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| 1 | Spatial distribution of IL4 controls iNKT cell-DC crosstalk in tumors显示文摘The spatiotemporal distribution of cytokines orchestrates immune responses in vivo,yet the underlying mechanisms remain to be explored.We showed here that the spatial distribution of interleukin-4(IL4)in invariant natural killer T(iNKT)cells regulated crosstalk between iNKT cells and dendritic cells(DCs)and controlled iNKT cell-mediated T-helper type 1(Th1)responses.The persistent polarization of IL4 induced by strong lipid antigens,that is,α-galactosylceramide(αGC),caused IL4 accumulation at the immunological synapse(IS),which promoted the activation of the IL4R-STAT6(signal transducer and activator of transcription 6)pathway and production of IL12 in DCs,which enhanced interferon-γ(IFNγ)production in iNKT cells.Conversely,the nonpolarized secretion of IL4 induced by Th2 lipid antigens with a short or unsaturated chain was incapable of enhancing this iNKT cell-DC crosstalk and thus shifted the immune response to a Th2-type response.The nonpolarized secretion of IL4 in response to Th2 lipid antigens was caused by the degradation of Cdc42 in iNKT cells.Moreover,reduced Cdc42 expression was observed in tumorinfiltrating iNKT cells,which impaired IL4 polarization and disturbed iNKT cell-DC crosstalk in tumors. | Lu Wang Zhilan Liu Lili Wang Qielan Wu Xiang Li Di Xie Huimin Zhang Yongdeng Zhang Lusheng Gu Yanhong Xue Ting Yue Gang Liu Wei Ji Haiming Wei Tao Xu Li Bai | 2020 | Cellular & Molecular Immunology2020，17，5： | 4 |
| 2 | Ultra-stable super-resolution fluorescence cryo-microscopy for correlative light and electron cryo-microscopy显示文摘Remarkable progress in correlative light and electron cryo-microscopy(cryo-CLEM) has been made in the past decade. A crucial component for cryo-CLEM is a dedicated cryo-fluorescence microscope(cryo-FM). Here, we describe an ultra-stable superresolution cryo-FM that exhibits excellent thermal and mechanical stability. The temperature fluctuations in 10 h are less than0.06 K, and the mechanical drift over 5 h is less than 200 nm in three dimensions. We have demonstrated the super-resolution imaging capability of this system(average single molecule localization accuracy of ~13.0 nm). The results suggest that our system is particularly suitable for long-term observations, such as single molecule localization microscopy(SMLM) and cryogenic super-resolution correlative light and electron microscopy(csCLEM). | Xiaojun Xu Yanhong Xue Buyun Tian Fengping Feng Lusheng Gu Weixing Li Wei Ji Tao Xu | 2018 | Science China(Life Sciences)2018，61，11： | 3 |
| 3 | Ultrafast,accurate,and robust localization of anisotropic dipoles显示文摘The resolution of single molecule localization imaging techniques largely depends on the precision of localization algorithms.However,the commonly used Gaussian function is not appropriate for anisotropic dipoles because it is not the true point spread function.We derived the theoretical point spread function of tilted dipoles with restricted mobility and developed an algorithm based on an artifi cial neural network for estimating the localization,orientation and mobility of individual dipoles.Compared with fi tting-based methods,our algorithm demonstrated ultrafast speed and higher accuracy,reduced sensitivity to defocusing,strong robustness and adaptability,making it an optimal choice for both two-dimensional and threedimensional super-resolution imaging analysis. | Yongdeng Zhang Lusheng Gu Hao Chang Wei Ji Yan Chen Mingshu Zhang Lu Yang Bei Liu Liangyi Chen Tao Xu | 2013 | Protein & Cell2013，4，8： | 2 |
| 4 | Targeted disruption of RablO causes early embryonic lethality显示文摘 | Pingping Lv Yi Sheng Zhenao Zhao Wei Zhao Lusheng Gu Tao Xu Eli Song | 2015 | Protein & Cell2015，6，6： | 1 |
| 5 | Study on the Supporting Policies of SMEs: Empirical Research in Shaanxi Province显示文摘 | Liandang Dong Ying Gu Lusheng Fang | 2006 | China-USA Business Review2006，5，1： | 0 |
| 6 | Tetra-color superresolution microscopy based on excitation spectral demixing显示文摘Multicolor imaging allows protein colocalizations and organelle interactions to be studied in biological research,which is especially important for single-molecule localization microscopy(SMLM).Here,we propose a multicolor method called excitation-resolved stochastic optical reconstruction microscopy(ExR-STORM).The method,which is based on the excitation spectrum of fluorescent dyes,successfully separated four spectrally very close far-red organic fluorophores utilizing three excitation lasers with cross-talk of less than 3%.Dyes that are only 5 nm apart in the emission spectrum were resolved,resulting in negligible chromatic aberrations.This method was extended to three-dimensional(3D)imaging by combining the astigmatic method,providing a powerful tool for resolving 3D morphologies at the nanoscale. | Wanyan Wu Shihang Luo Chunyan Fan Tianjie Yang Shuwen Zhang Wenxiang Meng Tao Xu Wei Ji Lusheng Gu | 2023 | Light(Science & Applications)2023，12，1： | 0 |
| 7 | Super-resolution microscopy of live cells using single molecule localization显示文摘The resolution of conventional light microscopy is insufficient for subcelluar studies.The invention of various super-resolution imaging techniques breaks the diffraction barrier and pushes the resolution limit towards the nanometer scale.Here,we focus on a category of super-resolution microscopy that relies on the stochastic activation and precise localization of single molecules.A diversity of fluorescent probes with different characteristics has been developed to achieve super-resolution imaging.In addition,with the implementation of robust localization algorithms,this family of approaches has been expanded to multi-color,three-dimensional and live cell imaging,which provides a promising prospect in biological research. | ZHANG YongDeng CHANG Hao GU LuSheng ZHAO YanHua XU Tao JI Wei | 2013 | Chinese Science Bulletin2013，58，36： | 0 |
| 8 | Recent progress on single-molecule localization microscopy显示文摘Super-resolution imaging based on single-molecule localization has been developed for more than a decade.These techniques can break through diffraction limit of fluorescent microscopy and initially improve the resolution by an order of magnitude to~20 nm,by introducing photoactivatable/photoswitching probes and centroid fitting method.As the demand of biological research,the localization precision of single-molecules was further improved by several state-of-the-art methods in the past several years.This review focuses on the latest developed techniques which have greatly improved the performance of single-molecule localization microscopy,from measurement principle to hardware design.These methods are essential for the study of nanostructures and biomacromolecule dynamics inside of cells. | Lusheng Gu Wei Ji | 2021 | Biophysics Reports2021，7，5： | 0 |
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