| 2 | The SARS-CoV-2 spike L452R-E484Q variant in the Indian B.1.617 strain showed significant reduction in the neutralization activity of immune sera显示文摘To assess the impact of the key non-synonymous amino acid substitutions in the RBD of the spike protein of SARS-CoV-2 variant B.1.617.1(dominant variant identified in the current India outbreak)on the infectivity and neutralization activities of the immune sera,L452R and E484Q(L452R-E484Q variant),pseudotyped virus was constructed(with the D614G background).The impact on binding with the neutralizing antibodies was also assessed with an ELISA assay.Pseudotyped virus carrying a L452R-E484Q variant showed a comparable infectivity compared with D614G.However,there was a significant reduction in the neutralization activity of the immune sera from non-human primates vaccinated with a recombinant receptor binding domain(RBD)protein,convalescent patients,and healthy vaccinees vaccinated with anmRNA vaccine.In addition,there was a reduction in binding of L452R-E484Q-D614G protein to the antibodies of theimmune sera fromvaccinated nonhuman primates.These results highlight the interplay between infectivity and other biologic factors involved in the natural evolution of SARS-CoV-2.Reduced neutralization activities against the L452R-E484Q variant will have an impact on health authority planning and implications for the vaccination strategy/newvaccine development. | Gen Li Zhongcheng Zhou Peng Du Meixing Yu Ning Li Xinxin Xiong Hong Huang Zhihai Liu Qinjin Dai Jie Zhu Chengbin Guo ShanyunWu Daniel T.Baptista-Hon Man Miao LamWai Ming Yong Wu Fanxin Zeng Charlotte L.Zhang Edward D.Zhang Haifeng Song Jianghai Liu Johnson Yiu-Nam Lau Andy P.Xiang Kang Zhang | 2021 | Precision Clinical Medicine2021，4，3： | 0 |
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