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| 1 | Fast-Suppressor Screening for New Components in Protein Trafficking,Organelle Biogenesis and Silencing Pathway in Arabidopsis thaliana Using DEX-Inducible FREE1-RNAi Plants显示文摘Membrane trafficking is essential for plant growth and responses to external signals.The plant unique FYVE domain-containing protein FREE1 is a component of the ESCRT complex(endosomal sorting complex required for transport).FREE1 plays multiple roles in regulating protein trafficking and organelle biogenesis including the formation of intraluminal vesicles of multivesicular body(MVB),vacuolar protein transport and vacuole biogenesis,and autophagic degradation.FREE1 knockout plants show defective MVB formation,abnormal vacuolar transport,fragmented vacuoles,accumulated autophagosomes,and seedling lethality.To further uncover the underlying mechanisms of FREE1 function in plants,we performed a forward genetic screen for mutants that suppressed the seedling lethal phenotype of FREE1-RNAi transgenic plants.The obtained mutants are termed as suppressors of free1(sof).To date,229 putative sof mutants have been identified.Barely detecting of FREE1 protein with M3plants further identified 84 FREE1-related suppressors.Also145 mutants showing no reduction of FREE1 protein were termed as RNAi-related mutants.Through next-generation sequencing(NGS)of bulked DNA from F2mapping population of two RNAi-related sof mutants,FREE1-RNAi T-DNA inserted on chromosome 1 was identified and the causal mutation of putative sof mutant is being identified similarly.These FREE1-and RNAi-related sof mutants will be useful tools and resources for illustrating the underlying mechanisms of FREE1 function in intracellular trafficking and organelle biogenesis,as well as for uncovering the new components involved in the regulation of silencing pathways in plants. | Qiong Zhao Caiji Gao PoShing Lee Lin Liu Shaofang Li Tangjin Hu Jinbo Shen Shuying Pan Hao Ye Yunru Chen Wenhan Cao Yong Cui Peng Zeng Sheng Yu Yangbin Gao Liang Chen Beixin Mo Xin Liu Shi Xiao Yunde Zhao Silin Zhong Xuemei Chen Liwen Jiang | 2015 | Journal of Genetics and Genomics2015，42，6： | 4 |
| 2 | An Accurate Common-Mode Current Prediction Method for Transformerless Photovoltaic Inverters Based on Graph Theory显示文摘The common-mode current is an important indicator with transformerless photovoltaic inverters.However,up to now,there is not an accurate method to predict common-mode current in the inverter design process,resulting from inappropriate device selection or exceeded the expected common-mode current.In order to solve this problem,this paper proposes an accurate common-mode current prediction method based on graph theory for transformerless photovoltaic inverters.In this paper,the mathematic model of the common-mode current is derived using graph theory analysis method in the full-bridge topology,and it is used to predict common-mode current.The validity and correctness of the proposed prediction method are validated by simulation and experiment.The oscillation frequency and amplitude can be predicted by the proposed common-mode prediction method,whereas the traditional common-mode analysis method cannot.This paper provides a novel way to predict and analyze common-mode current in the transformerless photovoltaic inverters. | Yangbin Zeng Hong Li Bo Zhang Zhichang Yang Trillion Q.Zheng | 2017 | Chinese Journal of Electrical Engineering2017，3，3： | 1 |
| 3 | A New Local Search-based Multiobjective Optimization Algori- thm 显示文摘 | Chen Bili Zeng Wenhua Lin Yangbin | 2015 | IEEE Transactions on Evolutionary Com- putation2015，19，1： | 1 |
| 4 | A new local search- based multiobjective optimization algorithm 显示文摘 | Chen Bili Zeng Wenhua Lin Yangbin | 2015 | IEEE Transactions on Evolutionary Computation2015，19，1： | 1 |
| 5 | Two homologous INDOLE-3-ACETAIVHDE (IAM) HYDROLASE genes are required for the auxin effects of IAM in Arabidopsis显示文摘Indole-3-acetamide(IAM)is the first confirmed auxin biosynthetic intermediate in some plant pathogenic bacteria.Exogenously applied IAM or production of IAM by overexpressing the bacterial iaaM gene in Arabidopsis causes auxin overproduction phenotypes.However,it is still inconclusive whether plants use IAM as a key precursor for auxin biosynthesis.Herein,we reported the isolation IAM HYDROLASE 1(IAMH1)gene in Arabidopsis from a forward genetic screen for IAM-insensitive mutants that display normal auxin sensitivities.IAMH1 has a close homolog named IAMH2 that is located right next to IAMH1on chromosomeⅣin Arabidopsis.We generated iamh1 iamh2 double mutants using our CRISPR/Cas9gene editing technology.We showed that disruption of the IAMH genes rendered Arabidopsis plants resistant to IAM treatments and also suppressed the iaaM overexpression phenotypes,suggesting that IAMH1 and IAMH2 are the main enzymes responsible for converting IAM into indole-3-acetic acid(IAA)in Arabidopsis.The iamh double mutants did not display obvious developmental defects,indicating that IAM does not play a major role in auxin biosynthesis under normal growth conditions.Our findings provide a solid foundation for clarifying the roles of IAM in auxin biosynthesis and plant development. | Yangbin Gao Xinhua Dai Yuki Aoi Yumiko Takebayashi Liping Yang Xiaorui Guo Qiwei Zeng Hanchuanzhi Yu Hiroyuki Kasahara Yunde Zhao | 2020 | Journal of Genetics and Genomics2020，47，3： | 0 |
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