|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Selection and development of representative simple sequence repeat primers and multiplex SSR sets for high throughput automated genotyping in maize显示文摘In the current study, 1900 maize simple sequence repeat (SSR) primers published in MaizeGDB were screened utilizing reference literature, 15 representative Chinese maize inbred lines and 15 Chinese maize hybrids from national regional testing. In total, 500 highly polymorphic primers were identified and used to construct a genetic map. 100 evenly distributed primers, 10 primers per chromosome, were further selected as a set of universal SSR core primers, recommended as preferred primers for general studies. These core primers were then redesigned and used to construct a high throughput multiplex PCR system based on a five-color fluorescence capillary detection system. We report here that two sets of ten-plex PCR combinations have been constructed, each consisting of 10 primers, with one primer per chromosome. | WANG FengGe ZHAO JiuRan DAI JingRui YI HongMei KUANG Meng SUN YanMei YU XinYan GUO JingLun WANG Lu | 2007 | Chinese Science Bulletin2007，52，2： | 35 |
| 2 | Relationship between differ- ential gene expression pat- terns in functional leaves of maize (Zea mays L.) at milk filling stage and heterosis using cDNA-AFLP显示文摘To understand the molecular mechanism of maize heterosis, differential gene expression patterns in the functional leaves of 35 maize hybrids relative to their parents involving 10 elite inbreds at milk filling stage were analyzed by using cDNA-AFLP. The correlation analyses of various differential expression patterns with the performance and heterosis of main maize agronomic traits were evaluated. The main results were as follows: For uniparental specific expression, significant positive correlations were detected with the performance of seed weight per ear and 100-seed weight at 0.01 and 0.05 probability levels respectively. For biparental specific expression, significant negative correla-tions were detected with the performance of ear diameter and seed weight per ear at 0.01 probability level. For uni-parental specific expression, significant positive correlations were detected with the heterosis of ear diameter and seed weight per ear at 0.01 and 0.05 probability levels respectively. For biparental specific expression, significant negative cor-relation was detected with the heterosis of ear diameter at 0.05 probability level. However, for F1-specific expression, for fragments detected only in one parent and F1, and for fragments detected only in two parents or only in F1, no sig-nificant correlation was detected with the performance or heterosis of all agronomic traits surveyed. | TIAN Zengyuan & DAI Jingrui National Maize Improvement Center of China, China Agricultural Uni-versity, Beijing 100094, China Correspondence should be addressed to Tian Zengyuan (e-mail: tian-zengyuan@yahoo.com.cn) | 2003 | Chinese Science Bulletin2003，48，1： | 10 |
| 3 | Construction of a genetic map and location of quantitative trait loci for dwarf trait in maize by RFLP markers显示文摘Using F2 population derived from the cross of tall inbred 7922 by dwarf inbred 5003, an RFLP linkage map of maize has been constructed, on which 85 markers are distributed among 10 linkage groups and span maize genome about 1827.8 cM with an average distance (24.4 cM) between markers. 106 F2:3 lines of the population were grown in a 10×11 simple rectangular lattice design of one-raw plots with two replications and evaluated for plant height (PH). With interval mapping procedure, 5 QTLs controlling plant height have been identified and their genetic effects and gene action determined. 2 major OTLs with opposite effect have been discovered. One for increasing plant height is ph 1 which is located at chromosome 2 and accounts for 51.8% of the total phenotypic variation; the other for decreasing plant height is ph3 which is located at chromosome 5 and accounts for 38.6% of the total phenotypic variation. The chromosomal location of ph3 might be the same as or close to the position of bv1, a dwarf mutant | CAO Yongguo WANG Guoying WANG Shoucai WEI Yanling LU Jiang XIE Youju DAI Jingrui | 2000 | Chinese Science Bulletin2000，45，3： | 9 |
| 4 | Cloning and characterization of a novel R1-MYB transcription factor in maize显示文摘R1-MYB transcription regulatory factors play vital roles in transcriptional modification during higher plant metabolism and development. In the present study, an R1-MYB gene was isolated from the maize (Zea mays L.) inbred line C8605-2 based on expressed sequence tags (ESTs), which expressed differentially between the hybrid C8605-2 × W1445 and its two parents in an oligonucleotide microarray. The full-length cDNA, designated as ZmMYBE1 (GeneBank Accession No. FJ024049), consists of 1992 nucleotides and contains a 1296-bp open reading frame. One conserved MYB domain was detected near the N-terminus of the deduced amino acid sequence, where an acidic Ser/Thr-rich area was also observed in the downstream region. The sequence of ZmMYBE1, mapped to chromosome 5 (bin 5.03), was revealed belonging to a multi-gene family in the maize genome. Subcellular localization analysis determined that ZmMYBE1 was located in the nucleus. Expression analysis showed that ZmMYBE1 transcripts accumulated in various tissues examined, with high expression levels in the immature ear and low levels in the tassel, root, and stem. The expression analysis for ZmMYBE1 in immature ears showed the highest expression level at the earliest vegetative developmental stage of the plant. Expression of ZmMYBE1 higher than that of the parents was observed in the hybrid C8605-2 × W1445. And also the expression level similar to the lowest of the parents in the hybrid C8605-2 × W245 was observed, revealing different expression patterns for the same gene in various hybrids combined from diversified inbred lines. Transgenic Arabidopsis overexpressing ZmMYBE1 implies that ZmMYBE1 has an important role in developmental regulation in maize. | Guanqing Jia, Bo Li, Dengfeng Zhang, Tifu Zhang, Zhiyong Li, Jingrui Dai, Shoucai Wang* National Maize Improvement Center of China, Key Laboratory of Crop Genomics and Genetic Improvement of Agriculture Ministry, Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China | 2009 | Progress in Natural Science:Materials International2009，19，9： | 6 |
| 5 | Detection of quantitative trait loci and heterotic loci for plant height using an immortalized F_2 population in maize显示文摘A set of recombinant inbred lines (RIL) derived from Yuyu22, an elite hybrid widespread in China, was used to construct an immortalized F2 (IF2) population comprising 441 different crosses. Genetic linkage maps were constructed containing 10 linkages groups with 263 simple sequence repeat (SSR) molecular markers. Twelve and ten quantitative trait loci (QTL) were detected for plant height in the IF2 and RIL populations respectively, using the composite interval mapping method, and six same QTL were identified in the two populations. In addition, ten unique heterotic loci (HL) located on seven different chromosomes were revealed for plant height using the mid-parent heterosis as the input data. These HL explained 1.26%―8.41% of the genotypic variance in plant height heterosis and most expressed overdominant effects. Only three QTL and HL were located in the same chromosomal region, it implied that plant height and its heterosis might be controlled by two types of genetic mechanisms. | TANG JiHua MA XiQing TENG WenTao YAN JianBing WU WeiRen DAI JingRui LI JianSheng | 2007 | Chinese Science Bulletin2007，52，4： | 5 |
| 6 | cDNA-AFLP analysis reveals that maize resistance to Bipolaris maydis is associated with the induction of multiple defense-related genes显示文摘The fungal pathogen Bipolaris maydis invades by direct penetration into maize leaf veins. In order to understand the resistance mechanism of maize to B. maydis strain 523, cDNA-AFLP (amplified fragment length polymorphism) analysis was conducted to compare the changes in mRNA transcripts hi response to B. maydis infection between a highly disease-resistant (HDR) line and a susceptible (S) line. 13 cDNA fragments derived from the genes showing enhanced expression after fungal infection, named HDR genes, were isolated from the HDR line. Northern blot analysis showed that 5 HDR genes were induced by fungal infection in the HDR, but not the S lines. The 5 HDR genes showed homology to previously characterized genes involved in disease resistance. A full-length HDR10 cDNA was isolated. It had a capacity to encode a protein of 284 amino acids. The deduced amino acid sequence of the HDR10 gene was homologous to a fungal infection-induced protein from Cicer arietinum and a hypersensitive response protein from | GAO Zhihuan XUE Yongbiao DAI Jingrui | 2001 | Chinese Science Bulletin2001，46，17： | 3 |
| 7 | Evaluation of CT findings for the differentiation of benign from malignant primary retroperitoneal tumors显示文摘 | Zhu Zheng Zhao Xinming Zhao Yanfeng Yang Lei Zhao Jing Dai Jingrui Zhou Chunwu | 2014 | Chinese Medical Journal2014，，1： | 3 |
| 8 | Angiogenesis of renal cell carcinoma: perfusion CT findings显示文摘 | Yan Chen Jin Zhang Jingrui Dai Xiaoli Feng Haizhen Lu Chunwu Zhou | 2010 | Abdominal Imaging2010，，5： | 2 |
| 9 | The pathogenic site of the C-toxin derived from Bipolaris maydis race C in maize (Zea mays)显示文摘Bipolaris maydis race C strain 523 (C523) induces severer leaf blight on cytoplasmic male sterility (CMS)-C maize than on normal (N) maize. Previously, a pathotoxin isolated from C523 (C-toxin) was shown to be responsible for the disease. To understand the basis of the differential responses between CMS-C and N maizes to this fungus, protein synthesis in vitro by mitochondria from N and CMS-C cytoplasms was monitored after their incubation in a solution containing the toxin (0.3%). Similar protein products were detected between the two alloplasmic lines, indicating that the toxin does not directly act on the mitochondrial membrane, nor inhibits the expression of mitochondrial genes. To further locate the action site of the toxin, intact leaves from both N and several subtypes of CMS-C lines were treated by 0.3% toxin. Analysis of electrolyte leakage of leaf cells showed that the leakage rates were similar to one another among the alloplasmic maize lines. In contrast, at a lower concentration of the | GAO Zhihuan XUE Yongbiao DAI Jingrui | 2000 | Chinese Science Bulletin2000，45，19： | 2 |
| 10 | Use of GFP to trace the colonization of Lactococcus lactis WH-C1 in the gastrointestinal tract of mice 显示文摘 | Wang Yanping Wang Jingrui Dai Weili | 2011 | Journal of Microbi- ological Methods2011，86，3： | 1 |
| 11 | Comparison of contrast‐enhanced sonography with MRI in the diagnosis of complex cystic renal masses显示文摘 | Yu Chen Ning Wu Tie Xue Yuzhi Hao Jingrui Dai | 2015 | J. Clin. Ultrasound2015，，4： | 1 |
| 12 | Angiogenesis of renal cell carcinoma: perfusion CT findings显示文摘 | Yan Chen Jin Zhang Jingrui Dai Xiaoli Feng Haizhen Lu Chunwu Zhou | 2010 | Abdominal Imaging2010，，5： | 1 |
| 13 | Dissection of the genetic basis of heterosis in an elite maize hybrid by QTL mapping in an immortalized F 2 population显示文摘 | Jihua Tang Jianbing Yan Xiqing Ma Wentao Teng Weiren Wu Jingrui Dai Baldev S. Dhillon Albrecht E. Melchinger Jiansheng Li | 2010 | Theoretical and Applied Genetics2010，，2： | 1 |
| 14 | Genome-wide isolation of resistance gene analogs in maize (Zea mays L.)显示文摘 | Xiao Wenkai Xu Mingliang Zhao Jiuren Wang Fengge Li Jiansheng Dai Jingrui | 2006 | Theoretical and Applied Genetics2006，，1： | 1 |
| 15 | Identification and characterization of CACTA transposable elements capturing gene fragments in maize显示文摘Transposable elements (TEs)-mediated gene sequence movement is thought to play an important role in genome expansion and origin of genes with novel functions. In this study, a gene, HGGT, involved in vitamin E synthesis was used in a case study to discover and characterize transposons carrying gene fragments in maize. A total of 69 transposons that are distributed across the 10 chromosomes and have an average length of 3689 bp were identified from the maize sequence database by using the BLAST search algorithm. Three of these carry gene fragments from the progenitor HGGT gene, while the rest (66) contain gene fragments from other cellular genes. Nine of the 69 transposons contain fragments derived from two locations in the genome. By querying the maize Expressed Sequence Tag (EST) database, we found that at least thirteen out of the 69 TEs had corresponding transcripts. More interestingly, two transposons that carry gene fragments from two different chromosomal loci could be expressed as chimeric transcripts. | LI Qing LI Lin DAI JingRui LI JianSheng YAN JianBing | 2009 | Chinese Science Bulletin2009，54，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员