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| 1 | Arabidopsis Blue Light Receptor Phototropin 1 Undergoes Blue Light-Induced Activation in Membrane Microdomains显示文摘Phototropin (辐透) 调停了发信号由蓝光(BL ) 开始了在在植物在血浆膜(下午) 优化光合的轻俘获起一个关键作用。然而,响应 BL 在 PM 位于辐透活动的规定下面的机制仍然保持大部分不清楚。在这研究,由单个粒子的追踪和 phot1-GFP 蛋白质的逐步的 photobleaching 分析,我们在黑暗 phot1 表明了那 ? 蛋白质留在一个不活跃的状态并且主要作为单体存在。Dimerization 和 phot1-GFP 的散开率响应 BL 以一种剂量依赖者方式增加了。相反, BL 没影响 kinase 不活跃的 phot1 D806N -GFP?but 确实提高了它的 dimerization,建议那 phot1 dimerization 独立于 phosphorylation。F ? rster 回声精力转移荧光一生成像显微镜学 ? 分析表明在 phot1-GFP 和充满甾醇的类脂化合物环境的一个标记之间的相互作用, AtRem1.3-mCherry,与 BL 处理的增加的时间被提高。然而,这个 BL 依赖的相互作用不在植物共同表示是明显的 phot1 D806N -GFP 和 AtRem1.3-mCherry,显示那 BL 便于功能的 phot1-GFP 的 translocation 进标记 AtRem1.3 的 microdomains 激活调停辐透的发信号。相反地,甾醇弄空稀释了 phot1-GFP 动力学, dimerization,和 phosphorylation。一起拿,这些结果显示膜 microdomains 充当在 PM 组织为激活的 phot1 的合适的功能必要的平台。 | Yiqun Xue Jingjing Xing Yinglang Wan Xueqin Lv Lusheng Fan Yongdeng Zhang Kai Song Li Wang Xiaohua Wang Xin Deng Frantisek Baluska John M. Christie Jinxing Lin | 2018 | Molecular Plant2018，11，6： | 4 |
| 2 | Spatial distribution of IL4 controls iNKT cell-DC crosstalk in tumors显示文摘The spatiotemporal distribution of cytokines orchestrates immune responses in vivo,yet the underlying mechanisms remain to be explored.We showed here that the spatial distribution of interleukin-4(IL4)in invariant natural killer T(iNKT)cells regulated crosstalk between iNKT cells and dendritic cells(DCs)and controlled iNKT cell-mediated T-helper type 1(Th1)responses.The persistent polarization of IL4 induced by strong lipid antigens,that is,α-galactosylceramide(αGC),caused IL4 accumulation at the immunological synapse(IS),which promoted the activation of the IL4R-STAT6(signal transducer and activator of transcription 6)pathway and production of IL12 in DCs,which enhanced interferon-γ(IFNγ)production in iNKT cells.Conversely,the nonpolarized secretion of IL4 induced by Th2 lipid antigens with a short or unsaturated chain was incapable of enhancing this iNKT cell-DC crosstalk and thus shifted the immune response to a Th2-type response.The nonpolarized secretion of IL4 in response to Th2 lipid antigens was caused by the degradation of Cdc42 in iNKT cells.Moreover,reduced Cdc42 expression was observed in tumorinfiltrating iNKT cells,which impaired IL4 polarization and disturbed iNKT cell-DC crosstalk in tumors. | Lu Wang Zhilan Liu Lili Wang Qielan Wu Xiang Li Di Xie Huimin Zhang Yongdeng Zhang Lusheng Gu Yanhong Xue Ting Yue Gang Liu Wei Ji Haiming Wei Tao Xu Li Bai | 2020 | Cellular & Molecular Immunology2020，17，5： | 4 |
| 3 | Ultrafast,accurate,and robust localization of anisotropic dipoles显示文摘The resolution of single molecule localization imaging techniques largely depends on the precision of localization algorithms.However,the commonly used Gaussian function is not appropriate for anisotropic dipoles because it is not the true point spread function.We derived the theoretical point spread function of tilted dipoles with restricted mobility and developed an algorithm based on an artifi cial neural network for estimating the localization,orientation and mobility of individual dipoles.Compared with fi tting-based methods,our algorithm demonstrated ultrafast speed and higher accuracy,reduced sensitivity to defocusing,strong robustness and adaptability,making it an optimal choice for both two-dimensional and threedimensional super-resolution imaging analysis. | Yongdeng Zhang Lusheng Gu Hao Chang Wei Ji Yan Chen Mingshu Zhang Lu Yang Bei Liu Liangyi Chen Tao Xu | 2013 | Protein & Cell2013，4，8： | 2 |
| 4 | RAB-27 and its effector RBF-1 regulate the tethering and docking steps of DCV exocytosis in C.elegans显示文摘The molecular mechanisms by which dense core vesicles(DCVs) translocate,tether,dock and prime are poorly understood.In this study,Caenorhabditis elegans was used as a model organism to study the function of Rab proteins and their effectors in DCV exocytosis.RAB-27/AEX-6,but not RAB-3,was found to be required for peptide release from neurons.By analyzing the movement of DCVs approaching the plasma membrane using total internal reflection fluorescence microscopy,we demonstrated that RAB-27/AEX-6 is involved in the tethering of DCVs and that its effector rabphilin/RBF-1 is required for the initial tethering and subsequent stabilization by docking. | FENG Wan Juan LIANG Tao YU JunWei ZHOU Wei ZHANG YongDeng WU ZhengXing XU Tao | 2012 | Science China(Life Sciences)2012，55，3： | 1 |
| 5 | C13C4.5/Spinster, an evolutionarily conserved protein that regulates fertility in C. elegans through a lysosome-mediated lipid metabolism process显示文摘Lipid droplets, which are conserved across almost all species, are cytoplasmic organelles used to store neutral lipids. Identification of lipid droplet regulators will be conducive to resolving obesity and other fat-associated diseases. In this paper, we selected 11 candidates that might be associated with lipid metabolism in Caenorhabditis elegans . Using a BODIPY 493/503-based flow cytometry screen, 6 negative and 3 positive regulators of fat content were identified. We selected one negative regulator of lipid content, C13C4.5 , for future study. C13C4.5 was mainly expressed in the worm intestine. We found that this gene was important for maintaining the metabolism of lipid droplets. Biochemical results revealed that 50% of triacylglycerol (TAG) was lost in C13C4.5 knockout worms. Stimulated Raman scattering (SRS) signals in C13C4.5 mutants showed only 49.6% of the fat content in the proximal intestinal region and 86.3% in the distal intestinal region compared with wild type animals. The mean values of lipid droplet size and intensity in C13C4.5 knockout animals were found to be significantly decreased compared with those in wild type worms. The LMP-1-labeled membrane structures in worm intestines were also enlarged in C13C4.5 mutant animals. Finally, fertility defects were found in C13C4.5 ( ok2087 ) mutants. Taken together, these results indicate that C13C4.5 may regulate the fertility of C. elegans by changing the size and fat content of lipid droplets by interfering with lysosomal morphology and function. | Mei Han Hao Chang Peng Zhang Tao Chen Yanhua Zhao Yongdeng Zhang Pingsheng Liu Tao Xu Pingyong Xu | 2013 | Protein & Cell2013，4，5： | 1 |
| 6 | Super-resolution microscopy of live cells using single molecule localization显示文摘The resolution of conventional light microscopy is insufficient for subcelluar studies.The invention of various super-resolution imaging techniques breaks the diffraction barrier and pushes the resolution limit towards the nanometer scale.Here,we focus on a category of super-resolution microscopy that relies on the stochastic activation and precise localization of single molecules.A diversity of fluorescent probes with different characteristics has been developed to achieve super-resolution imaging.In addition,with the implementation of robust localization algorithms,this family of approaches has been expanded to multi-color,three-dimensional and live cell imaging,which provides a promising prospect in biological research. | ZHANG YongDeng CHANG Hao GU LuSheng ZHAO YanHua XU Tao JI Wei | 2013 | Chinese Science Bulletin2013，58，36： | 0 |
| 7 | Adaptive optics in super-resolution microscopy显示文摘Fluorescence microscopy has become a routine tool in biology for interrogating life activities with minimal perturbation.While the resolution of fluorescence microscopy is in theory governed only by the diffraction of light,the resolution obtainable in practice is also constrained by the presence of optical aberrations.The past two decades have witnessed the advent of super-resolution microscopy that overcomes the diffraction barrier,enabling numerous biological investigations at the nanoscale.Adaptive optics,a technique borrowed from astronomical imaging,has been applied to correct for optical aberrations in essentially every microscopy modality,especially in super-resolution microscopy in the last decade,to restore optimal image quality and resolution.In this review,we briefly introduce the fundamental concepts of adaptive optics and the operating principles of the major super-resolution imaging techniques.We highlight some recent implementations and advances in adaptive optics for active and dynamic aberration correction in super-resolution microscopy. | Jingyu Wang Yongdeng Zhang | 2021 | Biophysics Reports2021，7，4： | 0 |
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