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| 1 | Cryogenic 3D printing of dual-delivery scaffolds for improved bone regeneration with enhanced vascularization显示文摘Three-dimensional(3D)printing has been increasingly employed to produce advanced bone tissue engineering scaffolds with biomimetic structures and matched mechanical strengths,in order to induce improved bone regeneration in defects with a critical size.Given that the successful bone regeneration requires both excellent osteogenesis and vascularization,endowing scaffolds with both strong bone forming ability and favorable angiogenic potential would be highly desirable to induce improved bone regeneration with required vascularization.In this investigation,customized bone tissue engineering scaffolds with balanced osteoconductivity/osteoinductivity were produced via cryogenic 3D printing ofβ-tricalcium phosphate and osteogenic peptide(OP)containing water/poly(lactic-co-glycolic acid)/dichloromethane emulsion inks.The fabricated scaffolds had a hierarchically porous structure and were mechanically comparable to human cancellous bone.Angiogenic peptide(AP)containing collagen I hydrogel was then coated on scaffold surface to further provide scaffolds with angiogenic capability.A sequential release with a quick AP release and a slow but sustained OP release was obtained for the scaffolds.Both rat endothelial cells(ECs)and rat bone marrow derived mesenchymal stem cells(MSCs)showed high viability on scaffolds.Improved in vitro migration and angiogenesis of ECs were obtained for scaffolds delivered with AP while enhanced osteogenic differentiation was observed in scaffolds containing OP.The in vivo results showed that,toward scaffolds containing both AP and OP,the quick release of AP induced obvious angiogenesis in vivo,while the sustained OP release significantly improved the new bone formation.This study provides a facile method to produce dual-delivery scaffolds to achieve multiple functions. | Chong Wang Jiahui Lai Kai Li Shaokui Zhu Bingheng Lu Jia Liu Yujin Tang Yen Wei | 2021 | Bioactive Materials2021，6，1： | 7 |
| 2 | Auxin response factor 6A regulates photosynthesis,sugar accumulation,and fruit development in tomato显示文摘Auxin response factors(ARFs)are involved in auxin-mediated transcriptional regulation in plants.In this study,we performed functional characterization of SlARF6A in tomato.SlARF6A is located in the nucleus and exhibits transcriptional activator activity.Overexpression of SlARF6A increased chlorophyll contents in the fruits and leaves of tomato plants,whereas downregulation of SlARF6A decreased chlorophyll contents compared with those of wild-type(WT)plants.Analysis of chloroplasts using transmission electron microscopy indicated increased sizes of chloroplasts in SlARF6A-overexpressing plants and decreased numbers of chloroplasts in SlARF6A-downregulated plants.Overexpression of SlARF6A increased the photosynthesis rate and accumulation of starch and soluble sugars,whereas knockdown of SlARF6A resulted in opposite phenotypes in tomato leaves and fruits.RNA-sequence analysis showed that regulation of SlARF6A expression altered the expression of genes involved in chlorophyll metabolism,photosynthesis and sugar metabolism.SlARF6A directly bound to the promoters of SlGLK1,CAB,and RbcS genes and positively regulated the expression of these genes.Overexpression of SlARF6A also inhibited fruit ripening and ethylene production,whereas downregulation of SlARF6A increased fruit ripening and ethylene production.SlARF6A directly bound to the SAMS1 promoter and negatively regulated SAMS1 expression.Taken together,these results expand our understanding of ARFs with regard to photosynthesis,sugar accumulation and fruit development and provide a potential target for genetic engineering to improve fruit nutrition in horticulture crops. | Yujin Yuan Xin Xu Zehao Gong Yuwei Tang Mengbo Wu Fang Yan Xiaolan Zhang Qian Zhang Fengqing Yang Xiaowei Hu Qichen Yang Yingqing Luo Lihua Mei Wenfa Zhang Cai-Zhong Jiang Wangjin Lu Zhengguo Li Wei Deng | 2019 | Horticulture Research2019，6，1： | 3 |
| 3 | Effect of cytotoxic T-lymphocyte antigen-4,TNF-alpha polymorphisms on osteosarcoma: evidences from a meta-analysis显示文摘Objective:Previous studies have investigated the role of cytotoxic T-lymphocyte antigen-4(CTLA-4)and tumor necrosis factor-alpha(TNF-α)in carcinogenesis of osteosarcoma,but their results were inconsistent.We aimed to clarify the associations between CTLA-4,TNF-αpolymorphism and osteosarcoma risk by using meta-analysis.Methods:We searched relevant studies without language restriction in PubMed,EMbase,Cochrane Library,Google Scholar databases,Chinese National Knowledge Infrastructure(CNKI)and conference literature in humans published prior to March 2013.The strengths of the associations between genetic variants and osteosarcoma risk were estimated by odds ratio(OR)with 95%confidence interval(95%CI).Results:A total of seven studies with 1,198 osteosarcoma patients and 1,493 controls were selected.Four studies were eligible for CTLA-4(1,003 osteosarcoma and 1,162 controls),and three studies for TNF-α(195 osteosarcoma and 331 controls).Pooled results showed that rs231775 polymorphism of CTLA-4 was associated with osteosarcoma risk(GG vs.AA:OR=1.63,95%CI=1.24-2.13;GG+GA vs.AA:OR=1.56,95%CI=1.21-2.01;AA+GA vs.GG:OR=0.83,95%CI=0.71-0.97;G vs.A:OR=1.21,95%CI=1.08-1.36).No significant heterogeneity was observed across the studies.No significant associations were found between rs5742909 polymorphism of CTLA-4 or rs1800629 polymorphism of TNF-αand osteosarcoma risk.Conclusions:These results suggest that the rs231775 polymorphism of CTLA-4 may play an important role in carcinogenesis of osteosarcoma. | Jianwei Liu Junli Wang Weiping Jiang Yujin Tang | 2013 | Chinese Journal of Cancer Research2013，25，6： | 3 |
| 4 | Desiccation and cracking behaviour of clay layer from slurry state under wetting-drying cycles显示文摘 | Tang Chaosheng Cui Yujin Shi Bin | 2011 | Geoderma2011，166，1： | 1 |
| 5 | Damping of aluminum-matrix composite reinforced by carbon nanotube:Multiscale modeling and characteristics显示文摘It is a crucial requirement for structure-damping materials to attain both stiffness and damping;unfortunately,the two properties are usually mutually exclusive.This study interestingly demonstrates that introducing Ni atoms into the interface of carbon nanotube(CNT)reinforced aluminum-matrix composites can defeat the conflict of stiffness versus damping.This originates from the gradient variation of the modulus and energy dissipation in the effective interfacial zone.The rule of mixture is modified by taking the interface contribution into account,and a gradient damping model is proposed to account for the contribution of the interface energy dissipation.Molecular dynamics simulations confirm that the proposed multiscale modulus and damping models can describe the elastic modulus and damping behavior of the composites with different volume fractions and different diameters of CNTS.The gradient interface slip caused by the lattice mismatches and misfit dislocations between Ni-coated CNT and aluminum is one of the pathways for achieving unprecedented levels of the product of stiffness and damping. | WANG Fei LI Li TANG HaiShan WANG XueLin HU YuJin | 2023 | Science China(Technological Sciences)2023，66，4： | 1 |
| 6 | Impact resistance performance and optimization of the sand-EPE composite cushion in rock sheds显示文摘Rock sheds are widely used to prevent rockfall disasters along roads in mountainous areas.To improve the capacity of rock sheds for resisting rockfall impact,a sand and expandable polyethylene(EPE)composite cushion was proposed.A series of model experiments of rockfall impact on rock sheds were conducted,and the buried depth of the EPE foam board in the sand layer was considered.The impact load and dynamic response of the rock shed were investigated.The results show that the maximum impact load and dynamic response of the rock shed roof are all significantly less than those of the sand cushion.Moreover,as the distance between the EPE foam board and rock shed roof decreases,the maximum rockfall impact force and impact pressure gradually decrease,and the maximum displacement,acceleration and strain of the rock shed first decrease and then change little.In addition,the vibration acceleration and vertical displacement of the rock shed roof decrease from the centre to the edge and decrease faster along the longitudinal direction than that along the transverse direction.In conclusion,the buffering effect of the sand-EPE composite cushion is better than that of the pure sand cushion,and the EPE foam board at a depth of 1/3 the thickness of the sand layer is appropriate. | YU Bingxin ZHOU Xiaojun TANG Jianhui ZHANG Yujin ZHANG Yuefeng | 2024 | Journal of Mountain Science2024，21，2： | 0 |
| 7 | Insight into the role of ethylene glycol on thermodynamics and nucleation kinetics of dimethyl terephthalate in mixed solvent system显示文摘Dissolution and nucleation are two essential processes for industrial crystallization.This paper in-vestigates the effect of ethylene glycol addition on the crystallization behavior of dimethyl terephthalate(DMT)in solution.The DMT solubility in mixed solvent system(methanol-ethylene glycol)was deter-mined by isothermal satiation approach,and the solubility was associated using seven models.The model fitting results were consistent with the experimental values.Based on the results,the metastable zone width(MSZW)of DMT was detected by the polythermal approach;the modified Sangwal's theory was used to investigate the nucleation behavior,which can provide a new way of thought for better analysis of the crystallization behavior.The results demonstrated that MSZW was associated with various elements,such as cooling rate,saturation temperature and mass fraction of ethylene glycol.The addition of ethylene glycol slowed down the nucleation rate as shown by the broadening of MSZW.We derive the solid-liquid interface energy,the nucleation driving force,the critical nucleation size and the critical Gibbs free energy according to the classical nucleation theory.It is demonstrated that the nucleation driving force and the solid-liquid interface energy are dependent and jointly influence the MSZW. | Yujin Zhang Chunyan Shi Jing Tang Li Liu Dongxia Yan Qing Zhou Xingmei Lu Songyan Jia | 2024 | Particuology2024，85，2： | 0 |
| 8 | Computer vision-aided bioprinting for bone research显示文摘Bioprinting is an emerging additive manufacturing technology that has enormous potential in bone implantation and repair. The insufficient accuracy of the shape of bioprinted parts is a primary clinical barrier that prevents widespread utilization of bioprinting,especially for bone design with high-resolution requirements. During the last five years, the use of computer vision for process control has been widely practiced in the manufacturing field. Computer vision can improve the performance of bioprinting for bone research with respect to various aspects, including accuracy, resolution, and cell survival rate. Hence, computer vision plays a substantial role in addressing the current defect problem in bioprinting for bone research. In this review, recent advances in the application of computer vision in bioprinting for bone research are summarized and categorized into three groups based on different defect types: bone scaffold process control, deep learning, and cell viability models. The collection of printing parameters,data processing, and feedback of bioprinting information, which ultimately improves printing capabilities, are further discussed. We envision that computer vision may offer opportunities to accelerate bioprinting development and provide a new perception for bone research. | Changxi Liu Liqiang Wang Weijie Lu Jia Liu Chengliang Yang Chunhai Fan Qian Li Yujin Tang | 2022 | Bone Research2022，10，2： | 0 |
| 9 | Double crosslinked biomimetic composite hydrogels containing topographical cues and WAY-316606 induce neural tissue regeneration and functional recovery after spinal cord injury显示文摘Spinal cord injury(SCI)is an overwhelming and incurable disabling condition,for which increasing forms of multifunctional biomaterials are being tested,but with limited progression.The promising material should be able to fill SCI-induced cavities and direct the growth of new neurons,with effective drug loading to improve the local micro-organism environment and promote neural tissue regeneration.In this study,a double crosslinked biomimetic composite hydrogel comprised of acellularized spinal cord matrix(ASCM)and gelatin-acrylated-β-cyclodextrin-polyethene glycol diacrylate(designated G-CD-PEGDA)hydrogel,loaded with WAY-316606 to activate canonical Wnt/β-catenin signaling,and reinforced by a bundle of three-dimensionally printed aligned polycaprolactone(PCL)microfibers,was constructed.The G-CD-PEGDA component endowed the composite hydrogel with a dynamic structure with a self-healing capability which enabled cell migration,while the ASCM component promoted neural cell affinity and proliferation.The diffusion of WAY-316606 could recruit endogenous neural stem cells and improve neuronal differentiation.The aligned PCL microfibers guided neurite elongation in the longitudinal direction.Animal behavior studies further showed that the composite hydrogel could significantly recover the motor function of rats after SCI.This study provides a proficient approach to produce a multifunctional system with desirable physiological,chemical,and topographical cues for treating patients with SCI. | Xingchang Zhao Xianzhe Lu Kai Li Shiqiang Song Zhaohui Luo Chuanchuan Zheng Chengliang Yang Xiumei Wang Liqiang Wang Yujin Tang Chong Wang Jia Liu | 2023 | Bioactive Materials2023，，6： | 0 |
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