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| 1 | Clinical-pathological and molecular characterization of long-term survivors with advanced non-small cell lung cancer显示文摘Objective:Long-term survivors(LS)of non-small cell lung cancer(NSCLC)without driver alterations,displaying an overall survival(OS)of more than 3 years,comprise around 10%of cases in several series treated with chemotherapy.There are classical prognosis factors for these cases[stage,Eastern Cooperative Oncology Group(ECOG),etc.],but more data are required in the literature.In this multi-center study,we focused on LS of advanced NSCLC with OS above 36 months to perform a clinical-pathological and molecular characterization.Methods:In the first step,we conducted a clinical-pathological characterization of the patients.Afterwards,we carried out a genetic analysis by comparing LS to a sample of short-term survivors(SS)(with an OS less than 9 months).We initially used whole-genome RNA-seq to identify differentiating profiles of LS and SS,and later confirmed these with reverse transcription-polymerase chain reaction(RT-PCR)for the rest of the samples.Results:A total of 94 patients were included,who were mainly men,former smokers,having adenocarcinoma(AC)-type NSCLC with an ECOG of 0-1.We obtained an initial differential transcriptome expression,displaying 5 over-and 33 under-expressed genes involved in different pathways:namely,the secretin receptor,surfactant protein,trefoil factor 1(T FF1),serpin,Ca-channels,and Tolllike receptor(TLRs)families.Finally,RT-PCR analysis of 40(20 LS/20 SS)samples confirmed that four genes(surfactant proteins and SFTP)were significantly down-regulated in SS compared to LS by using an analysis of covariance(ANCOVA)model:SFTPA1(P=0.023),SFTPA2(P=0.027),SFTPB{P=0.02),and SFT PC(P=0.047).Conclusions:We present a sequential genetic analysis of a sample of NSCLCLS with no driver alterations,obtaining a differential RNA-seq/RT-PCR profile showing an abnormal expression of SF genes. | Juan Moreno-Rubio Santiago Ponce Rosa Alvarez Maria Eugenia Olmedo Sandra Falagan Xabier Mielgo Fatima Navarro Patricia Cruz Luis Cabezon-Gutierrez Carlos Aguado Gonzalo Colmenarejo Marta Munoz-Fernandez de Leglaria Ana Belen Enguita Maria Cebollero Amparo Benito Isabel Alemany Carolina del Castillo Ricardo Ramos Ana Ramirez de Molina Enrique Casado Maria Sereno | 2020 | Cancer Biology & Medicine2020，17，2： | 4 |
| 2 | An elastin-like recombinamer-based bioactive hydrogel embedded with mesenchymal stromal cells as an injectable scaffold for osteochondral repair显示文摘The aim of this study was to evaluate injectable,in situ cross-linkable elastin-like recombinamers(ELRs)for osteochondral repair.Both the ELR-based hydrogel alone and the ELR-based hydrogel embedded with rabbit mesenchymal stromal cells(rMSCs)were tested for the regeneration of critical subchondral defects in 10 New Zealand rabbits.Thus,cylindrical osteochondral defects were filled with an aqueous solution of ELRs and the animals sacrificed at 4months for histological and gross evaluation of features of biomaterial performance,including integration,cellular infiltration,surrounding matrix quality and the new matrix in the defects.Although both approaches helped cartilage regeneration,the results suggest that the specific composition of the rMSC-containing hydrogel permitted adequate bone regeneration,whereas the ELR-based hydrogel alone led to an excellent regeneration of hyaline cartilage.In conclusion,the ELR cross-linker solution can be easily delivered and forms a stable well-integrated hydrogel that supports infiltration and de novo matrix synthesis. | Filippo Cipriani Blanca Arino Palao Israel Gonzalez de Torre Aurelio Vega Castrillo Hector Jose Aguado Hernandez Matilde Alonso Rodrigo Angel Jose Alvarez Barcia Ana Sanchez Veronica Garcıa Diaz Monica Lopez Pena Jose Carlos Rodriguez-Cabello | 2019 | Regenerative Biomaterials2019，6，6： | 3 |
| 3 | 3D additive manufactured composite scaffolds with antibiotic-loaded lamellar fillers for bone infection prevention and tissue regeneration显示文摘Bone infections following open bone fracture or implant surgery remain a challenge in the orthopedics field.In order to avoid high doses of systemic drug administration,optimized local antibiotic release from scaffolds is required.3D additive manufactured(AM)scaffolds made with biodegradable polymers are ideal to support bone healing in non-union scenarios and can be given antimicrobial properties by the incorporation of antibiotics.In this study,ciprofloxacin and gentamicin intercalated in the interlamellar spaces of magnesium aluminum layered double hydroxides(MgAl)andα-zirconium phosphates(ZrP),respectively,are dispersed within a thermoplastic polymer by melt compounding and subsequently processed via high temperature melt extrusion AM(~190◦C)into 3D scaffolds.The inorganic fillers enable a sustained antibiotics release through the polymer matrix,controlled by antibiotics counterions exchange or pH conditions.Importantly,both antibiotics retain their functionality after the manufacturing process at high temperatures,as verified by their activity against both Gram+and Gram-bacterial strains.Moreover,scaffolds loaded with filler-antibiotic do not impair human mesenchymal stromal cells osteogenic differentiation,allowing matrix mineralization and the expression of relevant osteogenic markers.Overall,these results suggest the possibility of fabricating dual functionality 3D scaffolds via high temperature melt extrusion for bone regeneration and infection prevention. | María C´amara-Torres Stacy Duarte Ravi Sinha Ainhoa Egizabal Noelia´Alvarez Maria Bastianini Michele Sisani Paolo Scopece Marco Scatto Alessandro Bonetto Antonio Marcomini Alberto Sanchez Alessandro Patelli Carlos Mota Lorenzo Moroni | 2021 | Bioactive Materials2021，6，4： | 2 |
| 4 | Assessment and simulation of the responsive demand potential in end-user facilities: Application to a university customer显示文摘 | Alvarez Carlos Gabaldon Antonio Molina Angel | 2004 | IEEE Transactions on Power Systems2004，19，2： | 2 |
| 5 | Ileocolic intussusception due to intestinal metastatic melanoma. Case report and review of the literature显示文摘 | Fernando A. Alvarez Matías Nicolás Jeremías Goransky Carlos A. Vaccaro Axel Beskow Demetrio Cavadas | 2011 | International Journal of Surgery Case Reports2011，，6： | 2 |
| 6 | Economic, social and environmental impact of land consolidation in Galicia显示文摘 | Rafael Crecente Carlos Alvarez Urbano Fra | 2002 | Land Use Policy2002，，2： | 2 |
| 7 | Economic, social and environmenlal impact of land consohdation in Galicia 显示文摘 | Rafael Crecente Carlos Alvarez Urbano Fra | 2002 | Land Use Policy2002，2002，： | 1 |
| 8 | The sticholysin family of pore-forming toxins induces the mixing of lipids in membrane domains显示文摘 | Uris Ros Michelle A. Edwards Raquel F. Epand Maria E. Lanio Shirley Schreier Christopher M. Yip Carlos Alvarez Richard M. Epand | 2013 | BBA - Biomembranes2013，，11： | 1 |
| 9 | Micromophological characteristics of a marine ferromanganese crust 显示文摘 | Robustiano Alvarez Eric H De Carlo James Cowen | 1990 | Marine Geology1990，94，： | 1 |
| 10 | Serum Cystatin C as a New Marker for Noninvasive Estimation of Glomerular Filtration Rate and as a Marker for Early Renal Impairment显示文摘 | Elisabeth Coll Albert Botey Luisa Alvarez Esteban Poch Lloren? Quintó Ana Saurina Manel Vera Carlos Piera Alejandro Darnell | 2000 | American Journal of Kidney Diseases2000，，1： | 1 |
| 11 | Design and field test of an automatic data acquisition system in a self-propelled forage harvester显示文摘 | Carlos Amiama Javier Bueno Carlos Jose Alvarez | 2008 | Computers and Electronics in Agriculture2008，16，2： | 1 |
| 12 | Economic social and environmental impact of land consolidation in Galicia显示文摘 | Rafael Crecente Carlos Alvarez | 2002 | Land Use Policy2002，，19： | 1 |
| 13 | Economic, social and environmental impact of land consolidation in Galicia 显示文摘 | Rafael Crecente Carlos Alvarez Urbano Fra | 2002 | Land Use Policy2002，19，2： | 1 |
| 14 | Support vector machines used to estimate the battery state of charge显示文摘 | Juan Carlos Alvarez Anton Paulino Jose Garca Nieto Cecilio Blanco Viejo | 2013 | IEEE Transactions on Power Electronics2013，28，12： | 1 |
| 15 | Determination of the critical product layer thickness in the reaction of CaO with CO2 显示文摘 | Diego Alvarez Carlos Abanades J | 2005 | Ind Eng Chem Res2005，44，15： | 1 |
| 16 | Economic,social and environmental impact of land consolidation in galicia显示文摘 | Rafael Crecente Carlos Alvarez Urbano Fra | 2002 | Land Use Policy2002，，19： | 1 |
| 17 | Pore-size and shape effects on the recarbonation performance of calcium oxide submitted to repeated calcination/recarbonation cycles 显示文摘 | Diego Alvarez Carlos Abanades J | 2005 | Energy & Fuels2005，19，1： | 1 |
| 18 | Pancreatic interstitial pH in human and feline chronic pancreatitis显示文摘 | Ameet G. Patel Mark T. Toyama Carlos Alvarez Thanh N. Nguyen Peter U. Reber Stanley W. Ashley Howard A. Reber | 1995 | Gastroenterology1995，，5： | 1 |
| 19 | Pathophysiologic mechanisms of cardiovascular disease inobstructive sleep apnea syndrome显示文摘 | Carlos Zamarron Luis Valdes Cuadrado Rodolfo Alvarez -Sala | 2013 | Pulm Med2013，2013，52： | 1 |
| 20 | Acute Hemodynamic Response to β-Blockers and Prediction of Long-term Outcome in Primary Prophylaxis of Variceal Bleeding显示文摘 | Càndid Villanueva Carles Aracil Alan Colomo Virginia Hernández–Gea Josep M. López–Balaguer Cristina Alvarez–Urturi Xavier Torras Joaquim Balanzó Carlos Guarner | 2009 | Gastroenterology2009，，1： | 1 |
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