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| 1 | miR164c and miR168a regulate seed vigor in rice^∞显示文摘Micro RNAs(miRNAs)are key regulators of gene expression in many important biological processes of plants.However,few miRNAs have been shown to regulate seed vigor.Here,we conducted microarray assays to analyze miRNA expression levels in seeds of the rice(Oryza sativa L.)cultivar ZR02.Results showed significant differences in the expression of 11 miRNAs between artificially aged and untreated control seeds.Among these,osa-miR164c was transcriptionally upregulated,while osamiR168 a was downregulated in artificially aged seeds;this was verified by quantitative real-time PCR analysis.Under the same aging condition,osa-miR164c overexpression in OE164c transgenic seeds and osa-miR168a silencing in MIM168a transgenic seeds of the rice cultivar Kasalath led to lower germination rates,whereas osa-miR164c silencing Rin MIM164c and osa-miR168a overexpression in OE168a resulted in higher seed germination rates compared with wild-type seeds.Meanwhile,changes in cytomembrane permeability of seeds and in the expression level of osa-miR164c target genes(OsPM27 and OsPSK5)and osamiR168a target genes(OsAGO1 and OsPTR2)under aging conditions coincided with changes in seed vigor induced by osa-miR164c and osa-miR168 a.Thus,genetic manipulation of miRNAs has important implications in the development of crop cultivars with high vigor and extended life span of seeds. | Yan Zhou Shiqi Zhou Liping Wang Duo Wu Hailan Cheng Xu Du Dandan Mao Chunlai Zhang Xiaocheng Jiang | 2020 | Journal of Integrative Plant Biology2020，62，4： | 7 |
| 2 | Building a sequence map of the pig pan-genome from multiple de novo assemblies and Hi-C data显示文摘Pigs were domesticated independently in the Near East and China,indicating that a single reference genome from one individual is unable to represent the full spectrum of divergent sequences in pigs worldwide.Therefore,12 de novo pig assemblies from Eurasia were compared in this study to identify the missing sequences from the reference genome.As a result,72.5 Mb of nonredundant sequences(~3% of the genome)were found to be absent from the reference genome(Sscrofa11.1)and were defined as pan-sequences.Of the pan-sequences,9.0 Mb were dominant in Chinese pigs,in contrast with their low frequency in European pigs.One sequence dominant in Chinese pigs contained the complete genic region of the tazarotene-induced gene 3(TIG3)gene which is involved in fatty acid metabolism.Using flanking sequences and Hi-C based methods,27.7% of the sequences could be anchored to the reference genome.The supplementation of these sequences could contribute to the accurate interpretation of the 3D chromatin structure.A web-based pan-genome database was further provided to serve as a primary resource for exploration of genetic diversity and promote pig breeding and biomedical research. | Xiaomeng Tian Ran Li Weiwei Fu Yan Li Xihong Wang Ming Li Duo Du Qianzi Tang Yudong Cai Yiming Long Yue Zhao Mingzhou Li Yu Jiang | 2020 | Science China(Life Sciences)2020，63，5： | 7 |
| 3 | Substantial transition to clean household energy mix in rural China显示文摘The household energy mix has significant impacts on human health and climate,as it contributes greatly to many health-and climate-relevant air pollutants.Compared to the well-established urban energy statistical system,the rural household energy statistical system is incomplete and is often associated with high biases.Via a nationwide investigation,this study revealed high contributions to energy supply from coal and biomass fuels in the rural household energy sector,while electricity comprised∼20%.Stacking(the use of multiple sources of energy)is significant,and the average number of energy types was 2.8 per household.Compared to 2012,the consumption of biomass and coals in 2017 decreased by 45%and 12%,respectively,while the gas consumption amount increased by 204%.Increased gas and decreased coal consumptions were mainly in cooking,while decreased biomass was in both cooking(41%)and heating(59%).The time-sharing fraction of electricity and gases(E&G)for daily cooking grew,reaching 69%in 2017,but for space heating,traditional solid fuels were still dominant,with the national average shared fraction of E&G being only 20%.The non-uniform spatial distribution and the non-linear increase in the fraction of E&G indicated challenges to achieving universal access to modern cooking energy by 2030,particularly in less-developed rural and mountainous areas.In some non-typical heating zones,the increased share of E&G for heating was significant and largely driven by income growth,but in typical heating zones,the time-sharing fraction was<5%and was not significantly increased,except in areas with policy intervention.The intervention policy not only led to dramatic increases in the clean energy fraction for heating but also accelerated the clean cooking transition.Higher income,higher education,younger age,less energy/stove stacking and smaller family size positively impacted the clean energy transition. | Guofeng Shen Rui Xiong Yanlin Tian Zhihan Luo Bahabaike Jiangtulu Wenjun Meng Wei Du Jing Meng Yuanchen Chen Bing Xue Bin Wang Yonghong Duan Jia Duo Fenggui Fan Lei Huang Tianzhen Ju Fenggui Liu Shunxin Li Xianli Liu Yungui Li Mu Wang Ying Nan Bo Pan Yanfang Pan Lizhi Wang Eddy Zeng Chao Zhan Yilin Chen Huizhong Shen Hefa Cheng Shu Tao | 2022 | National Science Review2022，9，7： | 4 |
| 4 | Mitochondrial malic enzyme 2 promotes breast cancer metastasis via stabilizing HIF-1α under hypoxia显示文摘Objective: α-ketoglutarate(α-KG) is the substrate to hydroxylate collagen and hypoxia-inducible factor-1α(HIF-1α), which are important for cancer metastasis. Previous studies have shown that the upregulation of collagen prolyl 4-hydroxylase in breast cancer cells stabilizes the expression of HIF-1α by depleting α-KG levels. We hypothesized that mitochondrial malic enzyme 2(ME2) might also affect HIF-1α expression via modulating α-KG levels in breast cancer cells.Methods: We evaluated ME2 protein expression in 100 breast cancer patients using immunohistochemistry and correlated with clinicopathological indicators. The effect of ME2 knockout on cancer metastasis was evaluated using an orthotopic breast cancer model. The effect of ME2 knockout or knockdown on the levels of α-KG and HIF-1α proteins in breast cancer cell lines was determined both in vitro and in vivo.Results: ME2 was found to be upregulated in the human breast cancerous tissues compared with the matched precancerous tissues(P<0.001). The elevated expression of ME2 was associated with a poor prognosis(P=0.019).ME2 upregulation was also related to lymph node metastasis(P=0.016), pathological staging(P=0.033), and vascular cancer embolus(P=0.014). Also, ME2 knockout significantly inhibited lung metastasis in vivo. In the tumors formed by ME2 knockout cells, the levels of α-KG were significantly increased and collagen hydroxylation level did not change significantly but HIF-1α protein expression was significantly decreased, compared to the control samples. In cell culture, cells with ME2 knockout or knockdown demonstrated significantly higher α-KG levels but significantly lower HIF-1α protein expression than control cells under hypoxia. Exogenous malate and α-KG exerted similar effect on HIF-1α in breast cancer cells to ME2 knockout or knockdown. Additionally,treatment with malate significantly decreased 4 T1 breast cancer lung metastasis. ME2 expression was associated with HIF-1α levels in human breast cancer samples(P=0.008).Conclusions: Our results provide evidence that upregulation of ME2 is associated with a poor prognosis of breast cancer patients and propose a mechanistic understanding of a link between ME2 and breast cancer metastasis. | Duo You Danfeng Du Xueke Zhao Xinmin Li Minfeng Ying Xun Hu | 2021 | Chinese Journal of Cancer Research2021，33，3： | 3 |
| 5 | Frequent RNF43 mutation contributes to moderate activation of Wnt signaling in colorectal signet-ring cell carcinoma显示文摘Dear Editor,Signet-ring cell carcinoma(SRCC)is a rare subtype of colorectal cancer(CRC)characterized histologically by the accumulation of mucins in the cytoplasm and displacement of nuclei to the cellular periphery,accounting for about 1%CRC(Fig.S1A)(Borger et al.,2007).Compare to common subtypes of CRC,such as adenocarcinoma(AC)and mucinous adenocarcinoma(MAC),SRCC is associated with aggressive behaviors and younger age at presentation(Kang et al.,2005;Sung et al.,2008;Nitsche et al.,2013;Hugen et al.,2014;Inamura et al.,2015).A retrospective analysis of CRC patient's data at Fudan University Shanghai Cancer Center(FUSCC)also indicated a worse overall and disease-free survival of SRCC patients(Fig.S1B and S1C,Table S1). | Yaqi Li Jian Li Renjie Wang Long Zhang Guoxiang Fu Xueying Wang Yebin Wang Chuantao Fang Dandan Zhang Duo Du Xiaoji Ma Mengxue Pan Qiang Guo Xiaoya Xu Xiang Hu Yi Zhou Shaobo Mo Huijun Wang Jianjun Gao Shenglin Huang Yun Liu Sanjun Cai Guoqiang Hua Junjie Peng Fa-Xing Yu | 2020 | Protein & Cell2020，11，4： | 2 |
| 6 | An engineering method of determining the delamination fracture toughness of composite laminates显示文摘 | Yan X Q Du S Y Duo W | 1991 | Engineering Fracture Me- chanics1991，39，4： | 1 |
| 7 | A Hu sheep genome with the first ovine Y chromosome reveal introgression history after sheep domestication显示文摘The Y chromosome plays key roles in male fertility and reflects the evolutionary history of paternal lineages.Here,we present a de novo genome assembly of the Hu sheep with the first draft assembly of ovine Y chromosome(o MSY),using nanopore sequencing and Hi-C technologies.The o MSY that we generated spans 10.6 Mb from which 775 Y-SNPs were identified by applying a large panel of whole genome sequences from worldwide sheep and wild Iranian mouflons.Three major paternal lineages(HY1a,HY1b and HY2)were defined across domestic sheep,of which HY2 was newly detected.Surprisingly,HY2 forms a monophyletic clade with the Iranian mouflons and is highly divergent from both HY1a and HY1b.Demographic analysis of Y chromosomes,mitochondrial and nuclear genomes confirmed that HY2 and the maternal counterpart of lineage C represented a distinct wild mouflon population in Iran that diverge from the direct ancestor of domestic sheep,the wild mouflons in Southeastern Anatolia.Our results suggest that wild Iranian mouflons had introgressed into domestic sheep and thereby introduced this Iranian mouflon specific lineage carrying HY2 to both East Asian and Africa sheep populations. | Ran Li Peng Yang Ming Li Wenwen Fang Xiangpeng Yue Hojjat Asadollahpour Nanaei Shangquan Gan Duo Du Yudong Cai Xuelei Dai Qimeng Yang Chunna Cao Weidong Deng Sangang He Wenrong Li Runlin Ma Mingjun Liu Yu Jiang | 2021 | Science China(Life Sciences)2021，64，7： | 1 |
| 8 | GNSS techniques for real-time monitoring of landslides:a review显示文摘Currently,Global Navigation Satellite System(GNSS)Real-Time Kinematic positioning(RTK)and Precise Point Positioning(PPP)techniques are widely employed for real-time monitoring of landslides.However,both RTK and PPP monitoring techniques have their limitations,such as limited service coverage or long convergence times.PPP-RTK technique which integrates RTK and PPP is a novel approach for monitoring landslides with the advantages of rapid convergence,high-precision,and a wide service area.This study summarizes the limitations of RTK,PPP,and PPP-RTK monitoring techniques and suggests some improved strategies.Their performances are compared and analyzed using real monitoring data.The experiment results demonstrate that RTK is the best option for small-scale(the baseline distance<15 km)and real-time landslide monitoring without considering the cost.PPP technique converges to centimeter-level accuracy in tens of minutes,only suitable for the stability analysis of reference stations.Over a large area(the baseline distance<100 km),PPP-RTK can provide excellent horizontal accuracy and adapt the service range in response to the demand for monitoring accuracy,as the vertical accuracy is signifcantly impacted by the service range and elevation diference.Finally,the characteristics of three techniques are integrated to form a comprehensive landslide monitoring technique that considers intelligence,robustness,and real-time. | Guanwen Huang Shi Du Duo Wang | 2023 | Satellite Navigation2023，4，1： | 1 |
| 9 | Preparation and characterization of TiO2 nanopaticles/CNTs composite particles显示文摘 | Wang mingqiu Yan jun Du shi duo | 2013 | Appl Mechan Mater2013，，： | 1 |
| 10 | Efficient Transposition of the Retrotransposon Tnt1 in Cucumber(Cucumis sativus L.)显示文摘Tnt1 is an active retrotransposon originally identified in tobacco(Nicotiana tabacum L.)(Grandbastien et al.,1989),but its transposition activity could be activated through tissue culture in other plant species.The insertions are stable and inheritable in the progeny,which has made it a valuable and versatile tool for developing insertional mutagenesis libraries in several plant species.Here,we explored its utility for mutagenesis in cucumber(Cucumis sativus L.).T_3 Tnt1 transgenic cucumber plants were subjected to tissue culture to regenerate self-pollinated progeny.With PCR and analyses and Southern hybridization,we found regenerated plants maintained the original Tnt1 insertion and created new insertions suggesting characteristic re-transposition activity of Tnt1 during this process.Using genome walking,some flanking sequences of Tnt1 insertions were recovered in regenerated plants.The results demonstrated that Tnt1 could be stably inherited and re-transposable during tissue culture in cucumber and that it is feasible to use for developing an insertional mutagenesis library for cucumber. | ZHANG Qi DU Hui LV Duo XIAO Tingting PAN Jian HE Huanle WANG Gang CAI Run WENG Yiqun PAN Junsong | 2018 | Horticultural Plant Journal2018，4，3： | 0 |
| 11 | Stability analysis of reference station and compensation for monitoring stations in GNSS landslide monitoring显示文摘The Real-Time Kinematic(RTK)positioning method of the Global Navigation Satellite System(GNSS)has been widely used for landslide monitoring.The stability of its reference station is crucial to obtain accurate and reliable monitoring results.Unstable reference stations due to the geological environment and human activities are difficult to detect and in practical applications often ignored.As a result,it affects the positioning solutions and subsequently the interpretation and detection of landslide motions,which must be addressed in GNSS landslide monitoring.To solve this problem,we propose using the Precise Point Positioning(PPP)technique to analyze the stability of the reference station by verifying its position.The deformations of the monitoring stations are then compensated.First,the reference station coordinates are obtained by the PPP technique and tectonic motion is considered in data processing.The change or breakout of the reference station position is then determined using a cumulative sum control chart method.Finally,each monitoring station’s displacements are compensated according to the displacements of the reference station.According to the results of the Tengqing landslide experiment,the PPP technique can be used in GNSS landslide monitoring to analyze the stability of reference stations.With PPP,millimeter-level accuracy for the coordinates of reference stations is achieved.Compared to the traditional deformation series,the compensated displacement series more reliably reflects the landslide motions.This study will increase the reliability of monitoring results and contribute to implementing GNSS in monitoring landslides. | Duo Wang Guanwen Huang Yuan Du Qin Zhang Zhengwei Bai Jing Tian | 2023 | Satellite Navigation2023，4，3： | 0 |
| 12 | Recent Advances in Fibrous Materials for Interfacial Solar Steam Generation显示文摘The scarcity of fresh water resources has become a serious issue hindering the sustainable development of modern civilization.The interfacial solar steam generation(ISSG)system that produces heat on material surface through photothermal conversion for desalination has been demonstrated as a promising candidate for practical application.Fibrous materials with unique flexibility,durability,processability,practicability,and multifunctionality have attracted considerable attention in the ISSG field.In this review,the basics of fibrous materials,such as their classification,manufacturing methods and flexible fibrous structure,are firstly introduced.Afterward,the outstanding properties of fibrous materials on different dimensions are demonstrated,as well as the versatile morphologies and structures that allow fibrous materials to carry out different roles in ISSG.Moreover,the practicability and multifunctionality of fibrous materials are illustrated in detail by combining specific cases to show their promising potential in practical ISSG application.Finally,existing challenges and future opportunities of fibrous material-based ISSG systems are discussed. | Can Ge Duo Xu Heng Du Ze Chen Jingyu Chen Zhuoer Shen Weilin Xu Qian Zhang Jian Fang | 2023 | Advanced Fiber Materials2023，5，3： | 0 |
| 13 | Polymodal Functionality of C. elegans OLL Neurons in Mechanosensation and Thermosensation显示文摘Sensory modalities are important for survival but the molecular mechanisms remain challenging due to the polymodal functionality of sensory neurons. Here, we report the C. elegans outer labial lateral(OLL) sensilla sensory neurons respond to touch and cold. Mechanosensation of OLL neurons resulted in cell-autonomous mechanically-evoked Ca~(2+) transients and rapidly-adapting mechanoreceptor currents with a very short latency.Mechanotransduction of OLL neurons might be carried by a novel Na~+ conductance channel, which is insensitive to amiloride. The bona fide mechano-gated Na~+ -selective degenerin/epithelial Na~+ channels, TRP-4, TMC, and Piezo proteins are not involved in this mechanosensation.Interestingly, OLL neurons also mediated cold but not warm responses in a cell-autonomous manner. We further showed that the cold response of OLL neurons is not mediated by the cold receptor TRPA-1 or the temperaturesensitive glutamate receptor GLR-3. Thus, we propose the polymodal functionality of OLL neurons in mechanosensation and cold sensation. | Yuedan Fan Wenjuan Zou Jia Liu Umar Al-Sheikh Hankui Cheng Duo Duan Du Chen Siyan Liu Luyi Chen Jilei Xu Firdosh Ruhomutally Lijun Kang | 2021 | Neuroscience Bulletin2021，37，5： | 0 |
| 14 | A photopatterned SERS substrate with a sandwich structure for multiplex detection显示文摘Substrate photopatterning has provided versatile applications in biomedical fields. Herein, an universal and efficient photoligation reaction has been used to prepare a patterned capture substrate for a sandwich SERS immunoassay. Photoirradiation induces mild and efficient immobilization of antibodies at the desired region of a gold surface, and the antibody-antigen interaction helps the substrate to capture the antigens in solution specifically. After exposing to SERS probes, i.e., the gold nanoparticles labelled with both antibodies and intrinsically strong Raman reporters, multiple quantitative SERS determination of antigens can be achieved with high sensitivity and specificity. The limit of detection can be as low as 10^(-12) mol/L for four kinds of cancer biomarkers, which provides a promising method for the construction of highly sensitive and high-throughput SERS detection chip and the application of in vitro diagnosis. | Yuan Xue Duo Liu Xuebin Wang Yanxin Xiang Shengjie Du Kai Ye Chunyan Bao Linyong Zhu | 2022 | Chinese Chemical Letters2022，33，3： | 0 |
| 15 | 建立具有体内胚内和胚外发育潜能的多能干细胞显示文摘文章简介在已知的体外培养的干细胞类型当中,多能干细胞具有最高的发育潜能,能够形成构成成年个体的所有细胞类型。但是,多能干细胞很难在体内发育过程中形成胚外的胎盘组织。 | Yang Yang Bei Liu Jun Xu Jinlin Wang Jun Wu Cheng Shi Yaxing Xu Jiebin Dong Chengyan Wang Weifeng Lai Jialiang Zhu Liang Xiong Dicong Zhu Xiang Li Weifeng Yang Takayoshi Yamauchi Atsushi Sugawara Zhongwei Li Fangyuan Sun Xiangyun Li Chen Li Aibin He Yaqin Du Ting Wang Chaoran Zhao Haibo Li Xiaochun Chi Hongquan Zhang Yifang Liu Cheng Li Shuguang Duo Ming Yin 沈浣 Juan Carlos Izpisua Belmonte 邓宏魁 | 2018 | 科学新闻2018，0，4： | 0 |
| 16 | Sex differences in eye movements and neural oscillations during mental rotation in virtual reality显示文摘Virtual reality(VR)has been a promising tool for developing visuospatial tasks.Among visuospatial tasks,mental rotation tasks are widely used in the assessment of visuospatial ability.Males have a distinct advantage in mental rotation ability compared to females,yet it is generally produced by investigations based on two-dimensional(2D)images on a computer screen.Sex differences in mental rotation tasks with three-dimensional(3D)objects in VR were not fully investigated.It is unclear whether the male's advantages in 2D mental rotation tasks are weakened in 3D tasks.The aim of this study was to provide new insights into the understanding of sex differences in mental rotation tasks presented in VR.Here,we developed a VR mental rotation task(VR-MRT)using 3D objects presented by a head-mounted display(HMD)and used VR-based eye tracking and electroencephalography(EEG)to examine eye movements and neural oscillations for males and females.Our results showed that females preferred a piecemeal strategy compared to males,suggesting a significant sex difference in visual strategy.More importantly,we found no significant sex differences in alpha-band and beta-band oscillations related to rotation processes of VR-MRT.These findings indicated that sex differences in the VR-MRT were mainly attributed to the selection of visual strategy rather than the rotation processes.The study helps to comprehensively understand the dominant factors contributing to the sex differences in the VR-MRT. | Zhili Tang Xiaoyu Liu Hongqiang Huo Min Tang Xiaofeng Qiao Duo Chen Ying Dong Linyuan Fan Jinghui Wang Xin Du Jieyi Guo Yubo Fan | 2023 | Medicine in Novel Technology and Devices2023，，2： | 0 |
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