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| 1 | Tobacco RING E3 Ligase NtRFP1 Mediates Ubiquitination and Proteasomal Degradation of a Geminivirus-Encoded βC1显示文摘西红柿黄叶卷屈中国联系病毒的 betasatellite 编码的 C1 蛋白质作为一个致病力决定因素工作。更好理解 C1 在致病力工作的分子的基础,酵母一个烟草 cDNA 图书馆的二混血儿的屏幕作为诱饵用 C1 被执行。屏幕表明 C1 与烟草戒指手指蛋白质交往指明了 NtRFP1,它被双分子的荧光互补和 co-immunoprecipitation 试金进一步在 Nicotiana benthamiana 房间证实。NtRFP1 的表示被 C1 导致,并且在 vitro ubiquitination,试金证明 NtRFP1 是调停的功能的 E3 ubiquitin ligase C1 ubiquitination。另外, C1 被显示是在 vivo 的 ubiquitinated 并且由植物 26S proteasome 降级。在病毒的感染以后,植物 overexpressing NtRFP1 开发了稀释症状,而有 NtRFP1 的 silenced 表示的植物显示出严重症状。证据的另外的线证明 NtRFP1 通过由 26S proteasome 支持它的降级稀释导致 C1 的症状。一起拿,我们的结果建议烟草戒指 E3 ligase NtRFP1 由与 C1 交往调停稀释疾病症状它经由 ubiquitin/26S proteasome 系统的 ubiquitination 和降级。 | Qingtang Shen Tao Hu Min Bao Linge Cao Huawei Zhang Fengmin Song Qi Xie Xueping Zhou | 2016 | Molecular Plant2016，9，6： | 8 |
| 2 | Regioselective oxidation of tetracycline by permanganate through alternating susceptible moiety and increasing electron donating ability显示文摘Permanganate has attracted much attention in wide range of chemistry and particularly in degradation of environmental pollutants.However,few studies have discussed the feature of regioselective reactivity of permanganate with specific moiety of the target compound.Herein,we studied the reaction between permanganate and tetracycline that is an emerging micropollutant with different species containing several electron-rich groups.The second-order rate constants increased from 6.0 to 9.0 and could be quantitatively modeled by considering the speciation of both reactants,yielding kTC0=11.7(mol/L)^-1 sec^-1,kTC-=35.7(mol/L)^-1 sec^-1,kTC2-=43.1(mol/L)^-1 sec^-1 for individual reaction channels.Degradation products were then identified as the hydroxylated and demethylated compounds.The result suggested a rate-limiting step of simple hydroxylation at the phenolic and/or alkene moieties,while the demethylation should be caused by the unavoidably formed manganese oxide via single electron oxidation.This is supported by the DFT calculation,indicating the primary oxidation of phenolic group of TC0 with activation barrier of 44.5 kcal/mol and of alkene group of TC-and TC2-with activation barriers of 44.0 and 43.4 kcal/mol,respectively.This is in agreement with the experimental results,implying the alternation of regioselectivity associated with the deprotonation process.The result was further supported by performing the Fukui function and electrostatic potential analysis,reflecting the more probable site and better electron donating tendency beneficial to the permanganate oxidation. | Xiaohua Jiang William AJefferson Dean Song Hanyang Cheng Fengmin Li Zhimin Qiang Aiqian Zhang Huijuan Liu Jiuhui Qu | 2020 | Journal of Environmental Sciences2020，32，1： | 1 |
| 3 | Diagnostic value of anti-cyclic citrullinated peptide antibodies in northern Chinese Han patients with rheumatoid arthritis and its correlation with disease activity显示文摘 | Li Hui Song Wuqi Li Yang Liu Yanhong Bai Jing Li Xiu Mu Fengyun Wang Yao Zhang Fengshan Su Liju Zhang Fengmin | 2010 | Clinical Rheumatology2010，，4： | 1 |
| 4 | How two ridges and the furrow mulched with plastic film affect soil water, soil temperature and yield of maize on the semiarid Loess Plateau of China显示文摘 | Zhou Limin Li Fengmin Jin Shengli Song Yajie | 2009 | Field Crops Research2009，113，1： | 1 |
| 5 | Effects of pre-sowing irrigation and phosphorus application on wa- ter use and yield of spring wheat under semi-arid condi- tions显示文摘 | LI Fengmin SONG Qiuhua LIU Hongsheng | 2001 | Agric Water Manage2001，49，3： | 1 |
| 6 | Assessing heavy metal pollution and potential ecological risk of tea plantation soils显示文摘Accumulation of heavy metals in agricultural soils tends to increase crop uptake of heavy metals,and can adversely impact human health through food chains.This study assessed the heavy metal pollution status and the potential ecological risk of tea plantation soils in southern Shaanxi Province,China.A total of 330 topsoil samples were collected to analyze the concentrations of seven heavy metals(Pb,Zn,Cu,Cr,Hg,As and Cd)and the pH level.The Hakanson potential ecological risk index was used based on the background levels of soil heavy metals in Shaanxi.Results showed that the averaged concentrations of soil Pb,Zn,Cu,Cr,Hg,As,and Cd were 10.0,87.6,16.4,12.4,0.2,6.9 and 0.1 mg/kg,respectively.Cd and Hg concentrations exceeded level II of China’s Soil Environment Quality Standard(GB 15618-2009)in 9.3%and 7.0%of the total samples,respectively.The coefficient of variation ranged from 29.2%to 52.2%for different elements,and was 49.3%for Cd and 48.6%for Hg.The averaged comprehensive potential ecological risk index was 95.4,and the overall potential ecological risk was low,with 82.4%of the total samples at a low level of potential ecological risk.Cd and Hg contributed most to the potential ecological risk(35.5%and 46.5%,respectively),and the risks associated with other elements were relatively minor.This study suggested that soil Cd and Hg pollution should be controlled to ensure the safe production of tea in the study area. | Zuoping Zhao Sha Yan Min Duan Jing Fu Qi Wang Zhifeng Liu Fengmin Song Bo Tang Chen Li Gongwei Qin Yanan Tong | 2019 | International Journal of Agricultural and Biological Engineering2019，12，6： | 0 |
| 7 | Web Resources for Stem Cell Research显示文摘In this short review, we have presented a brief overview on major web resources relevant to stem cell research. To facilitate more efficient use of these resources, we have provided a preliminary rating based on our own user experience of the overall quality for each resource. We plan to update the information on an annual basis. | Ting Wei Xing Peng Lili Ye Jiajia Wang Fuhai Song Zhouxian Bai Guangchun Han Fengmin Ji Hongxing Lei | 2015 | Genomics, Proteomics & Bioinformatics2015，13，1： | 0 |
| 8 | Enhanced triallyl isocyanurate(TAIC)degradation through application of an 03/UV process:Performance optimization and degradation pathways显示文摘Triallyl isocyanurate(TAIC,C12H15N3O3)has featured in wastewater treatment as a refractory organic compound due to the significant production capability and negative environmental impact.TAIC degradation was enhanced when an ozone(O3)/ultraviolet(UV)process was applied compared with the application of an independent O3 process.Although 99%of TAIC could be degraded in 5 min during both processes,the O3/UV process had a 70%mineralization rate that was much higher than that of the independent O3 process(9%)in 30 min.Four possible degradation pathways were proposed based on the organic compounds of intermediate products identified during TAIC degradation through the application of independent 03 and O3/UV processes.pH impacted both the direct and indirect oxidation processes.Acidic and alkaline conditions preferred direct and indirect reactions respectively,with a pH of 9 achieving maximum Total Organic Carbon(TOC)removal.Both CO32-and HCO3-decreased TOC removal,however only CO32-negatively impacted TAIC degradation.Effects of Cl-as a radical scavenger became more marked only at high concentrations(over 500 mg/L Cl-).Particulate and suspended matter could hinder the transmission of ultraviolet light and reduce the production of HO·accordingly. | Yapeng Song Hui Gong Jianbing Wang Fengmin Chang Kaijun Wang | 2020 | Frontiers of Environmental Science & Engineering2020，14，4： | 0 |
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