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| 1 | Effects of intercropping on rhizosphere soil microorganisms and root exudates of Lanzhou lily(Lilium davidii var.unicolor)显示文摘Both yield and quality of Lanzhou lily(Lilium davidii var. unicolor) are seriously affected by continuous cropping. We attempted to understand the effects of intercropping on the obstacles associated with continuous cropping of Lanzhou lily(Lilium davidii var. unicolor). The changes of rhizosphere microbial biomass and diversity in interplanting and monoculturing systems were studied by using the Illumina Hi Seq sequencing technique. The contents and composition of lily root exudates were measured by gas chromatography–mass spectrometer(GC–MS). The intercropping results of Lanzhou lily showed:(1) There was no difference in the composition of the rhizosphere soil microbes at the phylum level, but the relative abundance of the microbes decreased; and the relative abundance of harmful fungi such as Fusarium sp. increased. The relative abundance of Pleosporales sp. and other beneficial bacteria were reduced. After OTU(operational taxonomic unit)clustering, there were some beneficial bacteria, such as Chaetomium sp., in the lily rhizosphere soil in the interplanting system that had not existed in the single-cropping system. We did not find harmful bacteria that had existed in the single-cropping systm in the rhizosphere soil of interplanting system. The above results indicated that the changes of relative abundance of soil fungi and bacteria in lily rhizosphere soil was not conducive to improving the ecological structure of rhizosphere soil microbes. At the same time, the microbial composition change is very complex—beneficial and yet inadequate at the same time.(2) Root exudates provide a matrix for the growth of microorganisms. Combined with the detection of root exudates, the decrease in the composition of the root exudates of the lily was probably the reason for the decrease of the relative abundance of microbes after intercropping. At the same time, the decrease of the relative content of phenolic compounds, which inhibit the growth of microorganisms, did not increase the relative content of rhizosphere soil microorganisms. Changes in amino acids and total sugars may be responsible for the growth of Fusarium sp.. The results showed that the intercropping pattern did not noticeably alleviate the obstacle to continuous cropping of Lanzhou lily, and the change of microbial biomass and diversity was even unfavorable. However, the emergence of some beneficial bacteria, the disappearance of harmful fungi, and other changes with intercropping are in favor of alleviation of obstacles to continuous cropping of Lanzhou lily. | CuiPing Hua YaJun Wang ZhongKui Xie ZhiHong Guo YuBao Zhang Yang Qiu Le Wang | 2018 | Research in Cold and Arid Regions2018，10，2： | 5 |
| 2 | Protective Effect of Holothurian Intestine Against Indomethacin Induced Gastric Mucosal Damage in Rats显示文摘Our study aimed to investigate the protective effects of Holothurian intestines(HI) on NSAIDs-induced gastric mucosal damage and the possible mechanism. At first, 60 male Wistar rats were induced of gastric lesions with indomethacin(IDM, 30 mg kg^(-1)). The rats were pretreated for 15 consecutive days with saline, sucralfate, or HI(0.4g kg^(-1) d-1, 0.8 g kg^(-1) d^(-1) and 1.6 g kg^(-1) d^(-1)) prior to IDM treatment, followed by evaluations of macroscopic damage and microscopic features; and investigation of the levels of inflammatory cytokines, oxidative stress parameters, gastric mucosal prostaglandin E2(PGE2) and total hexosamine in tissues. The expression of COX-1 and COX-2 m RNA in the gastric tissue were determined by quantitative polymerase chain reaction(q PCR). Pathological gastric ulcer indexes, levels of pro-inflammatory cytokines(IL-1β, IL-17, TNF-α) and lipid peroxidation were significantly decreased in HI-treated groups, whereas the levels of protective factors(TGF-β, GSH, SOD activity and PGE2) were significantly elevated especially in the group with HI 1.61 g kg^(-1) d^(-1)(P < 0.05). Furthermore, the expression of COX-2 mRNA decreased significantly in HI groups(P < 0.05). The study investigates that holothurian intestines may act as a kind of marine medicine which have protective effect on IDM-induced gastric ulcer, which could be a dietary preventive agent for the prevention of gastric damage. | LI Xiaoyu QIAO Xuejing ZHANG Cuiping GAO Hua NIU Qinghui WU Tong ZHANG Qi TIAN Zibin | 2017 | Journal of Ocean University of China2017，16，3： | 4 |
| 3 | The Global Landscape of SARS-CoV-2 Genomes, Variants, and Haplotypes in 2019nCoVR显示文摘On January 22,2020,China National Center for Bioinformation(CNCB)released the 2019 Novel Coronavirus Resource(2019nCoVR),an open-access information resource for the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).2019nCoVR features a comprehensive integration of sequence and clinical information for all publicly available SARS-CoV-2 isolates,which are manually curated with value-added annotations and quality evaluated by an automated in-house pipeline.Of particular note,2019nCoVR offers systematic analyses to generate a dynamic landscape of SARS-CoV-2 genomic variations at a global scale.It provides all identified variants and their detailed statistics for each virus isolate,and congregates the quality score,functional annotation,and population frequency for each variant.Spatiotemporal change for each variant can be visualized and historical viral haplotype network maps for the course of the outbreak are also generated based on all complete and high-quality genomes available.Moreover,2019nCoVR provides a full collection of SARS-CoV-2 relevant literature on the coronavirus disease 2019(COVID-19),including published papers from PubMed as well as preprints from services such as bioRxiv and medRxiv through Europe PMC.Furthermore,by linking with relevant databases in CNCB,2019nCoVR offers data submission services for raw sequence reads and assembled genomes,and data sharing with NCBI.Collectively,SARS-CoV-2 is updated daily to collect the latest information on genome sequences,variants,haplotypes,and literature for a timely reflection,making 2019nCoVR a valuable resource for the global research community.2019nCoVR is accessible at http://gffzzdbc7b6aaae734bddsx9pw9cc6nvux6uxk.ffgz.tsg.suse.edu.cn/ncov/. | Shuhui Song Lina Ma Dong Zou Dongmei Tian Cuiping Li Junwei Zhu Meili Chen Anke Wang Yingke Ma Mengwei Li Xufei Teng Ying Cui Guangya Duan Mochen Zhang Tong Jin Chengmin Shi Zhenglin Du Yadong Zhang Chuandong Liu Rujiao Li Jingyao Zeng Lili Hao Shuai Jiang Hua Chen Dali Han Jingfa Xiao Zhang Zhang Wenming Zhao Yongbiao Xue Yiming Bao | 2020 | Genomics, Proteomics & Bioinformatics2020，18，6： | 4 |
| 4 | Uneven aftershock distribution of Wenchuan Ms8.0 earthquake and possible mechanism显示文摘The aftershock activity of Wenchuan Ms8.0 earthquake showed different spatial and temporal distributions along two different segments of the Longmenshan fault.This difference was likely the result of segmentation of the earthquake rupture process,which in turn may be the result of the fault's segmentation in its long-term geotectonic condition. | Li Zhixiong Chen Zhangli Zhao Cuiping Shao Zhigang Hua Wei Wang Qincai He Ping | 2011 | Geodesy and Geodynamics2011，2，2： | 1 |
| 5 | Measurement of Earthquake Size显示文摘The paper begins with a brief review of the research history of earthquake size measurement. On this basis,the author pointed out the following points: 1 In recent decades,ML,mb( mB),MSmagnitude scales are widely used as measures of earthquake size. However,these magnitude scales have a deficiency of 'overgeneralization ' and'magnitude saturation'. Moreover,since they do not fully take into account the regional difference of seismic attenuation, especially the difference of site effects on the amplification of ground motion,these magnitude scales are but inaccurate measures of earthquake size. 2 Seismic moment M0 not only has clear physical meaning,but also overcomes the deficiencies existing in ML,mb( mB) and MSmagnitude scales,so it is the most suitable physical quantity for measuring earthquake size scientifically. In order to continue to use the term 'magnitude',Kanamori defined the moment magnitude scaleΔσ. Although its prerequisite assumptions remain to be studied,it is still a reasonable scale used as a measure of the relative size of an earthquake. 3 For measuring the earthquake size more scientifically,we must make full use of a large amount of waveform data from modern regional digital seismograph networks,strengthen the research on seismic wave attenuation characteristics,site effect,calculation of source parameters and the related scaling relations. In improving the measurement methods for ML,mb( mB) and MSmagnitude,we should focus on the improvement of MWscale and carry forward the work as gradually taking MWmagnitude scale as the uniform physical quantity to measure the relative size of earthquakes,so as to lay a more solid foundation for research in earthquake science and earthquake prediction. | Chen Zhangli Chen Hanlin Zhao Cuiping Wang Qincai Hua Wei Zhou Lianqing | 2014 | Earthquake Research in China2014，28，3： | 0 |
| 6 | Genomic Epidemiology of SARS-CoV-2 in Pakistan显示文摘COVID-19 has swept globally and Pakistan is no exception.To investigate the initial introductions and transmissions of the SARS-CoV-2 in Pakistan,we performed the largest genomic epidemiology study of COVID-19 in Pakistan and generated 150 complete SARS-CoV-2 genome sequences from samples collected from March 16 to June 1,2020.We identified a total of 347 mutated positions,31 of which were over-represented in Pakistan.Meanwhile,we found over 1000 intra-host single-nucleotide variants(iSNVs).Several of them occurred concurrently,indicating possible interactions among them or coevolution.Some of the high-frequency iSNVs in Pakistan were not observed in the global population,suggesting strong purifying selections.The genomic epidemiology revealed five distinctive spreading clusters.The largest cluster consisted of 74 viruses which were derived from different geographic locations of Pakistan and formed a deep hierarchical structure,indicating an extensive and persistent nation-wide transmission of the virus that was probably attributed to a signature mutation(G8371T in ORF1ab)of this cluster.Furthermore,28 putative international introductions were identified,several of which are consistent with the epidemiological investigations.In all,this study has inferred the possible pathways of introductions and transmissions of SARS-CoV-2 in Pakistan,which could aid ongoing and future viral surveillance and COVID-19 control. | Shuhui Song Cuiping Li Lu Kang Dongmei Tian Nazish Badar Wentai Ma Shilei Zhao Xuan Jiang Chun Wang Yongqiao Sun Wenjie Li Meng Lei Shuangli Li Qiuhui Qi Aamer Ikram Muhammad Salman Massab Umair Huma Shireen Fatima Batool Bing Zhang Hua Chen Yun-Gui Yang Amir Ali Abbasi Mingkun Li Yongbiao Xue Yiming Bao | 2021 | Genomics, Proteomics & Bioinformatics2021，19，5： | 0 |
| 7 | Long-term outcomes and prognosis factors of vagus nerve stimulation in patients with refractory epilepsy显示文摘Background:Vagus nerve stimulation(VNS)is an effective treatment for patients with refractory epilepsy,yet with varied predictive factors and heterogeneous long-term outcomes.Adjustment of VNS parameters is critical for obtaining favorable efficacy.In this study,we aimed to investigate the long-term outcomes and the possible predictive factors of VNS in patients with refractory epilepsy.Methods:Eighty-six patients(59 males and 27 females)who underwent VNS implantation for treatment of refractory epilepsy between May 2016 and May 2017 at five Epilepsy Centers were enrolled.The clinical data,including sex,age at epilepsy onset,VNS implantation,epilepsy duration,seizure type,MRI findings,history of neurosurgical operations,and responder rate(responders were those with≥50%seizure reduction),were analyzed.Results:Four-year follow-up data were available for 76 patients(53 males and 23 females).The mean current intensity at the last follow-up was 1.8±0.3 mA(range:0.75–2.5 mA).The mean seizure reduction was 36.2%at 6 months,38.5%at 1 year,69.4%at 3 years,and 56.7%at 4 years.A favorable outcome of≥50%reduction in seizure frequency occurred in 40.0%of the patients at 6 months,55.9%at 1 year with 4 patients being seizure-free,63.2%at 3 years with 5 patients being seizure-free,and 68.4%at 4 years with 5 patients being seizure-free.Earlier onset age(P<0.001)and shorter duration(P=0.042)were associated with favorable prognosis.Compared with generalized tonicclonic seizures,tonic seizures had a favorable outcome(P=0.026).Twenty-three patients underwent neurosurgical operations before VNS implantation,and the responder rate was 60.9%at the last follow-up.Conclusions:VNS is an adjunctive and effective treatment for patients with refractory epilepsy who are not good candidates for surgical resection or have failed to respond to surgical treatment.The stimulation efficacy increases over time after implantation,and earlier exposure to VNS improves the prognosis. | Cuiping Xu Hua Lin Jiwen Xu Xiaohua Zhang Guiliang Hao Qiang Qiang Liu Chengyun Ding Shan Wang Quanjun Zhao Xiaojun Bai Kui Chen Duanyu Ni Yongjie Li Tao Yu Yuping Wang | 2022 | Acta Epileptologica2022，5，3： | 0 |
| 8 | Decomposition effects of Lanzhou lily(Lilium davidii var.unicolor)flowers on soil physical and chemical properties and microbial community diversity显示文摘Timely removal of the flower is a key agricultural measure to ensure the concentrated supply of nutrients for the growth of underground bulbs and to increase the yield of lilies. Removing flowers and returning them to the field is one of the tradi‐tional ways of treatment, and field litter is formed at this time. Previous study showed that the decomposition of litter changes the soil properties. In order to study the effects of lily litter decomposition on soil physical and chemical proper‐ties and microbial structure, three treatments were set up in reference to the Decomposition Bag Method: control (CK), Lanzhou lily flower treatment (LZF), and Zhongbai No.1 flower treatment (ZBF). The effects of lily decomposition on soil physical and chemical properties and microbial community composition were studied in order to provide a scientific basis and theoretical guidance for the planting process of Lanzhou lily. The results show that the decomposition of lily flowers significantly increased the contents of soil organic matter, soil total nitrogen, soil total phosphorus and soil avail‐able potassium, and decreased soil pH. RDA shows that soil available nutrients and pH were the driving factors for the change of the soil microbial community. A short-term change of soil microenvironment caused by the decomposed lily flower is beneficial to growing the Lanzhou lily. However, under the correlation analysis of environmental factors, the long-term effects of returning the Lanzhou lily flower to the field, such as the trend of soil acidification, need to be further studied. | Jie Li YaJun Wang Yang Qiu ZhongKui Xie YuBao Zhang CuiPing Hua | 2022 | Research in Cold and Arid Regions2022，14，3： | 0 |
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