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| 1 | Adenosine monophosphate-activated protein kinase activation enhances embryonic neural stem cell apoptosis in a mouse model of amyotrophic lateral sclerosis显示文摘Alterations in embryonic neural stem cells play crucial roles in the pathogenesis of amyotrophic lateral sclerosis. We hypothesized that embryonic neural stem cells from SOD1G93 A individuals might be more susceptible to oxidative injury, resulting in a propensity for neurodegeneration at later stages. In this study, embryonic neural stem cells obtained from human superoxide dismutase 1 mutant(SOD1G93A) and wild-type(SOD1WT) mouse models were exposed to H2O2. We assayed cell viability with mitochondrial succinic dehydrogenase colorimetric reagent, and measured cell apoptosis by flow cytometry. Moreover, we evaluated the expression of the adenosine monophosphate-activated protein kinase(AMPK) α-subunit, paired box 3(Pax3) protein, and p53 in western blot analyses. Compared with SOD1 WT cells, SOD1G93 A embryonic neural stem cells were more likely to undergo H2O2-induced apoptosis. Phosphorylation of AMPKα in SOD1G93 A cells was higher than that in SOD1 WT cells. Pax3 expression was inversely correlated with the phosphorylation levels of AMPKα. p53 protein levels were also correlated with AMPKαphosphorylation levels. Compound C, an inhibitor of AMPKα, attenuated the effects of H2O2. These results suggest that embryonic neural stem cells from SOD1G93 A mice are more susceptible to apoptosis in the presence of oxidative stress compared with those from wild-type controls, and the effects are mainly mediated by Pax3 and p53 in the AMPKα pathway. | Yanling Sui Zichun Zhao Rong Liu Bin Cai Dongsheng Fan | 2014 | Neural Regeneration Research2014，9，19： | 3 |
| 2 | The mechanism and characterization of dye-free coloration on tussah silk based on tryptophan 显示文摘 | SUI Yanling WEI Chunyan LIU Wenjing | 2011 | Advanced Materials Research2011，，： | 1 |
| 3 | The mechanism and characterization of dye-free coloration on tussah silk based on tryptophan 显示文摘 | SUI Yanling WEI Chunyan LIU Wenjing | 2011 | Advanced Materials Research2011，，175176： | 1 |
| 4 | The mechanism and characterization of dye-free coloration on tussah silk based on tryptophan 显示文摘 | SUI Yanling WEI Chunyan LIU Wenjing | 2011 | Advanced Materials Research2011，，175： | 1 |
| 5 | A drone‑and feld‑based investigation of the land degradation and soil erosion at an opencast coal mine dump after 5 years’evolution of natural processes显示文摘Opencast coal mining has a large impact on the land surface,both at the mining pits themselves and at waste sites.After artifcial management is stopped,a reclaimed opencast coal mine dump is afected by wind and water erosion from natural processes,resulting in land degradation and even safety incidents.In this paper,the soil erosion and land degradation after 5 years of such natural processes,at the Xilinhot opencast coal mine dump in Inner Mongolia,were investigated.A multisource data acquisition method was applied:the vegetation fraction coverage(VFC)was extracted from GF-1 satellite imagery,high-precision terrain characteristics and the location and degree of soil erosion were obtained using a drone,and the physical properties of the topsoil were obtained by feld sampling.On this basis,the degree and spatial distribution of erosion cracks were identifed,and the causes of soil erosion and land degradation were analyzed using the geographical detector.The results show that(1)multi-source data acquisition method can provide efective basic data for the quantitative evaluation of the ecological environment at dumps,and(2)slope aspect and VFC are the main factors afecting the degree of degradation and soil erosion.Based on above analysis,several countermeasures are proposed to mitigate land degradation:(1)The windward slope be designed to imitate the natural landform.(2)Reasonable engineering measures should be applied at the slope to restrain soil erosion.(3)The Pioneer plants should be widely planted on the platform at the early stage of reclamation. | Wu Xiao He Ren Tao Sui Heyu Zhang Yanling Zhao Zhenqi Hu | 2022 | International Journal of Coal Science & Technology2022，9，3： | 0 |
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