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| 1 | Study of the mechanisms of acupuncture and moxibustion treatmentfor ulcerative colitis ratsinview of the gene expression of cytokines显示文摘AIM To observe the effect of acupuncture and moxibustion on the expression of IL 1β and IL 6 mRNA in ulcerative colitis rats. METHODS The SD rat ulcerative colitis model was created by immunological method associated with local stimulation. Colonic mucosa was prepared from human fresh surgical colonic specimens, homogenized by adding appropriate amount of normal saline and centrifuged at 3000*!r/*!min . The supernatant was collected for measurement of protein conentration and then mixed with Freund adjuvant. This antigen fluid was first injected into the plantae of the model group rats, and then into their plantae, dorsa, inguina and abdominal cavities (no Freund adjuvant for the last injection) again on the 10th, 17th, 24th and 31st day. When a certain titer of serum anti colonic antibody was reached, 2% formalin and antigen fluid (no Freund adjuvant) were administered separately by enema. The ulcerative colitis rat model was thus set up. The animals were randomly divided into four groups: model control group (MC, n =8), electro acupuncture group (EA, n =8), herbs partition moxibustion group (HPM 8), normal control group (NC, n =8). HPM: Moxa cones made of refined mugwort floss were placed on the medicinal pad (medicinal pad dispensing: Radix Aconiti praeparata, cortex Cinnamomi, etc ) for Qihai (RN 6) and Tianshu (ST 25, bilateral) and ignited. Two moxa cones were used for each acupoint once a day and 14 times in all. EA: Tianshu (bilateral) and Qihai were stimulated by the intermittent pulse with 2Hz frequency, 4mA intensity for 20 minutes once a day and 14 times in all. After treatment, rats of all four groups were killed simultaneously. The spleen was separated and the distal colon was dissected. Total tissue RNA was isolated by the guanidinium thiocyanate phenol chloroform extraction method. RT PCR technique was used to study the expression of IL 1β and IL 6 mRNA. RESULTS IL 1β and IL 6 mRNAs were not detected in the spleen and colonic mucosa of the NC rats, whereas they were significantly expressed in that of the MC rats. IL 1β and IL 6 mRNAs were markedly lower in the EA and HPM rats than that in MC rats. There was no significant difference between the levels of IL 1β and IL 6 mRNAs in the EA and HPM rats. The expressions of IL 1β and IL 6 mRNAs were nearly the same in the spleen and colon of all groups. CONCLUSION Acupuncture and moxibustion greatly inhibited the expression of IL 1β and IL 6 mRNA in the experimental ulcerative colitis rats. | Wu HG Zhou LB Pan YY Huang C Chen HP Shi Z Hua XG | 1999 | World Journal of Gastroenterology1999，5，6： | 45 |
| 2 | Angiostrongyliasis, mainland China 显示文摘 | XG Chen H Li ZR Lun | 2005 | EmergInfect Dis2005，11，10： | 1 |
| 3 | Quality evaluation of Radix Astra- gali from different sources in China显示文摘 | Liu J Chen HB Du XG | 2009 | J Chin Pharm Sci2009，18，： | 1 |
| 4 | High level expression and purification of immunogenic recombinant SAG1 (P30) of Toxoplasma gondii in Escherichia coli 显示文摘 | Chen XG Gong Y Li H | 2001 | Protein Expression Purification2001，23，： | 1 |
| 5 | Nuclear DAMP complex-mediated RAGE-dependent macrophage cell death 显示文摘 | Chen R Fu S Fan XG | 2015 | Biochem Biophys Res Commun2015，458，3： | 1 |
| 6 | Thermal and chemical stability of fluoro-hydroxyapatite ceramics with different fluorine contents 显示文摘 | Chen YM Miao XG | 2005 | Biomaterials2005，26，5： | 1 |
| 7 | Identification and determination of active components in Angelica dahurica Benth and its medicinal preparation by capillary electrophoresis 显示文摘 | WANG KT LIU HT CHEN XG | 2001 | Talanta2001，54，4： | 1 |
| 8 | Human marrow stromal cell therapy for stroke in rat:neurotrophins and functional recovery显示文摘 | Li Y Chen J Chen XG | 2002 | Neurology2002，59，： | 1 |
| 9 | Pyrrolizidine alkaloid clivofine inhibits human normal liver L-02 cells growth and activates p38 mitogen-activated protein klnase in L-02 cells 显示文摘 | Ji LL Zhao XG Chen L | 2002 | Toxices2002，40，12： | 1 |
| 10 | Site-specifkc bone loss in senescenceaccelerated mouse (SAMP6):A mufine model for senile osteoporosis显示文摘 | Chen HY Zhou XG | | 0，，03： | 1 |
| 11 | Protein Purification via Aqueous Two-Phase Extraction (ATPE) and Immobilized Metal Affinity Chromatography. Effectiveness of Salt Addition To Enhance Selectivity and Yield of GFPuv显示文摘 | Li Y Chen J CHEN XG | | 0，，： | 1 |
| 12 | In vitro studies on cellular and molecular mechanisms of arsenic trioxide in the treatment of acute promyelocytic leukemia: A%03 induces NB4 cell apoptosis with downregulation of bcl - 2 expression and modulation of PML - RAR alpha/PML protein显示文摘 | Chen GO Zhu J Shi XG | 1996 | Blood1996，88，： | 1 |
| 13 | High prevalence of KPC-2 car- bapenemase coupled with CTX-M-type extended-spectrumβ-1actamase in carbapenem-resistant Klebsiella pneumoniae in a teaching hospital in China 显示文摘 | Chen SD Hu FP Xu XG | 2011 | Antimieroh Agents ehemother2011，55，5： | 1 |
| 14 | Human marrow stromal cell therapy for stroke in rats:neurotrophins and functional recovery显示文摘 | Li Y Chen J Chen XG | 2002 | Neurology2002，59，4： | 1 |
| 15 | Computerized Tomography of Liver in Aalveolar Echinococcosis Treated with Albendazole显示文摘 | Liu YH Wang XG Chen YT | 1993 | Zhonghua Nei Ke Za Zhi1993，32，11： | 1 |
| 16 | Human marrow stromal cell therapy for stroke in rat:neurotrophins and functional recovery显示文摘 | Li Y Chen J Chen XG | 2002 | Neurology2002，59，： | 1 |
| 17 | The Anophelesgambiae vitellogenin gene (VGT2) promoter directs persistent accumulation of a reporter gene product in transgenic Anopheles stephensi following multiple bloodmeals显示文摘 | Chen XG Marinotti O James AA | 2007 | Am J Trop Med Hyg2007，76，6： | 1 |
| 18 | Human Marrow Stromal Cell Therapy for Stroke in Rat:Neurotrophins and Functional Recovery显示文摘 | Li Y Chen J Chen XG Wang L | 2002 | Neurology (S0028 - 3878)2002，59，4： | 1 |
| 19 | Separation and identification of compounds in Rhizoma Chuanxiong by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry 显示文摘 | Chen XG Kong L Su XY | 2004 | J Chromatogr A2004，1040，2： | 1 |
| 20 | In vitro studies on cellular and molecular mechanisms of arsenic trioxide (As2O3)in the treatment of acute promyelocytic leukemia:As2O3 induces NB4 cell apoptosis with downregulation of Bcl 2 expression and modulation of PML RAR alpha /PML proteins显示文摘 | Chen GQ Zhu J Shi XG | 1996 | Blood1996，88，3： | 1 |
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