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| 1 | Dual function of Arabidopsis A TAF1 in abiotic and biotic stress responses显示文摘NAC 家庭基因编码涉及多样的生物过程的植物特定的抄写因素。在这研究, Arabidopsis NAC 基因 ATAF1 被发现被干旱导致,高咸度, abscisic 酸(骆驼毛的织物) ,甲基 jasmonate,机械伤害,并且 Botrytis cinerea 感染。ATAF1 的重要正式就职在受到干旱或高咸度的骆驼毛的织物缺乏的变异的 aba2 被发现,揭示表示的骆驼毛的织物无关的机制。Arabidopsis ATAF1-overexpression 线显示了许多改变的显型,包括侏儒症和短主要的根。而且,在 vivo,实验显示 ATAF1 是真正的管理者 modulating 植物对许多不能生活的压力和 necrotrophic 病原体感染的回答。在 Arabidopsis 的 ATAF1 的 Overexpression 增加了植物敏感到骆驼毛的织物,盐,和氧化压力。特别, ATAF1 overexpression 种,然而并非异种排队显示出的显著地提高的植物忍耐到干旱。另外, ATAF1 overexpression 提高了植物危险性到 necrotrophic 病原体 B。cinerea,但是没改变 P 的无毒害或剧毒的紧张引起的疾病症状。syringae pv 西红柿 DC3000。转基因的植物 overexpressing ATAF1 对氧化应力过分敏感,建议反应的氧中介可能与响应病原体和不能生活的压力的调停 ATAF1 的发信号有关。 | Yaorong Wu Zhiyong Deng Jianbin Lai Yiyue Zhang Cuiping Yang Bojiao Yin Qingzhen Zhao Ling Zhang Yin Li Chengwei Yang Qi Xie | 2009 | Cell Research2009，19，11： | 56 |
| 2 | The role of m6A modification in the biological functions and diseases显示文摘N6-methyladenosine(m6A)is the most prevalent,abundant and conserved internal cotranscriptional modification in eukaryotic RNAs,especially within higher eukaryotic cells.m6A modification is modified by the m6A methyltransferases,or writers,such as METTL3/14/16,RBM15/15B,ZC3H3,VIRMA,CBLL1,WTAP,and KIAA1429,and,removed by the demethylases,or erasers,including FTO and ALKBH5.It is recognized by m6A-binding proteins YTHDF1/2/3,YTHDC1/2 IGF2BP1/2/3 and HNRNPA2B1,also known as'readers'.Recent studies have shown that m6A RNA modification plays essential role in both physiological and pathological conditions,especially in the initiation and progression of different types of human cancers.In this review,we discuss how m6A RNA methylation influences both the physiological and pathological progressions of hematopoietic,central nervous and reproductive systems.We will mainly focus on recent progress in identifying the biological functions and the underlying molecular mechanisms of m6A RNA methylation,its regulators and downstream target genes,during cancer progression in above systems.We propose that m6A RNA methylation process offer potential targets for cancer therapy in the future. | Xiulin Jiang Baiyang Liu Zhi Nie Lincan Duan Qiuxia Xiong Zhixian Jin Cuiping Yang Yongbin Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，3： | 16 |
| 3 | STUDY ON INJECTION AND IGNITION CONTROL OF GASOLINE ENGINE BASED ON BP NEURAL NETWORK显示文摘According to advantages of neural network and characteristics of operating procedures of engine, a new strategy is represented on the control of fuel injection and ignition timing of gasoline engine based on improved BP network algorithm. The optimum ignition advance angle and fuel injection pulse band of engine under different speed and load are tested for the samples training network, focusing on the study of the design method and procedure of BP neural network in engine injection and ignition control. The results show that artificial neural network technique can meet the requirement of engine injection and ignition control. The method is feasible for improving power performance, economy and emission performances of gasoline engine. | Zhang Cuiping Yang QingfoCollege of Mechanical Engineering,Taiyuan University of Technology,Taiyuan 030024, China | 2003 | Chinese Journal of Mechanical Engineering2003，16，4： | 13 |
| 4 | Kinetic studies of gas hydrate formation with low-dosage hydrate inhibitors显示文摘Pipeline blockage by gas hydrates is a serious problem in the petroleum industry.Low-dosage inhibitors have been developed for its cost-effective and environmentally acceptable characteristics.In a 1.072-L reactor with methane,ethane and propane gas mixture under the pressure of about 8.5 MPa at 4 °C,hydrate formation was investigated with low-dosage hydrate inhibitors PVP and GHI1,the change of the compressibility factor and gas composition in the gas phase was analyzed,the gas contents in hydrates were compared with PVP and GHI1 added,and the inhibition mechanism of GHI1 was discussed.The results show that PVP and GHI1 could effectively inhibit the growth of gas hydrates but not nucleation.Under the experimental condition with PVP added,methane and ethane occupied the small cavities of the hydrate crystal unit and the ability of ethane entering into hydrate cavities was weaker than that of methane.GHI1 could effectively inhibit molecules which could more readily form hydrates.The ether and hydroxy group of diethylene glycol monobutyl ether have the responsibility for stronger inhibition ability of GHI1 than PVP. | TANG CuiPing 1,2,DAI XingXue 1,2,DU JianWei 1,2,LI DongLiang 1,2,ZANG XiaoYa 1,2,YANG XiangYang 1 & LIANG DeQing 1 1 Guangzhou Institute of Energy Conversion,Key Laboratory of Renewable Energy and Gas Hydrate,Chinese Academy of Sciences,Guangzhou 510640,China 2 Graduate University of the Chinese Academy of Sciences,Beijing 100039,China | 2010 | Science China Chemistry2010，53，12： | 9 |
| 5 | Smoothened transduces Hedgehog signal by forming a complex with Evc/Evc2显示文摘发信号的刺猬(Hh ) 在种类从果蝇到哺乳动物在胚胎的开发和成年织物动态平衡起枢轴的作用。Hh 信号是由 Smoothened (Smo ) 的 transduced,与 G 蛋白质有关的 seven-transmembrane 蛋白质联合了受体。尽管有 Hh 在果蝇和哺乳动物由激活 Smo 的保存机制,哺乳动物的 Hh 信号怎么是从 Smo 的 transduced 到 Gli 抄写因素,糟糕被理解。这里,我们提供证据二睫的蛋白质, Evc 和 Evc2,为 Ellis 货车 Creveld 症候群负责的人的疾病基因的产品, Smo 下游地行动到 transduce Hh 信号。我们发现 Evc/Evc2 的损失不影响声音的导致刺猬的 Smo phosphorylation 和睫的本地化,但是阻碍 Hh 小径激活由 Smo 的组成地活跃的形式调停了。Evc/Evc2 为在 Sufu −/− 房间的组成的 Gli 活动是非必需的,建议支持 Gli 激活的 Sufu 在上游的那 Evc/Evc2 幕。而且,我们证明 Hh 刺激 Evc/Evc2 的绑定到取决于 Smo C 终端细胞内部的尾巴的 phosphorylation 的 Smo 并且绑定在 Kif3a −/− 被废除眼睫毛缺乏的房间。我们建议 Hh 由导致它的 phosphorylation 激活 Smo,它招募由在主要的睫反对 Sufu 激活 Gli 蛋白质的 Evc/Evc2。 | Cuiping Yang Wenlin Chen Yongbin Chen Jin Jiang | 2012 | Cell Research2012，22，11： | 8 |
| 6 | Cross-cultural adaption and psychometric properties of the Chinese version of the Diabetes Behavior Rating Scale: a pilot study显示文摘Self-care behavior plays a major role in diabetic management. However, in China, a satisfactory instrument has not yet been developed to evaluate the compliance of self-care behavior for young patients with type 1 diabetes mellitus(T1 DM). The Diabetes Behavior Rating Scale(DBRS) has a potential to be the first mature instrument. The purpose of this study is to cross-culturally adapt the DBRS, and preliminarily evaluate its psychometric properties. The instrument translation included translation, back translation and culture adaptation. Psychometric properties were assessed in a sample of 116 young patients with T1 DM adapting insulin injection therapy. The Chinese version of the DBRS was divided to four subscales. Cronbach's α for the total scale was 0.92. The mean inter-item and item-total correlations were 0.35 and 0.54 respectively. Test-retest reliability showed good temporal stability(r=0.81, P=0.001). Negative correlations were found between DBRS scores with the Diabetes Distress Scale scores(r=.0.32, P=0.003) and hemoglobin A1 c(HbA1 c) levels(r=.0.36, P=0.002). Higher DBRS scores correlated with better glycemic control. The Chinese insulin injection therapy version of the DBRS is well translated and culturally adapted. It shows good overall reliability and validity and appears to be a valuable tool for assessing the diabetic self-care behaviors for young patients with T1 DM. | Jing Zhu Jingjing Xu Yang Chen Yong Gu Li Ji Yufeng Zhou Min Zhu Hsiang-Ting Hsu Xiaoping Huang Cuiping Yuan Yun Shi Dan Yan Lili Xie Shuang Chen Tao Yang Wei He | 2018 | Science China(Life Sciences)2018，61，3： | 6 |
| 7 | Differences in brain structure in patients with distinct sites of chronic pain:A voxel-based morphometric analysis显示文摘A reduction in gray matter volume is common in patients with chronic back pain,and different types of pain are associated with gray matter abnormalities in distinct brain regions.To examine differences in brain morphology in patients with low back pain or neck and upper back pain,we investigated changes in gray matter volume in chronic back pain patients having different sites of pain using voxel-based morphometry.A reduction in cortical gray matter volume was found primarily in the left postcentral gyrus and in the left precuneus and bilateral cuneal cortex of patients with low back pain.In these patients,there was an increase in subcortical gray matter volume in the bilateral putamen and accumbens,right pallidum,right caudate nucleus,and left amygdala.In upper back pain patients,reduced cortical gray matter volume was found in the left precentral and left postcentral cortices.Our findings suggest that regional gray matter volume abnormalities in low back pain patients are more extensive than in upper back pain patients.Subcortical gray matter volume increases are found only in patients with low back pain. | Cuiping Mao Longxiao Wei Qiuli Zhang Xia Liao Xiaoli Yang Ming Zhang | 2013 | Neural Regeneration Research2013，8，32： | 6 |
| 8 | Effects of intercropping on rhizosphere soil microorganisms and root exudates of Lanzhou lily(Lilium davidii var.unicolor)显示文摘Both yield and quality of Lanzhou lily(Lilium davidii var. unicolor) are seriously affected by continuous cropping. We attempted to understand the effects of intercropping on the obstacles associated with continuous cropping of Lanzhou lily(Lilium davidii var. unicolor). The changes of rhizosphere microbial biomass and diversity in interplanting and monoculturing systems were studied by using the Illumina Hi Seq sequencing technique. The contents and composition of lily root exudates were measured by gas chromatography–mass spectrometer(GC–MS). The intercropping results of Lanzhou lily showed:(1) There was no difference in the composition of the rhizosphere soil microbes at the phylum level, but the relative abundance of the microbes decreased; and the relative abundance of harmful fungi such as Fusarium sp. increased. The relative abundance of Pleosporales sp. and other beneficial bacteria were reduced. After OTU(operational taxonomic unit)clustering, there were some beneficial bacteria, such as Chaetomium sp., in the lily rhizosphere soil in the interplanting system that had not existed in the single-cropping system. We did not find harmful bacteria that had existed in the single-cropping systm in the rhizosphere soil of interplanting system. The above results indicated that the changes of relative abundance of soil fungi and bacteria in lily rhizosphere soil was not conducive to improving the ecological structure of rhizosphere soil microbes. At the same time, the microbial composition change is very complex—beneficial and yet inadequate at the same time.(2) Root exudates provide a matrix for the growth of microorganisms. Combined with the detection of root exudates, the decrease in the composition of the root exudates of the lily was probably the reason for the decrease of the relative abundance of microbes after intercropping. At the same time, the decrease of the relative content of phenolic compounds, which inhibit the growth of microorganisms, did not increase the relative content of rhizosphere soil microorganisms. Changes in amino acids and total sugars may be responsible for the growth of Fusarium sp.. The results showed that the intercropping pattern did not noticeably alleviate the obstacle to continuous cropping of Lanzhou lily, and the change of microbial biomass and diversity was even unfavorable. However, the emergence of some beneficial bacteria, the disappearance of harmful fungi, and other changes with intercropping are in favor of alleviation of obstacles to continuous cropping of Lanzhou lily. | CuiPing Hua YaJun Wang ZhongKui Xie ZhiHong Guo YuBao Zhang Yang Qiu Le Wang | 2018 | Research in Cold and Arid Regions2018，10，2： | 5 |
| 9 | Mineral photoelectrons and their implications for the origin and early evolution of life on Earth显示文摘Energy is the key issue of all life activities.The energy source and energy yielding pathway are the key scientific issues of the origin and early evolution of life on Earth.Current researches indicate that the utilization of solar energy in large scale by life was an important breaking point of the early evolution of life on Earth and afterwards life gradually developed and flourished.However,in the widespread biochemical electron transfer of life activities,it is still not clear whether the electron source is sun or how electrons originated from sun.For billions of years,the ubiquitous semiconducting minerals in epigeosphere absorb solar energy,forming photoelectrons and photoholes.In reductive and weak acidic environment of early Earth,when photoholes were easily scavenged by reducing matters,photoelectrons were separated.Photoelectrons could effectively reduce carbon dioxide to organic matters,possibly providing organic matter foundation for the origin of life.Photoelectrons participated in photoelectron transfer chains driven by potential difference and transfer into primitive cells to maintain metabolisms.Semiconducting minerals,by absorbing ultraviolet,also protected primitive cells from being damaged by ultraviolet in the origin of life.Due to the continuous photoelectrons generation in semiconducting minerals and utilization by primitive cells,photoelectrons from semiconducting minerals’photocatalysis played multiple roles in the origin of life on early Earth,such as organic synthesis,cell protection,and energy supply.This mechanism still plays important roles in modern Earth surface systems. | LU AnHuai WANG Xin LI Yan DING HongRui WANG ChangQiu ZENG CuiPing HAO RuiXia YANG XiaoXue | 2014 | Science China Earth Sciences2014，57，5： | 5 |
| 10 | Comparative expression analysis of three genes from the Arabidopsis vacuolar Na^+/H^+ antiporter (AtNHX) family in relation to abiotic stresses显示文摘Na^+/H^+ antiporters (NHX) are ubiquitous transmembrane proteins that play a key role in salt tolerance of plants. In this study, the sequence of 3 Arabidopsis NHX gene (AtNHX2―4) were compared with other AtNHX members. Putative cis-elements analysis identified elements that have been associated with stress responses. The activities of the promoters AtNHX2―4 were studied in transgenic plants carrying corresponding promoter-β-glucuronidase (GUS) fusions. The AtNHX2 promoter-GUS analysis indicated that AtNHX2 was expressed in constitutive pattern with high GUS activity in roots and leaves. AtNHX2 promoter activity was not up-regulated by NaCl or abscisic acid (ABA), in contrast to the AtNHX1 promoter which was previously studied. The AtNHX3 and AtNHX4 promoters showed tissue-specific activities. Strong GUS activity was detected in roots and vascular bundles of the stele in plants carry-ing an AtNHX4 promoter-GUS fusion, and GUS activity increased under salt stress suggesting a func-tion related to salt tolerance. Transgenic plants carrying the AtNHX3 promoter-GUS fusion showed strong GUS activity in petals, stamens and tops of siliques, suggesting a possible role of AtNHX3 in flower and seed development. Results of histochemical analysis suggested that AtNHX2―4 are involved in divergent functions and are differentially regulated under abiotic stress. The structure of AtNHX4 was predicted to include 12 transmembrane regions and a NHX domain. Overexpression of AtNHX4 in Arabidopsis transgenic lines confers greater salt tolerance than in wild type plants. These results suggest that AtNHX4 may encode a putative vacuolar NHX that plays an important role in salt tolerance. | WANG WeiQuan LI Yin ZHANG YiYue YANG CuiPing ZHENG NuoYan XIE Qi | 2007 | Chinese Science Bulletin2007，52，13： | 5 |
| 11 | Numerical study of shoreline changes by emergency beach nourishment project at the Middle Beach of Beidaihe, China显示文摘Beach nourishment is a common coastal management strategy used to protect beach from erosion along the sandy coastlines. This method has been successfully applied to an emergency project at the West Beach of Beidaihe in the summer of 2008 and the full West Beach nourishment project in 2009-2010, which is the direct base of this study. Some basic information about the emergency engineering area at the Middle Beach is firstly described. The shoreline change of this area, including the analysis of beach width in five monitoring profiles in the bathing places of Middle Beach, is then discussed. After that a numerical model based on one-line theory is established and the numerical results agree well with the measured shorelines, which indicates that the model is appropriate and is qualified to predict the shoreline change of the Middle Beach. With the same model and parameters, long-term performance of the project is predicted, and the result shows that without follow-up nourishment and project, the bathing places can remain suitable for bathing for about 10 a. It is suggested to nourish the beach in time and carry out the beach nourishment project for the full Middle Beach in Beidaihe. | ZHANG Yu HE Lulu KUANG Cuiping PAN Yi GU Jie YANG Yanxiong ZHANG Jiabo | 2012 | Acta Oceanologica Sinica2012，31，1： | 4 |
| 12 | Effect of aging treatment on microstructure and corrosion behavior of Al-Zn-Mg aluminum alloy in aqueous solutions with different aggressive ions显示文摘The microstructure and corrosion behavior of Al-Zn-Mg alloy(namely 7×××)after natural aging treatment(NAT)and artificial aging treatment(AAT)in aqueous NaCl solutions containing different aggressive ions have been investigated in current work.Results of microstructure characterization demonstrate that the aging treatment has a great influence on the grain size and precipitates.The grain size is relatively sizeable and no evident precipitates are observed in alloy after NAT comparable with that after AAT.The electrochemical corrosion behavior of alloy was studied by polarization curve and electrochemical impedance spectroscopy(EIS).The corrosion potential(E_(corr))of the aluminum alloy is more negative in 3.5 wt.%NaCl containing 0.052 wt.%NaHSO_(3)solution than that in 3.5 wt.%NaCl solutions with or without 0.907 wt.%NaHCO_(3).Charge transfer resistance(Rct)results reveal that alloy after AAT has an enhancement of corrosion resistance compare with that after NAT.With the immersion time increasing,mostly pitting spreads over the surface of the alloy only in NaCl solution,whereas exfoliation corrosion mainly occurs in NaCl solutions containing NaHSO_(3)or NaHCO_(3). | Pan Liu Lulu Hu Qinhao Zhang Cuiping Yang Zuosi Yu Jianqing Zhang Jiming Hu Fahe Cao | 2021 | Journal of Materials Science & Technology2021，，5： | 3 |
| 13 | Biocompatible, small-sized and well-dispersed gold nanoparticles regulated by silk fibroin fiber from Bombyx mori cocoons显示文摘Biocompatible, small-sized but well-dispersed gold nanoparticles (Au NPs) remain a major challenge for their synthesis. Here a convenient solution impregnation technique is developed to prepare such Au NPs under the regulation of degummed silk fibroin fibers (SFFs) extracted from Bombyx mori cocoons. SFFs play multiple roles in the formation of Au NPs such as reactive substrate to capture AuCl4^- ions by the chelation of -C = 0, reducing agent for Au(0) by the reduction of -OH , and modifiers to render biocompatible Au NPs by some functional groups and biomolecules. The as-prepared Au NPs with a size of 7-10 nm are embedded in the solid SFF substrate, and can disperse well in the liquid system by the disintegration of SFFs into silk fibroin (SF) in a certain CaCl2 solution. The biocompatible Au NPs exhibit uniform small size and distribute stably in both solid and solution states, which have distinctive properties and functional advantages, and bring great convenience to their storage and transportation. | Chengzhi YANG Shikun CHEN Huilan SU Haoyue ZHANG Jianfei TANG Cuiping GUO Fang SONG Wang ZHANG Jiajun GU Qinglei LIU | 2019 | Frontiers of Materials Science2019，13，2： | 2 |
| 14 | Role ofβ(FeA1)nanoparticles in abnormal grain growth in the annealing of cast Cu-Al-Mn-Fe shape memory alloys显示文摘In this study,the low-cost production of Cu-AI-Mn-Fe shape memory alloy single crystals exceeding 46 mm by abnormal grain growth was realized only through annealing their cast alloys.The results show that the mis-orientation formed during annealing may be responsible for such abnormal grain growth process.It was con-firmed that this misorientation resulted from the dissolution of bec B(FeAI)nanoparticles during heat treatment at a sufficiently temperature of approximately 1173 K.The rate of migration of the abnormal grain boundary was experimentally measured to be approximately9.3 x 10^-m s^-1within 2 min of the commencement of abnormal grain growth.Additionally,the range of composition of the alloys that can lead to abnormal grain growth was determined.When the Cu-13.0AI-6.5Mn-3.2Fe single crystal close to the[100]direction was deformed to a pre-strain of 12%,full shape recovery happened without any residual strain.At that time,the superelastic strain was approximately 9%.Such a superelastic characteristic remained nearly constant over 50 cycles,showing excellent fatigue resistance.The superelastic properties of the present Cu-13.0AI-6.5Mn-3.2Fe single crystal are compared to those of commercial Ni-Ti-based shape memory alloys.Therefore,it can be considered as a new kind of superelastic material having practical applications.The obtained results should be of great significance in the development of Cu based shape memory alloys.Furthermore,it is expected that a similar microstructure can be designed for the production of more metallic single crystals. | Shuiyuan Yang Xinyu Qing Jixun Zhang Lipeng Guo Shen Hong Mingpei Li Jinbin Zhang Cuiping Wang Xingjun Liu | 2020 | Progress in Natural Science:Materials International2020，30，4： | 2 |
| 15 | Phase Boundary Mapping in ZrNiSn Half-Heusler for Enhanced Thermoelectric Performance显示文摘The solubility range of interstitial Ni in the ZrNi1+xSn half-Heusler phase is a controversial issue,but it has an impact on the thermoelectric properties.In this study,two isothermal section phase diagrams of the Zr-Ni-Sn ternary system at 973K and 1173 K were experimentally constructed based on the binary phase diagrams of Zr-Ni,Zr-Sn,and Ni-Sn.The thermodynamic equilibrium phases were obtained after a long time of heating treatment on the raw alloys prepared by levitation melting.Solubilities of x<0:07 at 973 K and x<0:13 at 1173 K were clearly indicated.An intermediate-Heusler phase with a partly filled Ni void was observed,which is believed to be beneficial to the lowered lattice thermal conductivity.The highest ZT value~0:71 at 973 K was obtained for ZrNi_(1.11)Sn_(1.04).The phase boundary mapping provides an important instruction for the further optimization of ZrNiSn-based materials and other systems. | Xiaofang Li Pengbo Yang Yumei Wang Zongwei Zhang Dandan Qin Wenhua Xue Chen Chen Yifang Huang Xiaodong Xie Xinyu Wang Mujin Yang Cuiping Wang Feng Cao Jiehe Sui Xingjun Liu Qian Zhang | 2020 | Research2020，，1： | 2 |
| 16 | Wendan decoction improves learning and memory deficits in a rat model of schizophrenia显示文摘An experimental model of schizophrenia was established using dizocilpine (MK-801). Rats were intragastrically administered with Wendan decoction or clozapine for 21 days prior to establishing the model. The results revealed that the latency of schizophrenia model rats to escape from the hidden platform in the Morris water maze was significantly shortened after administration of Wendan decoction or clozapine. In addition, the treated rats crossed the platform significantly more times than the untreated model rats. Moreover, the rate of successful long-term potentiation induction in the Wendan decoction group and clozapine group were also obviously increased compared with the model group, and the population spike peak latency was significantly shortened. These experimental findings suggest that Wendan decoction can improve the learning and memory ability of schizophrenic rats to the same extent as clozapine treatment. | Cuiping Yang Changchun Cai Xiaojin Yang Yanping Yang Zhigang Zhou Jianhua Liu Heping Ye HongjiaoWan | 2012 | Neural Regeneration Research2012，7，15： | 2 |
| 17 | Metal deformation in skew rolling step-shaft显示文摘The process of rolling step-shaft was simulated with the help of the FEM (Finite-Element Model) software-DEFORMTM 3D. The characteristics of metal flow in different periods were analyzed, and the curve of variety in non-round area at different crosssections of the rolled piece, the curve of variety in bulge volume and the curve of variety in non-column volume were obtained. On this base the method of tool design is worked out and validated by experiment. | Yongqiang Kang, Jinping Liu, Cuiping Yang, and Zhenghuan HuMechanical Engineering School, University of Science and Technology Beijing, Beijing 100083, China | 2003 | Journal of University of Science and Technology Beijing2003，10，5： | 2 |
| 18 | MiR146a-loaded engineered exosomes released from silk fibroin patch promote diabetic wound healing by targeting IRAK1显示文摘Unhealable diabetic wounds need to be addressed with the help of newer,more efficacious strategies.Exosomes combined with biomaterials for sustained delivery of therapeutic agents are expected to bring new hope for chronic wound treatment.Here,the engineered exosomes modified for efficiently loading miR146a and attaching to silk fibroin patch(SFP)were demonstrated to promote diabetic wound healing.Silk fibroin binding peptide(SFBP)was screened through phage display,and SFBP-Gluc-MS2(SGM)and pac-miR146a-pac fusion protein were constructed.The designed exosomes(SGM-Exos,miR146a-Exos,and SGM-miR146a-Exos)were isolated from the engineered placental mesenchymal stem cells(PMSCs)transduced with SGM or/and pac-miR146a-pac protein.Gluc signals indicated SGM-Exo@SFP markedly increased the binding rate and the stability of SGM-Exo.Moreover,the loading efficiency of miR146a in SGM-miR146a-Exos was ten-fold higher than that in miR146a-Exos.Superior to untreated,SGM-miR146a-Exo-only treated,and SFP-only treated groups,SGM-miR146a-Exo@SFP drived wound healing associated with less inflammation,collagen deposition,and neovascularization.The transcriptomics analysis suggested anti-inflammatory and regenerative effects with SGM-miR146a-Exo@SFP treatment.Here,we show efficient exosome@biomaterial-based miRNA delivery systems for regenerative medicine and tissue engineering. | Qiankun Li Wenzhi Hu Qilin Huang Jie Yang Bingmin Li Kui Ma Qian Wei Yaxi Wang Jianlong Su Mengli Sun Shengnan Cui Rungong Yang Haihong Li Xiaobing Fu Cuiping Zhang | 2023 | Signal Transduction and Targeted Therapy2023，8，3： | 2 |
| 19 | Inhibition of Breast Cancer Resistance Protein(BCRP) by Ko143 Can Affect Pharmacokinetics of Enrofloxacin in Exopalaemon carinicauda显示文摘Adenosine triphosphate-binding cassette transporter breast cancer resistance protein(BCRP) exists highly in the apical membranes of epithelia, and is involved in drug availability. Ko143 is a typical inhibitor of BCRP in rodents. The synthetic antibacterial agent enrofloxacin(ENRO) is a fluoroquinolone employed as veterinary and aquatic medicine, and also a substrate for BCRP. BCRP gene highly expressed in the hepatopancreas and intestine of Exopalaemon carinicauda as was determined with real-time quantitative reverse transcription-polymerase chain reaction(RT-q PCR) method. The effects of Ko143 on the abundance of BCRP m RNA and ENRO pharmacokinetics in E. carinicauda were studied. The m RNA abundance of BCRP decreased significantly in hepatopancreas and intestine(P < 0.05) after Ko143 treatment. Co-administration of Ko143 significantly changed the pharmacokinetics of orally administered enrofloxacin, which was supported by higher distribution half-life(t_(1/2α)), elimination half-life(t_(1/2β)), area under the curve up to the last measurable concentration(AUC_(0-t)), peak concentration(C_(max)) and lower clearance(CL/F). These findings revealed that Ko143 downregulates BCRP expression in hepatopancreas and intestine, thus affects the pharmacokinetics of orally administered enrofloxacin in E. carinicauda. The drug-drug interaction can be caused by the change in BCRP activity if ENRO is used in combination with other drugs in shrimp. | ZHAI Qianqian XU Yang LI Cuiping FENG Yanyan CUI Yanting MA Li LI Jian | 2020 | Journal of Ocean University of China2020，19，5： | 2 |
| 20 | mTOR regulate EMT through RhoA and Rac1 pathway in prostate cancer显示文摘 | XianGuo Chen HaiYan Cheng TengFei Pan Yi Liu Yang Su CuiPing Ren DaKe Huang XiaoJun Zha ChaoZhao Liang | 2015 | Mol. Carcinog2015，，10： | 1 |
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