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| 1 | Comparative Transcriptome Analysis Reveals Different Host Cell Responses to Acute and Persistent Foot-and-Mouth Disease Virus Infection显示文摘Foot-and-mouth disease virus(FMDV) rapidly causes cytopathic effects in susceptible cells. Incomplete viral clearance during the acute infection leads to persistent infection. The relationship between host gene expression and the persistent infection remains unclear. In this study, we analyzed the transcriptome profiles of BHK-21 cells acutely and persistently infected with FMDV to identify differences in gene expression. GO and KEGG enrichment analyses showed that the 8,378 differentially expressed genes were significantly enriched in categories including metabolism, biosynthesis, ribosome function, and endocytosis. In persistently infected BHK-21 cells, ribosome-and translation-related genes were significantly down-regulated. There were more differentially expressed immune-related genes during persistent infection than during acute infection. Two hundred and seventy-four genes were differentially expressed in both acutely and persistently infected BHK-21 cells. Among these genes, heat shock protein family B member 1(Hspb1) knockdown significantly inhibited FMDV replication. Our research provides a basis for further research to understand the mechanisms of persistent FMDV infection including the genes involved in FMDV replication. | Jiadai Li Lingling Han Yao Hao Yuncong Yuan Mingzhen Wang Xiu Xin Hailong Wang Fang Yu Congyi Zheng Chao Shen | 2020 | Virologica Sinica2020，35，1： | 4 |
| 2 | Indoleamine 2,3-dioxygenase and regulatory dendritic cells contribute to the allograft protection induced by infusion of donor-specific splenic stromal cells显示文摘It has been reported that splenic stromal cells(SSCs)are capable of directly supporting the development of CD11c ^(lo)CD45RB^(+) IL-10-producing dendritic cells(DCs)from lineage-negative c-kit^(+) progenitor cells in the absence of exogenous cytokines.In vitro,DCs that differentiate on stromal cells suppress mixed leukocyte reaction responses and induce primary alloreactive CD4^(+) T cells to differentiate into IL-10-producing Tr1 cells.However,the precise mechanisms by which these SSCs exert their regulatory functions in vivo remain undefined.Furthermore,their possible contribution to the development of allograft transplantation tolerance has yet to be examined.Here,we have used both murine skin and cardiac allograft transplantation models to explore whether in vivo alloresponses can be regulated by infusion with donor-derived SSCs and to investigate the possible mechanisms by which SSCs exert regulatory effects to prevent allograft rejection.We show that intravenous SSC infusion prolonged murine skin allograft survival.The prolonged graft survival is associated with augmentation of the generation of regulatory DC subsets and CD4^(+) CD25^(+) Foxp3^(+) regulatory T cells(Tregs),as well as upregulation of the production of suppressive cytokines IL-10 and transforming growth factor(TGF)-b.Moreover,we found that indoleamine 2,3-dioxygenase and SSC-derived regulatory DCs contribute to allograft protection by infusion of donor-specific SSCs.Our data suggest that donor-derived SSCs could be used as a therapeutic target to promote transplantation tolerance. | Li Liu Lihua Duan Min Gong Hong Dai Quan Gong Fang Zheng Zheng Tan Congyi Wang Feili Gong Min Fang | 2011 | Cellular & Molecular Immunology2011，8，1： | 2 |
| 3 | Cryopreservation of Pollen by Vitrification in Brassica显示文摘CryopreservationofPolenbyVitrificationinBrasicaXuBingfang,HanHongmei,ZhengCongyi,SunMengxiangColegeofLifeSciences,WuhanUnive... | Xu Bingfang Han Hongmei Zheng Congyi Sun Mengxiang | 1997 | Wuhan University Journal of Natural Sciences1997，2，1： | 2 |
| 4 | Construction of the Vero cell culture system that can produce infectious HCV particles显示文摘 | Jia Guo Ran Yan Guodong Xu Weiyun Li Congyi Zheng | 2009 | Molecular Biology Reports2009，，1： | 1 |
| 5 | Construction of cytopathic PK-15 cell model of classical swine fever virus显示文摘No cytopathic effect (CPE) can be observed on classical swine fever virus (CSFV) infected cell culture in vitro. This brings an obstacle to the researches on reciprocity between CSFV and host cells. Based on the construction of full-length genomic infectious Cdna clone of Chinese CSFV standard virulent Shimen strain, partial deletion is intro- duced into genomic Cdna to obtain a 7.5 kb subgenomic Cdna. A new subgenomic CSFV is derived from transfection with the subgenomic Cdna on PK-15 cells pre-infected by CSFV Shimen virus. Typical CPE induced by this subgenomic virus is observed on PK-15 cells. Coexistence of wild- type and subgenomic virus in cytopathic cell culture is dem- onstrated by RT-PCR detection in cytopathic cells. For conclusion, the construction of cytopathic cell model exploited a new way for researches on the molecular mechanism of CSFV pathogenesis. | WU Haixiang, ZHANG Chuyu, ZHENG Congyi, WANG Jiafu, PAN Zishu, LI Lei, CAO Shen & Yl GuanghuiInstitute of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaCorrespondence should be addressed to Zhang Chuyu (e-mail: avlab@ whu.edu.cn) | 2003 | Chinese Science Bulletin2003，48，9： | 1 |
| 6 | Structure-function analysis of mutant RNA-dependent RNA polymerase complexes with VPg显示文摘 | Chaojiang Gu Tao Zeng Yong Li Zhenghui Xu Zhongxi Mo Congyi Zheng | 2009 | Biochemistry (Moscow)2009，，10： | 1 |
| 7 | A Personalized Microbe Biodiversity Dataspace Information System显示文摘It can not provide dynamic view mechanism in previ-ous dataspace information system. In this paper,the dynamic ex-tendable view mechanism provided by object deputy model is proposed for personalized dataspace information system which can provide rich semantics and enough flexibility. The flexible inheritance avoids a lot of data redundancy. The cross class query mechanism allows users to find more related data based on com-plex relationships. The personalized dataspace service provides less storage space consumption and shorter query response time. The experiment result shows that our approach is more feasible and efficient than the traditional one. | LIU Junqiang PENG Zhiyong LI Xuhui ZHENG Congyi FANG Chengxiang | 2007 | Wuhan University Journal of Natural Sciences2007，12，5： | 0 |
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