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| 1 | Down-regulation of Hsp90 could change cell cycle distribution and increase drug sensitivity of tumor cells显示文摘:AIM To construct Hsp90 antisense RNAeukaryotic expression vector, transfect it intoSGC7901 and SGC7901/VCR of MDR-type humangastric cancer cell lines, HCC7402 of humanhepatic cancer and Eel09 of human esophagealcancer cell lines, and to study the cell cycledistribution of the gene transected cells andtheir response to chemotherapeutic drugs.METHODS A I .03kb cDNA sequence of Hsp90Pwas obtained from the primary plasmid phHsp90by EcoR 1 and BamH I nuclease digestion andwas cloned to the EcoR 1 and BamH 1 site ofthe pcDNA by T4DNA ligase and an antisenseorientation of Hsp900 expression vector wasconstructed. The constructs were transfectedwith lipofectamine and positive clones wereselected with G418. The expression of RNA wasdetermined with dot blotting and RNaseprotection assay, and the expression of Hsp90protein determined with Western blot. Cell cycledistribution of the transfectants was analyzedwith flow cytometry, and the drug sensitivity ofthe transfectants to adriamycin (ADR ),vincrinstine (VCR ), mitomycin (MMC ) andcyclophosphamide (CTX ) with MTT andintracellular drug concentration of thetransfectants was determined with flowcytometry.RESULTS In EcoR 1 and BamH I restrictionanalysis, the size and the direction of the clonedsequence of Hsp900 remained what had beendesigned and the gene constructs were namedpcDNA-Hsp90. AH^SGC7901, AH^SGC7901/ VCR,AH-HCC7402 and AH-Eel09 cell clones allexpressed Hsp90 anti--sense RNA. Theexpression of Hsp90 was down--regulated in AHSGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH--Eel09 cell clones. Cell cycle distribution waschanged differently. In AH-SGC7901/ VCR andAH-Ec109 cells, G, phase cells were increased; Sphase and G, phase cells were decreased ascompared with their parental cell lines. In AHSGC7901 cell, G, phase cells were decreased, Qphase cells increased and S phase cells were notchanged, and in AH-HCC7402 cells G,, S and qphase cells remained unchanged as comparedwith their parental cell lines. The sensitivity ofAH--SGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH-Ec109 to chemotherapeutic drugs, thesensitivity ot AH--SGC7901/ VCR to ADR, VCR,MMC and CTX the sensitivity of AH-HCC7402 toADR and VCR, and the sensitivity of Eel09 toADR, VCR and CTX all increased as comparedwith their parental cell lines. The meanfluorescence intensity of ADR in AH--SGC7901,AH-SGC7901/ VCR, AH--HCC7402 and AH-Ec109was also significantly elevated (P< 0. 05).CONCLUSION Down-regulation of HsP90 couldchange cell cycle distribution and increase thedrug sensitivity of tumor cells. | Liu XL Xiao B Yu ZC Guo JC Zhao QC Xu L Shi YQ Fan DM | 1999 | World Journal of Gastroenterology1999，5，3： | 21 |
| 2 | Expression of co-stimulator 4-1BB molecule in hepatocellular carcinoma and adjacent non-tumor liver tissue,and its possible role in tumor immunity显示文摘AIM:To investigate the expression of 4-1BB molecule in hepatocellular carcinoma (HCC) and its adjacent tissues.METHODS:Reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the gene expression of 4-1BB in hepatocarcinoma and its adjacent tissues,and peripheral blood mononuclear cells (PBMCs) from both HCC and health control groups.Flow cytometry was used to analyse the phenotypes of T cell subsets from the blood of HCC patients and healthy volunteers,and further to determine whether 4-1BB molecules were also expressed on the surface of CD4+ and CD8+ T cells. The localization of 4-1BB proteins on tumor infiltrating T cells was determined by direct immunofluorescence cytochemical staining and detected by confocal microscopy.RESULTS:4-1BB mRNA, which was not detectable in normal liver,was found in 19 liver tissues adjacent to tumor edge(<1.0cm).Low expression of 4-1BB mRNA was shown in 8 tumor tissues and 6 liver tissues located within 1 to 5cm away from tumor edge. In PBMCs, 4-1BB mRNA was almost not detected. Percentage of CD4^+, CD8^+ and CD3^+/CD25^+ T cells, as well as ratio of CD4 to CD8 revealed no difference between groups (P>0.05,respectively),while a significant lower percentage of CD3^+ T cell was found in HCC group as compared to healthy control group (P<0.05).However, 4-1BB molecules were almost not found on the surface of CD4+ and CD8+ T cells in HCC and healthy control group.Double-staining of 4-1BB^+/CD4^+ and 4-1BB^+/CD8^+ immunofluorescence on tumor infiltrating T cells was detected in 13 liver tissues adjacent to tumor edge (<1.0cm) by confocal microscopy.CONCLUSION: Although HCC may escape from immune attack by weak immunogenicity or downregulated expression of MHC-1 molecules on the tumor cell surface,tumor infiltrating T cells can be activated via other costimulatory signal pathways to exert a limited antitumor effect on local microenvironment. The present study also implicates that modulating 4-1BB/4-1BBL costimulatory pathway may be an effective immunotherapy strategy to augment the host response. | Wan, YL Zheng, SS Zhao, ZC Li, MW Jia, CK Zhang, H | 2004 | World Journal of Gastroenterology2004，10，2： | 6 |
| 3 | Intraspinal transplantation of CD34+ human umbilical cord blood cells after spinal cord hemisection injury improves functional recovery in adult rats显示文摘 | Zhao,ZM Li,HJ Liu,HY Lu,SH Yang,RC Zhang,QJ Han,ZC | 2005 | 中国生物学文摘2005，19，3： | 2 |
| 4 | Chlamydia pneumoniae negatively reg- ulates ABCA1 expression via TLR2-Nuclear factor-kappa B and miR- 33 pathways in THP-1 macrophage-derived foam cells显示文摘 | Zhao GJ Mo ZC Tang SL | 2014 | Atherosclero- sis2014，235，2： | 1 |
| 5 | Improvement of chromosomal preparation from peripheral blood in newborn hyperbilirubinemia显示文摘 | Zhu XY Zhao R Ye ZC | 2006 | Laboratory Medicinel and Clinic2006，2，3： | 1 |
| 6 | Tannins and other phenolics from Myrica esculenta bark显示文摘 | Sun DW Zhao ZC Herbet W | 1988 | Phytochemistry1988，27，2： | 1 |
| 7 | Mechanistic insights into diabetes mellitus and oxidative stress显示文摘 | Kenneth M Zhao ZC Yang CS | 2007 | Curr Med Chem2007，14，16： | 1 |
| 8 | Identification of transcription factor KLF8 as a downstream target of focal adhesion kinase in its regu- lation of cyclin D1 and cell cycle progression显示文摘 | Zhao J Bian ZC Yee K | 2003 | Mol Cell2003，11，： | 1 |
| 9 | Detection of prostate cancer with three-dimensional transrectal ultrasound:correlation with biopsy results显示文摘 | Zhao HX Zhu Q Wang ZC | 2011 | Br J Radiol2011，84，28： | 1 |
| 10 | Detection of prostate cancer with three dimensional transrectal ultrasound:correlation with biopsy results显示文摘 | Zhao HX Zhu Q Wang ZC | 2012 | British Journal of Radiology2012，85，： | 1 |
| 11 | High prevalence of metallo-β-lactamase among carbapenem-resistant Klebsiella pneumoniae in a teaching hospital in China显示文摘 | Li B Xu XH Zhao ZC | 2014 | Can J Microbiol2014，60，10： | 1 |
| 12 | Erythropoietin is a novel vascular protectant through activation of aktl and mitochondrial modulation of cysteine proteases显示文摘 | Zhao ZC Jing QK Kenneth M | 2002 | Circulation2002，106，23： | 1 |
| 13 | Demonstration of vasculogenic mim- icry in astrocytomas and effects of Endostar on U251 cells 显示文摘 | Liu ZC Li Y Zhao W | 2011 | Pathol Res Pract2011，207，10： | 1 |
| 14 | Expression of Pancreatic Islet MHC Class I, Insulin, and ICA_ 512 Tyrosine Phosphatase in Low-dose Streptozotoein-induced Diabetes in Mice 显示文摘 | Li ZC Zhao Ij Sandier S | 2000 | J Histochem Cyto- chem2000，48，6： | 1 |
| 15 | Construction of recombinant lentiviral vectors for mouse NMNAT1 gene expression and its interference RNA显示文摘 | Zhao H Yang ZC Zhang JY | 2011 | Zhejiang Da Xue Xue Bao Yi Xue Ban2011，40，： | 1 |
| 16 | The synthesis and characterization of TiO2/wollastonite composite 显示文摘 | Zhao JZ Wang ZC Wang LW Yang H Zhao MY | 1998 | Materials Letters1998，37，： | 1 |
| 17 | Expression of human cardiac - specific homeobox protein in Escherichia coli显示文摘 | Zhao JH Xu Z Hua ZC | 2000 | Protein Expr Purif2000，18，3： | 1 |
| 18 | The biomeehanies of point contact dynamic compression plate and its effects on bone perfusion 显示文摘 | Zhao YF Li QH Gu ZC | 2006 | Chin J Traumatol2006，9，3： | 1 |
| 19 | The Tbermodynamic Performance of a new solution cycle in double absorption beat transformer using wa- ter/lithium bromide as the work -ing fluids显示文摘 | Zhao ZC Zhou FW Zhang XD | 2003 | International Journal of Refrigeration2003，26，3： | 1 |
| 20 | Hematopoiesis capacity,immunomodulatory effect and ex vivo expansion potential of mesenchymal stem cells are not impaired by cryopreservation显示文摘 | Zhao ZG Li WM Chen ZC | | 0，，04： | 1 |
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