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| 1 | Making a tooth:growth factors,transcription factors,and stem cells显示文摘Mammalian tooth development is largely dependent on sequential and reciprocal epithelial-mesenchymal interactions. These processes involve a series of inductive and permissive interactions that result in the determination, differentiation, and organization of odontogenic tissues. Multiple signaling molecules, including BMPs, FGFs, Shh, and Wnt proteins, have been implicated in mediating these tissue interactions. Transcription factors participate in epithelial-mesenchymal interactions via linking the signaling loops between tissue layers by responding to inductive signals and regulating the expression of other signaling molecules. Adult stem cells are highly plastic and multipotent. These cells including dental pulp stem cells and bone marrow stromal cells could be reprogrammed into odontogenic fate and participated in tooth formation. Recent progress in the studies of molecular basis of tooth development, adult stem cell biology, and regene- ration will provide fundamental knowledge for the realization of human tooth regeneration in the near future. | YanDingZHANG ZhiCHEN YiQiangSONG ChaoLIU YiPingCHEN | 2005 | Cell Research2005，15，5： | 36 |
| 2 | Endothelium-specific SIRT1 overexpression inhibits hyperglycemia-induced upregulation of vascular cell senescence显示文摘The rapidly increasing prevalence of diabetes mellitus worldwide is one of the most serious and challenging health problems in the 21st century.Mammalian sirtuin 1(SIRT1) has been shown to decrease high-glucose-induced endothelial cell senescence in vitro and prevent hyperglycemia-induced vascular dysfunction.However,a role for SIRT1 in prevention of hyperglycemia-induced vascular cell senescence in vivo remains unclear.We used endothelium-specific SIRT1 transgenic(SIRT1-Tg) mice and wild-type(WT) mice to construct a 40-week streptozotocin(STZ)-induced diabetic mouse model.In this mode,42.9% of wild-type(WT) mice and 38.5% of SIRT1-Tg mice were successfully established as diabetic.Forty weeks of hyperglycemia induced significant vascular cell senescence in aortas of mice,as indicated by upregulation of expression of senescence-associated markers including p53,p21 and plasminogen activator inhibitor-1(PAI-1).However,SIRT1-Tg diabetic mice displayed dramatically decreased expression of p53,p21 and PAI-1 compared with diabetic WT mice.Moreover,manganese superoxide dismutase expression(MnSOD) was significantly downregulated in the aortas of diabetic WT mice,but was preserved in diabetic SIRT1-Tg mice.Furthermore,expression of the oxidative stress adaptor p66Shc was significantly decreased in aortas of SIRT1-Tg diabetic mice compared with WT diabetic mice.Overall,these findings suggest that SIRT1-mediated inhibition of hyperglycemia-induced vascular cell senescence is mediated at least partly through the reduction of oxidative stress. | CHEN HouZao WAN YanZhen ZHOU Shuang LU YunBiao ZHANG ZhuQin ZHANG Ran CHEN Feng HAO DeLong ZHAO Xiang GUO ZhiChen LIU DePei LIANG ChihChuan | 2012 | Science China(Life Sciences)2012，55，6： | 14 |
| 3 | Inhibition of human La protein by RNA interference downregulates hepatitis B virus mRNA in 2.2.15 cells显示文摘AIM: To investigate the role of human La protein in HBV mRNA expression.METHODS: Three human La protein (hLa) specific siRNA expression cassettes (SECs) containing U6+1 promoter were prepared via one-step overlapping extension PCR. After transfection with SECs into HepG2 cells, inhibition effects on hLa expression were analyzed by semi-quantitative RT-PCR and Western blotting. Then, effective SECs were screened out and transfected into 2.2.15 cells, a stable HBV-producing cell line. HBV surface antigen(HBsAg) and e antigen (HBeAg) secretions into culture media were detected by microparticle enzyme immunoassay (MEIA) and HBs and HBe mRNA levels were analyzed by semi-quantitative RT-PCR.RESULTS: SEC products containing U6+1 snRNA promoter,and 3 sites of hLa mRNA specific siRNA were obtained successfully by one-step overlapping extension PCR and could be directly transfected into HepG2 cells, resulting in inhibition of La protein expression in both mRNA and protein levels, among which U6+l-hLa833 was the most efficient,which reduced 18.6-fold mRNA and 89% protein level respectively. In 2.2.15 cells, U6+l-hLa833 was also efficient on inhibition of hLa expression. Furthermore, semi-quantitative RT-PCR showed that HI3s and HBe mRNA levels were significantly decreased by 8-and 66-fold in U6+l-hLa833 transfected cells compared to control. Accordingly, HBsAg and HBeAg secretions were decreased partly posttransfection with SECs.CONCLUSION: PCR-based SECs can be used to mediate RNAi in mammalian cells and provide a novel approach to study the function of La protein. The inhibition of La protein expression can result in a significant decrease ofHBV mRNA, which implies that the hLa protein is also involved HBV RNA metabolism as one of the HBV RNA-stabilizing factors in human cells. | QinNi ZhiChen Hang-PingYao Zheng-GangYang Ke-ZhouLiu Ling-LingWu | 2004 | World Journal of Gastroenterology2004，10，14： | 13 |
| 4 | Late Eocene pollen records and palaeoenvironmental changes in northern Tibetan Plateau显示文摘The Eocene palaeovegetation landscape and palaeoclimate reconstructed from the pollen records in the Jiuquan Basin, northwest China provide some important information on the early uplift of the Tibetan Plateau and the origin and evolution of the aridification in northwest China. The records show the arid-semiarid scrubs with open forest palynofloras controlled by the subtropical high existed in northwest China during the 40.2-33.4 Ma. Four pollen assemblages are found: Nitrariadites-Cheno-podipollis-Pinaceae assemblage (40.2-37.9 Ma) is followed by Chenopodipollis-Nitrariadites assemblage (37.9-34.6 Ma), Pinuspollenites & Abietineaepollenites-Chenopodipollis assemblage (34.6-33.9 Ma), and Chenopodipollis-Nitrariadites assemblage (33.9-33.4 Ma). The percentage of thermophilic types is in anti-correlation with that of the dry types, which means the palaeoclimate is relatively warmwet or cold-dry during most of that time. Such aridity may be related to the water vapor reduction and the planetary wind system movement northward in response to the cooling caused by small-ephemeral icesheets. | MIAO YunFa FANG XiaoMin SONG ZhiChen WU FuLi HAN WenXia DAI Shuang SONG ChunHui | 2008 | Science China Earth Sciences2008，51，8： | 10 |
| 5 | An overview of graphene-based hydroxyapatite composites for orthopedic applications显示文摘Hydroxyapatite(HA)is an attractive bioceramic for hard tissue repair and regeneration due to its physicochemical similarities to natural apatite.However,its low fracture toughness,poor tensile strength and weak wear resistance become major obstacles for potential clinical applications.One promising method to tackle with these problems is exploiting graphene and its derivatives(graphene oxide and reduced graphene oxide)as nanoscale reinforcement fillers to fabricate graphene-based hydroxyapatite composites in the form of powders,coatings and scaffolds.The last few years witnessed increasing numbers of studies on the preparation,mechanical and biological evaluations of these novel materials.Herein,various preparation techniques,mechanical behaviors and toughen mechanism,the in vitro/in vivo biocompatible analysis,antibacterial properties of the graphene-based HA composites are presented in this review. | Ming Li Pan Xiong Feng Yan Sijie Li Changhong Ren Zhichen Yin Ang Li Huafang Li Xunming Ji Yufeng Zheng Yan Cheng | 2018 | Bioactive Materials2018，3，1： | 9 |
| 6 | Diffraction-limited imaging with monolayer 2D material-based ultrathin flat lenses显示文摘Ultrathin flat optics allow control of light at the subwavelength scale that is unmatched by traditional refractive optics.To approach the atomically thin limit,the use of 2D materials is an attractive possibility due to their high refractive indices.However,achievement of diffraction-limited focusing and imaging is challenged by their thickness-limited spatial resolution and focusing efficiency.Here we report a universal method to transform 2D monolayers into ultrathin flat lenses.Femtosecond laser direct writing was applied to generate local scattering media inside a monolayer,which overcomes the longstanding challenge of obtaining sufficient phase or amplitude modulation in atomically thin 2D materials.We achieved highly efficient 3D focusing with subwavelength resolution and diffractionlimited imaging.The high focusing performance even allows diffraction-limited imaging at different focal positions with varying magnifications.Our work paves the way for downscaling of optical devices using 2D materials and reports an unprecedented approach for fabricating ultrathin imaging devices. | Han Lin Zai-Quan Xu Guiyuan Cao Yupeng Zhang Jiadong Zhou Ziyu Wang Zhichen Wan Zheng Liu Kian Ping Loh Cheng-Wei Qiu Qiaoliang Bao Baohua Jia | 2020 | Light(Science & Applications)2020，9，1： | 9 |
| 7 | Inhibition of hepatitis B virus surface antigen expression by small hairpin RNA in vitro显示文摘AIM: To explore the anti-hepatitis B virus effect of RNA interference (RNAi) using small hairpin RNA (shRNA)expression vector.METHODS: Hepatitis B virus surface antigen green fluorescent protein (HBs-GFP) fusion vector and shRNA expression vectors were constructed and cotransfected transiently into HepG2 cells. mRNAs extracted from HepG2 cells were detected by real-time PCR. Fluorescence of HBs-GFP protein was detected by fluorescence-activated cell sorting (FACS). The effective shRNA expression vector was transfected into HepG2.2.15 cells. HBsAg and HBeAg in HepG2.2.15 cells were analyzed by radioimmunoassay (RIA) method.RESULTS: FACS revealed that shRNA targeting at HBsAg reduced the GFP signal by 56% compared to the control.Real-time PCR showed that HBs-GFP mRNA extracted from HepG2 cells cotransfected with pAVU6+27 and HBs-GFP expression plasmids decreased by 90% compared to the empty vector control. The expressions of HBsAg and HBeAg were also inhibited by 43% and 64%, respectively.CONCLUSION: RNAi using shRNA expression vector can inhibit the expression of HBsAg, providing a fresh approach to screening the efficient small interfering RNAs (siRNAs). | Zheng-GangYang ZhiChen QinNi NingXu Jun-BinShao Hang-PingYao | 2005 | World Journal of Gastroenterology2005，11，4： | 8 |
| 8 | Overactive bladder symptom score to evaluate efficacy of solifenacin for the treatment of overactive bladder symptoms显示文摘 | Liu Ming Wang Jianye Yang Yong An Ruihua Wen Jianguo Guan Zhichen Zheng Shaobin Wang Dongwen Song Bo Liao Limin Guo Hongqian Xiao Jiaquan Sun Yinghao Shen Zhoujun Kong Chuize He Dalin Huang Yiran Wang Xiaofeng Zhang Xu Li Hanzhong Huang Jian Zhao Xiaokun Zeng Puqing Song Xishuang Ye Zhangqun | 2014 | Chinese Medical Journal2014，，2： | 7 |
| 9 | Association between TRAIL expression on peripheral blood lymphocytes and liver damage in chronic hepatitis B显示文摘AIM: To explore a novel mechanism for tumor necrosis factorrelated apoptosis-inducing ligand (TRAIL), upregulation of CD4+ and CD8+T lymphocytes participating in the pathophysiological process of chronic hepatitis B (CHB).METHODS: The levels of serum soluble TRAIL (sTRAIL),serum IFN-γ and membrane-bound TRAIL expression on peripheral leucocytes from 58 CHB patients were examined ELISA and flow cytometry respectively. The levels of TRAIL were compared with the baseline levels of 17 healthy controls, and correlation analysis was performed between ALT, TBIL, PT, morphological change in hepatic tissues, and serum IFN-γ.RESULTS: The results showed that TRAIL levels on membranes of CD4+, CD8+ T cells in CHB patients were much higher than those in healthy controls (P<0.001),and were correlated with serum TBIL (r = 0.354, P = 0.008for CD4+ and r = 0.522, P = 0.000 for CD8+, respectively),ALT (r = 0.393, P = 0.003 for CD8+), PT (r = 0.385,P = 0.004 for CD8+) and serum IFN-γ level (r = 0.302,P = 0.011 for CD4+ and r= 0.307, P = 0.009 for CD8+).On the contrary to membrane-bound TRAIL expression,serum level of sTRAIL was not correlated with that of TBIL and PT, though it was higher than that of the normal population and was positively correlated with serum HBeAg expression (r = 0.695, P = 0.001).CONCLUSION: The expression level of TRAIL on the membrane of lymphocytes was upregulated and associated with the liver injury in CHB patients. These findings suggest that upregulation of TRAIL expression may be induced by virus antigen and inflammatory cytokine IFN-γ. | Gong-YingChen Jian-QinHe Guo-CaiLu Ming-WeiLi Chen-HuaiXu Wei-WeiFan ChenZhou ZhiChen | 2005 | World Journal of Gastroenterology2005，11，26： | 6 |
| 10 | Targeting tumor cells with antibodies enhances anti-tumor immunity显示文摘Tumor-targeting antibodies were initially defined as a group of therapeutic monoclonal antibodies (mAb)that recognize tumor-specific membrane proteins,block cell signaling,and induce tumor-killing through Fc-driven innate immune responses.However,in the past decade,ample evidence has shown that tumor-targeting mAb (TTmAb)eradicates tumor cells via activation of cytotoxic T cells (CTLs).In this review,we specifically focus on how TTmAbs induce adaptive anti-tumor immunity and its potential in combination therapy with immune cytokines,checkpoint blockade,radiation,and enzymetargeted small molecule drugs.Exploring the mechanisms of these preclinical studies and retrospective clinical data will significantly benefit the development of highly efficient and specific TTmAb-oriented anti-tumor remedies. | Zhichen Sun Yang-Xin FU Hua Peng | 2018 | Biophysics Reports2018，4，5： | 4 |
| 11 | Aboveground biomass and its spatial distribution pattern of herbaceous marsh vegetation in China显示文摘Herbaceous marsh is the most widely distributed type of marsh wetland ecosystem,and has important ecological functions such as water conservation,climate regulation,carbon storage and fixation,and sheltering rare species.The carbon sequestration function of herbaceous marsh plays a key role in slowing climate warming and maintaining regional environmental stability.Vegetation biomass is an important index reflecting the carbon sequestration capacity of wetlands.Investigating the biomass of marsh vegetation can provide a scientific basis for estimating the carbon storage and carbon sequestration capacity of marshes.Based on field survey data of aboveground biomass of herbaceous marsh vegetation and the distribution data set of marsh in China,we analyzed the aboveground biomass and its spatial distribution pattern of herbaceous marsh on a national scale for the first time.The results showed that in China the total area of herbaceous marsh was 9.7×10^(4) km^(2),the average density of aboveground biomass of herbaceous marsh vegetation was 227.5±23.0 g C m-2(95%confidence interval,the same below),and the total aboveground biomass was 22.2±2.2 Tg C(1 Tg=1012 g).The aboveground biomass density of herbaceous marsh vegetation is generally low in Northeast China and the Tibetan Plateau,and high in central North China and coastal regions in China.In different marsh distribution regions of China,the average biomass density of herbaceous marsh vegetation from small to large was as follows:temperate humid and semi-humid marsh region(182.3±49.3 g C m^(-2)) | Xiangjin SHEN Ming JIANG Xianguo LU Xingtu LIU Bo LIU Jiaqi ZHANG Xianwei WANG Shouzheng TONG Guangchun LEI Shengzhong WANG Chuan TONG Hangqing FAN Kun TIAN Xiaolong WANG Yuanman HU Yonghong XIE Muyuan MA Shuwen ZHANG Chunxiang CAO Zhichen WANG | 2021 | Science China Earth Sciences2021，64，7： | 4 |
| 12 | The first pulsar discovered by FAST显示文摘To assist with the commissioning [1] of the Five-hundredmeter Aperture Spherical radio Telescope (FAST), we performed a pulsar search, with the primary goal of developing and testing the pulsar data acquisition and processing pipelines. We tested and used three pipelines, two (Pl and P2 hereafter) searched for the periodic signature of pulsars whereas the other one was used to search for bright single pulses (P3 hereafter). | Lei Qian ZhiChen Pan Di Li George Hobbs WeiWei Zhu Pei Wang ZhiJie Liu YouLing Yue Yan Zhu HongFei Liu DongJun Yu JingHai Sun Peng Jiang GaoFeng Pan Hui Li HengQian Gan Rui Yao XiaoYao Xie Fernando Camilo Andrew Cameron Lei Zhang Shen Wang FAST Collaboration | 2019 | Science China(Physics,Mechanics & Astronomy)2019，62，5： | 3 |
| 13 | Machine learning-based real-time visible fatigue crack growth detection显示文摘Many large-scale and complex structural components are applied in the aeronautics and automobile industries.However,the repeated alternating or cyclic loads in service tend to cause unexpected fatigue fractures.Therefore,developing real-time and visible monitoring methods for fatigue crack initiation and propagation is critically important for structural safety.This paper proposes a machine learning-based fatigue crack growth detection method that combines computer vision and machine learning.In our model,computer vision is used for data creation,and the machine learning model is used for crack detection.Then computer vision is used for marking and analyzing the crack growth path and length.We apply seven models for the crack classification and find that the decision tree is the best model in this research.The experimental results prove the effectiveness of our method,and the crack length measurement accuracy achieved is 0.6 mm.Furthermore,the slight machine learning models help us realize real-time and visible fatigue crack detection. | Le Zhang Zhichen Wang Lei Wang Zhe Zhang Xu Chen Lin Meng | 2021 | Digital Communications and Networks2021，7，4： | 2 |
| 14 | Process optimization,texture and microstructure of novel kelp tofu显示文摘Laminaria japonica Aresch as an edible and medicine dual-purpose marine algae,has been gradually accepted by people.Tofu was Chinese traditional food,combining kelp function ingredients with tofu has practical value.The extraction-optimization methodology and the sequences of kelp antihypertensive peptide(KAP)were investigated.The sensory,whiteness,water retention ability(WRA)and microstructure of KAP tofu were evaluated.The scanning electron microscope(SEM)results showed KAP addition(20%)significantly improved the tofu texture,with a denser network composed of relatively even pores.Compared with gypsum tofu and glucose-δ-interior fat(GDL)tofu,KAP tofu had significantly higher WRA(94.49±0.49)%and sensory evaluation score value(92.54±0.52),and a significantly lower IC_(50) value at(2.06±0.04)mg/mL(P<0.05).The maximum IC_(50) value(4.14 mg/mL)of kelp enzymatic hydrolysate was observed at enzyme(at an alkaline protease:trypsin ratio of 2:1)concentration 1.5%,temperature 55℃ and time 2 h.The sequences of 8 identified peptides were Lys-Tyr,Phe-Tyr,Gly-Lys-Tyr,Ala-Lys-Tyr,Ser-Lys-Thr-Tyr,Lys-Lys-Phe-Tyr,Lys-Phe-Lys-Tyr and Ala-Lys-Tyr-Ser-Tyr with IC_(50) values of 5.24,4.83,7.94,7.52,20.63,15.33,10.73 and 2.42μmol/L,respectively.These results indicated the potential use of KAP tofu as a valuable resource for development of functional foods. | Xianjiang Ye Li Chen Zhichen Su Xiaojuan Lin Jicheng Chen | 2023 | Food Science and Human Wellness2023，12，1： | 2 |
| 15 | FAST: Its Scientific Achievements and Prospects显示文摘FAST is the largest single-dish radio telescope in the world.The characteristics of FAST are presented and analyzed in the context of the parameter space to show how FAST science achievements are affected.We summarize the scientific achievements of FAST and discuss its future science based on the new parts of the parameter space that can be explored by FAST. | Lei Qian Rui Yao Jinghai Sun Jinlong Xu Zhichen Pan Peng Jiang | 2020 | The Innovation2020，1，3： | 2 |
| 16 | Application of fluorescence in situ hybridization in the detection of bladder transitional-cell carcinoma: A multi-center clinical study based on Chinese population显示文摘Objective:To evaluate the diagnostic value of fluorescence in situ hybridization(FISH)in bladder cancer.Methods:We enrolled healthy volunteers and patients who were clinically suspected to have bladder cancer and conducted FISH tests and cytology examinations from August 2007 to December 2008.Receiver operating characteristic(ROC)curve analysis was performed and the area under curve(AUC)values were calculated for both the FISH and urine cytology tests.Results:A cohort of 988 healthy volunteers was enrolled to establish a reference range for the normal population.A total of 4807 patients with hematuria were prospectively,randomly enrolled for the simultaneous analysis of urine cytology,FISH testing,and a final diagnosis as determined by the pathologic findings of a biopsy or a surgically-excised specimen.Overall,the sensitivity of FISH in detecting transitional-cell carcinoma was 82.7%,while that of cytology was 33.4%(p<0.001).The sensitivity values of FISH for non-muscle invasive and muscle invasive bladder transitional-cell carcinoma were 81.7%and 89.6%,respectively(p=0.004).The sensitivity values of FISH for low and high grade bladder cancer were 82.6%and 90.1%,respectively(p=0.002).Conclusion:FISH is significantly more sensitive than voided urine cytology for detecting bladder cancer in patients evaluated for gross hematuria at all cancer grades and stages.Higher sensitivity using FISH was obtained in high grade and muscle invasive tumors. | Liqun Zhou Kaiwei Yang Xuesong Li Yi Ding Dawei Mu Hanzhong Li Yong Yan Jinyi Li Dongwen Wang Wei Li Yulong Cong Jiangping Gao Kewei Ma Yajun Xiao Sheng Zhang Hongyi Jiang Weilie Hu Qiang Wei Xunbo Jin Zhichen Guan Qingyong Liu Danfeng Xu Xin Gao Yongguang Jiang Weimin Gan Guang Sun Qing Wang Yanhui Liu Jianquan Hou Liping Xie Xishuang Song Fengshuo Jin Jiafu Feng Ming Cai Zhaozhao Liang Jie Zhang Dingwei Ye Lin Qi Lulin Ma Jianzhong Shou Yuping Dai Jianyong Shao Ye Tian Shizhe Hong Tao Xu Chuize Kong Zefeng Kang Yuexin Liu Xun Qu Benkang Shi Shaobin Zheng Yi Lin Shujie Xia Dong Wei Jianbo Wu Weiling Fu Zhiping Wang Jianbo Liang | 2019 | Asian Journal of Urology2019，6，1： | 2 |
| 17 | The selective catalytic reduction of NO over Ce_(0.3)TiO_x^-supported metal oxide catalysts显示文摘A Ce_(0.3)TiO_xoxide carrier was synthesized via a sol–gel process,and Ce_(0.3)TiO_xsupported metal(M=Cd,Mn,Fe,W,Mo)oxide catalysts were prepared by the method of incipient-wetness impregnation.The catalysts were characterized by means of X-ray diffraction(XRD),Brunauer–Emmett–Teller(BET)analysis,scanning electron microscopy(SEM),transmission electron microscopy(TEM),Fourier transform infrared(FT–IR)spectroscopy,UV–Visdiffusereflectancespectroscopy(UV–VisDRS),and Temperature-programmed reduction with H_2(H_2-TPR).The catalytic activities for de-NO_(x )were evaluated by the NH_3-SCR reaction.Among all the catalysts tested,the 2 wt.%Cd/Ce_(0.3)TiO_xcatalyst exhibited the best NH_3-SCR performance,with a wide temperature window of 250–450°C for NO conversion above 90%.Moreover,the catalyst showed N_2 selectivity greater than 99%from 200 to 450°C. | Zhichen Duan Jian Liu Juan Shi Zhen Zhao Yuechang Wei Xiao Zhang Guiyuan Jiang Aijun Duan | 2018 | Journal of Environmental Sciences2018，30，3： | 2 |
| 18 | Blockade of Tim‐3 signaling restores the virus‐specific CD8+ T‐cell response in patients with chronic hepatitis B显示文摘 | WeiWu YuShi ShupingLi YunZhang YanningLiu YihuaWu ZhiChen | 2012 | Eur. J. Immunol2012，，5： | 1 |
| 19 | Baicalin inhibits hypoxia-induced pulmonary artery smooth mus- cle cell proliferation via the AKT/HIF-1 ot/p27-associat- ed pathway 显示文摘 | Zhang Lin Pu Zhichen Wang Junsong | 2014 | Int J Mol Sci2014，15，5： | 1 |
| 20 | PeerSearch: Efficient Information Retrieval in Peer-to-Peer Networks 显示文摘 | Chunqiang Tang Zhichen Xu Mallik Mahalingam | 2003 | ACM SIGCOMM Computer Communication Review2003，33，1： | 1 |
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