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| 1 | Induction of apoptosis by arsenic trioxide and hydroxycamptothecin in gastric cancer cells in vitro显示文摘AIM To study the effects of arsenic trioxide andHCPT on different degrees of differentiated gastriccancer cells(SGC-7901,MKN-45,MKN-28)withrespect to both cytotoxicity and induction ofapoptosis in vitro.METHODS The cytotoxicity of As2O3 and HCPTon gastric cancer cells was determined by MTTassay.Morphologic changes of apoptosis ofgastric cancer cells were observed by lightmicroscopy and transmission electron microscopy.Apoptosis and cell cycle changes of gastric cancercells induced by HCPT and As2O3 were investigatedby TUNEL method and flow cytometry.RESULTS As2O3 and HCPT had remarkablecytotoxic effects on different degrees ofdifferentiated gastric cancer cells.The IC50ofAs2O3 on well differentiated gastric cancer cellMKN-28,moderately differentiated gastric cancercell SGC-7901,and poorly differentiated gastriccancer cell MKN-28 were 8.91 μmol/L,10.57μmol/L,and 11.65 μmol/L,respectively.The IC50of HCPT on MKN-28,SGC-7901,and MKN-45 were9.35 mg/L,10.21 mg/L,and 12.63 mg/Lrespectively after 48 h treatment.After 12 h ofexposure to both drugs,gastric cancer cellsexhibited morphologic features of apoptosis,including cell shrinkage,nuclear condensation, and formation of apoptotic bodies.A typicalsubdiploid peak before G0/G1 phase was observedby flow cytometry.The apoptotic rates of SGC-7901,MKN-45,and MKN-28 were 13.84%,22.52%,and 9.68%,respectively after 48 hexposure to 10 μmol/L As2O3.The apoptotic ratesof SGC-7901,MKN-45,and MKN-28 were 21.88%,12.35%,and 30.26%,respectively after 48 hexposure to 10 mg/L HCPT.The apoptotic indicewere 7%-15% as assessed by TUNEL method.The effect of As2O3 on SGC-7901 showedremarkable cell cycle specificity,which inducedcell death in G1 phase,and blocked G2/M phase.HCPT also showed a remarkable cell cyclespecificity,by inducing cell death and apoptosis inG1 phase and arrest of proliferation at S phase.CONCLUSION As2O3 and HCPT exhibitsignificant cytotoxicity on gastric cancer cells byinduction of apoptosis.As2O3 and HCPT mighthave a promising prospect in the treatment ofgastric cancer,which needs to be further studied. | Tu SP Zhong J Tan JH Jiang XH Qiao MM Wu YX Jiang SH | 2000 | World Journal of Gastroenterology2000，6，4： | 43 |
| 2 | Dietary factors and smoking as risk factors for PD in a rural population in China: a nested case-control study显示文摘 | Ma L Zhang L Gao XH Chen W Wu YP Wang Y Li XF Como P Kieburtz K Qiao YL | 2006 | 中国生物学文摘2006，20，8： | 13 |
| 3 | Association between adiponectin concentrations and diabetic retinopathy in patients with type 2 diabetes: a meta analysis显示文摘 | Fan XH Wu QH Hao YH Ning N Kang Z Cui Y Liu RH | 2014 | Chinese Medical Journal2014，，4： | 8 |
| 4 | Association of polymorphisms in the vascular endothelial growth factor gene and its serum levels with diabetic retinopathy in Chinese patients with type 2 diabetes: A cross-sectional study显示文摘 | Fan XH Wu QH Li Y Hao YH Ning N Kang Z Cui Y Liu RH | 2014 | Chinese Medical Journal2014，，4： | 4 |
| 5 | First-principles simulations of iron with nitrogen:from surface adsorption to bulk diffusion显示文摘Adsorption,absorption and diffusion pathways of nitrogen are studied for ferromagnetic body-centered cubic iron via spin-polarized density functional theory in combination with the climbing image nudged elastic band method.The computed data suggest that,depending on the coverage of N atoms,N prefers to stay on particular surface sites.Once pinned down well below the surface,N prefers to move into octahedral interstices rather than tetrahedral interstices.However,the tetrahedral interstices are crucial because they act as transition states and yield the saddle point energies of the corresponding minimum energy pathways.In comparison with carbon,we found that nitrogen prefers a different pathway from the(1 0 0)surface to the subsurface due to its strong repulsive interaction with Fe ions. | MH Wu XH Liu 顾剑锋 ZH Jin | 2013 | 功能材料信息2013，10，4： | 3 |
| 6 | Delayed cardioprotection of aged rat myocytes is associated with activation of mitogenactivated protein kinase 显示文摘 | Liu XH Wang SW Wu XD | 2000 | Chinese Medical Journal2000，113，1： | 2 |
| 7 | Ab initio molecular orbital study of structure on aluminium borides AlB_n (n = 1- 5) series显示文摘RECENTLY,great attention has been paid to the physical and chemical properties of atomic andsemiconductor clusters.On the properties of AlB_n atomic cluster,experimental studieshave been reported,yet theoretical studies have not been seen.Aluminium borides(AlB_n)have been widely used to change the fiber strength in mechanical engineering and to adjust | Wu, HS Xu, XH Zhou, WL | 1997 | Chinese Science Bulletin1997，42，11： | 2 |
| 8 | Clinical observation on YiSuiShengXueGranule on treating 156 patients with thalassemia major and the molecular mechanism study显示文摘 | Wu ZK Zhang XH | 2007 | Biol Pharm Bull2007，30，11： | 1 |
| 9 | Dihydroartemisinin inhibits angiogenesis induced by multiple myeloma RPMI8226 cells under hypoxic conditions via downregulation of vascular endothelial growth factor expression and suppression of vascular endothelial growth factor secretion显示文摘 | Wu XH Zhou HJ Lee J | 2006 | Anticancer Drugs2006，17，7： | 1 |
| 10 | Effect of exenatide on inflammatory and oxidative stress markers in patients with type 2 diabetes mellitus显示文摘 | Wu JD Xu XH Zhu J | 2011 | Diabetes Technol Ther2011，13，2： | 1 |
| 11 | Calreticulin downregula- tion is associated with FGF-2-induced angiogenesis through calcineurin pathway in ischemic myocardium 显示文摘 | Liu XH Wu XD Cai LR | 2008 | Shock2008，29，1： | 1 |
| 12 | Therapeutic efficacy of bipolar plasmakinetic technique compared with transurethral resection on benign prostate hyperplasia 显示文摘 | Wu WJ Wang XH Wang HP | 2005 | Zhonghua Yi Xue Za Zhi2005，85，47： | 1 |
| 13 | Expression of β1,3-N-acetylglucosaminy ltransferases during differentiation of human acute myeloid leukemia cells显示文摘 | Qiu H Wu SL Guo XH | | 0，，1： | 1 |
| 14 | Effects of polydatin on ALT, AST,TNF- alpha, and COX- 2 in sepsis model mice显示文摘 | Li XH Wu M J Zhang LN | 2013 | Zhong- guo Zhong Xi Yi Jie He Za Zhi2013，33，2： | 1 |
| 15 | Identification and character- ization ofSchistosomajaponicum Sjp40, a potential antigen candi- date for the early diagnosis of schistosomiasis 显示文摘 | Zhou XH Wu JY Huang XQ | 2010 | Diagn Microbiol Infect Dis2010，67，4： | 1 |
| 16 | Role of c-Src in the regulation of vascular contraction and Ca^2+ signaling by angiotensin II in human vascular smooth muscle cells 显示文摘 | Touyz RM Wu XH | 2001 | J Hypert2001，19，3： | 1 |
| 17 | Clinicopathologic characteristics of u- terine adenomyoma in pregnant women显示文摘 | Wang JH He XH Wu R J | 2007 | Fertil Steril2007，88，1： | 1 |
| 18 | Hypoxic precondition- ing induces delayed cardioprotection through p38 MAPK- mediated ealreticulin upregulation 显示文摘 | Wu XD Liu XH Zhu XM | 2007 | Shock2007，27，5： | 1 |
| 19 | A novel low power loss IGBT (LPL-IGBT) and its simulation 显示文摘 | WU Y LU XH KANG B W | 2001 | Chinese Journal of Semiconductor2001，22，12： | 1 |
| 20 | Mercury in urban soils with various types of land use in Beijing,China显示文摘 | Chen X Xia XH Wu S | | 0，，： | 1 |
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