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| 1 | On the origin and continuing evolution of SARS-CoV-2显示文摘The SARS-Co V-2 epidemic started in late December 2019 in Wuhan, China, and has since impacted a large portion of China and raised major global concern. Herein, we investigated the extent of molecular divergence between SARS-CoV-2 and other related coronaviruses. Although we found only 4% variability in genomic nucleotides between SARS-CoV-2 and a bat SARS-related coronavirus(SARSr-CoV;Ra TG13), the difference at neutral sites was 17%, suggesting the divergence between the two viruses is much larger than previously estimated. Our results suggest that the development of new variations in functional sites in the receptor-binding domain(RBD) of the spike seen in SARS-Co V-2 and viruses from pangolin SARSr-Co Vs are likely caused by natural selection besides recombination. Population genetic analyses of 103 SARS-CoV-2 genomes indicated that these viruses had two major lineages(designated L and S), that are well defined by two different SNPs that show nearly complete linkage across the viral strains sequenced to date. We found that L lineage was more prevalent than the S lineage within the limited patient samples we examined. The implication of these evolutionary changes on disease etiology remains unclear.These findings strongly underscores the urgent need for further comprehensive studies that combine viral genomic data, with epidemiological studies of coronavirus disease 2019(COVID-19). | Xiaolu Tang Changcheng Wu Xiang Li Yuhe Song Xinmin Yao Xinkai Wu Yuange Duan Hong Zhang Yirong Wang Zhaohui Qian Jie Cui Jian Lu | 2020 | National Science Review2020，7，6： | 67 |
| 2 | In-situ fabrication of PtSe2/GaN heterojunction for self-powered deep ultraviolet photodetector with ultrahigh current on/off ratio and detectivity显示文摘The research of ultraviolet photodetectors(UV PDs)have been attracting extensive attention,due to their important applications in many areas.In this study,PtSe2/GaN heterojunction is in-situ fabricated by synthesis of large-area vertically standing two-dimensional(2D)PtSe2 film on n-GaN substrate.The PtSe2/GaN heterojunction device demonstrates excellent photoresponse properties under illumination by deep UV light of 265 nm at zero bias voltage.Further analysis reveals that a high responsivity of 193 mA·W^-1,an ultrahigh specific detectivity of 3.8 × 10^14 Jones,linear dynamic range of 155d B and current on/off ratio of^10^8,as well as fast response speeds of 45/102μs were obtained at zero bias voltage.Moreover,this device response quickly to the pulse laser of 266 nm with a rise time of 172 ns.Such high-performanee PtSe2/GaN heteroj u nction UV PD demonstrated in this work is far superior to previously reported results,suggesting that it has great potential for deep UV detection. | Ranran Zhuo Longhui Zeng Huiyu Yuan Di Wu Yuange Wang Zhifeng Shi Tingting Xu Yongtao Tian Xinjian Li Yuen Hong Tsang | 2019 | Nano Research2019，12，1： | 11 |
| 3 | Energy-Efficient Mapping for 3D NoC Using Logistic Function Based Adaptive Genetic Algorithms显示文摘The problem of mapping application tasks is one of key issues in 3D Network on chip(3D NoC) design. A novel Logistic function based adaptive genetic algorithm(LFAGA) is proposed for energy-aware mapping of homogeneous 3D NoC. We formulate the mapping problem and show the Standard genetic algorithm(SGA). The LFAGA is presented in detail with the goal of obtaining higher convergence speed while preventing the premature convergence. Experimental results indicate that the proposed LFAGA is more efficient than previously proposed Chaos-genetic mapping algorithm(CGMAP). In the experiments, a randomly generated task graph of size 27 is mapped to a 3D NoC of size 3×3×3, the convergence speed of LFAGA is 2.55 times faster than CGMAP in the best condition. When the task size increases to 64 and the 3D NoC size extends to 4×4×4, LFAGA is 2.31 times faster compared to CGMAP. For the No C sizes in the range from 3×3×2 to 4×4×4, solutions obtained by the LFAGA are consistently better than the CGMAP. For example, in the experiment of size 4×4×4, the improvement of final result reaches 30.0% in term of energy consumption. For a real application of size 3×4×2, 18.6% of energy saving can be achieved and the convergence speed is 1.58 times faster than that of the CGMAP. | WANG Jiawen LI Li WANG Zhongfeng ZHANG Rong ZHANG Yuang | 2014 | Chinese Journal of Electronics2014，23，2： | 5 |
| 4 | Fermented mixed feed alters growth performance,carcass traits,meat quality and muscle fatty acid and amino acid profiles in finishing pigs显示文摘This study was conducted to investigate the effects of fermented mixed feed(FMF)on growth performance,carcass traits,meat quality,muscle amino acid and fatty acid composition and mRNA expression levels of genes related to lipid metabolism in finishing pigs.In the present study,144 finishing pigs(Duroc×Berkshire×Jiaxing Black)were randomly allocated to 3 dietary treatments with 4 replicate pens per group and 12 pigs per pen.The dietary treatments included a basal diet(CON),a basal diet+5%FMF and a basal diet+10%FMF.The experiment lasted 38 d after 4 d of acclimation.The results showed that 5%and 10%FMF significantly increased the average daily gain(ADG)of the females but not the males(P<0.05),but FMF supplementation showed no impact on carcass traits.Moreover,10%FMF supplementation increased the meat color45 min and meat color24 h values,while it decreased the shear force relative to CON(P<0.05).In addition,10%FMF significantly increased the contents of flavor amino acids(FAA),total essential AA(EAA),total non-EAA(NEAA)and total AA relative to CON(P<0.05).Furthermore,the diet supplemented with 10%FMF significantly increased the concentration of n-3 polyunsaturated fatty acids(PUFA),n-6 PUFA and total PUFA,and the PUFA to saturated fatty acids ratio(P<0.05),suggesting that FMF supplementation increased meat quality.Moreover,compared with the CON,10%FMF supplementation increased the mRNA expression of lipogenic genes,including CEBPα,PPARγ,SREBP1 and FABP4,and upregulated the expression of unsaturated fatty acid synthesis(ACAA1 and FADS2).Together,our results suggest that 10%FMF dietary supplementation improved the female pigs’growth performance,improved the meat quality and altered the profiles of muscle fatty acids and amino acids in finishing pigs.This study provides a reference for the production of high-quality pork. | Shiqi Liu Man Du Yuang Tu Wenjing You Wentao Chen Guoliang Liu Junyue Li Yizhen Wang Zeqing Lu Tenghao Wang Tizhong Shan | 2023 | Animal Nutrition2023，，1： | 4 |
| 5 | Protein farnesylation negatively regulatesbrassinosteroid signaling via reducing BES1 stabilityinArabidopsis thaliana^(∞)显示文摘Brassinosteroids(BRs)are a group of steroidal phytohormones,playing critical roles in almost all physiological aspects during the life span of a plant.In Arabidopsis,BRs are perceived at the cell surface,triggering a reversible phosphorylation-based signaling cascade that leads to the activation and nuclear accumulation of a family of transcription factors,represented by BES1 and BZR1.Protein famesylation is a type of post-translational modification,functioning in many important cellular processes.Previous studies demonstrated a role of famesylation in BR biosynthesis via regulating the endoplasmic reticulum localization of a key bassino-lide(BL)biosynthetic enzyme BR6ox2.Whether such a process is also involved in BR signaling is not understood.Here,we demonstrate that protein famesylation is involved in mediating BR signaling in Arabidopsis.A loss-of-function mutant of EN HANCED RESPONSE TO ABA 1(iERA1).encoding a(3 subunit of the protein famesyl transferase hoi oenzyme,can alter the BL sensitivity of bak1~4 from a reduced to a hypersensitive level,era 7 can partially rescue the BR defective phenotype of a hetero zygous mutant of bin2-1,a gain-of function mutant of BIN2 which encodes a negative regulator in the BR signaling.Our genetic and biochemical analyses revealed that ERA1 plays a significant role in regulating the protein stability of BES1. | Zengxiu Feng Hongyong Shi Minghui Lv Yuang Ma Jia Li | 2021 | Journal of Integrative Plant Biology2021，63，7： | 3 |
| 6 | Land exploitation resulting in soil salinization in a desert-oasis ecotone 显示文摘 | Wang Yuang Li Yan | 2012 | Catena2012，100，1： | 1 |
| 7 | Anthropogenic flow analysis of Hefei City, China 显示文摘 | LI S S YUANG Z M BI J | 2010 | Science of the Total Environ- ment2010，408，： | 1 |
| 8 | Interoceptive regulation of skeletal tissue homeostasis and repair显示文摘Recent studies have determined that the nervous system can sense and respond to signals from skeletal tissue,a process known as skeletal interoception,which is crucial for maintaining bone homeostasis.The hypothalamus,located in the central nervous system(CNS),plays a key role in processing interoceptive signals and regulating bone homeostasis through the autonomic nervous system,neuropeptide release,and neuroendocrine mechanisms.These mechanisms control the differentiation of mesenchymal stem cells into osteoblasts(OBs),the activation of osteoclasts(OCs),and the functional activities of bone cells.Sensory nerves extensively innervate skeletal tissues,facilitating the transmission of interoceptive signals to the CNS.This review provides a comprehensive overview of current research on the generation and coordination of skeletal interoceptive signals by the CNS to maintain bone homeostasis and their potential role in pathological conditions.The findings expand our understanding of intersystem communication in bone biology and may have implications for developing novel therapeutic strategies for bone diseases. | Yao Xiao Changhao Han Yunhao Wang Xinshu Zhang Rong Bao Yuange Li Huajiang Chen Bo Hu Shen Liu | 2023 | Bone Research2023，11，3： | 1 |
| 9 | Engineering a folic acid-decorated ultrasmall gemcitabine nanocarrier for breast cancer therapy: Dual targeting of tumor cells and tumor-associated macrophages显示文摘Combination of passive targeting with active targeting is a promising approach to improve the therapeutic efficacy of nanotherapy.However,most reported polymeric systems have sizes above100 nm,which limits effective extravasation into tumors that are poorly vascularized and have dense stroma.This will,in turn,limit the overall effectiveness of the subsequent uptake by tumor cells via active targeting.In this study,we combined the passive targeting via ultra-small-sized gemcitabine(GEM)-based nanoparticles(NPs)with the active targeting provided by folic acid(FA)conjugation for enhanced dual targeted delivery to tumor cells and tumor-associated macrophages(TAMs).We developed an FAmodified prodrug carrier based on GEM(PGEM)to load doxorubicin(DOX),for co-delivery of GEM and DOX to tumors.The co-delivery system showed small particle size of~10 nm in diameter.The ligand-free and FA-targeted micelles showed comparable drug loading efficiency and a sustained DOX release profile.The FA-conjugated micelles effectively increased DOX uptake in cultured KB cancer cells that express a high level of folate receptor(FR),but no obvious increase was observed in 4T1.2 breast cancer cells that have a low-level expression of FR.Interestingly,in vivo,systemic delivery of FAPGEM/DOX led to enhanced accumulation of the NPs in tumor and drastic reduction of tumor growth in a murine 4T1.2 breast cancer model.Mechanistic study showed that 4T1.2 tumor grown in mice expressed a significantly higher level of FOLR2,which was selectively expressed on TAMs.Thus,targeting of TAM may also contribute to the improved in vivo targeted delivery and therapeutic efficacy. | Pearl Moharil Zhuoya Wan Apurva Pardeshi Jiang Li Haozhe Huang Zhangyi Luo Sanjay Rathod Ziqian Zhang Yuang Chen Bei Zhang Christian A.Fernandez Jingjing Sun Song Li | 2022 | Acta Pharmaceutica Sinica B2022，12，3： | 1 |
| 10 | Research on Overloadin-g Protection of Permanent Magnetic Coupler in Coal Mine显示文摘 | YUANG Wenqi LIU Yue LI Dong | 2014 | Applied Mechanics and Materials2014，597，： | 1 |
| 11 | A conductive supramolecular hydrogel creates ideal endogenous niches to promote spinal cord injury repair显示文摘The current effective method for treatment of spinal cord injury(SCI)is to reconstruct the biological microenvironment by filling the injured cavity area and increasing neuronal differentiation of neural stem cells(NSCs)to repair SCI.However,the method is characterized by several challenges including irregular wounds,and mechanical and electrical mismatch of the material-tissue interface.In the current study,a unique and facile agarose/gelatin/polypyrrole(Aga/Gel/PPy,AGP3)hydrogel with similar conductivity and modulus as the spinal cord was developed by altering the concentration of Aga and PPy.The gelation occurred through non-covalent interactions,and the physically crosslinked features made the AGP3 hydrogels injectable.In vitro cultures showed that AGP3 hydrogel exhibited excellent biocompatibility,and promoted differentiation of NSCs toward neurons whereas it inhibited over-proliferation of astrocytes.The in vivo implanted AGP3 hydrogel completely covered the tissue defects and reduced injured cavity areas.In vivo studies further showed that the AGP3 hydrogel provided a biocompatible microenvironment for promoting endogenous neurogenesis rather than glial fibrosis formation,resulting in significant functional recovery.RNA sequencing analysis further indicated that AGP3 hydrogel significantly modulated expression of neurogenesis-related genes through intracellular Ca2+signaling cascades.Overall,this supramolecular strategy produces AGP3 hydrogel that can be used as favorable biomaterials for SCI repair by filling the cavity and imitating the physiological properties of the spinal cord. | Biao Yang Chengzhen Liang Di Chen Feng Cheng Yuang Zhang Shaoke Wang Jiawei Shu Xianpeng Huang Jingkai Wang Kaishun Xia Liwei Ying Kesi Shi Chenggui Wang Xuhua Wang Fangcai Li Qian Zhao Qixin Chen | 2022 | Bioactive Materials2022，7，9： | 0 |
| 12 | Chemically modified DNA nanostructures for drug delivery显示文摘Based on predictable,complementary base pairing,DNA can be artificially pre-designed into versatile DNA nanostructures of well-defined shapes and sizes.With excellent addressability and biocompatibility,DNA nanostructures have been widely employed in biomedical research,such as biosensing,bio-imaging,and drug delivery.With the development of the chemical biology of nucleic acid. | Yuang Wang Xuehe Lu Xiaohui Wu Yan Li Wantao Tang Changping Yang Jianbing Liu Baoquan Ding | 2022 | The Innovation2022，3，2： | 0 |
| 13 | New paleomagnetic evidences of paleogene tectonic rotation of the Qaidam Basin and adjacent region: mechanism and its tectonic implication显示文摘In order to better understand the tectonic evolution of the Qaidam Basin. The authors carried out a paleomagnetic study on 39 samples of 7 sites from Hongsanhan section, northwest Qaidam Basin. Stepwise thermo-demagnetization isolated a high-temperature component (HTC), which passes the fold and reversal tests at 95% confidence level. It suggests that the HTC should be primary. Unblocking temperature (about 685℃) and the experiment of isothermal remanence indicate that hematite is the mainly carrier of the remanence. A tilt-corrected mean direction is: Ds=7.1°, Is=38.5°, α95=7.4°. Corresponding to a palaeopole at, φ=250.1°E, λ=72.0°N, A95=6.8°, Compared with the reference APWP of Eurasia, the sampling area occurred a non-significant rotation (3.4°±5.5°) relative to Eurasia since Eocene due to the affect of left-lateral Altyn fault, Based on the paleomagnetic results of early Cretaceous and Teritary within the Qaidam block, we can obtained the mean clockwise rotations from the samples deposited from 160 Ma to 45 Ma is 24.5°±9.0°, and from 38 Ma to present is -0.5°±7.5° relative to present geomagnetism respectively. During the Mid Eocene (45~38 Ma) rotation phase, there should existing an important early tectonic event in northern part of the present-day Tibetan Plateau, which probably represents one of large-scale strike-slip events of the Altyn strike-slip fault. | Weimin Li Yongjiang Liu Zhiming Sun Xiaohong Ge Shoumai Ren Junling Pei Sihua Yuang Xinzhuan Guo | 2006 | Global Geology2006，9，1： | 0 |
| 14 | Study on Analyzing Monodisperse Uranium Oxide Particle by FT-TIMS显示文摘Environmental sampling is the important one of IAEA safeguards technology, the aim of which is detecting the undeclared nuclear activities. Analyzing isotopic ratio of single uranium-bearing particle in swipe samples was a effective analytic technique in virtue of its ability of achieving the present or past information of nuclear facilities. For this purpose, a new method of Fission track (FT) technique combined with thermal ionization mass spectrometry (TIMS) was developed. | CHEN Yan WANG Fan ZHAO Yong-gang LI Li-li ZHANG Yan SHEN Yan CUI Jian-yong LIU Yuang | 2012 | Annual Report of China Institute of Atomic Energy2012，，1： | 0 |
| 15 | From the teapot effect to tap-triggered self-wetting: a 3D self-driving sieve for whole blood filtration显示文摘Achieving passive microparticle filtration with micropore membranes is challenging due to the capillary pinning effect of the membranes.Inspired by the teapot effect that occurs when liquid(tea)is poured from a teapot spout,we proposed a tap-triggered self-wetting strategy and utilized the method with a 3D sieve to flter rare cells.First,a 3D-printed polymer tap-trigger microstructure was implemented.As a result,the 3μm micropore membrane gating threshold(the pressure needed to open the micropores)was lowered from above 3000 to 80 Pa by the tap-trigger microstructure that facilated the liquid leakage and spreading to self-wet more membrane area in a positive feedback loop.Then,we implemented a 3D cone-shaped cell sieve with tap-trigger microstructures.Driven by gravity,the sieve performed at a high throughput above 20 mL/min(DPBS),while the micropore size and porosity were 3μm and 14.1%,respectively.We further filtered leukocytes from whole blood samples with the proposed new 3D sieve,and the method was compared with the traditional method of leukocyte isolation by chemically removing red blood cells.The device exhibited comparable leukocyte purity but a higher platelet removal rate and lower leukocyte simulation level,facilitating downstream single-cell analysis.The key results indicated that the tap-triggered self-wetting strategy could significantly improve the performance of passive microparticle filtration. | Yuang Li Xue Li Lina Zhang Xiaofeng Luan Jiahong jiang Lingqian Zhang Mingxiao Li Jinghui Wang Jiangang Duan Haiping Zhao Yang Zhao Chengjun Huang | 2023 | Microsystems & Nanoengineering2023，9，2： | 0 |
| 16 | Steady-State Visual Evoked Potential (SSVEP) in a New Paradigm Containing Dynamic Fixation Points显示文摘Steady-state visual evoked potential(SSVEP)has become a powerful tool for Brain Computer Interface(BCI)because of its high signal-tonoise ratio,high information transmission rate,and minimal user training.At present,the edge information of each region cannot be identified in spatial coding based on SSVEP-BCI technology,and the user experience is poor.To solve this problem,this paper designed a new paradigm to explore the relationship between the fixation point position of continuous sliding and the correlation coefficient ratio in the dualfrequency case.Firstly,the standard sinusoidal signal was employed to simulate the Electroencephalogram(EEG)signal,which verified the reliability of characterizing the amplitude variation of test signal by correlation coefficient.Then,the relationship between the amplitude response of SSVEP and the distance between the fixation point and the stimulus in the horizontal direction was tested by Canonical Correlation Analysis(CCA)and Filter bank CCA(FBCCA).Finally,the experimental data were offline analyzed under the condition of continuous sliding of the fixation point.It is feasible and reasonable to detect the amplitude change of frequency component in SSVEP by utilizing the spatial coding method in this paper to improve the extraction accuracy of spatial information. | Yuang Li Yong Ge Xuefei Zhong Xiong Zhang | 2022 | China Communications2022，19，2： | 0 |
| 17 | Cloning,expression and function of the extracellular fragment of human TRAIL gene显示文摘To obtain the recombinant soluble protein of the extracellular fragment of human TRAIL gene and to identify its function preliminarily,this gene fragment was amplified from peripheral blood mononu- clear cells(PBMC)by RT-PCR and cloned into vector pGEM-T-Easy for sequence analysis.The expres- sion vector pET-30a/TRAIL was then constructed by DNA recombination method with a His-tag gene at the front of the TRAIL fragment,and the recombinant protein was expressed in E.coli BL21(DE3). Meanwhile,the expressed target protein was purified with Ni-NTA chromatography column and identified by SDS-PAGE and Western blotting.The proliferation inhibition activity of TRAIL-His was detected by MTT assay.PI staining and Wright-Giemsa staining were used to detect the presence of the TRAIL-in- duced cell apoptosis.It was demonstrated that the target protein expressed in E.coli BL21 showed the same relative molecular mass as that the protein expected and could be recognized by both the anti-TRAIL polyclonal antibody and anti-His monoclonal antibody.In addition,this protein could also inhibit prolif- eration of human lymphoma cell line Jurkat cells or induce apoptosis of this cell line.It is apparent that a recombinant soluble TRAIL protein with biological activity is obtained and this prospective study can lay solid foundation for further research on the biological activity and application in the anti-tumor therapy. | Yu GANG WANG KUN PENG ZHAO Ju GAO CHEN YAN LI YUANG FANG MA BEI FEN SHEN | 2007 | Journal of Microbiology and Immunology2007，5，2： | 0 |
| 18 | 3D spiral channels combined with flexible micro-sieve for high-throughput rare tumor cell enrichment and assay from clinical pleural effusion samples显示文摘The sieving and enrichment of rare tumor cells from large-volume pleural effusion(PE)samples is a promising technique for cell-based lung tumor diagnosis and drug tests,which features high throughput and recovery,purification,as well as viability rates of rare target cells as the prerequisites for high sensitivity,specificity,and accuracy of tumor cell analysis.In this paper,we propose a three-dimensional(3 D)sieving method for rare tumor cell enrichment,which effectively eliminates the'dead zones'in traditional two-dimensional(2 D)cell filters with a dimension-raising strategy to satisfy the requirements mentioned above.The prototype device was combined with a funnel-shaped holder,a flexible micropore membrane in the middle,and a3 D spiral fluid channel covered on the membrane as a three-layer ice-creaming cone composite structure.Driven by gravity alone,the device performed as follows:(1)20-fold throughput compared with the 2 D commercial planee hich was up to 20 mL/min for a threefold dilution of whole blood sample;(2)high recovery rates of 84.5%±21%,86%±25%,83%±14%for 100,1000,and 10000 cells/mL,respectively,in 30 mL phosphate buffer saline(PBS)sample,and a 100%positive detection rate in the case of≤5 A549 cells in 1 mL PBS;(3)a typical purification rate of 85.5%±9.1%;and(4)a viability rate of>93%.In the demonstration application,this device effectively enriched rare target cells from large volumes(>25 mL)of clinical pleural effusions.The following results indicated that tumor cells were easy-to-discover in the enriched PE samples,and the proliferation capability of purified cells was(>4.6 times)significantly stronger than that of unprocessed cells in the subsequent 6-day culture.The above evaluation indicates that the proposed easily reproducible method for the effective execution of rare cell enrichments and assays is expected to become a practical technique for clinical cell-based tumor diagnosis. | Jie Cheng Lina Zhang Yiran Zhang Yifei Ye Wenjie Zhao Lingqian Zhang Yuang Li Yang Liu Wenchang Zhang Hongyan Guo Mingxiao Li Yang Zhao Chengjun Huang | 2022 | Bio-Design and Manufacturing2022，5，2： | 0 |
| 19 | Enhancing photodynamic inactivation via tunning spatial constraint on photosensitizer显示文摘The critical factor of spatial constraint,provided by the external confinement(e.g.,matrix),is often overlooked during photodynamic inactivation,despite playing a crucial role in determining the molecular photophysical process and subsequent antipathogen performance.Here,as a proof-of-concept model,we employed two types of polymers with varying interaction energies with dopants to investigate the intrinsic relationship between spatial constraint and the essential excited-state behaviors of doped photosensitizer(4-(2-(5-(4-(diphenylamino)phenyl)thiophen-2-yl)ethyl)-1-methylquinolin-1-ium iodine,TPP).Through experimental investigation and theoretical calculations,we found that TPP tends to remain in the excited state for a shorter dwell time under weaker spatial constraints due to less restricted molecular motion in polyurethane(PU) nanofibers.Consequently,the singlet oxygen(^(1)O_(2)) generated from doped-TPP shows a 9.23-fold enhancement in PU than in the polyvinylchloride(PVC) matrix.Under light irradiation,the PU@TPP nanofiber can efficiently eliminate the coronavirus MHV-A59(≥99.9997%) at a 220,000-fold higher concentration than the infected space.Its antibacterial efficacy has also been demonstrated,with a killing rate of ≥99%. | Chong Li Minghao Han Ji Gao Shuxian Wang Song-Bo Lu Yaxi Li Zhao Liu Chen Zhang Zhaojin Wang Fangliang Wang Yuang Sun Chun Loong Ho Kai Wang Yun Shen Kai Li | 2024 | Science China Chemistry2024，67，2： | 0 |
| 20 | piRT-IFC:Physics-informed real-time impedance flow cytometry for the characterization of cellular intrinsic electrical properties显示文摘Real-time transformation was important for the practical implementation of impedance flow cytometry.The major obstacle was the time-consuming step of translating raw data to cellular intrinsic electrical properties(e.g.,specific membrane capacitance C_(sm) and cytoplasm conductivityσ_(cyto)).Although optimization strategies such as neural network-aided strategies were recently reported to provide an impressive boost to the translation process,simultaneously achieving high speed,accuracy,and generalization capability is still challenging.To this end,we proposed a fast parallel physical fitting solver that could characterize single cells’C_(sm)andσ_(cyto)within 0.62 ms/cell without any data preacquisition or pretraining requirements.We achieved the 27000-fold acceleration without loss of accuracy compared with the traditional solver.Based on the solver,we implemented physics-informed real-time impedance flow cytometry(piRT-IFC),which was able to characterize up to 100,902 cells’C_(sm) andσ_(cyto)within 50 min in a real-time manner.Compared to the fully connected neural network(FCNN)predictor,the proposed real-time solver showed comparable processing speed but higher accuracy.Furthermore,we used a neutrophil degranulation cell model to represent tasks to test unfamiliar samples without data for pretraining.After being treated with cytochalasin B and N-Formyl-Met-Leu-Phe,HL-60 cells underwent dynamic degranulation processes,and we characterized cell’s C_(sm)andσ_(cyto)using piRT-IFC.Compared to the results from our solver,accuracy loss was observed in the results predicted by the FCNN,revealing the advantages of high speed,accuracy,and generalizability of the proposed piRT-IFC. | Xiaofeng Luan Pengbin Liu Di Huang Haiping Zhao Yuang Li Sheng Sun Wenchang Zhang Lingqian Zhang Mingxiao Li Tian Zhi Yang Zhao Chengjun Huang | 2023 | Microsystems & Nanoengineering2023，9，3： | 0 |
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