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| 1 | Relationship between intestinal microbiota and ulcerative colitis:Mechanisms and clinical application of probiotics and fecal microbiota transplantation显示文摘Ulcerative colitis(UC) is an inflammatory disease that mainly affects the colon and rectum. It is believed that genetic factors, host immune system disorders, intestinal microbiota dysbiosis, and environmental factors contribute to the pathogenesis of UC. however, studies on the role of intestinal microbiota in the pathogenesis of UC have been inconclusive. Studies have shown that probiotics improve intestinal mucosa barrier function and immune system function and promote secretion of anti-inflammatory factors, thereby inhibiting the growth of harmful bacteria in the intestine. Fecal microbiota transplantation(FMT) can reduce bowel permeability and thus the severity of disease by increasing the production of short-chain fatty acids, especially butyrate, which help maintain the integrity of the epithelial barrier. FMT can also restore immune dysbiosis by inhibiting Th1 differentiation, activity of T cells, leukocyte adhesion, and production of inflammatory factors. Probiotics and FMT are being increasingly used to treat UC, but their use is controversial because of uncertain efficacy. Here, we briefly review the role of intestinal microbiota in thepathogenesis and treatment of UC. | Zhao-hua Shen Chang-xin Zhu Yong-sheng Quan Zhen-yu Yang Shuai Wu Wei-wei Luo Bei Tan Xiao-yan Wang | 2018 | World Journal of Gastroenterology2018，24，1： | 111 |
| 2 | The Relationship between Glucose Excursion and Cognitive Function in Aged Type 2 Diabetes Patients显示文摘Objective Evidence suggests that type 2 diabetes (T2DM) is associated with an increased risk of dementia and that glucose variability is an independent risk factor for diabetic complications.This study investigated the relationship between glucose excursion and cognitive function in aged T2DM patients.Methods A total of 248 aged T2DM patients wore a continuous glucose monitoring system (CGMS) for 3 days in order to evaluate glucose excursion,including mean amplitude of glycemic excursions (MAGE) and mean of daily difference (MODD).All subjects were evaluated with a number of accepted cognitive function tests,including themini‐mental status examination (MMSE).The relationship between MAGE and MODD and performance on these cognitive tests was assessed.Results The MAGE and MMSE score were negatively correlated,likewise with the correlation between MODD and MMSE.Liner multivariate regression analysis showed that MAGE and MODD were also negatively related to MMSE independent of age,sex,glycemic control,hypertension,smoking,or coronary heart disease history.Conclusion Glucose excursion is related to cognitive function in aged T2DM patients.Elevated glucose excursion decreased the MMSE score,which reflects general cognitive function.Thus,therapy aimed at controlling glucose excursion may be beneficial for maintaining cognitive function in aged T2DM patients. | ZHONG Yuan ZHANG Xiao Yan MIAO Ya ZHU Jie Hua YAN Hong WANG Bei Yun JIN Jun HU Ting Jun JIA Wei Ping | 2012 | Biomedical and Environmental Sciences2012，25，1： | 34 |
| 3 | Effect of short-term high-dose atorvastatin on systemic inflammatory response and myocardial ischemic injury in patients with unstable angina pectoris undergoing percutaneous coronary intervention显示文摘 | Sun Fei Yin Zhao Shi Quanxing Zhao Bei Wang Shouli | 2014 | Chinese Medical Journal2014，，21： | 27 |
| 4 | Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor显示文摘 | Lijie Wang Wei Xue Lei Yan Xiaosa Li Jia Wei Miaomiao Chen Jing Wu Bei Yang Li Yang Jia Chen | 2017 | Cell Research2017，27，10： | 26 |
| 5 | Notch signaling mediated by TGF-β/Smad pathway in concanavalin A-induced liver fibrosis in rats显示文摘AIM To explore the exact interaction between Notch and transforming growth factor(TGF)-β signaling in liver fibrosis. METHODS We established a rat model of liver fibrosis induced by concanavalin A. Peripheral blood mononuclear cells(PBMCs) were isolated from the modeled rats, and cultured with γ-secretase inhibitor DAPT and TGF-β inhibitor for 24 h. The m RNA levels of Notch and TGF-β signaling were detected by quantitative real-time polymerase chain reaction. Expression of Notch and TGF-β proteins was analyzed by western blotting.RESULTS Compared to control rats, Notch and TGF-β signaling was activated in PBMCs of model rats. Administration of DAPT and TGF-β inhibitor suppressed Notch and TGF-β signal transducer in PBMCs of model rats. DAPT reduced m RNA and protein expression of TGF-β signaling, such as TGF-β1 and Smad3. TGF-β inhibitor also downregulated Notch1, Hes1 and Hes5, and m RNA and protein expression of the Notch signaling pathway.CONCLUSION Notch and TGF-β signaling play a role in liver fibrosis. TGF-β signaling upregulates Notch signaling, which promotes TGF-β signaling. | Yi Wang Ruo-Wu Shen Bing Han Zhen Li Le Xiong Feng-Yu Zhang Bei-Bei Cong Bei Zhang | 2017 | World Journal of Gastroenterology2017，23，13： | 25 |
| 6 | Dental stem cell and dental tissue regeneration显示文摘The teeth are highly differentiated chewing organs formed by the development of tooth germ tissue located in the jaw and consist of the enamel, dentin, cementum, pulp, and periodontal tissue. Moreover, the teeth have a complicated regulatory mechanism, special histologic origin, diverse structure, and important function in mastication, articulation, and aesthetics. These characteristics, to a certain extent, greatly complicate the research in tooth regeneration. Recently, new ideas for tooth and tissue regeneration have begun to appear with rapid developments in the theories and technologies in tissue engineering. Numerous types of stem cells have been isolated from dental tissue, such as dental pulp stem cells (DPSCs), stem cells isolated from human pulp of exfoliated deciduous teeth (SHED), periodontal ligament stem cells (PDLSCs), stem cells from apical papilla (SCAPs), and dental follicle cells (DFCs). All these cells can regenerate the tissue of tooth. This review outlines the cell types and strategies of stem cell therapy applied in tooth regeneration, in order to provide theoretical basis for clinical treatments. | Qiming Zhai Zhiwei Dong Wei Wang Bei Li Yan Jin | 2019 | Frontiers of Medicine2019，13，2： | 23 |
| 7 | Special Section:SARS-CoV-2 Preliminary evidence from a multicenter prospective observational study of the safety and efficacy of chloroquine for the treatment of COVID-19显示文摘Effective therapies are urgently needed for the SARS-CoV-2 pandemic.Chloroquine has been proved to have antiviral effect against coronavirus in vitro.In this study,we aimed to assess the efficacy and safety of chloroquine with different doses in COVID-19.In this multicenter prospective observational study,we enrolled patients older than 18 years old with confirmed SARS-CoV-2 infection excluding critical cases from 12 hospitals in Guangdong and Hubei Provinces.Eligible patients received chloroquinephosphate s00 mg,orally,once(half dose)or twice(full dose)daily.Patients treated with non-chloroquine therapy were included as historical controls.The primary endpoint is the time to undetectable viral RNA.Secondary outcomes include the proportion of patients with undetectable viral RNA by day 10 and 14,hospitalization time,duration of fever,and adverse events.A total of 197 patients completed chloroquine treatment,and 176patients were included as historical controls.The median time to achieve an undetectable viral RNA was shorter in chloroquine than in non-chloroquine(absolute difference in medians-6.0 days;95%CI-6.0 to—4.0).The duration of fever is shorter in chloroquine(geometric mean ratio 0.6;95%CIO.5 to 0.8).No serious adverse events were observed in the chloroquine group.Patients treated with half dose experienced lower rate of adverse events than with full dose.Although randomized trials are needed for further evaluation,this study provides evidence for safety and efficacy of chloroquine in COVID-19 and suggests that chloroquine can be a cost-effective therapy for combating the COVID-19 pandemic. | Mingxing Huang Man Li Fei Xiao Pengfei Pang Jiabi Liang Tiantian Tang Shaoxuan Liu Binghui Chen Jingxian Shu Yingying You Yang Li Meiwen Tang Jianhui Zhou Guanmin Jiang Jingfen Xiang Wenxin Hong Songmei He Zhaoqin Wang Jianhua Feng Changqing Lin Yinong Ye Zhilong Wu Yaocai Li Bei Zhong Ruilin Sun Zhongsi Hong Jing Liu Huili Chen Xiaohua Wang Zhonghe Li Duanqing Pei Lin Tian Jinyu Xia Shanping Jiang Nanshan Zhong Hong Shan | 2020 | National Science Review2020，7，9： | 20 |
| 8 | Serum and ascites levels of macrophage migration inhibitory factor, TNF-α and IL-6 in patients with chronic virus hepatitis B and hepatitis cirrhosis显示文摘Objective: To study the potential role of macrophagemigration inhibitory factor (MIF), tumor necrosisfactor-α (TNF-α) and interleukin-6 (IL-6) in thedevelopment of chronic virus hepatitis B (CH) andhepatitis cirrhosis (HC).Methods: The serum concentrations of MIF, TNF-αand IL-6 in 18 patients with chronic virus hepatitis Band in 14 patients with hepatitis cirrhosis without as-citic fluid, and the serum and ascites cytokine con-centrations in 22 HC patients with ascitic fluid weredetected by enzyme linked immunity sorbed assay.Results: The cytokine concentrations of the patientswere significantly higher than those of the controls.The serum levels of MIF, TNF-α and IL-6 of the 22patients with ascitic fluid were higer than those of 14HC patients without ascites. In the 18 patients withCH, the serum cytokine concentrations were the low-est. The serum cytokine concentrations of the 22 HCpatients with ascites were significantly higher thanthose of the 14 HC patients without ascites (P<0. 01). Their serum cytokine concentrations were sig-nificantly higher than those in the 18 patients withCH (P<0. 01). The concentration of IL-6 in asciteswas the highest among all the groups. The serum le-vels of MIF, TNF-α and IL-6 are correlated with al-anine aminotransferase (ALT) in the patients withCH, but not in those with HC with or without asci-tes.Conclusions: These results indicated that MIF, TNF-α and IL-6 may participate in the pathologicalprocess of CH and cirrhosis, that IL-6 seems to playan important role in ascites formation, and that se-rum levels of MIF, TNF-α and IL-6 appear to reflectthe severity of tissue injury in HBV disease. | Wei Zhang Bei Yue Gui-Qiang Wang Shu-Lan Lu the Department of Infectious Dispeases, Ruijing Hospital, Shanghai Second Medical University, Shanghai 200025, China Department of Intectious Diseases, Second Affiliated Hospital, Harbin Medical University, Harbin 150086, China | 2002 | Hepatobiliary & Pancreatic Diseases International2002，1，4： | 18 |
| 9 | Ginkgo biloba extract suppresses hypertrophy and extracellular matrix accumulation in rat mesangial cells显示文摘瞄准:在 mesangial 房间在 mesangial 房间的肥大和细胞外的矩阵(ECM ) 的累积上观察白果树 biloba 摘录(EGb ) 的效果。方法:有教养的 mesangial 房间被分派进 7 个组:正常的组,溶剂控制组,高葡萄糖组, EGb 的低剂量组织, EGb 组,的中等剂量高 EGb 组开,并且 captopril 组织。房间 antioxidases, S 阶段百分比和 G (0 ) 的活动 /G (1 ) 阶段百分比,骨胶原 IV 和 laminin, Smad2/3 和 Smad7, TGF 贝它(1 ) mRNA 被不同方法测量。结果:为对待 EGb 的组,当与高度相比葡萄糖组织时, S 阶段的房间百分比被提起, G (0 )/G (1 ) 的百分比被降低。氧化应力的紧张被削弱。Smad2/3 的表示极大地被减少, Smad7 被增加。骨胶原 IV, laminin 和 TGF- 贝它(1 ) mRNA 也被减少。结论:EGb 能在老鼠 mesangial 房间压制房间肥大和 ECM 的累积,它意味着它能在糖尿病的肾病在肾小球硬化症的延期起一个重要作用。 | Jian-yun WANG Xiao-xing YIN Yun-ming WU Dao-quan TANG Yuan-yuan GAO Mei-rong WAN Xiao-yu HOU Bei ZHANG | 2006 | Acta Pharmacologica Sinica2006，27，9： | 18 |
| 10 | Detection of YMDD.mutants using universal template real-time PCR显示文摘瞄准:与长期的肝炎 B 感染在病人在 lamivudine 治疗期间在肝炎 B 和 lamivudine 抵抗的监视器为 lamivudine 抵抗的变化的察觉建立一个快速、精确的方法。方法:我们用一个通用模板和 TaqMan 探查建立了一个即时 PCR 方法检测 YMDD 异种。YVDD 和 YIDD 的变体被单个反应测试(反应 V 和反应我) 并且全部的肝炎 B 为控制(反应 C ) 在另一反应被检测。结果被 deltaCt <
决定 3.5 (反应 V 的 deltaCt = Ct 或我 - 反应 C 的 Ct ) 。包含不同 YMDD 变化的 HBV 聚合酶基因的克隆被测试。从有长期的肝炎 B 感染的 163 个对待 lamivudine 的病人的浆液样品用这个方法被检测,结果被定序的 DNA 证实。结果:同样多每宽类型的原生质标志的毫升象 1000 个拷贝一样被检测,非特定的 priming 被排除。在从与 lamivudine 对待的病人的 163 件样品, lamivudine 抵抗的变化在 51 件样品被检测。结论:这通用即时 PCR 是为在对待 lamivudine 的病人的 HBV 病毒的 YMDD 异种的数量化离子的一个快速、精确的方法并且能被用来监视 lamivudine 抵抗的变化在前并且在 lamivudine 治疗期间。 | Rong-Sheng Wang Hua Zhang Yu-Fen Zhu Bei Han Zhi-Jun Yang | 2006 | World Journal of Gastroenterology2006，12，8： | 17 |
| 11 | Elevated nocturnal and morning blood pressure in patients with obstructive sleep apnea syndrome显示文摘 | HE Quan-ying FENG Jing ZHANG Xilong LIANG Zong-an HUANG Shao-guang KANG Jian WANG Guang-fa ZHANG Li-qiang MA Li-jun WANG Bei LIN Qi-chang ZHANG Jin-nong LIU Hui-guo LUO Yuan-ming LIU Jian-hong WANG Shi XIAO Gao-hui LU Gan ZHANG Jin FENG Xue-wei CHEN Bao-yuan | 2012 | Chinese Medical Journal2012，，10： | 15 |
| 12 | Respective and interactive effects of doubled CO2 and O3 concentration on membrane lipid peroxidation and antioxidative ability of soybean显示文摘Effects of doubled CO2 and O3 concentration on Soybean were studied in open-top chambers (OTC). Under doubled CO2 concentration, grain yield and biomass increased, the SOD activity, vitamin C (Vc) and carotenoid (Car) content also increased; Superoxide (O2-.) generating rate decreased, relative conductivity and malondialdehyde (MDA) content significantly declined.But under doubled O3 concentration, the SOD activity, Vc and Car contents declined, resulting in imbalance of activated-oxygen production, enhanced O2-. generating rate and accelerated process of lipid peroxidation and increase in MDA content and ion leakage of leaves. The final result was decreased grain yield and plant biomass. Interactive effects of doubled CO2 and O3 concentrations on soybean were mostly counteractive. However, the beneficial effects of concentration-doubled CO2 are more than compensate the negative effects imposed by doubled O3, and the latter in its turn partly counteracted the positive effects of the former. | ZHAO Tianhong, SHI Yi, HUANG Guohong, WANG Yan & SUN Bei Key Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China Shenyang Agricultural University, Shenyang 110161, China | 2005 | Science China(Life Sciences)2005，48，z1： | 14 |
| 13 | Differential regulations of blood pressure and perturbed metabolism by total ginsenosides and conventional antihypertensive agents in spontaneously hypertensive rats显示文摘 | Ji-ye AA Guang-ji WANG Hai-ping HAO Qing HUANG Yi-hong LU Bei YAN Wei-bin ZHA Lin-sheng LIU An KANG | 2010 | Acta Pharmacologica Sinica2010，31，8： | 14 |
| 14 | Autophagy-related proteins Beclin-1 and LC3 predict cetuximab efficacy in advanced colorectal cancer显示文摘AIM:To investigate the utility of Beclin-1 and LC3,two autophagy-related proteins,in predicting the cetuximab efficacy in advanced colorectal cancer(ACRC) . METHODS:The data of 85 patients with ACRC treated at the Sun Yat-sen University Cancer Center from March 1,2005 to December 31,2008 were studied,including 45 cases treated with cetuximab-containing chemotherapy and 40 cases treated with non-cetuximab-containing chemotherapy.Beclin-1 and LC3 expression was evaluated by immunohistochemistry,and KRAS status was evaluated by polymerase chain reaction. RESULTS:Beclin-1 and LC3 expression in ACRC wassignificantly correlated(r=0.44,P<0.01);however,LC3 was more highly expressed in cancerous tissues than in normal tissues(Z=-2.63,P<0.01) .In the cetuximab-containing chemotherapy group,patients with low LC3 expression had higher objective response rates(ORRs) than those with high LC3 expression(52.9%vs 17.9%,P=0.01) ,and patients with low Beclin-1 expression had a longer median progressionfree survival(PFS) than their counterparts with higher Beclin-1 expression(9.0 mo vs 3.0 mo,P=0.01) . However,neither of these predictive relationships was detected in the group treated with non-cetuximabcontaining chemotherapy.Patients with wild-type KRAS had higher ORRs(42.3%vs 9.1%,P=0.049) and disease control rates(DCRs)(73.1%vs 36.4%,P= 0.035) ,and longer median PFS(5.5 mo vs 2.5 mo,P= 0.02) than those with mutant KRAS in the cetuximabcontaining chemotherapy group.Neither Beclin-1(P= 0.52) nor LC3(P=0.32) expression was significantly correlated with KRAS status. CONCLUSION:Patients with low Beclin-1 expression had a longer PFS than those with high Beclin-1 expression,and patients with low LC3 expression had a higher ORR in ACRC patients treated with cetuximab-containing chemotherapy. | Gui-Fang Guo Wen-Qi Jiang Bei Zhang Yu-Chen Cai Rui-Hua Xu Xu-Xian Chen Fang Wang Liang-Ping Xia | 2011 | World Journal of Gastroenterology2011，17，43： | 14 |
| 15 | Drug-resistant genes carried by Acinetobacter baumanii isolated from patients with lower respiratory tract infection显示文摘 | DAI Ning LI De-zhi CHEN Ji-chao CHEN Yu-sheng GENG Rong HU Ying-hui YANG Jing-ping DU Juan HU Cheng-ping ZHANG Wei LI Jia-shu YU Qin WAN Huan-ying MU Lan ZHONG Xiao-ning WEI Li-ping MA Jian-jun WANG Qiu-yue HU Ke TIAN Gui-zhen CAI Shao-xi WANG Rui-qin HE Bei WANG Si-qin WANG Zhan-wei ZHAO Su-rui GAO Zhan-cheng | 2010 | Chinese Medical Journal2010，，18： | 13 |
| 16 | Factors Associated with Thrombolysis Outcome in Ischemic Stroke Patients with Atrial Fibrillation显示文摘The outcome of early intravenous thrombolysis for ischemic stroke in patients with atrial fibrillation(AF)is worse than that without thrombosis. How to increase the efficacy of intravenous thrombolysis for AF-related ischemic stroke remains largely unknown. In this study, we investigated factors that influence the effect of intravenous thrombolysis in these patients. Our results showed that thrombolysis was independently associated with a favorable outcome(P \ 0.001) and did not influence the mortality of AF-related ischemic stroke, although it increased the risk of hemorrhage within 24 h after treatment. Risk factors for a poor outcome at admission were:heart failure(P = 0.045); high systolic pressure(P = 0.039); high blood glucose(P = 0.030); and a high National Institutes of Health Stroke Scale(NIHSS) score(P \ 0.001). Moreover, high systolic pressure at admission(P = 0.007), high blood glucose(P = 0.027), and a high NIHSS score(P \ 0.001) were independent risk factors for mortality at 3 months. Besides thrombolysis, a high NIHSS score(P = 0.006) and warfarin taken within 48 h before stroke onset(P = 0.032) were also independent risk factors for symptomatic hemorrhage within 24 h after treatment. Ischemic stroke patients with AF benefited from intravenous thrombolysis with recombinant tissue plasminogen activator within 4.5 h after stroke. | Qiuyun Zhao Xiaobo Li Wanli Dong Min Ye Yongjun Cao Meijuan Zhang Qiantao Cheng Junshan Zhou Guofang Chen Ming Yu Shanshan Hong Xiue Wei Bei Wang Guiyun Cui Peng Zhang Hong Ding Rongzhen Xu Yan Chen Yun Xu | 2016 | Neuroscience Bulletin2016，32，2： | 13 |
| 17 | Dynamic changes of IL-2/IL-10, sFas and expression of Fas in intestinal mucosa in rats with acute necrotizing pancreatitis显示文摘AIM:To investigate dynamic changes of serum IL-2, IL-10, IL-2/IL-10 and sFas in rats with acute necrotizing pancreatitis. To explore the expression of Fas in intestinal mucosa of rats with acute necrotizing pancreatitis (ANP). METHODS:A total of 64 Sprague-Dawley (SD) rats were randomly divided into two groups:normal control group (C group), ANP group (P group). An ANP model was induced by injection of 50 g/L sodium taurocholate under the pancreatic membrane. Normal control group received isovolumetric injection of 9 g/L physiological saline solution using the same method. The blood samples of the rats in each group were obtained via superior mesenteric vein to measure levels of IL-2, IL-10, sFas and calculate the value of IL-2/IL-10. The levels of IL-2, IL-10 and sFas were determined by ELISA. The severity of intestinal mucosal injury was evaluated by pathologic score. The expression of Fas in intestinal mucosal tissue was determined by immunohistochemistry staining. RESULTS:Levels of serum IL-2 were significantly higher in P group than those of C group (2.79 ± 0.51 vs 3.53 ± 0.62, 2.93 ± 0.89 vs 4.35 ± 1.11, 4.81 ± 1.23 vs 6.94 ± 1.55 and 3.41 ± 0.72 vs 4.80 ± 1.10, respectively, P < 0.01, for all) and its reached peak at 6 h. Levels of serum IL-10 were significantly higher in P group than those of C group at 6 h and 12 h (54.61 ± 15.81 vs 47.34 ± 14.62, 141.15 ± 40.21 vs 156.12 ± 43.10, 89.18 ± 32.52 vs 494.98 ± 11.23 and 77.15 ± 22.60 vs 93.28 ± 25.81, respectively, P < 0.01, for all). The values of IL-2/IL-10 were higher significantly in P group than those of C group at 0.5 h and 2 h (0.05 ± 0.01 vs 0.07 ± 0.02 and 0.02 ± 0.01 vs 0.03 ± 0.01, respectively, P < 0.01, for all), and it were significantly lower than those of C group at 6 h (0.05 ± 0.02 vs 0.01 ± 0.01, P < 0.01) and returned to the control level at 12 h (0.04 ± 0.01 vs 0.05 ± 0.02, P > 0.05). In sFas assay, there was no significant difference between P group and C group (3.16 ± 0.75 vs 3.31 ± 0.80, 4.05 ± 1.08 vs 4.32 ± 1.11, 5.93 ± 1.52 vs 5.41 ± 1.47 and 4.62 ± 1.23 vs 4.44 ± 1.16, respectively, P > 0.05, for all). Comparison of P group and C group, the pathological changes were aggravated significantly in P group. Immunohistochemistry staining show the expression of Fas was absent in normal intestinal tissues, however, it gradually increased after induction of pancreatitis in intestinal tissue, then reached their peaks at 12 h.CONCLUSION:Fas were involved in the pathogenesis of pancreatitis associated intestinal injury. The mechanisms of Fas may be associated to Fas mediated T helper cell apoptosis. | Sheng-Chun Dang Jian-Xin Zhang Jian-Guo Qu Zheng-Fa Mao Xu-Qing Wang Bei Zhu | 2008 | World Journal of Gastroenterology2008，14，14： | 12 |
| 18 | Microstructure and mechanical properties of FeCoNiCr high-entropy alloy strengthened by nano-Y_2O_3 dispersion显示文摘In this manuscript, the FeCrCoNi high entropy alloy strengthened by nano-Y_2 O_3 particles was synthesized via mechanical alloying and spark plasma sintering. Effects of Y_2 O_3 additions on the microstructure and mechanical properties were investigated.The oxide-dispersion-strengthened(ODS) high-entropy alloy bearing 5 wt% Y_2 O_3 was found to have a finer grain with respect to the single-phase face-centered cubic(FCC) alloy without oxides. Consequently, the yield strength increased from 654 to1754 MPa, and the fracture strength reached up to 2325 MPa while the plasticity decreased appreciably for the ODS high-entropy alloy. The synergic effects of grain size hardening and particle strengthening on mechanical properties of the ODS HEA were discussed. This finding indicates that the addition of nano-scaled oxides is an effective and feasible approach to designing HEAs with superior mechanical properties. | jia bei liu xiongjun wang hui wu yuan lu zhaoping | 2018 | Science China(Technological Sciences)2018，61，2： | 12 |
| 19 | DL-3-n-butylphthalide protects the blood-brain barrier against ischemia/hypoxia injury via upregulation of tight junction proteins显示文摘Background:The increased permeability of the blood-brain barrier (BBB) induced by ischemia/hypoxia is generally correlated with alteration of tight junctions (TJs). DL-3-n-butylphthalide (NBP) has been shown to exert neuroprotective effects after ischemic injury. However, few studies have assessed the correlation between NBP and TJs. This study aimed to investigate the potential effect of NBP on the TJ proteins claudin-5, zonula occludens-1 (ZO-1), and occludin during brain ischemia. Methods: A chronic cerebral hypoperfusion (CCH) Sprague-Dawley rat model was established, and NBP (20, 40, or 80 mg/kg, gavage, once a day) treatment was performed for 14 days. NBP (0.1 or 1.0μmol/L) pre-treatment was applied to an in vitro hypoxia microvascular endothelial cell model (1%〇2, 24 h). BBB permeability was assessed by performing the Evans blue assay. The expressions and localization of claudin-5, ZO-1, occludin, phosphorylated/total protein kinase B (p-Akt/Akt), phosphorylated/total glycogen synthase kinase 3p (GSK-3(3)/GSK-3p, and (3-catenin/p-actin were evaluated by Western blotting or immunofluorescence. Reactive oxygen species (ROS) generation was measured by flow cytometry analysis. TJ ultrastructure was observed by transmission electron microscopy. Results: In CCH rats, treatment with 40 and 80 mg/kg NBP decreased the Evans blue content in brain tissue (9.0 ± 0.9 (μg/g vs. 12.3 ± 1.9 (μg/g, P = 0.005;6.7 ± 0.6 μg/g vs. 12.3 ± 1.9μg/g, P < 0.01), increased the expression of claudin-5 (0.79 ± 0.08 mvs. 0.41 ± 0.06, P < 0.01;0.07 ± 0 .0 7 vs. 0.41 ± 0 .0 6 , P < 0 .61 ), and elevated the ZO-1 protein level (P < 0.05) in brain microvascular segments in a dose-dependent manner in comparison with the corresponding values in the model group. There was no significant difference in occludin expression (P > 0.05). In the hypoxia cell model, NBP pre-treatment improved TJ ultrastructure, decreased intracellular ROS level, and increased the expression of claudin-5 (P < 0.01) and ZO-1 (P < 0.01) in comparison with the corresponding values in the hypoxia group. NBP treatment also elevated the relative expression levels of p-Akt/Akt, p-GSK-3p/GSK-3β, and β-catenin/β-actin in comparison with the corresponding values in the hypoxia group (all P < 0 .0 5 ). Conclusion: NBP improves the barrier function of BBB against ischemic injury by upregulating the expression of TJ proteins, possibly by reducing oxidative stress and activating the Akt/GSK-3β/β-catenin signaling pathway. | Zhan-Ying Ye Han-Ying Xing Bei Wang Min Liu Pei-Yuan Lv | 2019 | Chinese Medical Journal2019，，11： | 12 |
| 20 | CD59 Silencing via Retrovirus-Mediated RNA Interference Enhanced Complement-Mediated Cell Damage in Ovary Cancer显示文摘CD59,belonging to membrane complement regulatory proteins(mCRPs) ,inhibits the cytolytic activity of complement and is over-expressed in solid cancers,including ovary cancer. The aim of the present study was to construct recombinant retrovirus encoding shRNA targeted human CD59 and infect A2780 cells in order to investigate the relationship between decreased CD59 expression and tumorigenesis of ovary cancer. siCD59 and siCD59-C were successfully constructed and identified by PCR,restriction endonuclease analyses and DNA sequencing,respectively. The siCD59 was able to efficiently infect A2780 cells,which was confirmed by Western blotting. When incubated with fresh normal human serum(8%,v/v) for 1 h at 37°C,the cell viability was decreased and cell damage was increased in siCD59 infected A2780 cells compared to siCD59-C infected cells. This led to the activation of caspase-3. The apoptosis in siCD59 infected cells was shown with hypercondensed nuclei using Hoechst staining. Meanwhile,the weight of ovary tumor graft in nude mice was significantly decreased in siCD59 group compared to that of siCD59-C group. And the expression of CD59 protein in tumor tissue in siCD59 group was significantly decreased. These results suggested that CD59 silencing in ovary cancer cells via retrovirusmediated RNAi can enhance complement-mediated cell damage,inhibiting growth of ovary cancer. CD59 might be a potential target for gene therapy in ovary cancer. | Xuexiang Shi Bei Zhang Jinlin Zang Guoying Wang Meihua Gao | 2009 | Cellular & Molecular Immunology2009，6，1： | 12 |
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