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| 1 | Green synthesis of gold nanoclusters using papayajuice for detection of L-lysine显示文摘Gold nanoclusters were rapid synthesized within 3 min at 120 ℃ by using papaya juice as a capping and reducing agent(P-AuNCs). The properties of the fluorescent probe were characterized by fluorescent spectroscopy, UV-vis spectroscopy, dynamic light scattering and transmission electron microscope.Based on the surface electron density increase-induced fluorescence enhancing principle, a high selective method for detection of L-lysine was developed with the as-prepared P-AuNCs coupling the fluorescence emission at 440 nm. The fluorescent probe showed high stability and good biocompatibility. Its fluorescence intensity was found to be linearly dependent on the L-lysine concentration in the range of 10.0μmol/L to 1000.0 μmol/L(R^2=0.969) with a limit of detection of 6.0μmol/L. Furthermore, the PAuNCs based approach was applied for monitoring the urine L-lysine contents, demonstrating great potential of fluorescent probes in real samples analysis. | Tian Yu Chengnan Xu Juan Qiao Rongyue Zhang Li Qi | 2019 | Chinese Chemical Letters2019，30，3： | 2 |
| 2 | Recent advances in hydrogel-based anti-infective coatings显示文摘Bacterial infections associated with biomedical devices and implants have posed a great challenge to global healthcare systems.These infections are mainly caused by bacterial biofilm formed on the surface of biomaterials,protecting the encapsulated bacteria from conventional antibiotic treatment and attack of the immune system.As the bacterial biofilm is difficult to eradicate,bactericidal and antifouling coatings have emerged as promising strategies to prevent biofilm fo rmation and subsequent infections.Hydrogels with three-dimensional crosslinked hydrophilic networks,tunable mechanical property and large drugloading capacity are desirable coating materials,which can kill bacteria and/or prevent bacterial adhesion on the surface,inhibiting biofilm formation.Herein,we review recent developments of hydrogels as anti-infective coatings.Particularly,we highlight two chemical approaches(graft-from and graft-to),which have been used to immobilize hydrogels on surfaces,and present advances in the development of bactericidal(contact-killing and antimicrobial-releasing),antifouling(hydrophilic polymer network)and bifunctional hydrogel coatings with both bactericidal and antifouling activities.In addition,the challe nges of hydrogel coatings for clinical applications are discussed,and future research directions of anti-infective hydrogel coatings are proposed. | Mengjing Fu Yijing Liang Xue Lv Chengnan Li Yi Yan Yang Peiyan Yuan Xin Ding | 2021 | Journal of Materials Science & Technology2021，，26： | 1 |
| 3 | Long-term live-cell microscopy with labeled nanobodies delivered by laser-induced photoporation显示文摘Fluorescence microscopy is the method of choice for studying intracellular dynamics.However,its success depends on the.availability of specific and stable markers.A prominent example of markers that are rapidly gaining interest are nanobodies(Nbs.-15 kDa),which can be functionalized with bright and photostable organic fluorophores.Due to their relatively small size and high specificity,Nbs offer great potential for high-quality long-term subcellular imaging,but suffer from the fact that they cannot spontaneously cross the plasma membrane of live cells.We have recently discovered that laser-induced photoporation is well suited to deliver extrinsic labels to living cells without compromising their viability.Being a laser-based technology,it is readily compatible with light microscopy and the typical cell recipients used for that.Spurred by these promising initial results,we demonstrate here for the first time successful long-term imaging of specific subcellular structures with labeled nanobodies in living cells.We illustrate this using Nbs that target GFP/YFP-protein constructs accessible in the cytoplasm,actin-bundling protein Fascin,and the histone H2A/H2B heterodimers.With an efficiency of more than 80%labeled cells and minimal toxicity(-2%),photoporation proved to be an excellent intracellular delivery method for Nbs.Time-lapse microscopy revealed that cell division rate and migration remained unaffected,confirming excellent cell viability and functionality.We conclude that laser-induced photoporation labeled Nbs can be easily delivered into living cells,laying the foundation for further development of a broad range of Nbs with intracellular targets as a toolbox for long-term live-cell microscopy. | Jing Liu Tim Hebbrecht Toon Brans Eef Parthoens Saskia Lippens Chengnan Li Herlinde De Keersmaecker Winnok H.De Vos Stefaan C.De Smedt Rabah Boukherroub Jan Gettemans Ranhua Xiong Kevin Braeckmans | 2020 | Nano Research2020，13，2： | 0 |
| 4 | The Anzhen Risk Scoring System for Acute Type A Aortic Dissection:A Prospective Observational Study Protocol显示文摘Introduction:Acute type A aortic dissection(ATAAD)is a catastrophic disease with fatal outcomes.Malperfusion syndrome(MPS)is a serious complication of ATAAD,with an incidence of 20–40%.Many studies have shown that MPS is the main risk factor for poor ATAAD prognosis.However,a risk scoring system for ATAAD based on MPS is lacking.Here,we designed a risk scoring system for ATAAD to assess mortality through quantitative assessment of relevant organ malperfusion and subsequently develop rational treatment strategies.Methods and analysis:This was a prospective observational study.Patients’perioperative clinical data were col-lected to establish a database of ATAAD(N≥3000)and determine whether these patients had malperfusion complica-tions.The Anzhen risk scoring system was established on the basis of organ malperfusion by using a random forest survival model and a logistics model.The better method was then chosen to establish a revised risk scoring system.Ethics and dissemination:This study received ethical approval from the Ethics Committees of Beijing Anzhen Hospital,Capital Medical University(KS2019034-1).Patient consent was waived because biological samples were not collected,and no patient rights were violated.Findings will be disseminated at scientific conferences and in peer-reviewed publications. | Bo Jia Cheng Luo Chengnan Li Yipeng Ge Yongliang Zhong Zhiyu Qiao Haiou Hu Suwei Chen Junming Zhu | 2023 | Cardiovascular Innovations and Applications2023，7，1： | 0 |
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