|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | RNA-Seq analysis of yak ovary: improving yak gene structure information and mining reproduction-related genes显示文摘RNA-Seq,a high-throughput(HT)sequencing technique,has been used effectively in large-scale transcriptomic studies,and is particularly useful for improving gene structure information and mining of new genes.In this study,RNA-Seq HT technology was employed to analyze the transcriptome of yak ovary.After Illumina-Solexa deep sequencing,26826516 clean reads with a total of 4828772880 bp were obtained from the ovary library.Alignment analysis showed that 16992 yak genes mapped to the yak genome and 3734 of these genes were involved in alternative splicing.Gene structure refinement analysis showed that7340 genes that were annotated in the yak genome could be extended at the 5′or 3′ends based on the alignments been the transcripts and the genome sequence.Novel transcript prediction analysis identified 6321 new transcripts with lengths ranging from 180 to 14884 bp,and 2267 of them were predicted to code proteins.BLAST analysis of the new transcripts showed that1200?4933 mapped to the non-redundant(nr),nucleotide(nt)and/or SwissProt sequence databases.Comparative statistical analysis of the new mapped transcripts showed that the majority of them were similar to genes in Bos taurus(41.4%),Bos grunniens mutus(33.0%),Ovis aries(6.3%),Homo sapiens(2.8%),Mus musculus(1.6%)and other species.Functional analysis showed that these expressed genes were involved in various Gene Ontology(GO)categories and Kyoto Encyclopedia of Genes and Genomes pathways.GO analysis of the new transcripts found that the largest proportion of them was associated with reproduction.The results of this study will provide a basis for describing the normal transcriptome map of yak ovary and for future studies on yak breeding performance.Moreover,the results confirmed that RNA-Seq HT technology is highly advantageous in improving gene structure information and mining of new genes,as well as in providing valuable data to expand the yak genome information. | LAN DaoLiang XIONG XianRong WEI YanLi XU Tong ZHONG JinCheng ZHI XiangDong WANG Yong LI Jian | 2014 | Science China(Life Sciences)2014，57，9： | 23 |
| 2 | One-step synthesis of novel snowflake-like Si-O/Si-C nanostructures on 3D graphene/Cu foam by chemical vapor deposition显示文摘合成进程为的最近的开发三维(3D )基于 graphene 的结构趋于集中于多孔的 nanostructures 的连续改进,为造 3D 体系结构在薄电影的制造,和机制期间做修正。这里,我们综合了由一步舞的 3D graphene/Cu 泡沫上的新奇像雪花的 Si-O/Si-C nanostructures 低压的化学蒸汽免职(CVD ) 。通过系统的 micromorphological 描述, nanostructures 的形成机制包含了融化 Cu 泡沫表面和产生蒸汽的随后的冷凝作用,这被决定,通过催化作用的 graphene 的 3D 生长面对 Cu ,并且最后,在充满碳的空气的 Si-O/Si-C nanostructure 的成核。因此由调节生长温度和持续时间,与精确控制如此的像雪花的 nanostructures 的成核和进化应该是可能的。电气化学的大小显示像雪花的 nanostructures 为精力存储作为材料显示出优秀性能。Si-O/Si-C nanostructures 的最高特定的电容是以 1 mV/s 的扫描率的 963.2 mF/cm 2 。甚至在 20,000 个顺序的周期以后,进一步,电极保留了 94.4% 它的电容。 | Jing Ning Dong Wang Jincheng Zhang Xin Feng Ruixia Zhong Jiabo Chen Jianguo Dong Lixin Guo Yue Hao | 2018 | Nano Research2018，11，4： | 2 |
| 3 | Molecular cloning and characterization of a defender gainst cell death 1 gene homologue in Yak (Bos grunniens) 显示文摘 | Wang Li Zi Xiangdong Zhong Jincheng | 2010 | Journal of Applied Animal Research2010，38，2： | 1 |
| 4 | In vivo label-free measurement of blood flow velocity symmetry based on dual line scanning third-harmonic generation microscopy excited at the 1700 nm window显示文摘Measurement of bloodflow velocity is key to understanding physiology and pathology in vivo.While most measurements are performed at the middle of the blood vessel,little research has been done on characterizing the instantaneous bloodflow velocity distribution.This is mainly due to the lack of measurement technology with high spatial and temporal resolution.Here,we tackle this problem with our recently developed dual-wavelength line-scan third-harmonic generation(THG)imaging technology.Simultaneous acquisition of dual-wavelength THG line-scanning signals enables measurement of bloodflow velocities at two radially symmetric positions in both venules and arterioles in mouse brain in vivo.Our results clearly show that the instantaneous bloodflow velocity is not symmetric under general conditions. | Hui Cheng Jincheng Zhong Ping Qiu Ke Wang | 2024 | Journal of Innovative Optical Health Sciences2024，17，1： | 0 |
| 5 | 基因组和转录组测序整合分析揭示混合型肝癌的不同分子亚型显示文摘文章简介原发性肝癌每年造成全球约70万病人死亡且过半死亡病例来自中国,其致死率在所有癌种中高居第二。肝癌主要分为3种类型:肝细胞癌(约80%)、肝内胆管癌(约15%)和混合型肝癌(约5%)。混合型肝癌兼具肝细胞癌和胆管细胞癌两种成分,是预后最差的一种肝癌。 | 薛瑞栋 陈璐 张冲 藤田征志 Ruoyan Li Shu-Mei Yan Choon Kiat Ong Xiwen Liao Qiang Gao Shota Sasagawa Yanmeng Li Jincheng Wang Hua Guo Qi-Tao Huang Qian Zhong Jing Tan Lisha Qi Wenchen Gong Zhixian Hong Meng Li Jingmin Zhao Tao Peng Yinying Lu Kiat Hon Tony Lim Arnoud Boot Atushi Ono Kazuaki Chayama Zemin Zhang Steve George Rozen Bin Tean Teh Xin Wei Wang Hidewaki Nakagawa 曾木圣 白凡 张宁 | 2020 | 科学新闻2020，，2： | 0 |
| 6 | Mechanism of size effects of a filler on the wear behavior of ultrahigh molecular weight polyethylene显示文摘Although the size effects of a filler are closely related to the complex multi-level structures of their polymer composites;unfortunately,such relationships remain poorly understood.In this study,we investigated the effects of various sizes(40-600 nm)of silicon carbide(SiC)fillers on the wear behavior of ultrahigh molecular weight polyethylene(UHMWPE)in the presence of the silane coupling agent KH-560.All of these SiC fillers improved the wear resistance of UHMWPE significantly,with a medium size(150 nm)being optimal.To examine the reasons for this behavior,we analyzed the multi-level structures of the samples in terms of their matrix structures(crystalline;amorphous;interphase),matrix-filler interactions(physical adsorption;chemical crosslinking;hybrid network)and the external effects of SiC fillers(bearing loads;transferring frictional heat).The high rigidity and thermal conductivity of SiC fillers and,more importantly,the intrinsic characteristics of the matrix structures(larger crystal grains;higher interphase;stronger amorphous entangled networks)were the key parameters affecting the enhancement in the wear-resistance of the UHMWPE.Herein,we also provide interpretations of the corresponding physical effects.Our results should improve our understanding of the structure-property relationships and,thus,should guide the formula design of UHMWPE composites. | Huan Zhang Shicheng Zhao Zhong Xin Chunlin Ye Zhi Li Jincheng Xia Jiaorong Li | 2020 | Chinese Journal of Chemical Engineering2020，28，7： | 0 |
| 7 | Population genome of the newly discovered Jinchuan yak to understand its adaptive evolution in extreme environments and generation mechanism of the multirib trait显示文摘The adaptation and diversity of animals to the extreme environments of the Qinghai–Tibet Plateau(QTP)are typical materials to study adaptive evolution.The recently discovered Jinchuan yak population has many individuals with multiple ribs.However,little is known about this yak’s origin,evolution,and the genetic mechanisms that formed its unique multirib trait.Here,we report a valuable population genome resource of the Jinchuan yak by resequencing the whole genome of 150 individuals.Population genetic polymorphism and structure analysis reveal that Jinchuan yak can be differentiated as a unique and original yak population among the domestic yak.Combined with geological change,the Jinchuan yak’s evolutionary origin is speculated to be about 6290 years ago,which may be related to the unique geographical environment of the eastern edge of the QTP during this period.Compared with other domestic yaks,this new population has 280 positively selected genes.The genes related to skeletal function hold a considerable and remarkable proportion,suggesting that the specific skeletal characteristics have been enhanced in the adaptive evolution of Jinchuan yak in the extreme plateau environment.The genome-wide association study has revealed that TUBA8 and TUBA4A,the genes that regulate the cytoskeleton,are potential genes associated with the multirib trait.Our findings provide a basis to further understand the generation mechanism of the adaptive evolution of this new population in high-altitude extreme environments and the multivertebrate trait of domestic animals. | Daoliang LAN Wenhui JI Xianrong XIONG Qiqi LIANG Wenye YAO Tserang-Donko MIPAM Jincheng ZHONG Jian LI | 2021 | Integrative Zoology2021，16，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员