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| 1 | Study of the mechanisms of acupuncture and moxibustion treatmentfor ulcerative colitis ratsinview of the gene expression of cytokines显示文摘AIM To observe the effect of acupuncture and moxibustion on the expression of IL 1β and IL 6 mRNA in ulcerative colitis rats. METHODS The SD rat ulcerative colitis model was created by immunological method associated with local stimulation. Colonic mucosa was prepared from human fresh surgical colonic specimens, homogenized by adding appropriate amount of normal saline and centrifuged at 3000*!r/*!min . The supernatant was collected for measurement of protein conentration and then mixed with Freund adjuvant. This antigen fluid was first injected into the plantae of the model group rats, and then into their plantae, dorsa, inguina and abdominal cavities (no Freund adjuvant for the last injection) again on the 10th, 17th, 24th and 31st day. When a certain titer of serum anti colonic antibody was reached, 2% formalin and antigen fluid (no Freund adjuvant) were administered separately by enema. The ulcerative colitis rat model was thus set up. The animals were randomly divided into four groups: model control group (MC, n =8), electro acupuncture group (EA, n =8), herbs partition moxibustion group (HPM 8), normal control group (NC, n =8). HPM: Moxa cones made of refined mugwort floss were placed on the medicinal pad (medicinal pad dispensing: Radix Aconiti praeparata, cortex Cinnamomi, etc ) for Qihai (RN 6) and Tianshu (ST 25, bilateral) and ignited. Two moxa cones were used for each acupoint once a day and 14 times in all. EA: Tianshu (bilateral) and Qihai were stimulated by the intermittent pulse with 2Hz frequency, 4mA intensity for 20 minutes once a day and 14 times in all. After treatment, rats of all four groups were killed simultaneously. The spleen was separated and the distal colon was dissected. Total tissue RNA was isolated by the guanidinium thiocyanate phenol chloroform extraction method. RT PCR technique was used to study the expression of IL 1β and IL 6 mRNA. RESULTS IL 1β and IL 6 mRNAs were not detected in the spleen and colonic mucosa of the NC rats, whereas they were significantly expressed in that of the MC rats. IL 1β and IL 6 mRNAs were markedly lower in the EA and HPM rats than that in MC rats. There was no significant difference between the levels of IL 1β and IL 6 mRNAs in the EA and HPM rats. The expressions of IL 1β and IL 6 mRNAs were nearly the same in the spleen and colon of all groups. CONCLUSION Acupuncture and moxibustion greatly inhibited the expression of IL 1β and IL 6 mRNA in the experimental ulcerative colitis rats. | Wu HG Zhou LB Pan YY Huang C Chen HP Shi Z Hua XG | 1999 | World Journal of Gastroenterology1999，5，6： | 45 |
| 2 | CT arterial portography and CT hepatic arteriography in detection of micro liver cancer显示文摘AIM To recognize the characteristic findings of micrQliver cancer (MLC) and to evaluate the effect of CT arterial portography (CTAP) and CT hepatic arteriography (CTHA) in diagnosis of MLC. METHODS Between April 1996 to December 1998, CTAP and CTHA were performed in 12patients with MLC, which were not detect ed byconventional CT examinations. After CTHA, 3 mL-- 5 mL mixture of lipiodol, doxorubic in andmitomycin C were injected into hepatic arterythrough the catheter, and then followed up by CTthree or four weeks later (Lipiodol CT LP-CT).RESULTS A total of 22 micro--tumors (0 .2 cm 0.6 cm in diameter ) were detected in 12patients, which manifested as small perfusiondefects in CTAP and small round enhancement inCTHA. The rate of detectability of CTAP andCTHA was 68.2% (15/ 22) and 77.3% (17/ 22)respectively, and the rate of the simultaneoususe of both procedures reached 86. 4% (19/ 22 ).All micro--tumors were demonstrated as punctatelipiodol deposit fool in LP--CT. After LP--CT, theelevated serum level of Q-fetoprotein (AFP)dropped to the normal level in all patients.CONCLUSION The CTAP and CTHA are the mostsensitive imaging methods for detecting microIiver cancer. Confirmed by the change of theelevated serum AFP level and lipiodol depositfool in LP-CT, small perfusion defects in CTAPand punctate enhancement in CTHA may suggestmicro--liver cancer. | Li L Wu PH Mo YX Lin HG Zheng L Li JQ Lu LX Ruan CM Chen L | 1999 | World Journal of Gastroenterology1999，5，3： | 16 |
| 3 | Fluorodeoxyglucose positron-emission tomography ratio in non-small cell lung cancer patients treated with definitive radiotherapy 显示文摘 | Kang HC Wu HG Yu T et aI | 2013 | Radiat Oncol J2013，31，3： | 1 |
| 4 | LIN28B confers radio-resistance through the posttranscriptional control of KRAS显示文摘 | Jeong SH Wu HG Park WY | | 0，，12： | 1 |
| 5 | High cyclooxygenase-2 expression is related with distant metastasis in cervical cancer treated with radiotherapy 显示文摘 | KIM H J WU HG PARK IA | 2003 | Int J Radiat Oncel Biol Phys2003，55，1： | 1 |
| 6 | Concurrent radiotherapy with paclitaxel/carboplatin chemotherapy as a definitive treatment for squamous cell carcinoma of the uterine cervix显示文摘 | Lee MY Wu HG Kim K | 2007 | Gynecol Oncol2007，104，1： | 1 |
| 7 | Functional and aesthetic results of immediate Reconstruction of traumatic thumb de- fects by toe - to - thumb transplantation 显示文摘 | Huang D Wang HG Wu WZ | 2011 | Int Orthop2011，35，4： | 1 |
| 8 | Chitooligosaccharides inhibit nitric oxide mediated migration of endothelial cells in vitro and tumor angiogenesis in vitro 显示文摘 | Wu HG Yao ZA Bai XF | 2010 | Carbohydrate Polymers2010，82，3： | 1 |
| 9 | Irinotecan in combination with radiation therapy for small-cell and non-small-cell lung cancer显示文摘 | Wu HG Choy H | 2002 | Oncology(Huntingt)2002，16，9： | 1 |
| 10 | Effect and mechanism of cantharides vesiculation moxibustion on hematopoietic function inmice treated with cyclophosphamide显示文摘 | Sun DL Chen HP Wu HG et a1 | 2011 | J Acupunct Tuina Sci2011，9，6： | 1 |
| 11 | The characteristics of the stone and urine composition in chinese stone fomers:Primary re- port of a single -center results显示文摘 | Wu W Yang D Tiselius HG | 2014 | Urology2014，83，4： | 1 |
| 12 | Concurrent radiotherapy with paelitaxel/carboplatin chemotherapy as a definitive treatment for squamous cell carcinoma of the uterine cervix显示文摘 | LEE MY WU HG KIM K | 2007 | Gynecol Oncol2007，104，1： | 1 |
| 13 | Concurrent radiotherapy with paclitaxel/carboplatin chemotherapy as a definitive treatment for squamous cell carcinoma of the uterine cervix显示文摘 | Lee MY Wu HG Kim K | 2007 | Gynecol Oncol2007，104，1： | 1 |
| 14 | Comparison of the efficacy between paclitaxel/carboplatin and doxorubicin/cisplatin for concurrent chemoradiation in intermediate- or high-risk endometrioid endometrial cancer: a single institution experience 显示文摘 | Kim HS Kim JW Wu HG | 2010 | J Obstet Gynaecol Res2010，36，3： | 1 |
| 15 | Adjuvant single-fraction radiothera- py is safe and effective for intractable keloids显示文摘 | Song C Wu HG Chang H | 2014 | Radiat Res2014，55，5： | 1 |
| 16 | Efficacy of paclitaxel and carboplatin as a regimen for postoperative concurrent chemoradiotherapy of high risk uterine cervix cancer显示文摘 | Kim K Chie EK Wu HG | 2006 | Gynecol Oncol2006，101，3： | 1 |
| 17 | Treatment outcomes and prognostic fac-tors in cervical cancer patients treated with postoperative ex-tended field radiation therapy 显示文摘 | Kim HJ Ha SW Wu HG | 2009 | J Gynecol Oncol2009，20，4： | 1 |
| 18 | Electro acupuncture and moxibustion promote neutrophil apoptosis and inprove ulcerative colitis in rats 显示文摘 | Wu HG Liu HR Tan LY | 2007 | Dig Dis Sci2007，52，2： | 1 |
| 19 | Therapeutical effect of recombinant human epidermal growth factor(RhEGF)on mucositisin patients undergoing radiotherapy,with or without chemotherapy,for head and neck cancer:a double blind place controlled prospective phase 2 multiinstitutional clinical trial显示文摘 | Wu HG Song SY Kim YS | 2009 | Cancer2009，115，12： | 1 |
| 20 | Regulatory mechanism of electro acupuncture in irritable bowel syndrome: preventing MC activation and decreasing SP, VIP secretion显示文摘 | Wu HG Jiang B Zhou EH | 2008 | Dig Dis Sci2008，55，6： | 1 |
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