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| 1 | Study of the mechanisms of acupuncture and moxibustion treatmentfor ulcerative colitis ratsinview of the gene expression of cytokines显示文摘AIM To observe the effect of acupuncture and moxibustion on the expression of IL 1β and IL 6 mRNA in ulcerative colitis rats. METHODS The SD rat ulcerative colitis model was created by immunological method associated with local stimulation. Colonic mucosa was prepared from human fresh surgical colonic specimens, homogenized by adding appropriate amount of normal saline and centrifuged at 3000*!r/*!min . The supernatant was collected for measurement of protein conentration and then mixed with Freund adjuvant. This antigen fluid was first injected into the plantae of the model group rats, and then into their plantae, dorsa, inguina and abdominal cavities (no Freund adjuvant for the last injection) again on the 10th, 17th, 24th and 31st day. When a certain titer of serum anti colonic antibody was reached, 2% formalin and antigen fluid (no Freund adjuvant) were administered separately by enema. The ulcerative colitis rat model was thus set up. The animals were randomly divided into four groups: model control group (MC, n =8), electro acupuncture group (EA, n =8), herbs partition moxibustion group (HPM 8), normal control group (NC, n =8). HPM: Moxa cones made of refined mugwort floss were placed on the medicinal pad (medicinal pad dispensing: Radix Aconiti praeparata, cortex Cinnamomi, etc ) for Qihai (RN 6) and Tianshu (ST 25, bilateral) and ignited. Two moxa cones were used for each acupoint once a day and 14 times in all. EA: Tianshu (bilateral) and Qihai were stimulated by the intermittent pulse with 2Hz frequency, 4mA intensity for 20 minutes once a day and 14 times in all. After treatment, rats of all four groups were killed simultaneously. The spleen was separated and the distal colon was dissected. Total tissue RNA was isolated by the guanidinium thiocyanate phenol chloroform extraction method. RT PCR technique was used to study the expression of IL 1β and IL 6 mRNA. RESULTS IL 1β and IL 6 mRNAs were not detected in the spleen and colonic mucosa of the NC rats, whereas they were significantly expressed in that of the MC rats. IL 1β and IL 6 mRNAs were markedly lower in the EA and HPM rats than that in MC rats. There was no significant difference between the levels of IL 1β and IL 6 mRNAs in the EA and HPM rats. The expressions of IL 1β and IL 6 mRNAs were nearly the same in the spleen and colon of all groups. CONCLUSION Acupuncture and moxibustion greatly inhibited the expression of IL 1β and IL 6 mRNA in the experimental ulcerative colitis rats. | Wu HG Zhou LB Pan YY Huang C Chen HP Shi Z Hua XG | 1999 | World Journal of Gastroenterology1999，5，6： | 45 |
| 2 | THE CHEMICAL STRUCTURE OF CELASTRINE FROM CELASTRUS FLAGELLARIS显示文摘THECHEMICALSTRUCTUREOFCELASTRINEFROMCELASTRUSFLAGELLARIS￥MingAnWANG;MinWANG;JunLIU;FuHengCHEN(DepatmetofAppliedChemistry,Beij... | Wang, MA Wang, M Liu, J Chen, FH Hong, SL Ma, LB | 1995 | Chinese Chemical Letters1995，6，8： | 3 |
| 3 | Effect of berber- ine on the differentiation of adipocyte 显示文摘 | ZHOU LB CHEN MD WANG X | 2003 | Zhonghua Yi Xue Za Zhi2003，83，4： | 1 |
| 4 | Entrepreneurial Orien- tation, Organizational Learning, and Performance: Evi- dence From China显示文摘 | Zhao YB Li Y Lee SH Chen LB | 2011 | Entrepreneurship Theory and Prac- tice2011，35，2： | 1 |
| 5 | The generation and inactivation mechanism of oxidation-reduction potential of electrolyzed oxidizing water显示文摘 | Liao LB Chen WM Xiao XM | 2007 | Journal of Food Engineering2007，78，： | 1 |
| 6 | Three-dimensional porous graphene-based composite materials : electrochemical synthesis and application 显示文摘 | Chen KW Chen LB Chen YQ | 2012 | J Mater Chem2012，22，20： | 1 |
| 7 | Hypoxia-inducible factor- 1 IX promotes nonhypoxia-mediated proliferation in colon cancer cells and xenografts 显示文摘 | Dang DT Chen F Gardner LB | 2006 | Cancer Res2006，66，3： | 1 |
| 8 | Aberrant promoter methylation of FBLN-3 gene and clinicopathological significance in non-small cell lung carcinoma 显示文摘 | Wang R Zhang YW Chen LB | 2010 | Lung Cancer2010，69，23： | 1 |
| 9 | Multiple molecular chaperones com-plex with misfolded large oligomeric glycoproteins in the endoplasmicreticulum显示文摘 | Kuznetsov G Nigam SK Chen LB | 1997 | J Biol Chem1997，272，： | 1 |
| 10 | IL-22 in the endometriotic milieu promotes the proliferation of endometrial stromal cells via stimu- lating the secretion of CCL2 and IL-8 显示文摘 | Guo Y Chen Y Liu LB | 2013 | Int J Clin Exp Pathol2013，6，： | 1 |
| 11 | HIF-1-αand survivin involved in the anti-apoptotic effect of 2ME2 after global ischemia in rats显示文摘 | Li Y Xia ZL Chen LB | 2011 | Neurol Res2011，33，6： | 1 |
| 12 | Prognostic factors in the acute respiratory distress syndrome显示文摘 | Chen W Ware LB | 2015 | Clin Transla Med2015，4，1： | 1 |
| 13 | Periodontal regeneration using ex vivo autologous stem cells engineered to express the BMP -2 gene : an alternative to alveolaplasty 显示文摘 | Chen YL Chen PK Jeng LB | 2008 | Gene Ther2008，15，22： | 1 |
| 14 | Experience of intrahepatic cholangiocarcinoma in Taiwang显示文摘 | Jan Y Jeng LB | 1999 | J Hep Bil Pancr1999，6，2： | 1 |
| 15 | The generation and inactivation mechanism of oxidation-reduction potential of electrolyzed oxidizing water显示文摘 | Chen WM Xiao XM | 2007 | Journal of Food Engineering2007，78，1： | 1 |
| 16 | Intrahepatic cholangiocarcinoma in Taiwan显示文摘 | CHEN M F JAN YY JENG LB | 1999 | Hepatobiliary-Pancreat- Surg1999，6，2： | 1 |
| 17 | The generation and inactivationmechanism of oxidation - reduction potential of electrolyzed oxidi - zing water 显示文摘 | Liao LB Chen WM Xiao XM | 2007 | Journal of Food Engineering2007，78，1： | 1 |
| 18 | From pattern to process: species and functional diversity in fungal endophytes of Abies beshanzuensis 显示文摘 | Yuan ZL Rao LB Chen YC | 2011 | Fungal Biology2011，115，3： | 1 |
| 19 | Expression of pcriostin homologous with an insect cell adhesion molecule as a prognostic marker in non-small cell lung cancers显示文摘 | Sasak ill LoKM Chen LB | 2001 | Jpn J Cancer Res2001，92，4： | 1 |
| 20 | Hypoxia-inducible factor-1 alpha promotes nonhypoxia-mediated proliferation in colon cancer cells and xenografts显示文摘 | Dang DT Chen F Gardner LB | 2006 | Cancer Res2006，66，3： | 1 |
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