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| 1 | Contrasting elevational diversity patterns for soil bacteria between two ecosystems divided by the treeline显示文摘Above- and below-ground organisms are closely linked, but how elevational distribution pattern of soil microbes shifting across the treeline still remains unknown. Sampling of 140 plots with transect, we herein investigated soil bacterial distribution pattern from a temperate forest up to a subalpine meadow along an elevational gradient using Illumina sequencing. Our results revealed distinct elevational patterns of bacterial diversity above and below the treeline in responding to changes in soil conditions:a hollow elevational pattern in the forest(correlated with soil temperature, pH, and C:N ratio) and a significantly decreasing pattern in the meadow(correlated with soil pH, and available phosphorus). The bacterial community structure was also distinct between the forest and meadow, relating to soil pH in the forest and soil temperature in the meadow. Soil bacteria did not follow the distribution pattern of herb diversity, but bacterial community structure could be predicted by herb community composition. These results suggest that plant communities have an important influence on soil characteristics, and thus change the elevational distribution of soil bacteria. Our findings are useful for future assessments of climate change impacts on microbial community. | Guixiang Li Guorui Xu Congcong Shen Yong Tang Yuxin Zhang Keming Ma | 2016 | Science China(Life Sciences)2016，59，11： | 9 |
| 2 | Genetic bases of source-,sink-,and yield-related traits revealed by genome-wide association study in Xian rice显示文摘The source-sink relationship determines the ultimate grain yield.We investigated the genetic basis of the relationship between source and sink and yield potential in rice.In two environments,we identified quantitative trait loci(QTL)associated with sink capacity(total spikelet number per panicle and thousand-grain weight),source leaf(flag leaf length,flag leaf width and flag leaf area),source-sink relationship(total spikelet number to flag leaf area ratio)and yield-related traits(filled grain number per panicle,panicle number per plant,grain yield per plant,biomass per plant,and harvest index)by genome-wide association analysis using 272 Xian(indica)accessions.The panel showed substantial variation for all traits in the two environments and revealed complex phenotypic correlations.A total of 70 QTL influencing the 11 traits were identified using 469,377 high-quality SNP markers.Five QTL were detected consistently in four chromosomal regions in both environments.Five QTL clusters simultaneously affected source,sink,source–sink relationship,and grain yield traits,probably explaining the genetic basis of significant correlations of grain yield with source and sink traits.We selected 24 candidate genes in the four consistent QTL regions by identifying linkage disequilibrium(LD)blocks associated with significant SNPs and performing haplotype analysis.The genes included one cloned gene(NOG1)and three newly identified QTL(qHI6,qTGW7,and qFLA8).These results provide a theoretical basis for high-yield rice breeding by increasing and balancing source–sink relationships using marker-assisted selection. | Yun Wang Yunlong Pang Kai Chen Laiyuan Zhai Congcong Shen Shu Wang Jianlong Xu | 2020 | The Crop Journal2020，8，1： | 8 |
| 3 | Cloning of Ln Gene Through Combined Approach of Map-based Cloning and Association Study in Soybean显示文摘Increasing yield is one of the most important goals in crop breeding.Soybean(Glycine max L.Merr.),one of the most economically important leguminous seed crops,provides the majority of plant proteins,and more than a quarter of the world's food and animal feed(Graham and Vance,2003). The yield of soybean is finally determined by the number of seeds per unit area,which affected by many characters,such as height,branching number,photosynthesis,seed size,seed number.The number of seeds per pod is taken for one of the critical components that related to yield(You et al.,1995),and it has long been considered in soybean production(Takahashi, | Chao Fang Weiyu Li Guiquan Li Zheng Wang Zhengkui Zhou Yanming Ma Yanting Shen Congcong Li Yunshuai Wu Baoge Zhu Weicai Yang Zhixi Tian | 2013 | Journal of Genetics and Genomics2013，40，2： | 7 |
| 4 | Genetic dissection of rice appearance quality and cooked rice elongation by genome-wide association study显示文摘Appearance and cooked rice elongation are key quality traits of rice. Although some QTL for these traits have been identified, understanding of the genetic relationship between them remains limited. In the present study, large phenotypic variation was observed in 760 accessions from the 3 K Rice Genomes Project for both appearance quality and cooked rice elongation. Most component traits of appearance quality and cooked rice elongation showed significant pairwise correlations, but a low correlation was found between appearance quality and cooked rice elongation. A genome-wide association study identified 74 QTL distributed on all 12 chromosomes for grain length, grain width, length to width ratio, degree of endosperm with chalkiness, rice elongation difference, and elongation index. Thirteen regions containing QTL stably expressed in multiple environments and/or exerting pleiotropic effects on multiple traits were detected. By gene-based association analysis and haplotype analysis, 46 candidate genes, including five cloned genes, and 49 favorable alleles were identified for these 13 QTL. The effect of the candidate gene Wx on rice elongation difference was validated by a transgenic strategy. These results shed light on the genetic bases of appearance quality and cooked rice elongation and provide gene resources for improving rice quality by molecular breeding. | Xianjin Qiu Jing Yang Fan Zhang Yanan Niu Xiuqing Zhao Congcong Shen Kai Chen Sheng Teng Jianlong Xu | 2021 | The Crop Journal2021，9，6： | 5 |
| 5 | Association of assisted reproductive technology, germline de novo mutations and congenital heart defects in a prospective birth cohort study显示文摘Emerging evidence suggests that children conceived through assisted reproductive technology(ART)have a higher risk of congenital heart defects(CHDs)even when there is no family history.De novo mutation(DNM)is a well-known cause of sporadic congenital diseases;however,whether ART procedures increase the number of germline DNM(gDNM)has not yet been well studied.Here,we performed whole-genome sequencing of 1137 individuals from 160 families conceived through ART and 205 families conceived spontaneously.Children conceived via ART carried 4.59 more gDNMs than children conceived spontaneously,including 332 paternal and 1.26 maternal DNMs,after correcting for parental age at conception,cigarette smoking,alcohol drinking,and exercise behaviors.Paternal DNMs in offspring conceived via ART are characterized by C>T substitutions at CpG sites,which potentially affect protein-coding genes and are significantly associated with the increased risk of CHD.In addition,the accumulation of non-coding functional mutations was independently associated with CHD and 87.9% of the mutations were originated from the father.Among ART offspring,infertility of the father was associated with elevated paternal DNMs;usage of both recombinant and urinary follicle-stimulating hormone and high-dosage human chorionic gonadotropin trigger was associated with an increase of maternal DNMs.In sum,the increased gDNMs in offspring conceived by ART were primarily originated from fathers,indicating that ART itself may not be a major reason for the accumulation of gDNMs.Our findings emphasize the importance of evaluating the germline status of the fathers in families with the use of ART. | Cheng Wang Hong Lv Xiufeng Ling Hong Li Feiyang Diao Juncheng Dai Jiangbo Du Ting Chen Qi Xi Yang Zhao Kun Zhou Bo Xu Xiumei Han Xiaoyu Liu Meijuan Peng Congcong Chen Shiyao Tao Lei Huang Cong Liu Mingyang Wen Yangqian Jiang Tao Jiang Chuncheng Lu Wei Wu Di Wu Minjian Chen Yuan Lin Xuejiang Guo Ran Huo Jiayin Liu Hongxia Ma Guangfu Jin Yankai Xia Jiahao Sha Hongbing Shen Zhibin Hu | 2021 | Cell Research2021，31，8： | 2 |
| 6 | Assessment of dairy cow feed intake based on BP neural network with polynomial decay learning rate显示文摘To overcome the shortcomings of traditional dairy cow feed intake assessment model andBP neural network, this paper proposes a method of optimizing BP neural network usingpolynomial decay learning rate, taking the cow’s body weight, lying duration, lying times,walking steps, foraging duration and concentrate-roughage ratio as input variables andtaking the actual feed intake is the output variable to establish a dairy cow feed intakeassessment model, and the model is trained and verified by experimental data collectedon site. For the sake of comparative study, feed intake is simultaneously assessed by SVRmodel, KNN logistic regression model, traditional BP neural network model, and multilayerBP neural network model. The results show that the established BP model using the polynomial decay learning rate has the highest assessment accuracy, the MSPE, RMSE, MAE,MAPE and R2 are 0.043 kg2/d and 0.208 kg/d, 0.173 kg/d, 1.37% and 0.94 respectively. Compared with SVR model and KNN mode, the RMSE value reduced by 43.9% and 26.5%, it isalso found that the model designed in this paper has many advantages in comparison withthe BP model and multilayer BP model in terms of precision and generalization. Therefore,this method is ready to be applied for accurately evaluating the dairy cow feed intake, andit can provide theoretical guidance and technical support for the precise-feeding and canalso be of high significance in the improvement of dairy precise-breeding. | Weizheng Shen Gen Li Xiaoli Wei Qiang Fu Yonggen Zhang Tengyu Qu Congcong Chen Runtao Wang | 2022 | Information Processing in Agriculture2022，9，2： | 2 |
| 7 | In-situ hydrophobic environment triggering reactive fluorescence probe to real-time monitor mitochondrial DNA damage显示文摘Mitochondrial DNA has a special structure that is prone to damage resulting in many serious diseases,such as genetic diseases and cancers.Therefore,the rapid and specific monitoring of mitochondrial DNA damage is urgently needed for biological recognition.Herein,we constructed an in situ hydrophobic environment-triggering reactive fluorescence probe named MBI-CN.The fluorophore was 2-styrene-1H-benzo[d]imidazole,and malononitrile was introduced as a core into a molecule to initiate the hydrolysis reaction in the specific environment containing damaged mitochondrial DNA.In this design,MBI-CN conjugates to mitochondrial DNA without causing additional damages.Thus,MBI-CN can be hydrolyzed to generate MBI-CHO in an in situ hydrophobic environment with mitochondrial DNA damage.Meanwhile,MBI-CHO immediately emitted a significative fluorescence signal changes at 437 and 553 nm within 25 s for the damaged mitochondria DNA.Give that the specific and rapid response of MBI-CN does not cause additional damages to mitochondrial DNA,it is a potentially effective detection tool for the real-time monitoring of mitochondrial DNA damage during cell apoptosis and initial assessment of cell apoptosis. | Beidou Feng Huiyu Niu Hongchen Zhai Congcong Shen Hua Zhang | 2022 | Frontiers of Chemical Science and Engineering2022，16，1： | 2 |
| 8 | Effect of Build Direction on Fatigue Performance of L-PBF 316L Stainless Steel显示文摘The microstructure and fatigue and tensile properties of 316 L stainless steel fabricated via laser powder bed fusion(L-PBF)were investigated.Two 316 L stainless steel specimens with different loading directions which are either perpendicular to or parallel to building direction were prepared by L-PBF process.The results of X-ray diffraction tomography showed that there was no significant difference in morphology and size/distribution of the defects in the HB and VB samples.Since long axis of columnar grains is generally parallel to the build direction,the fatigue crack encounters more grain boundaries in VB samples under cyclic loading,which led to enhanced fatigue resistance of VB samples compared with HB sample.In contrast to HB sample,the VB sample has a higher fatigue strength due to a higher resistance to localized plastic deformation under cyclic loading.The differences in fatigue properties of L-PBF 316 L SS with different build directions were predominantly controlled by solidification microstructures. | Chenfan Yu Yuan Zhong Peng Zhang Zhenjun Zhang Congcong Zhao Zhefeng Zhang Zhijian Shen Wei Liu | 2020 | Acta Metallurgica Sinica(English Letters)2020，33，4： | 1 |
| 9 | Genetic mechanism of heterosis for rice milling and appearance quality in an elite rice hybrid显示文摘Development of hybrid rice with high yield and grain quality is a goal of rice breeding.To investigate the genetic mechanism of heterosis for rice milling and appearance quality in indica/xian rice,QTL mapping was conducted using 1061 recombinant inbred lines(RILs)derived from a cross of the xian rice cultivars Quan 9311B(Q9311B)and Wu-shan-si-miao(WSSM),and a backcross F_(1)(BC_(1)F_(1)) population developed by crossing the RILs with Quan 9311A(Q9311A),combined with phenotyping in two environments.The F_(1) hybrid(Q9311A×WSSM)showed various degrees of heterosis for milling and appearance quality.A total of 142 main-effect QTL(M-QTL)and 407 pairs of epistatic QTL(E-QTL)were identified for five milling and appearance quality traits and grain yield per plant(GYP)in the RIL,BC_(1)F_(1) and mid-parental heterosis(H_(MP)) populations.Differential detection of QTL in three populations revealed that most additive loci detected in the RILs did not show heterotic effects,but some of them did contribute to BC_(1)F_(1) trait performance.Unlike heterosis of GYP,single-locus overdominance and epistasis were the main contributors to heterosis for milling and appearance quality.Epistasis contributed more to the heterosis for milling quality than to that for appearance quality.Three(four)QTL regions harboring opposite(consistent)directions of favorable allele effects for GYP and grain quality were identified,indicating the presence of partial genetic overlaps between GYP and grain quality.Three strategies are proposed to develop hybrid rice with high yield and good grain quality:1)pyramiding favorable alleles with consistent directions of gene effects for GYP and grain quality at the M-QTL on different chromosomes;2)introgressing favorable alleles for GYP and grain quality into the parents and then pyramiding and fixing these additive effects in hybrids;and 3)pyramiding overdominant and dominant loci and minimizing or eliminating underdominant loci from the parents. | Hui You Sundus Zafar Fan Zhang Shuangbing Zhu Kai Chen Congcong Shen Xiuqin Zhao Wenzhong Zhang Jianlong Xu | 2022 | The Crop Journal2022，10，6： | 1 |
| 10 | The Formation and Evolution of Uvarovite in UHP Serpentinite and Rodingite and its Constraints on Chromium Mobility in the Oceanic Subduction Zone显示文摘The uvarovite-andradite and uvarovite-andradite-grossular solid-solution series are rare in nature.The discovery of uvarovite-andradite in serpentinite and rodingite from the ultra-high pressure(UHP)metamorphic belt in southwestern Tianshan provided an opportunity to investigate its behavior in the subduction zone.Uvarovite(defined as chromiumgarnet)from serpentinite is homogeneous in a single grain,covering compositions in the uvarovite-andradite solid solution series of Adr_(58-66)Uv_(33-41),with few grossular components.Uvarovite from rodingites contain various Cr_(2)O_(3) contents(1.7-17.9 wt%)and mineral compositions being in the range of Adr_(21-31)Uv_(41-50)Grs_(22-37),Adr_(52-90)Uv_(5-25)Grs_(0-21) and Adr_(19-67)Uv_(3-63)Grs_(13-42).Discontinuous chemical variation of uvarovite from core to rim indicates that uvarovite formed by consuming andradite and chromite,which could provide Ca,Cr,Al and Fe.Raman signals of water were identified for uvarovite from both serpentinite and rodingite,with high water content in uvarovite from serpentinite.The high pressure mineral assemblage,as well as the association with perovskite,indicated that the studied uvarovite from serpentinite and rodingite was formed through high pressure metamorphism,during the subduction zone serpentinization and rodingitization.High alkaline and highly reduced fluids released from serpentinization or rodingitization in the oceanic subduction zone promote the mobility of chromium and enable its long-distance migration. | ZHANG Cong SHEN Tingting ZHANG Lifei LIN Congcong ZHANG Zhongwei Qin Xueqing HU Han QIU Tian XIANG Zhenqun ZHANG Jianxin | 2021 | Acta Geologica Sinica(English Edition)2021，95，5： | 1 |
| 11 | Identification of A-to-I RNA editing profiles and their clinical relevance in lung adenocarcinoma显示文摘Adenosine-to-inosine(A-to-I)RNA editing is a widespread posttranscriptional modification that has been shown to play an important role in tumorigenesis.Here,we evaluated a total of 19,316 RNA editing sites in the tissues of 80 lung adenocarcinoma(LUAD)patients from our Nanjing Lung Cancer Cohort(NJLCC)and 486 LUAD patients from the TCGA database.The global RNA editing level was significantly increased in tumor tissues and was highly heterogeneous across patients.The high RNA editing level in tumors was attributed to both RNA(ADAR1 expression)and DNA alterations(mutation load).Consensus clustering on RNA editing sites revealed a new molecular subtype(EC3)that was associated with the poorest prognosis of LUAD patients.Importantly,the new classification was independent of classic molecular subtypes based on gene expression or DNA methylation.We further proposed a simplified model including eight RNA editing sites to accurately distinguish the EC3 subtype in our patients.The model was further validated in the TCGA dataset and had an area under the curve(AUC)of the receiver operating characteristic curve of 0.93(95%CI:0.91-0.95).In addition,we found that LUAD cell lines with the EC3 subtype were sensitive to four chemotherapy drugs.These findings highlighted the importance of RNA editing events in the tumorigenesis of LUAD and provided insight into the application of RNA editing in the molecular subtyping and clinical treatment of cancer. | Cheng Wang Mingtao Huang Congcong Chen Yuancheng Li Na Qin Zijian Ma Jingyi Fan Linnan Gong Hui Zeng Liu Yang Xianfeng Xu Jun Zhou Juncheng Dai Guangfu Jin Zhibin Hu Hongxia Ma Fengwei Tan Hongbing Shen | 2022 | Science China(Life Sciences)2022，65，1： | 1 |
| 12 | Soil pH drives the spatial distribution of bacterial communities along elevation on Changbai Mountain显示文摘 | Congcong Shen Jinbo Xiong Huayong Zhang Youzhi Feng Xiangui Lin Xinyu Li Wenju Liang Haiyan Chu | 2013 | Soil Biology and Biochemistry2013，，： | 1 |
| 13 | The E3 ubiquitin ligase OsRGLG5 targeted by the Magnaporthe oryzae effector AvrPi9 confers basal resistance against rice blast显示文摘Rice blast,caused by Magnaporthe oryzae,is one of the most devastating diseases of rice.During infection,M.oryzae secretes effectors to facilitate blast development.Among these effectors,the avirulence factor AvrPi9 is recognized by Pi9,a broad-spectrum blast resistance protein that triggers Pi9-mediated resistance in rice.However,little is known about the interaction between AvrPi9 and Pi9 and how AvrPi9 exerts virulence to promote infection.In this study,we found that ectopic expression of AvrPi9 in the Pi9-lacking cultivar TP309 suppressed basal resistance against M.oryzae.Furthermore,we identified an AvrPi9-interacting protein in rice,which we named OsRGLG5,encoding a functional RING-type E3 ubiquitin ligase.During infection,AvrPi9 was ubiquitinated and degraded by OsRGLG5.Meanwhile,AvrPi9 affected the stability of OsRGLG5.Infection assays revealed that OsRGLG5 is a positive regulator of basal resistance against M.oryzae,but it is not essential for Pi9-mediated blast resistance in rice.In conclusion,our results revealed that OsRGLG5 is targeted by the M.oryzae effector AvrPi9 and positively regulates basal resistance against rice blast. | Zhiquan Liu Jiehua Qiu Zhenan Shen Congcong Wang Nan Jiang Huanbin Shi Yanjun Kou | 2023 | Plant Communications2023，4，5： | 1 |
| 14 | Observed characteristics of tidal currents and mean flow in the northern Yellow Sea显示文摘Several bottom-mounted Acoustic Doppler Current Profiler (ADCP) moorings were deployed in the northern Yellow Sea (NYS) during the four seasons of 2006–2007 and also the summertime of 2009. A synthesis analysis on the time-continuous records was performed to examine the characteristics and variations of tidal currents and mean flow over the observation period at these stations. Tidal currents accounted for ~75% of the total kinetic energy, with the absolute dominance of M2 constituent. Visible vertical variations of tidal flow were found on all sites, featured by the decrease of amplitude, increase of rotation rate as well as a decreasing trend of the phase for M2 component with depth. A notable exception was in the central NYS, where the maximum tidal currents occurred in the upper or middle layers (~20–40 m) instead of near the surface (<10 m). The observed mean flow was relatively weak, smaller than 15 cm/s. Velocity on the northern end of Yellow Sea Trough (YST) was characterized by low magnitude and an obvious layered structure vertically. In the Bohai Strait (BS) and the northern slope area, the currents weakened and the flow direction presented a major trend to deflect counterclockwise with depth in most observations. Summertime cyclonic circulation around the Yellow Sea Cold Water Mass (YSCWM), its intensification on the frontal zone and the Yellow Sea Warm Current (YSWC) for the winter season were all evident by our direct current measurements. However, the details of water exchange through the BS appeared partly diff erent from the traditionally-accepted pattern. The vertical diff erences of tidal and mean flow were larger in summer than that in winter, implying the influence of thermal structure to the local currents. Aff ected by the water stratification, mean flow usually reached its maximum near the thermocline in spring and summer, while showing a nearly uniform vertical distribution during winter. | BI Congcong BAO Xianwen DING Yang ZHANG Cong WANG Yu SHEN Biao WAN Kai | 2019 | Journal of Oceanology and Limnology2019，37，2： | 1 |
| 15 | Effects of Low Temperature Stress on the Morphology and hsp70 and hsp90 Gene Expression of Phascolosoma esculenta显示文摘Phascolosoma esculenta is an intertidal organism that has recently attracted attention because of its ability to survive at relatively low temperatures.However,the gene regulation in P.esculenta in relation to its response to low temperatures is unclear.To explore the low temperature adaptability of P.esculenta,this study analyzed the changes in the morphology and hsp70 and hsp90 gene expression of P.esculenta exposed to a low temperature gradient.At 5℃,P.esculenta stretched and softened,and some individuals moved apart from the group.Histological analysis revealed cuticle breaches,myofiber scattering,disruption of the body wall,and epithelial layer dispersion and muscle fiber rupturing in the nephridium.Furthermore,the mRNA expression levels of hsp70 and hsp90 increased under acute low temperature stress,suggesting that these genes function in low temperature tolerance.Overall,low temperature stress causes morphological changes and histological damage in P.esculenta,and hsp70 and hsp90 potentially function in the low temperature adaptability of P.esculenta.Our results provide new insights into the adaptive strategies of P.esculenta under low temperature environments. | SHEN Weiliang LIU Cheng NI Jie GAO Xinming NI Junjie WANG Jianping JIN Shan HOU Congcong WU Xiongfei ZHU Junquan | 2021 | Journal of Ocean University of China2021，20，1： | 0 |
| 16 | Mechanical stretching boosts expansion and regeneration of intestinal organoids through fueling stem cell self-renewal显示文摘Intestinal organoids,derived from intestinal stem cell self-organization,recapitulate the tissue structures and behav-iors of the intestinal epithelium,which hold great potential for the study of developmental biology,disease modeling,and regenerative medicine.The intestinal epithelium is exposed to dynamic mechanical forces which exert profound effects on gut development.However,the conventional intestinal organoid culture system neglects the key role of mechanical microenvironments but relies solely on biological factors.Here,we show that adding cyclic stretch to intestinal organoid cultures remarkably up-regulates the signature gene expression and proliferation of intestinal stem cells.Furthermore,mechanical stretching stimulates the expansion of SOX9+progenitors by activating the Wnt/β-Catenin signaling.These data demonstrate that the incorporation of mechanical stretch boosts the stemness of intestinal stem cells,thus benefiting organoid growth.Our findings have provided a way to optimize an organoid generation system through understanding cross-talk between biological and mechanical factors,paving the way for the application of mechanical forces in organoid-based models. | Fanlu Meng Congcong Shen Li Yang Chao Ni Jianyong Huang Kaijun Lin Zanxia Cao Shicai Xu Wanling Cui Xiaoxin Wang Bailing Zhou Chunyang Xiong Jihua Wang Bing Zhao | 2022 | Cell Regeneration2022，11，1： | 0 |
| 17 | The proximity between hydroxyl and single atom determines the catalytic reactivity of Rh1/CeO_(2) single-atom catalysts显示文摘The local structure of the metal single-atom site is closely related to the catalytic activity of metal single-atom catalysts(SACs).However,constructing SACs with homogeneous metal active sites is a challenge due to the surface heterogeneity of the conventional support.Herein,we prepared two Rh1/CeO_(2)SACs(0.5Rh1/r-CeO_(2)and 0.5Rh1/c-CeO_(2),respectively)using two shaped CeO_(2)(rod and cube)exposing different facets,i.e.,CeO_(2)(111)and CeO_(2)(100).In CO oxidation reaction,the T100 of 0.5Rh1/r-CeO_(2)SACs is 120°C,while the T100 of 0.5Rh1/c-CeO_(2)SACs is as high as 200°C.Via in-situ CO diffuse reflectance infrared Fourier transform spectroscopy(CO-DRIFTS),we found that the proximity between OH group and Rh single atom on the plane surface plays an important role in the catalytic activity of Rh1/CeO_(2)SAC system in CO oxidation.The Rh single atom trapped at the CeO_(2)(111)crystal surface forms the Rh1(OH)adjacent species,which is not found on the CeO_(2)(100)crystal surface at room temperature.Furthermore,during CO oxidation,the OH group far from Rh single atom on the 0.5Rh1/c-CeO_(2)disappears and forms Rh1(OH)adjacent species when the temperature is above 150°C.The formation of Rh1(OH)adjacentCO intermediate in the reaction is pivotal for the excellent catalytic activity,which explains the difference in the catalytic activity of Rh single atoms on two different CeO_(2)planes.The formed Rh1(OH)adjacent-O-Ce structure exhibits good stability in the reducing atmosphere,maintaining the Rh atomic dispersion after CO oxidation even when pre-reduced at high temperature of 500°C.Density functional theory(DFT)calculations validate the unique activity and reaction path of the intermediate Rh1(OH)adjacentCO species formed.This work demonstrates that the proximity between metal single atom and hydroxyl can determine the formation of active intermediates to affect the catalytic performances in catalysis. | Danfeng Wu Shuyun Zhou Congcong Du Juan Li Jianyu Huang Hong-xia Shen Abhaya K.Datye Shan Jiang Jeffrey T.Miller Sen Lin Haifeng Xiong | 2024 | Nano Research2024，17，1： | 0 |
| 18 | A novel Effective Panicle Number per Plant 4 haplotype enhances grain yield by coordinating panicle number and grain number in rice显示文摘Increasing effective panicle number per plant(EPN)is one approach to increase yield potential in rice.However,molecular mechanisms underlying EPN remain unclear.In this study,we integrated mapbased cloning and genome-wide association analysis to identify the EPN4 gene,which is allelic to NARROW LEAF1(NAL1).Overexpression lines containing the Teqing allele(TQ)of EPN4 had significantly increased EPN.NIL-EPN4^(TQ) in japonica(geng)cultivar Lemont(LT)exhibited significantly improved EPN but decreased grain number and flag leaf size relative to LT.Haplotype analysis indicated that accessions with EPN4-1 had medium EPN,medium grain number,and medium grain weight,but had the highest grain yield among seven haplotypes,indicating that EPN4-1 is an elite haplotype of EPN4 for positive coordination of the three components of grain yield.Furthermore,accessions carrying the combination of EPN4-1 and haplotype GNP1-6 of GNP1 for grain number per panicle showed higher grain yield than those with other allele combinations.Therefore,pyramiding of EPN4-1 and GNP1-6 could be a preferred approach to obtain high yield potential in breeding. | Yun Wang Xiaoqian Wang Laiyuan Zhai Sundus Zafar Congcong Shen Shuangbing Zhu Kai Chen Yun Wang Jianlong Xu | 2024 | The Crop Journal2024，12，1： | 0 |
| 19 | Polypyrrole Hollow Nanotubes Loaded with Au and Fe_(3)O_(4) Nanoparticles for Simultaneous Determination of Ascorbic Acid,Dopamine,and Uric Acid显示文摘An ultrasensitive electrochemical biosensor was fabricated for electroanalytical determination of ascorbic acid(AA),dopamine(DA)and uric acid(UA)individually and simultaneously based on polypyrrole hollow nanotubes loaded with Au and Fe_(3)O_(4) nanoparticles(NPs)uniformly(PPy@Au-Fe_(3)O_(4)).The PPy@Au-Fe_(3)O_(4) nanotubes were synthesized in one-pot using MoO_(3) nanorods as templates and the polymerization of Py,the formation of Au and Fe_(3)O_(4) NPs and the removel of MoO_(3) templates took place stimultaneously.Electrochemical studies reveal that PPy@Au-Fe_(3)O_(4) modified glassy carbon electrode(GCE)possesses excellent electrocatalytic activities toward the oxidation of AA,DA and UA.Their oxidation peak currents increase linearly in the concentration ranges of 1–2000µmol/L for AA,0.01–25 and 25–300µmol/L for DA and 0.1–300µmol/L for UA.Their detection limit values(S/N=3)were calculated as 0.45,0.0049,and 0.051µmol/L for AA,DA and UA in the individual detection.By changing the concentrations simultaneously,the calibration curves showed linearity to 1000,200,and 200µmol/L with detection limit of 0.39,0.0060,and 0.060µmol/L for AA,DA,and UA,respectively.Finally,the obtained biosensor was successfully applied to the detection of AA,DA,and UA with satisfactory results on actual samples. | SHEN Congcong CHEN Yuehua FENG Beidou CHI Hongying ZHANG Hua | 2022 | Chemical Research in Chinese Universities2022，38，4： | 0 |
| 20 | Environmental hypoxia induces apoptosis in large yellow croaker Larimichthys crocea via both intrinsic and extrinsic pathways显示文摘Hypoxia has become an unfavorable factor affecting the sustainable development of the large yellow croaker Larimichthys crocea,an economically important mariculture fish in China.Apoptosis is a consequence of hypoxia on fish.However,the effects of hypoxia stress on apoptosis in L.crocea remain largely unknown.We investigated the effect of environmental hypoxia on apoptosis in L.crocea.Results show that hypoxia induced apoptosis in L.crocea both in vivo and in vitro.The mitochondrial membrane potential was significantly reduced in large yellow croaker fry(LYCF)cells.The expression levels of Bcell lymphoma/leukemia-2(Bcl-2)m RNA and protein were also significantly decreased in the liver and LYCF cells during 96 h and 48 h of hypoxia stress,respectively,whereas the expression level of Bcl-2 associated X(Bax)mRNA,Casp3 mRNA,and activity of caspase-3/7/9 were significantly increased,indicating that hypoxia induced caspase-dependent intrinsic apoptosis in L.crocea.The expression level of the apoptosis-inducing factor(AIF)protein was significantly increased in the liver and LYCF cells.The level of AIF protein was significantly decreased in the cytoplasm but increased in the nuclei of L.crocea,demonstrating that hypoxia induced the AIF-mediated caspase-independent intrinsic apoptosis.In addition,the activity of caspase-8 was significantly increased,indicating that hypoxia stress induced extrinsic apoptosis in L.crocea.Therefore,hypoxia induced apoptosis in L.crocea through both the intrinsic and extrinsic pathways.The present study accumulated basic biological information to help elucidate the mechanism of hypoxia response in marine fish. | Shengyu LUO Cheng LIU Xinming GAO Jingqian WANG Yibo ZHANG Jie DING Congcong HOU Junquan ZHU Bao LOU Weiliang SHEN Xiongfei WU Chundan ZHANG | 2023 | Journal of Oceanology and Limnology2023，41，6： | 0 |
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