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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | In vivo theranostics with near-infraredemitting carbon dots—highly efficient photothermal therapy based on passive targeting after intravenous administration显示文摘Carbon dots that exhibit near-infrared fluorescence(NIR CDs)are considered emerging nanomaterials for advanced biomedical applications with low toxicity and superior photostability and targeting compared to currently used photoluminescence agents.Despite progress in the synthesis of NIR CDs,there remains a key obstacle to using them as an in vivo theranostic agent.This work demonstrates that the newly developed sulfur and nitrogen codoped NIR CDs are highly efficient in photothermal therapy(PTT)in mouse models(conversion efficiency of 59%)and can be readily visualized by photoluminescence and photoacoustic imaging.The real theranostic potential of NIR CDs is enhanced by their unique biodistribution and targeting.Contrary to all other nanomaterials that have been tested in biomedicine,they are excreted through the body’s renal filtration system.Moreover,after intravenous injection,NIR CDs are accumulated in tumor tissue via passive targeting,without any active species such as antibodies.Due to their accumulation in tumor tissue without the need for intratumor injection,high photothermal conversion,excellent optical and photoacoustic imaging performance,and renal excretion,the developed CDs are suitable for transfer to clinical biomedical practice. | Xin Bao Ye Yuan Jingqin Chen Bohan Zhang Di Li Ding Zhou Pengtao Jing Guiying Xu Yingli Wang Kateřina Holá Dezhen Shen Changfeng Wu Liang Song Chengbo Liu Radek Zbořil Songnan Qu | 2018 | Light(Science & Applications)2018，7，1： | 13 |
| 3 | Surface integrity and fatigue behavior when turning γ-TiAl alloy with optimized PVD-coated carbide inserts显示文摘This paper presents a comprehensive investigation on the effects of tool and turning parameters on surface integrity and fatigue behavior in turning c-Ti Al alloy. The wear of inserts surface, cutting forces, and surface roughness were studied to optimize PVD-coated carbide inserts.Surface topography, residual stresses, microhardness, and microstructure were analyzed to characterize the surfaces layer under different turning parameters. Surface integrity and fatigue life tests of c-Ti Al alloy were conducted under turning and turning-polishing processes. The results show that compared to CNMG120412-MF4, CNMG120408-SM is more suitable because it obtained low cutting force, surface roughness, and tool wear. With increasing the cutting speed and depth, the depths of the compressive residual stress layer, hardening layer, and plastic deformation layer increased. For turning and turning-polishing specimens, the compressive residual stress was relaxed by less than 20%–30% after 10~7 cycles. The fatigue life of a turning-polishing specimen with R_a= 0.15 mm has increased 3 times from that of a turning specimen with R_a= 0.43 mm. | Changfeng YAO Jiannan LIN Daoxia WU Junxue REN | 2018 | Chinese Journal of Aeronautics2018，31，4： | 9 |
| 4 | NIR-IIb excitable bright polymer dots with deep-red emission for in vivo through-skull three-photon fluorescence bioimaging显示文摘It is of great significance to study the brain structure and function in deep-tissue for neuroscience research and bio-medical applications because of the urgent demand for precise theranostics.Three-photon fluorescence microscopic(3PFM)bioimaging excited by the light in near-infrared IIb(NIR-IIb,1,500–1,700 nm)spectral region is one of the most promising imaging techniques with the advantages of high spatial resolution,large imaging depth,and reduced scattering.Herein,a type of NIR-IIb light excitable deep-red emissive semiconducting polymer dots(P-dots)with bright 3PF and large three-photon absorption cross-section(σ3)at 1,550 nm was prepared.Then the P-dots were functionalized with polystyrene polymer polystyrene graft ethylene oxide functionalized with carboxyl groups(PS-PEG-COOH)and modified with NH2-poly(ethylene glycol)(PEG)to synthesis photochemically stable and biocompatible P-dots nanoparticles(NPs).Further the P-dots NPs were utilized for in vivo 3PFM bioimaging of cerebral vasculature with and without the brain skull under 1,550 nm femtosecond(fs)laser excitation.In vivo 3PFM bioimaging of the mice cerebral vasculature at various vertical depths was obtained.Moreover,a vivid three-dimensional structure of the mice vascular architecture beneath the skull was reconstructed.At the depth of 350μm beneath the brain skull,3.8μm blood vessels could still be clearly recognized.NIR-IIb excitable P-dots assisted 3PFM bioimaging has great potential in accurate deep tissue bioimaging. | Nuernisha Alifu Abudureheman Zebibula Hequn Zhang Huwei Ni Liang Zhu Wang Xi Yalun Wang Xueliang Zhang Changfeng Wu Jun Qian | 2020 | Nano Research2020，13，10： | 6 |
| 5 | Isolation via enrichment and characterization of ten polymorphic microsatellite loci in the cuttlefish, Sepiella maindroni de Rochebruns显示文摘Ten novel polymorphic microsatellite loci were developed using an enrichment and colony hybridization protocol and characterized for the cuttlefish,Sepiella maindroni.Polymorphism was explored by using 30 individuals from the coastal waters of Zhanjiang,Guangdong province,China,in December 2007.The number of alleles ranged from 5 to 13.The ranges of observed and expected heterozygosity were from 0.578 9 to 1.000 0 and 0.682 8 to 0.925 7,respectively,and the average polymorphic information content (P IC) was 0.778 5.These microsatellite loci will certainly facilitate the detection of the genetic variation and population structure of S.maindroni. | WU Changwen CHI Changfeng HE Guangyuan LU Zhenming XU Meiying | 2010 | Acta Oceanologica Sinica2010，29，6： | 6 |
| 6 | Indole-3-propionic Acid-aggravated CCl_(4)-induced Liver Fibrosis via the TGF-β1/Smads Signaling Pathway显示文摘Background and Aims:The pathogenesis of liver fibrosis involves liver damage,inflammation,oxidative stress,and intestinal dysfunction.Indole-3-propionic acid(IPA)has been demonstrated to have antioxidant,anti-inflammatory and anticancer activities,and a role in maintaining gut homeostasis.The current study aimed to investigate the role of IPA in carbon tetrachloride(CCl4)-induced liver fibrosis and explore the underlying mechanisms.Methods:The liver fibrosis model was established in male C57BL/6 mice by intraperitoneal injection of CCl4 twice weekly.IPA intervention was made orally(20 mg/kg daily).The degree of liver injury and fibrosis were assessed by serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),and histopathology.Enzyme-linked immunosorbent assay and quantitative real-time polymerase chain reaction(qPCR)were used to detect the inflammatory cytokines.The malondialdehyde(MDA),glutathione,glutathione peroxidase,superoxide dismutase,and catalase were determined via commercial kits.Hepatocyte apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay.The expression of mRNA and protein was assayed by qPCR,Western blotting,or immunohistochemical staining.Results:After IPA treatment,the ALT and AST,apoptotic cells,and pro-inflammatory factor levels were enhanced significantly.Moreover,IPA intervention up-regulated the expression of collagen I,α-smooth muscle actin,tissue inhibitor of matrix metalloproteinase-1,matrix metalloproteinase-2,transforming growth factor-β1(TGF-β1),Smad3,and phosphorylated-Smad2/3.Additionally,IPA intervention did not affect the MDA level.Attractively,the administration of IPA remodeled the gut flora structure.Conclusions:IPA aggravated CCl_(4)-induced liver damage and fibrosis by activating HSCs via the TGF-β1/Smads signaling pathway. | Fei Liu Changfeng Sun Yuanfang Chen Fei Du Yuxiang Yang Gang Wu | 2021 | Journal of Clinical and Translational Hepatology2021，9，6： | 5 |
| 7 | Carboniferous ridge subduction in the Xingmeng Orogenic Belt:Constraints from geochronological,geochemical,and Sr-Nd-Hf isotopic analysis of strongly peraluminous granites and gabbro-diorites in the Xilinhot micro-continent显示文摘The Xingmeng Orogenic Belt evolved through a long-lived orogeny involving multiple episodes of subduction and accretion.However,there is a debate on its tectonic evolution during the Late Paleozoic.Here,we report geochemical,geochronological,and isotopic data from strongly peraluminous granites and gabbro-diorites from the Sunidzuoqi-Xilinhot region.Zircon U-Pb ages suggest that the intrusive rocks were emplaced during the Early Carboniferous(333-322 Ma).The granites exhibit geochemical characteristics similar to S-type granites,with high SiO_(2)(72.34-76.53 wt.%),Al_(2)O_(3)(12.45-14.65 wt.%),and A/CNK(1.07-1.16),but depleted Sr,Nb,and Ta contents.They exhibit positiveε_(Nd)(t)andε_(Hf)(t)values(-0.3 to 2.8 and 2.7-5.7,respectively)and young Nd and Hf model ages(T_(DM2)(Nd)=853-1110 Ma and T_(DM2)(Hf)=975-1184 Ma),suggesting that they may be the partial melting products of heterogeneous sources with variable proportions of pelite,psammite,and metabasaltic rocks.The meta-gabbro-diorites from the Maihantaolegai pluton have low SiO_(2)(47.06-53.49 wt.%)and K_2O(0.04-0.99 wt.%)contents,and demonstrate slight light rare earth element(REE)depletion in the chondritenormalized REE diagrams.They have high zirconε_(Hf)(t)values(14.41-17.34)and young Hf model ages(T_(DM2)(Hf)=230-418 Ma),indicating a more depleted mantle source.The variations of the Sm/Yb and La/Sm ratios can thus be used to assess the melting degree of the mantle source from 5%to 20%,suggesting a quite shallow mantle melting zone.We propose that the petrogenesis and distribution of the strongly peraluminous granites and gabbro-diorites,as well as the tectonic architecture of the region,can be explained by a ridge subduction model.Based on these results,and previous studies,we suggest a southward ridge subduction model for the Sunidzuoqi-Xilinhot region. | Changfeng Liu Zhiguang Zhou Guosheng Wang Chen Wu Hongying Li Shiwei Ma | 2021 | Geoscience Frontiers2021，12，3： | 2 |
| 8 | Photoluminescence from surfactant-assembled Y2O3:Eu nanotubes显示文摘 | Wu Changfeng Qin Weiping Qin Guanshi | 2003 | Appl Phys Lett2003，82，： | 1 |
| 9 | Highly Fluorescent Semiconducting Polymer Dots for Biology and Medicine显示文摘 | Changfeng Wu Daniel T. Chiu | 2013 | Angew. Chem. Int. Ed2013，，11： | 1 |
| 10 | Stable Functionalization of Small Semiconducting Polymer Dots via Covalent Cross‐Linking and Their Application for Specific Cellular Imaging显示文摘 | Jiangbo Yu Changfeng Wu Xuanjun Zhang Fangmao Ye Maria Elena Gallina Yu Rong I‐Che Wu Wei Sun Yang‐Hsiang Chan Daniel T. Chiu | 2012 | Adv. Mater2012，，26： | 1 |
| 11 | Ratiometric Single‐Nanoparticle Oxygen Sensors for Biological Imaging显示文摘 | Changfeng Wu Barbara Bull Kenneth Christensen Jason McNeill | 2009 | Angew. Chem. Int. Ed2009，，15： | 1 |
| 12 | Photoluminescence from surfactant-assembled Y2O3 : Eu nanotubes显示文摘 | WU CHANGFENG QIN WEIPING QIN GUANSHI etal | 2003 | Appl Phys Lett2003，82，4： | 1 |
| 13 | Photoluminescence from suHactant-assembled Y2O3 : Eu nanotubes显示文摘 | WU Changfeng QIN Weiping QIN Guanshi etal | 2003 | Appl Phys Lett2003，82，4： | 1 |
| 14 | Determination of L-Cystine by a New Sensitive Cucurbit[7]uril/palmatine Probe显示文摘面对葫芦[7 ] uril (CB [7 ]) , CB [7 ] 能与巴马亭反应,它用作一根敏感荧光探针,到形式主人客人,稳定的建筑群和建筑群的荧光紧张极大地被提高。荧光紧张在包括系统与越来越多的 L 胱氨酸线性地减少了。试验性的结果证明那在那里存在在为 CB 的 L 胱氨酸和巴马亭之间的一场比赛[7 ] 恐水病的洞和 L 胱氨酸占据 CB 的空间[7 ] 洞,要强迫居住在水的环境的领先的巴马亭分子。基于 CB 熄灭的荧光[7 ]/palmatine 建筑群源于在 CB 之间的复杂形成[7 ] 并且 L 胱氨酸,为在水的答案的 L 胱氨酸的决心的一个 spectrofluorimetric 方法面对 CB [7 ] 被开发。在熄灭 F 和 L 胱氨酸集中的荧光的相应价值之间的线性关系在 6.0 ~ 1.5 漠 ? 桴 ? 楦摤敬的范围被获得吗? | Yang, Jiyuan Du, Liming Wu, Hao Chang, Yinxia Li, Changfeng | 2011 | Chinese Journal of Chemistry2011，29，6： | 1 |
| 15 | Infrared-to-visible upconversion luminescence of Er^3+ and Yb^3+ co-doped germanate glass显示文摘 | Guanshi Qin Weiping Qin Changfeng Wu | 2004 | J Non-Cryst Solids2004，347，： | 1 |
| 16 | Effects of cutting parameters on surface residual stress and its mecha- nism in high-speed milling of TB6显示文摘 | YAO Changfeng WU Daoxia TAN Liang | 2013 | Proceedings of the In- stitution of Mechanical Engineers Part B: Journal of Engi- neering Manufacture2013，227，4： | 1 |
| 17 | IFC model viewer to support nD model application显示文摘 | Changfeng Fu Ghassan Aouad Angela Lee Amanda Mashall-Ponting Song Wu | 2006 | Automation in construction2006，15，： | 1 |
| 18 | Geochemistry of the Mesoproterozoic Intrusions, Geochronology and Isotopic Constraints on the Xiaonanshan Cu-Ni Deposit along the Northern Margin of the North China Craton显示文摘Mesoproterozoic magma events in the Bayan Obo rift belt have remained poorly constrained and as a result, the Late Paleoproterozoic–Mesoproterozoic tectonic evolution of the rift belt has remained unclear. By a multiple-facetted regional geological investigation of this belt, we have resolved the stratigraphic sequence and geochronology of the Bayan Obo Group and made new discoveries including a three-stage mantle-derived magmatic sequence. Zircon and baddeleyite dating of Xiaonanshan hornblende pyroxenite emplaced into the Bayan Obo Group yields 207Pb/206Pb ages of ca. 1.34 and 1.33 Ga. The geochronological, geochemistry, Hf isotopic analyses place an important constraint on ages of the Late Paleoproterozoic–Mesoproterozoic strata and the evolution of the rift belt. Our field observations and U-Pb dating results suggest that mineralization is genetically related to Mesoproterozoic magmatism in North China Craton, i.e., 1.33–1.34 Ga. The δ34SV-CDT values of sulphide from the ore-bearing ultra-/mafic samples are about 6.2‰, whereas the 206Pb/204Pb, 207Pb/204Pb, and 208Pb/204Pb values vary in the ranges of 17.598–18.115, 15.496–15.501, and 37.478–37.952, respectively. The Late Paleozoic mafic gabbro and acidic granite porphyry intrusions are possible to bimodal magmatic event related to the extensional tectonic setting of the Central Asia in this period. | Zhiguang Zhou Jiangwei Yi Niu Guosheng Wang Chen Wu Changfeng Liu Juncheng Ju | 2020 | Journal of Earth Science2020，31，4： | 0 |
| 19 | Pretreatment mechanism ofβ-O-4 lignin during phosphoric acid-acetone process based on density functional theory and molecular dynamic simulations显示文摘Pretreatment mechanism ofβ-O-4 lignin(Lβ-O-4)during the phosphoric acid-acetone process involves a series of interactions between lignin and solvent molecule(H2O,CH3COCH3 and H3PO4)which lead to the adsorption,solubility and decomposition of lignin.Coniferyl alcohol guaiacyl glycerol(CAGG)with the predominant linkage(β-O-4 ether bond)was chosen as the modelβ-O-4 lignin(Lβ-O-4)for investigating the detailed pretreatment mechanism based on density functional theory calculations and molecular dynamic simulations.Interactions betweenβ-O-4 lignin and solvent molecules were firstly detected.Only physical interaction occurred betweenβ-O-4 lignin and the solvent molecule.The attractive van der Waals interaction favored CH3COCH3 molecules approaching to Lβ-O-4,showing a compatibility of Lβ-O-4 in CH3COCH3 solution.Furthermore,following the temperature effect on the dynamics processes,larger dynamics calculations and experiments were carried out to reveal the detailed dissolution and precipitation ofβ-O-4 lignin in various solutions. | Zhang Junjiao Zhu Jinqi Lin Changfeng Wang Tipeng Dong Changqing Qin Wu | 2016 | International Journal of Agricultural and Biological Engineering2016，9，2： | 0 |
| 20 | Erratum to:NIR-IIb excitable bright polymer dots with deep-red emission for in vivo through-skull three-photon fluorescence bioimaging显示文摘Erratum to Nano Research 2020,13(10):2632–2640 http://gffzzd3cc09b8251d45dfs0nk9qfx9xw5k6p6k.ffgz.tsg.suse.edu.cn/10.1007/s12274-020-2902-x Figures S5 and S6 in the Electronic Supplementary material(ESM)were unfortunately mistakenly used.This error did not affect any of the conclusions from the published paper.Instead of Figure S5 Microscopic images of tissue sections from mice. All tissues of (a)heart, (b) lung, (c) liver, (d) spleen, (e) kidney and (f) brain were excised fromthe experimental mice 24 hours after the administration of P-dots (200 μL,0.125 mg·mL^(-1) in 1 × PBS, right) and the control mice (left) treated with 1 × PBS(200 μL). Scale bar: 50 μm. | Nuernisha Alifu Abudureheman Zebibula Hequn Zhang Huwei Ni Liang Zhu Wang Xi Yalun Wang Xueliang Zhang Changfeng Wu Jun Qian | 2022 | Nano Research2022，15，6： | 0 |
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