5篇
您的检索式：作者名="Daoliang LAN"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | RNA-Seq analysis of yak ovary: improving yak gene structure information and mining reproduction-related genes显示文摘RNA-Seq,a high-throughput(HT)sequencing technique,has been used effectively in large-scale transcriptomic studies,and is particularly useful for improving gene structure information and mining of new genes.In this study,RNA-Seq HT technology was employed to analyze the transcriptome of yak ovary.After Illumina-Solexa deep sequencing,26826516 clean reads with a total of 4828772880 bp were obtained from the ovary library.Alignment analysis showed that 16992 yak genes mapped to the yak genome and 3734 of these genes were involved in alternative splicing.Gene structure refinement analysis showed that7340 genes that were annotated in the yak genome could be extended at the 5′or 3′ends based on the alignments been the transcripts and the genome sequence.Novel transcript prediction analysis identified 6321 new transcripts with lengths ranging from 180 to 14884 bp,and 2267 of them were predicted to code proteins.BLAST analysis of the new transcripts showed that1200?4933 mapped to the non-redundant(nr),nucleotide(nt)and/or SwissProt sequence databases.Comparative statistical analysis of the new mapped transcripts showed that the majority of them were similar to genes in Bos taurus(41.4%),Bos grunniens mutus(33.0%),Ovis aries(6.3%),Homo sapiens(2.8%),Mus musculus(1.6%)and other species.Functional analysis showed that these expressed genes were involved in various Gene Ontology(GO)categories and Kyoto Encyclopedia of Genes and Genomes pathways.GO analysis of the new transcripts found that the largest proportion of them was associated with reproduction.The results of this study will provide a basis for describing the normal transcriptome map of yak ovary and for future studies on yak breeding performance.Moreover,the results confirmed that RNA-Seq HT technology is highly advantageous in improving gene structure information and mining of new genes,as well as in providing valuable data to expand the yak genome information. | LAN DaoLiang XIONG XianRong WEI YanLi XU Tong ZHONG JinCheng ZHI XiangDong WANG Yong LI Jian | 2014 | Science China(Life Sciences)2014，57，9： | 23 |
| 2 | Genomic Characterization and High Prevalence of Bovaviruses in Swine 显示文摘 | Shan Tongling Lan Daoliang Li Linlin | 2011 | PLoS One2011，6，17： | 1 |
| 3 | Molecular characterization and phylogenetic analysis of the genome of porcine torovirus显示文摘 | Huan Sun Daoliang Lan Lifang Lu Molin Chen Changsong Wang Xiuguo Hua | 2014 | Archives of Virology2014，，4： | 1 |
| 4 | Molecular characterization of a porcine kobuvirus strain in China显示文摘 | Changsong Wang Daoliang Lan Li Cui Zhibiao Yang Congli Yuan Xiuguo Hua | 2012 | Archives of Virology2012，，3： | 1 |
| 5 | Population genome of the newly discovered Jinchuan yak to understand its adaptive evolution in extreme environments and generation mechanism of the multirib trait显示文摘The adaptation and diversity of animals to the extreme environments of the Qinghai–Tibet Plateau(QTP)are typical materials to study adaptive evolution.The recently discovered Jinchuan yak population has many individuals with multiple ribs.However,little is known about this yak’s origin,evolution,and the genetic mechanisms that formed its unique multirib trait.Here,we report a valuable population genome resource of the Jinchuan yak by resequencing the whole genome of 150 individuals.Population genetic polymorphism and structure analysis reveal that Jinchuan yak can be differentiated as a unique and original yak population among the domestic yak.Combined with geological change,the Jinchuan yak’s evolutionary origin is speculated to be about 6290 years ago,which may be related to the unique geographical environment of the eastern edge of the QTP during this period.Compared with other domestic yaks,this new population has 280 positively selected genes.The genes related to skeletal function hold a considerable and remarkable proportion,suggesting that the specific skeletal characteristics have been enhanced in the adaptive evolution of Jinchuan yak in the extreme plateau environment.The genome-wide association study has revealed that TUBA8 and TUBA4A,the genes that regulate the cytoskeleton,are potential genes associated with the multirib trait.Our findings provide a basis to further understand the generation mechanism of the adaptive evolution of this new population in high-altitude extreme environments and the multivertebrate trait of domestic animals. | Daoliang LAN Wenhui JI Xianrong XIONG Qiqi LIANG Wenye YAO Tserang-Donko MIPAM Jincheng ZHONG Jian LI | 2021 | Integrative Zoology2021，16，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员