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| 1 | Integrating full-length transcriptomics and metabolomics reveals the regulatory mechanisms underlying yellow pigmentation in tree peony(Paeonia suffruticosa Andr.)flowers显示文摘Tree peony(Paeonia suffruticosa Andr.)is a popular ornamental plant in China due to its showy and colorful flowers.However,yellow-colored flowers are rare in both wild species and domesticated cultivars.The molecular mechanisms underlying yellow pigmentation remain poorly understood.Here,petal tissues of two tree peony cultivars,“High Noon”(yellow flowers)and“Roufurong”(purple–red flowers),were sampled at five developmental stages(S1–S5)from early flower buds to full blooms.Five petal color indices(brightness,redness,yellowness,chroma,and hue angle)and the contents of ten different flavonoids were determined.Compared to“Roufurong,”which accumulated abundant anthocyanins at S3–S5,the yellow-colored“High Noon”displayed relatively higher contents of tetrahydroxychalcone(THC),flavones,and flavonols but no anthocyanin production.The contents of THC,flavones,and flavonols in“High Noon”peaked at S3 and dropped gradually as the flower bloomed,consistent with the color index patterns.Furthermore,RNA-seq analyses at S3 showed that structural genes such as PsC4Hs,PsDFRs,and PsUFGTs in the flavonoid biosynthesis pathway were downregulated in“High Noon,”whereas most PsFLSs,PsF3Hs,and PsF3’Hs were upregulated.Five transcription factor(TF)genes related to flavonoid biosynthesis were also upregulated in“High Noon.”One of these TFs,PsMYB111,was overexpressed in tobacco,which led to increased flavonols but decreased anthocyanins.Dual-luciferase assays further confirmed that PsMYB111 upregulated PsFLS.These results improve our understanding of yellow pigmentation in tree peony and provide a guide for future molecular-assisted breeding experiments in tree peony with novel flower colors. | Xiaoning Luo Daoyang Sun Shu Wang Sha Luo Yaqi Fu Lixin Niu Qianqian Shi Yanlong Zhang | 2021 | Horticulture Research2021，8，1： | 5 |
| 2 | N-myc downstream-regulated gene 2 promotes proliferation of HO-8910 ovarian cancer cells显示文摘Objective To investigate N-myc downstream-regulated gene 2(NDRG2) expression in ovarian cancer cells and its potential usefulness as a diagnostic marker and/or target for therapeutic intervention.Methods Human NDRG2 L/S gene was obtained by revers-transcription polymerase chain reaction(RT-PCR). Sequence analysis confirmed the identity of NDRG2 L/S gene, which was then inserted into a eukaryotic vector p LNCX2, which was in turn transfected into NDRG2 gene-negative HO-8910 cells. Flow cytometry(FCM) and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide(MTT) assay were conducted to determine the proliferation rate of HO-8910 cells. Cisplatin resistance of HO-8910 cells transfected with p LNCX2-NDRG2 L/S was evaluated by FCM. Tumors were generated in female nude mice by subcutaneous injection of HO-8910 cells.Results NDRG2 gene was isolated and its expression vector was successfully constructed. NDRG2 expression positively correlated with the proliferation of HO-8910 cells. NDRG2 L/S promoted tumorigenicity in HO-8910 cells.Conclusion The present study identified a novel function of NDRG2 L/S gene and demonstrated its involvement in the promotion of ovarian cancer cell proliferation and enhancement of cisplatin resistance in HO-8910 cells. Future studies are warranted to determine the relationship between NDRG2 upregulation and ovarian cancer progression. | Fenhong Kang Yaping Luo Yanlong Wang | 2018 | Oncology and Translational Medicine2018，4，4： | 2 |
| 3 | Three‐Dimensional Co3O4@MnO2 Hierarchical Nanoneedle Arrays: Morphology Control and Electrochemical Energy Storage显示文摘 | Dezhi Kong Jingshan Luo Yanlong Wang Weina Ren Ting Yu Yongsong Luo Yaping Yang Chuanwei Cheng | 2014 | Adv. Funct. Mater2014，，24： | 2 |
| 4 | Hydrogen bond-assisted 1,2-cis O-glycosylation under mild hydrogenolytic conditions显示文摘A hydrogen bond-assisted α-selective glycosylation reaction by using 4,6-dibenzyloxy-1,3,5-triazin-2-yl(DBT) β-glycosyl donors was developed for the efficient construction of 1,2-cis-α-glycosidic bond in natural products. This method was applied successfully to the direct synthesis of complex oligosaccharidederived glycolipids with simple protecting chemistry. Mechanistic studies using the NMR spectroscopy and DFT calculation provide a proof of concept for hydrogen bond-assisted glycosylation reaction towardsα-specific construction of O-glycosidic linkage. | Gefei Li Yanlong Luo Juan Mo Masato Noguchi Jie Jing Zhenyang Luo Shin-ichiro Shoda Xin-Shan Ye | 2023 | Chinese Chemical Letters2023，34，4： | 1 |
| 5 | African swine fever virus MGF505-3R inhibits cGAS-STING-mediated IFN-βpathway activation by degrading TBK1显示文摘African swine fever virus(ASFV)is an important pathogen causing acute infectious disease in domestic pigs and wild boars that seriously endangers the global swine industry.As ASFV is structurally complex and encodes a large number of functional proteins,no effective vaccine has been developed to date.Thus,dissecting the mechanisms of immune escape induced by ASFV proteins is crucial.A previous study showed that the ASFV-encoded protein is an important factor in host immunity.In this study,we identified a negative regulator,MGF505-3R,that significantly downregulated cGAS/STING-and poly(dG:dC)-mediated IFN-βand interferon stimulation response element(ISRE)reporter activity and suppressed IFNB1 and IFIT2 mRNA levels.In addition,TBK1,IRF3 and IκBαphosphorylation levels were also inhibited.Mechanistically,MGF505-3R interacted with cGAS/TBK1/IRF3 and targeted TBK1 for degradation,thereby disrupting the cGAS-STING-mediated IFN-βsignaling pathway,which appears to be highly correlated with autophagy.Knockdown MGF505-3R expression enhanced IFN-βand IL-1βproduction.Taken together,our study revealed a negative regulatory mechanism involving the MGF505-3R-cGAS-STING axis and provided insights into an evasion strategy employed by ASFV that involves autophagy and innate signaling pathways. | Mingyang Cheng Jiawei Luo Yuetong Duan Yu Yang Chunwei Shi Yu Sun Yiyuan Lu Junhong Wang Xiaoxu Li Jianzhong Wang Nan Wang Wentao Yang Yanlong Jiang Guilian Yang Yan Zeng Chunfeng Wang Xin Cao | 2022 | Animal Diseases2022，2，3： | 1 |
| 6 | Pyrrole synthesis in ionic liquids by Paal-Knorr condensation under mild conditions显示文摘 | WANG BO GU YANLONG LUO CHENG | | 0，，45： | 1 |
| 7 | Bioinspired surface functionalization of biodegradable mesoporous silica nanoparticles for enhanced lubrication and drug release显示文摘Osteoarthritis is associated with the significantly increased friction of the joint,which results in progressive and irreversible damage to the articular cartilage.A synergistic therapy integrating lubrication enhancement and drug delivery is recently proposed for the treatment of early-stage osteoarthritis.In the present study,bioinspired by the self-adhesion performance of mussels and super-lubrication property of articular cartilages,a biomimetic self-adhesive dopamine methacrylamide-poly(2-methacryloyloxyethyl phosphorylcholine)(DMA-MPC)copolymer was designed and synthesized via free radical polymerization.The copolymer was successfully modified onto the surface of biodegradable mesoporous silica nanoparticles(bMSNs)by the dip-coating method to prepare the dual-functional nanoparticles(bMSNs@DMA-MPC),which were evaluated using a series of surface characterizations including the transmission electron microscope(TEM),Fourier transform infrared(FTIR)spectrum,thermogravimetric analysis(TGA),X-ray photoelectron spectroscopy(XPS),etc.The tribological test and in vitro drug release test demonstrated that the developed nanoparticles were endowed with improved lubrication performance and achieved the sustained release of an anti-inflammatory drug,i.e.,diclofenac sodium(DS).In addition,the in vitro biodegradation test showed that the nanoparticles were almost completely biodegraded within 10 d.Furthermore,the dual-functional nanoparticles were biocompatible and effectively reduced the expression levels of two inflammation factors such as interleukin-1β(IL-1β)and interleukin-6(IL-6).In summary,the surface functionalized nanoparticles with improved lubrication and local drug release can be applied as a potential intra-articularly injected biolubricant for synergistic treatment of early-stage osteoarthritis. | Xiaowei MAO Kexin CHEN Yanlong ZHAO Chunrong Xiong Jing LUO Yuguang WANG Bo WANG Hongyu ZHANG | 2023 | Friction2023，11，7： | 0 |
| 8 | Vibro-impact dynamics near a strong resonance point显示文摘有影响的二个典型振动系统被考虑其一是 two-degree-of-freedom 影响非强迫的僵硬身体的振动系统,另外的影响一个僵硬振幅站。如此的模型与重复影响在机械系统的动力学的研究起一个重要作用。在 vibro 影响系统的固定的点的二参数的分叉,把强壮的回声与 1:4 联系了,被为地图使用歧管的中心和正常表格方法分析。单个影响的周期的运动和 vibro 影响系统的 Poincar é地图是导出的经分解。稳定性和一个单个影响的周期的运动的本地分叉被使用 Poincar é地图分析。一个中心歧管定理技术被使用把 Poincar é地图归结为二维的,和正常形式地图因为 1:4 回声被获得。在为 1:4 回声的分叉点附近,二个 vibro 影响系统的本地行为被学习。在那里为 1:4 在分叉附近削尖强壮的回声存在时期一单个影响的运动和正切(褶层) 的 Neimark Sacker 分叉时期 4 四影响的运动的分叉,等等。从模拟的结果显示出 vibro 影响系统的动力学的一些有趣的特征:也就是,“圆由与僵绳的稳定、不稳定的 separatrices 一致形成了的异种诊所”, T 在, T 在和 T 外面类型正切(褶层)分叉,与时期四联系的伪周期的影响轨道四影响并且时期八八影响的运动,等等。到混乱的时期 4 四影响的运动的不同线路被数字模拟, vibro 影响系统在展出很复杂的伪周期的影响运动获得。 | Guanwei Luo Yanlong Zhang Jianhua Xie Jiangang Zhang | 2007 | Acta Mechanica Sinica2007，23，3： | 0 |
| 9 | An Easy‑to‑Prepare Flexible Dual‑Mode Fiber Membrane for Daytime Outdoor Thermal Management显示文摘Excellent outdoor thermal management is vital for public health and safety.However,the ever-changing and uncontrol-lable outdoor environmental conditions,such as irrepressible sunlight and drastic temperature fluctuations,bring majestic challenges to outdoor thermal management.Here,we report a significant advancement toward designing and fabricating a novel fiber membrane with a dual-function of radiative cooling and solar heating for an efficient daytime outdoor thermal management.Unlike the reported dual-mode thermal management materials,which are usually fabricated by compounding organic polymers and metal/inorganic non-metal materials,our fiber-based membrane is composed of only two polymers,i.e.,poly(vinylidene fluoride-co-hexafluoropropene)(PVDF-HFP)and polypyrrole(PPy).The resulting membrane presents outstanding outdoor thermoregulation capacity,with a practically attainable sun-ambient cooling temperature of~4.5℃,super-ambient heating temperature of~35.8℃,in an outdoor environment,under solar intensity(Isolar)of~850 W m^(-2).Fabrication process is simple and cost-effective,which offers the possibility of preparing large-scale products.Owing to the scalable and simple fabrication process,and the exceptional outdoor thermoregulation ability,this dual-mode fiber membrane has great potential to maintain a comfortable outdoor environment for human activities and industrial operations. | Bo Xiang Rong Zhang Xujia Zeng Yanlong Luo Zhenyang Luo | 2022 | Advanced Fiber Materials2022，4，5： | 0 |
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