < 0.001) were detected at 1 h after microwave exposure. Moreover, significant apoptosis was still detected at 24 h after 50 m W/cm^2 microwave exposure(P < 0.01). In the 30 m W/cm^2 microwave exposure model, microwaves impaired the cytotoxic activity of NK-92 cells at 1 h and down regulated perforin protein both at 1 h and 6 h after exposure(P < 0.05). Furthermore, p-ERK was down regulated at 1 h after exposure(P < 0.05), while ERK blockade significantly promoted microwave-induced apoptosis(P < 0.05) and downregulation of perforin(P < 0.01). Conclusion Microwave dose-dependently induced morphological and functional injury in NK-92 cells, possibly through ERK-mediated regulation of apoptosis and perforin expression. | ZHAO Li LI Jing HAO Yan Hui GAO Ya Bing WANG Shui Ming ZHANG Jing DONG Ji ZHOU Hong Mei LIU Shu Chen PENG Rui Yun | 2017 | Biomedical and Environmental Sciences2017，30，5： | 5 |
| 3 | Fast-Suppressor Screening for New Components in Protein Trafficking,Organelle Biogenesis and Silencing Pathway in Arabidopsis thaliana Using DEX-Inducible FREE1-RNAi Plants显示文摘Membrane trafficking is essential for plant growth and responses to external signals.The plant unique FYVE domain-containing protein FREE1 is a component of the ESCRT complex(endosomal sorting complex required for transport).FREE1 plays multiple roles in regulating protein trafficking and organelle biogenesis including the formation of intraluminal vesicles of multivesicular body(MVB),vacuolar protein transport and vacuole biogenesis,and autophagic degradation.FREE1 knockout plants show defective MVB formation,abnormal vacuolar transport,fragmented vacuoles,accumulated autophagosomes,and seedling lethality.To further uncover the underlying mechanisms of FREE1 function in plants,we performed a forward genetic screen for mutants that suppressed the seedling lethal phenotype of FREE1-RNAi transgenic plants.The obtained mutants are termed as suppressors of free1(sof).To date,229 putative sof mutants have been identified.Barely detecting of FREE1 protein with M3plants further identified 84 FREE1-related suppressors.Also145 mutants showing no reduction of FREE1 protein were termed as RNAi-related mutants.Through next-generation sequencing(NGS)of bulked DNA from F2mapping population of two RNAi-related sof mutants,FREE1-RNAi T-DNA inserted on chromosome 1 was identified and the causal mutation of putative sof mutant is being identified similarly.These FREE1-and RNAi-related sof mutants will be useful tools and resources for illustrating the underlying mechanisms of FREE1 function in intracellular trafficking and organelle biogenesis,as well as for uncovering the new components involved in the regulation of silencing pathways in plants. | Qiong Zhao Caiji Gao PoShing Lee Lin Liu Shaofang Li Tangjin Hu Jinbo Shen Shuying Pan Hao Ye Yunru Chen Wenhan Cao Yong Cui Peng Zeng Sheng Yu Yangbin Gao Liang Chen Beixin Mo Xin Liu Shi Xiao Yunde Zhao Silin Zhong Xuemei Chen Liwen Jiang | 2015 | Journal of Genetics and Genomics2015，42，6： | 4 |
| 4 | Exploring the Bridge Cases’Role in the Transmission of the SARSCoV-2 Delta Variant—Ruili City,Yunnan Province,China,July–September 2021显示文摘Summary What is already known about this topic?The severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)Delta variant has proved to have increased transmissibility,and mutations that can cause partial immune escape,which makes its transmission more insidious.What is added by this report?This study showed that probable cases who had negative results in nucleic acid testing but had positive IgM test result and/or IgG test value of over 20 S/CO in antibodies testing. | Xiangyu Yan Litao Chang Zekun Wang Linhui Hao Zhongwei Jia Bo Zhang Tiejun Shui | 2021 | China CDC weekly2021，3，50： | 4 |
| 5 | Interactive effect of shade and PEG-induced osmotic stress on physiological responses of soybean seedlings显示文摘Intensively farmed crops used to experience numerous environmental stresses.Among these,shade and drought significantly influence the morpho-physiological and biochemical attributes of plants.However,the interactive effect of shade and drought on the growth and development of soybean under dense cropping systems has not been reported yet.This study investigated the interactive effect of PEG-induced osmotic stress and shade on soybean seedlings.The soybean cultivar viz.,C-103 was subjected to PEG-induced osmotic stress from polyethylene glycol 6000(PEG-6000)under shading and non-shading conditions.PEG-induced osmotic stress significantly reduced the relative water contents,morphological parameters,carbohydrates and chlorophyll contents under both light environments.A significant increase was observed in osmoprotectants,reactive oxygen species and antioxidant enzymes in soybean seedlings.Henceforth,the findings revealed that,seedlings grown under non-shading conditions produced more malondialdehyde and hydrogen peroxide contents as compared to the shade-treated plants when subjected to PEG-induced osmotic stress.Likewise,the shaded plants accumulated more sugars and proline than non-shaded ones under drought stress.Moreover,it was found that nonshaded grown plants were more sensitive to PEG-induced osmotic stress than those exposed to shading conditions,which suggested that shade could boost the protective mechanisms against osmotic stress or at least would not exaggerate the adverse effects of PEG-induced osmotic stress in soybean seedlings. | Muhammad Ahsan ASGHAR JIANG Heng-ke SHUI Zhao-wei CAO Xi-yu HUANG Xi-yu Shakeel IMRAN Bushra AHMAD ZHANG Hao YANG Yue-ning SHANG Jing YANG Hui YU Liang LIU Chun-yan YANG Wen-yu SUN Xin DU Jun-bo | 2021 | Journal of Integrative Agriculture2021，20，9： | 3 |
| 6 | Enhanced antitumor effects of DC-activated CIKs to chemotherapy treatment in a single cohort of advanced non-small-cell lung cancer patients显示文摘 | Lili Yang Baozhu Ren Hui Li Jinpu Yu Shui Cao Xishan Hao Xiubao Ren | 2013 | Cancer Immunology Immunotherapy2013，，1： | 2 |
| 7 | Autologous cytokine-induced killer cell immunotherapy in lung cancer: a phase II clinical study显示文摘 | Runmei Li Changli Wang Liang Liu Chunjuan Du Shui Cao Jinpu Yu Shizhen Emily Wang Xishan Hao Xiubao Ren Hui Li | 2012 | Cancer Immunology Immunotherapy2012，，11： | 2 |
| 8 | CD8 + T Cells That Produce Interleukin-17 Regulate Myeloid-Derived Suppressor Cells and Are Associated With Survival Time of Patients With Gastric Cancer显示文摘 | Yuan Zhuang Liu–Sheng Peng Yong–Liang Zhao Yun Shi Xu–Hu Mao Weisan Chen Ken C. Pang Xiao–Fei Liu Tao Liu Jin–Yu Zhang Hao Zeng Kai–Yun Liu Gang Guo Wen–De Tong Yan Shi Bin Tang Na Li Shu Yu Ping Luo Wei–Jun Zhang Dong–Shui Lu Pei–Wu Yu Quan–Ming Zou | 2012 | Gastroenterology2012，，4： | 2 |
| 9 | Mid-gut volvulus and mesenteric vessel thrombosis in pregnancy:case report and literature review显示文摘 | Shui Losa Hao Rafi Junaid Corder Allan Mowbray David | 2010 | Archives of Gynecology and Obstetrics2010，28，3： | 1 |
| 10 | Extensive Alterations in DNA Methylation and Transcription in Rice Caused by Introgression from Zizania Latifolia显示文摘 | Zhenlan Liu Yongming Wang Ye Shen Wanli Guo Shui Hao Bao Liu | 2004 | Plant Molecular Biology2004，，4： | 1 |
| 11 | Configuration of nucleolar DNA in situ in nucleolus of Allium cepa cells显示文摘The location and configuration of nucleolar DNA have not been determined for a long time. In this paper, we have observed the nucleolar infrastructure and the character of nucleolar DNA in Allium cepa cells by conventional electron microscopy and the cytochemical NAMA-Ur DNA specific staining method. Furthermore, we have properly improved the NAMA-Ur method so as to analyze the location and configuration of nucleolar DNA in situ. Our results indicated that the nucleolar DNA in Allium cepa cells is mainly located at the border between fibrillar centers and dense fibribllar component, especially distributed in the configuration of encircling the fibrillar centers. | TAO Wei HE Jie JIAO Mingda HE Mengyuan HAO Shui | 2001 | Chinese Science Bulletin2001，46，1： | 1 |
| 12 | An agent-based Web service workflow model for inter-enterprise collaboration显示文摘 | Shen Weiming Hao Qi | 2006 | Expert Systems With Applications2006，31，4： | 1 |
| 13 | Inorganic microporous membranes for hydrogen separation:Challenges and solutions显示文摘Porous membrane separation is a competitive hydrogen purification technology due to the advantages of environmental friendliness,energy-saving,simple operation,and low cost.Benefiting from the booming development of materials science and chemical science,great progress has been made in H_(2) separation with porous membranes.This review focuses on the latest advances in the design and fabrication of H_(2) separation inorganic microporous membranes,with emphasis on the synthetic strategies to achieve structural integrity,continuity and stability.This review starts with a brief introduction to the membrane separation mechanisms,followed by an elaboration on the synthetic challenges and corresponding solutions of various high-performance inorganic microporous membranes based on zeolites,silica,carbon,and metal-organic frameworks(MOFs).At last,by highlighting the prospects of ultrathin two-dimensional(2D)porous membranes,we wish to shed some light on the further development of new materials and membranes for highly efficient hydrogen separation. | Ahui Hao Xin Wan Xiaofang Liu Ronghai Yu Jianglan Shui | 2022 | Nano Research Energy2022，1，2： | 1 |
| 14 | An agent-based Web service workflow model for inter-enterprise collaboration 显示文摘 | Wang Shuying Shen Weiming Hao Qi | 2006 | Expert systems with applications2006，31，4： | 1 |
| 15 | Extensive Alterations in DNA Methylation and Transcription in Rice Caused by Introgression from Zizania Latifolia显示文摘 | Zhenlan Liu Yongming Wang Ye Shen Wanli Guo Shui Hao Bao Liu | 2004 | Plant Molecular Biology2004，，4： | 1 |
| 16 | Hexabromocyclododecane-induced Genotoxicity in Cultured Human Breast Cells through DNA Damage显示文摘调查 genotoxicity 并且揭示 Hexabromocyclododecane (HBCD ) 的潜在的毒物学的机制,人的胸房间 HBL-100 为 24 h 暴露于 HBCD 集中(0, 5, 10,和 50 mg/L ) 的一个序列。与一系列酶学和分子的生物学方法,我们发现 HBCD 导致了剂量依赖者 HBL-100 DNA 上的氧化应力。是在 HBCD 的更低的集中在 qRT-PCR,激活的预示的因素 ATM 下面调整的肿瘤 suppressor 基因 BRCA1 和推动的 DNA 修理基因 hOGG1 和 hMTH1 表示揭示了(< 10 mg/L ) 。然而, DNA 修理被 HBCD (50 mg/L ) 的更高的集中象房间增长率一样禁止。推断的结果 HBCD 的 genotoxicity 是剂量依赖者并且与 DNA 修理有关小径。 | LI Rui Jing GAO Hui NA Guang Shui LU Zi Hao YAO Yao YANG Fan | 2017 | Biomedical and Environmental Sciences2017，30，4： | 1 |
| 17 | Differentiation of various kinds of Fructus schisandrae by surface desorption atmospheric pressure chemical ionization mass spectrometry combined with Principal Component Analysis显示文摘 | Zifeng Pi Hao Yue Li Ma Liying Ding Zhiqiang Liu Shuying Liu | 2011 | Analytica Chimica Acta2011，，2： | 1 |
| 18 | An agent-based Web service workflow model forinter-enterprise collaboration显示文摘 | Weiming Shen Qi Hao | 2006 | Expert Systems wi th Applications2006，31，4： | 1 |
| 19 | A comparative study of sputtered TaCx and WCx films as diffusion barriers between Cu and Si显示文摘 | Wang Shui Jinn Tsai Hao Yi Sun S C | 2001 | Thin Solid Films2001，394，： | 1 |
| 20 | An agent- based Web service workflow model for inter- enterprise collaboration显示文摘 | Wang Shuying Shen Weiming Hao Qi | 2006 | Expert Systems with Applications2006，31，4： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员