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| 1 | 中国宫颈癌筛查未来之路——细胞学初筛的弃或守显示文摘宫颈癌是迄今人类惟一病因明确的恶性肿瘤,同时也是可直接观察与取材并早期发现的肿瘤。然而,历经数十年的努力,宫颈癌的发生并未得到有效遏制,其发病率近年在女性恶性肿瘤中高居第2位。世界卫生组织统计,全球每年有宫颈癌新发病例52.9万,死亡病例约20万,其中90%以上来自发展中国家。据2015年肿瘤登记年报统计:中国宫颈癌发病率为12.96/10万,死亡率为2.60/10万. | 王轶英 王悦 乔友林 孔北华 Wenxin Zheng | 2017 | 中国实用妇科与产科杂志2017，33，3： | 68 |
| 2 | Preliminary results pertaining to coseismic displacement and preseismic strain accumulation of the Lushan M_S7.0 earthquake,as reflected by GPS surveying显示文摘This paper presents the coseismic displacement and preseismic deformation fields of the Lushan MS7.0 earthquake that occurred on April 20,2013.The results are based on GPS observations along the Longmenshan fault and within its vicinity.The coseismic displacement and preseismic GPS results indicate that in the strain release of this earthquake,the thrust rupture is dominant and the laevorotation movement is secondary.Furthermore,we infer that any possible the rupture does not reach the earth’s surface,and the seismogenic fault is most likely one fault to the east of the Guanxian-Anxian fault.Some detailed results are obtainable.(1)The southern segment of the Longmenshan fault is locked preceding the Lushan earthquake.After the Wenchuan earthquake,the strain accumulation rate in the southeast direction accelerates in the epicenter of the Lushan earthquake,and the angle between the principal compressional strain and the seismogenic fault indicates that a sinistral deformation background in the direction of the seismogenic fault precedes the Lushan earthquake.Therefore,it is evident that the Wenchuan MS8.0 earthquake accelerated the pregnancy of the Lushan earthquake.(2)The coseismic displacements reflected by GPS data are mainly located in a region that is 230 km(NW direction)×100 km(SW direction),and coseismic displacements larger than 10 mm lie predominantly in a100-km region(NW direction).(3)On a large scale,the coseismic displacement shows thrust characteristics,but the associated values are remarkably small in the near field(within 70 km)of the earthquake fault.Meanwhile,the thrust movement in this70-km region does not correspond with the attenuation characteristics of the strain release,indicating that the rupture of this earthquake does not reach the earth’s surface.(4)The laevorotation movements are remarkable in the 50-km region,which is located in the hanging wall that is close to the earthquake fault,and the corresponding values in this case correlate with the attenuation characteristics of the strain release. | WU YanQiang JIANG ZaiSen WANG Min CHE Shi LIAO Hua LI Qiang LI Peng YANG YongLin XIANG HePing SHAO ZhiGang WANG WuXing WEI WenXin LIU XiaoXia | 2013 | Chinese Science Bulletin2013，58，28： | 33 |
| 3 | 中国子宫颈癌及其癌前病变诊治的若干问题显示文摘子宫颈癌是发生于子宫颈阴道部及子宫颈管上皮的恶性肿瘤,其发病率仅次于乳腺癌,列居女性恶性肿瘤第2位。子宫颈癌是由高危型人类乳头瘤病毒(HPV)的持续感染所致,经子宫颈上皮内瘤变(CIN)发展而来。2002年全球子宫颈癌新发病例49.3万人,死亡27.3万人。 | 孙北华 Wenxin Zheng | 2010 | 中华医学杂志2010，90，43： | 24 |
| 4 | Study on the flow field and concentration characteristics of the multiple tandem jets in crossflow显示文摘The characteristics of single and multiple tandem jets(n=2,3,4) in crossflow have been investigated using the realizable k-ε model.The results of this model agree well with experimental measurements using PIV(Particle Image Velocimetry) or LIF(Laser Induced Fluorescence).We analyzed the calculated results and obtained detailed properties of velocity and concentration of the multiple jets in the pre-merging and post-merging regions.When the velocity ratio is identical,the bending degree of the leading jet is greater than that of the rear jets.The last jet penetrates deeper as the jet number increases,and the shielding effect of the front jet declines with jet spacing increase.Interaction of the jet and crossflow induces formation and development of a counter-rotating vortex pair(CVP).CVP makes the distribution of concentration appear kidney-shaped(except in the merging region),and maximum concentration is at the center of the counter-rotating vortex.Concentration at the CVP center is 1.03-1.4 times that of the local jet trajectory.Post-merging velocity and concentration characteristics differ from those of the single jet because of the shielding effect and mixing of all jets.This paper presents a unified formula of trajectory,concentration half-width and trajectory dilution,by introducing a reduction factor. | LI ZhiWei HUAI WenXin QIAN ZhongDong | 2012 | Science China(Technological Sciences)2012，55，10： | 22 |
| 5 | Numerical simulation of sandy bed erosion by 2D vertical jet显示文摘A two-dimensional numerical model of vertical jet scour was developed based on the turbulent flow theory and jet scour mechanism. In this model,drag force acts as the main reference variable and the critical Shields number acts as the incipient motion criteria of sediment. The morphological change in the bed caused by bed-load transport is simulated using the moving mesh method and the suspended-load is not considered. An experiment investigating vertical clear water jet scour was conducted in the laboratory,and some effective experimental results,such as flow patterns and distribution characteristics of scour pits,were obtained. Numerical simulation of the 2D jet scour was conducted using the same parameters as were used in the flume experiment. The evolution process of the jet scour observed in the experiment was simulated by the new model;validation of the numerical model and the algorithm was conducted. Semi-empirical formulas of the characteristic length of the equilibrium scour hole were deduced based on the results of the experiment and simulation. | HUAI WenXin WANG ZengWu QIAN ZhongDong HAN YaQiong | 2011 | Science China(Technological Sciences)2011，54，12： | 21 |
| 6 | Special Section:SARS-CoV-2 Preliminary evidence from a multicenter prospective observational study of the safety and efficacy of chloroquine for the treatment of COVID-19显示文摘Effective therapies are urgently needed for the SARS-CoV-2 pandemic.Chloroquine has been proved to have antiviral effect against coronavirus in vitro.In this study,we aimed to assess the efficacy and safety of chloroquine with different doses in COVID-19.In this multicenter prospective observational study,we enrolled patients older than 18 years old with confirmed SARS-CoV-2 infection excluding critical cases from 12 hospitals in Guangdong and Hubei Provinces.Eligible patients received chloroquinephosphate s00 mg,orally,once(half dose)or twice(full dose)daily.Patients treated with non-chloroquine therapy were included as historical controls.The primary endpoint is the time to undetectable viral RNA.Secondary outcomes include the proportion of patients with undetectable viral RNA by day 10 and 14,hospitalization time,duration of fever,and adverse events.A total of 197 patients completed chloroquine treatment,and 176patients were included as historical controls.The median time to achieve an undetectable viral RNA was shorter in chloroquine than in non-chloroquine(absolute difference in medians-6.0 days;95%CI-6.0 to—4.0).The duration of fever is shorter in chloroquine(geometric mean ratio 0.6;95%CIO.5 to 0.8).No serious adverse events were observed in the chloroquine group.Patients treated with half dose experienced lower rate of adverse events than with full dose.Although randomized trials are needed for further evaluation,this study provides evidence for safety and efficacy of chloroquine in COVID-19 and suggests that chloroquine can be a cost-effective therapy for combating the COVID-19 pandemic. | Mingxing Huang Man Li Fei Xiao Pengfei Pang Jiabi Liang Tiantian Tang Shaoxuan Liu Binghui Chen Jingxian Shu Yingying You Yang Li Meiwen Tang Jianhui Zhou Guanmin Jiang Jingfen Xiang Wenxin Hong Songmei He Zhaoqin Wang Jianhua Feng Changqing Lin Yinong Ye Zhilong Wu Yaocai Li Bei Zhong Ruilin Sun Zhongsi Hong Jing Liu Huili Chen Xiaohua Wang Zhonghe Li Duanqing Pei Lin Tian Jinyu Xia Shanping Jiang Nanshan Zhong Hong Shan | 2020 | National Science Review2020，7，9： | 20 |
| 7 | LncRNA PVT1 promotes ovarian cancer progression by silencing miR-214显示文摘Objective: Emerging evidence indicates that long non-coding RNAs(lnc RNAs) are critical in carcinogenesis and progression of ovarian cancer. This study aimed to explore the functions and molecular mechanisms of plasmacytoma variant translocation I(PVT1) in ovarian cancer Methods: PVT1 and miR-214 were detected by qRT-PCR assays in ovarian cancer tissues and cells. The cell proliferation,migration, and invasion abilities were detected by cell functional experiments, respectively. Western blot assay was performed to detect epithelial-mesenchymal transition(EMT) markers. MiR-214 expression regulated by PVT1 was studied by RNA immunoprecipitation(RIP) and chromatin immunoprecipitation(Ch IP) assays.Results: The expression of PVT1 was up-regulated in ovarian cancer tissues and cell lines. Elevated PVT1 expression was associated with advanced stage and indicated poor prognosis for ovarian cancer patients. The knockdown of PVT1 impaired SKOV3 cell proliferation, migration, and invasion in vitro. The promotion of ovarian cancer progression by PVT1 involved in regulation of the epithelial-mesenchymal transition process and PVT1 interaction with EZH2 represses miR-214 expression in ovarian cancer cells.Conclusions: PVT1 plays an important role in ovarian cancer tumorigenesis, which might be as a novel diagnostic marker and therapeutic target for ovarian cancer. | Ying Chen Hui Du Lewen Bao Wenxin Liu | 2018 | Cancer Biology & Medicine2018，15，3： | 18 |
| 8 | Tapped Inductor Quasi-Z-source Inverters显示文摘提出一种高升压比的单级升压逆变器,它在传统准z源逆变器拓扑中引入抽头电感无源网络,因此称为“抽头电感准z源逆变器”(tapped inductor quasi-Z-source inverter,TL-qZSI)。该抽头电感无源网络包括一个抽头电感和两个二极管,替代了原准Z源逆变器中的一个电感。类似于抽头电感Z源逆变器(tapped inductor Z-source inverter,TL-ZSI),所提出的逆变器也能提供高升压能力,但比TL-ZSI略低些。分析电路的工作机理、升压能力和控制策略,讨论设计上的考虑。最后通过基于简单升压控制方法的实验结果验证该拓扑的实际性能。 | ZHOU Yufei HUANG Wenxin ZHAO Jianwu ZHAO Ping | 2012 | 中国电机工程学报2012，32，27： | 17 |
| 9 | HPV分型与定量检测在子宫颈癌筛查中的作用及意义显示文摘子宫颈癌是截止目前人类唯一病因明确的恶性肿瘤,同时也是可早期发现并能有效预防的恶性肿瘤,但我国目前并未使子宫颈癌的发病率及死亡率得到有效控制,反而有持续走高趋势。本文对以往的子宫颈癌筛查模式重新审视,以期建立适合中国国情的筛查策略,提高筛查的精确性和有效性,缩小与欧美国家的差距。随着分子生物学技术及对HPV研究的不断进展,发现HPV分型及定量检测具有一定的临床价值及意义,以此为核心,探讨子宫颈癌筛查的相关问题,HPV检测是否优于细胞学检查、后HPV疫苗时代HPV分型及定量检测的临床价值及意义等。 | 王悦 王轶英 谢幸 Zheng Wenxin 孔北华 | 2019 | 中华妇产科杂志2019，54，5： | 17 |
| 10 | Dihydrotanshinone I inhibits angiogenesis both in vitro and in vivo显示文摘Dihydrotanshinone 我(DI ) ,从鼠尾草植物 miltiorrhiza Bunge 提取的自然地发生的混合物,被报导了有 cytotoxicity 到许多肿瘤房间。在这研究,我们在人的脐的静脉 endothelial 房间调查了它的 anti-angiogenic 能力。DI 导致了有势力 cytotoxicity 到人的脐的静脉 endothelial 房间,与约 1.28 亩 g/ml 的 IC50 价值。在 0.25-1 亩 g/ml, DI dose-dependently 压制了分别地愈合弯屈, Transwell 侵略和 Matrigel 试管形成试金检测的人的脐的静脉 endothelial 房间移植,侵略,和试管形成。而且,在在小鸡胚胎 chorioallantoic 的 vivo anti-angiogenic 活动重要的显示出的 DI 膜试金。DI 在 0.2 亩 g/egg 导致了微容器密度的 61.1% 禁止的率。总起来说,我们的结果证明 DI 能通过压制 endothelial 房间增长,移植,侵略和试管形成禁止 angio 开始,显示 DI 有一个潜力作为一个新奇 anti-angiogenic 代理人被发展。 | Weipeng Bian Fei Chen Ling Bai Ping Zhang Wenxin Qin | 2008 | Acta Biochimica et Biophysica Sinica2008，40，1： | 13 |
| 11 | Analyzing Industrial Structure Evolution of Old Industrial Cities Using Evolutionary Resilience Theory:A Case Study in Shenyang of China显示文摘The recession and revitalization of old industrial cities concerns urban industrial evolution and its characteristics. Based on the theory of evolutionary resilience, we developed an analytical framework for the industrial structure evolution of old industrial cities, and applied the framework to a case study in Shenyang. The following conclusions are drawn. First, since 1978, Shenyang's industrial growth capacity has shown fluctuation between ‘contraction-expansion'. As the secondary industry has a much stronger expansionary and contractionary capacity for growth, this results in lacking stability leading to industrial structure transformation. Second, since 1999, the orientation towards a high-end manufacturing industry in Shenyang has weakened, and the evolution of the new and old growth path is characterized by low-end orientation. Third, since 2007, Shenyang's industrial innovation output capacity has dropped sharply which has been significantly affected by scientific and technological personnel and enterprise-owed science and technology institutions and to a less extent by R&D expenditure. We applied the resilience theory to study the industrial evolution of an old industrial city, explored new study perspectives on industrial evolution and verified the applicability of the resilience theory. This paper provides a scientific reference for understanding the recent deceleration in economic growth in the Northeast old industrial base, and for exploring new paths toward revitalization. | GUAN Haoming LIU Wenxin ZHANG Pingyu LO Kevin LI Jing LI Liangang | 2018 | Chinese Geographical Science2018，28，3： | 12 |
| 12 | Hydrothermal Dolomite in the Upper Sinian (Upper Proterozoic) Dengying Formation, East Sichuan Basin, China显示文摘Hydrothermal Dolomite(HTD) is present in the Upper Sinian(Upper Proterozoic) Dengying Formation, east Sichuan Basin, China. The strata are comprised by primary dolomite. The HTD has various textures, including zebra dolomite, subhorizontal sheet-like cavities filled by saddle dolomite and breccias cemented by saddle dolomites as well occur as a fill of veins and fractures. Also co-occur MVT type lead-zinc ores in the study area. The δ13C and δ18O isotopes of HTD in the Upper Sinian Dengying Formation are lighter than those of the host rocks, while 87Sr/86 Sr is higher. The apparent difference in carbon, oxygen and strontium isotopes, especially the large difference in 87Sr/86 Sr isotopes ratio indicate crystallization from hot basinal and/or hydrothermal fluids. Saddle dolomite was precipitated at temperatures of 270-320°C. The diagenetic parasequences of mineral assemblage deposited in the Dengying Formation are:(1) dolomite host rock →sphalerite-galena-barite-fluorite;(2) dolomite host rock →saddle dolomite →quartz;(3) dolomite host rock → saddle dolomite→bitumen;(4) dolomite host rock → saddle dolomite → barite. The mean chemical composition of the host dolomite matrix and HTD didn't change much during hydrothermal process. The fluids forming the HTDs in the Dengying Formation were mixtures of freshwater from the unconformity at the top of Sinian, fluids from diagenetic compaction and hydrocarbon generation & expulsion from the Lower Cambrian Niutitang Formation mudstones or the Doushantuo Formation silty mudstones, and hydrothermal fluids from the basement. The hydrocarbon reservoirs associated with the HTD were mostly controlled by the basement faults and fractures and karsting processes at the unconformity separating Sinian and Cambrian strata. The hydrocarbon storage spaces of HTD included dissolved cavities and intercrystalline pores. Dissolution cavities are extensive at the top of Dengying Formation, up to about 46 m below the unconformity between Sinian and Cambrian and were generated mainly during karstification. Hydrothermal alteration enhanced the reservoir property of the Dengying Formation dolomites with 3%-5% increase in porosity. No agreement has been reached why zebra dolomite occurs only in the Upper Sinian strata, which would indicate that HTD mineralization occurred during two different periods, each of them related to major extensional tectonic event. The early one related to the Xingkai taphrogenesis(Z2-?1)and the later one to the Emei taphrogenesis(D2-T2). But, all the data from saddle dolomite suggest that the predominant crystallization occurred during the latter event. | LIU Shugen HUANG Wenming JANSA Luba F. WANG Guozhi SONG Guangyong ZHANG Changjun SUN Wei MA Wenxin | 2014 | Acta Geologica Sinica(English Edition)2014，88，5： | 11 |
| 13 | 子宫内膜癌病理研究进展及临床意义显示文摘子宫内膜癌是女性生殖道最常见的恶性肿瘤之一,发病率呈不断上升趋势。近年来关于其发病机制、分子遗传学改变、恶性前期病变、组织学分类等领域的研究均取得很大进展。本文选择有临床意义的病理研究进展并结合国内实际情况作一评述。 | 李丽 郭东辉 王轶英 ZHENG Wenxin | 2015 | 实用妇产科杂志2015，31，11： | 11 |
| 14 | Biomechanical comparison of locking plate and crossing metallic and absorbable screws fixations for intra-articular calcaneal fractures显示文摘The locking plate and percutaneous crossing metallic screws and crossing absorbable screws have been used clinically to treat intra-articular calcaneal fractures, but little is known about the biomechanical differences between them. This study compared the biomechanical stability of calcaneal fractures fixed using a locking plate and crossing screws. Three-dimensional finite-element models of intact and fractured calcanei were developed based on the CT images of a cadaveric sample. Surgeries were simulated on models of Sanders type III calcaneal fractures to produce accurate postoperative models fixed by the three implants. A vertical force was applied to the superior surface of the subtalar joint to simulate the stance phase of a walking gait. This model was validated by an in vitro experiment using the same calcaneal sample. The intact calcaneus showed greater stiffness than the fixation models. Of the three fixations, the locking plate produced the greatest stiffness and the highest von Mises stress peak. The micromotion of the fracture fixated with the locking plate was similar to that of the fracture fixated with the metallic screws but smaller than that fixated with the absorbable screws. Fixation with both plate and crossing screws can be used to treat intra-articular calcaneal fractures. In general, fixation with crossing metallic screws is preferable because it provides sufficient stability with less stress shielding. | Ming Ni Duo Wai-Chi Wong Jiong Mei Wenxin Niu Ming Zhang | 2016 | Science China(Life Sciences)2016，59，9： | 11 |
| 15 | Preparation and cytocompatibility of polylactic acid/hydroxyapatite/graphene oxide nanocomposite fibrous membrane显示文摘A series of polylactic acid (PLA) based nanocomposite fibrous membranes,including neat PLA,PLA/hydroxyapatite (HA) and PLA/HA/graphene oxide (GO),were fabricated via electrospinning method.The morphology and composition were investigated by scanning electron microscopy (SEM),X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) respectively.The thermal stability was determined by thermogravimetric analysis (TGA).To estimate the cytocompatibility of asprepared PLA/HA/GO fibrous membrane,MC3T3-E1 cells were cultured,and the corresponding cell adhesion and differentiation capability were investigated by fluorescence microscopy,SEM and MTT test.The electrospun ternary PLA/HA/GO membrane exhibited three-dimensional fibrous structure with relatively rough surface morphology,which made itself ideal for cell attachment and proliferation in bone tissue regeneration.The fluorescence microscopy,SEM and MTT test confirmed that the PLA/HA/GO nanocomposite fibrous membrane created a proper environment for the seeding and proliferation of MC3T3-E1 cells. | MA HaiBin SU WenXin TAI ZhiXin SUN DongFei YAN XingBin LIU Bin XUE QunJi | 2012 | Chinese Science Bulletin2012，57，23： | 11 |
| 16 | HPV定量检测的临床意义与研究进展显示文摘子宫颈癌是女性常见的恶性肿瘤之一,也是目前唯一的病因明确的恶性肿瘤.2002年的统计表明,全球每年子宫颈癌的死亡病例数达27万;其中约1/4来自中国.近年,子宫颈癌的发病率和死亡率在上升,且明显趋向年轻化.自1995年国际癌症研究中心(International Agency for Research on Cancer,IARC)正式宣布HPV的某些型别持续感染是导致子宫颈癌的最主要因素以来,迄今已发现HPV的200余种型别,依据致癌性分为高危型及低危型. | 王轶英 王悦 孔北华 Wenxin Zheng | 2017 | 中华妇产科杂志2017，52，8： | 10 |
| 17 | DNA methylation and chromatin accessibility profiling of mouse and human fetal germ cells显示文摘染色质改变为人的初发的细菌房间的 epigenetic reprogramming 是重要的。然而,全面染色质状态还没为人的胎儿的细菌房间(FGC ) 被分析了。这里,我们使用 nucleosome 占有和 methylation 定序方法在人和老鼠在胎儿的细菌房间开发期间在一系列关键时间点分析染色体宽的染色质可接近性和 DNA methylome。我们在人和老鼠 FGC 发现 116 887 和 137 557 个弄空 nucleosome 的区域(NDR ) ,盖住 germline 特定、高度有活力的规章的 genomic 元素的一个大集合,例如 enhancers。而且,我们发现远侧的 NDR 在人的 FGC 为象 NANOG , SOX17 , AP2 和 OCT4 那样的 pluripotency 和细菌房间主人管理者的有约束力的主题明确地被充实,显示在pluripotency相关的基因和细菌之间的精细的规章的平衡的存在在人的 FGC 的房间特定的基因,并且为在 vivo 的细菌房间开发的这些基因的功能的意义。我们的工作为把提供一张全面、高分辨率的路线图染色质状态转变动力学在人和老鼠 FGC 的 epigenomic reprogramming 期间。 | Hongshan Guo Boqiang Hu Liying Yan Jun Yong Yan Wu Yun Gao Fan Guo Yu Hou Xiaoying Fan Ji Dong Xiaoye Wang Xiaohui Zhu Jie Yan Yuan Wei Hongyan Jin Wenxin Zhang Lu Wen FuchouTang Jie Qiao | 2017 | Cell Research2017，27，2： | 9 |
| 18 | Changes of soil microbial communities during decomposition of straw residues under different land uses显示文摘Monitoring soil microbial communities can lead to better understanding of the transformation processes of organic carbon in soil. The present study investigated the changes of soil microbial communities during straw decomposition in three fields, i.e., cropland, peach orchard and vineyard. Straw decomposition was monitored for 360 d using a mesh-bag method. Soil microbial metabolic activity and functional diversity were measured using the Biolog-Eco system. In all three fields, dried straws with a smaller size decomposed faster than their fresh counterparts that had a larger size. Dried corn straw decomposed slower than dried soybean straw in the early and middle stages, while the reverse trend was found in the late stage. The cropland showed the highest increase in microbial metabolic activity during the straw decomposition, whereas the peach orchard showed the lowest. There was no significant change in the species dominance or evenness of soil microbial communities during the straw decomposition. However, the species richness fluctuated significantly, with the peach orchard showing the highest richness and the cropland the lowest. With different carbon sources, the peach orchard utilised carbon the most, followed by the cropland and the vineyard. In all three fields, carbon was utilized in following decreasing order: saccharides>amino acids>polymers>polyamines>carboxylic acids>aromatic compounds. In terms of carbon-source utilization, soil microbial communities in the peach orchard were less stable than those in the cropland. The metabolic activity and species dominance of soil microbial communities were negatively correlated with the straw residual percentage. Refractory components were primarily accumulated in the late stages, thus slowing down the straw decomposition. The results showed that dried and crushed corn straw was better for application in long-term fields. The diversity of soil microbial communities was more stable in cropland than in orchards during the straw decomposition. | ZHANG Hong XU Wenxin LI Yubao LYU Jialong CAO Yingfei HE Wenxiang | 2017 | Journal of Arid Land2017，9，5： | 9 |
| 19 | Metabolomic Profiling in Selaginella lepidophylla at Various Hydration States Provides New Insights into the Mechanistic Basis of Desiccation Tolerance显示文摘Selaginella lepidophylla 是仅仅一些之一发展了干燥忍耐(DT ) 或从一个 air-dried 状态复兴的能力的尖铁苔藓(Selaginellaceae ) 的种类。以便理解 DT 的新陈代谢的基础, S。lepidophylla 受到一五阶段, rehydration/dehydration 周期,然后分析了使用基于 GC/MS 和 UHLC/MS/MS2 平台介绍技术的非偏导的、全球 metabolomics。包括 167 说出的 251 代谢物(66.5%) 的一个总数并且 84 (33.4%) 未命名的混合物被描绘。仅仅 42 (16.7%) 并且(29.5%) 74 混合物显示出显著地增加或减少的丰富分别地,显示大多数混合物组成地被生产包括高度丰富的 trehalose,蔗糖,和葡萄糖。几 glycolysis/gluconeogenesis 和 tricarboxylic 酸(TCA ) 周期中介在 100% 亲戚水内容(RWC ) 和 50% RWC 显示出增加的丰富。Vanillate,一种有势力抗氧化剂,也更充满含水的状态。许多不同的糖白酒和糖酸更充满含水的状态。这些 polyols 多半速度率在水和期间在弄干的过程以及慢水吸收期间浇损失,稳定蛋白质,并且清除反应的氧种类(ROS ) 。相反,充满氮并且 -glutamyl 氨基酸,瓜氨酸,和核苷酸分解代谢产品(例如 allantoin ) 更充满干燥状态,建议这些混合物可能在水和期间或在清除的 ROS 在氮动员起重要作用。紫外保护的混合物象 3-(3-hydroxyphenyl ) 那样 propionate, apigenin,和 naringenin,更充满干燥状态。大多数类脂化合物组成地被生产,与胆碱磷酸盐的例外,它更充满干燥状态并且多半在膜水和和稳定起一个作用。相反,几多元不堡和丰满的酸更充满含水的状态,建议这些混合物多半帮助在脱水期间维持膜流动性。最后, S。lepidophylla 包含了显示了双重或更大的丰富在的七未命名的混合物干燥或 rehydrating 状态,建议这些混合物可能在 DT 起适应作用。 | Abou Yobi Bernard W.M. Wone Wenxin Xu Danny C. Alexander Lining Guo John A. Ryals Melvin J. Oliver John C. Cushman | 2013 | Molecular Plant2013，6，2： | 9 |
| 20 | Clinical applications of MARSALA for preimplantation genetic diagnosis of spinal muscular atrophy显示文摘Conventional PCR methods combined with linkage analysis based on short tandem repeats(STRs) or Karyomapping with single nucleotide polymorphism(SNP) arrays, have been applied to preimplantation genetic diagnosis(PGD) for spinal muscular atrophy(SMA), an autosome recessive disorder. However, it has limitations in SMA diagnosis by Karyomapping, and these methods are unable to distinguish wildtype embryos with carriers effectively. Mutated allele revealed by sequencing with aneuploidy and linkage analyses(MARSALA) is a new method allowing embryo selection by a one-step next-generation sequencing(NGS) procedure, which has been applied in PGD for both autosome dominant and X-linked diseases in our group previously. In this study, we carried out PGD based on MARSALA for two carrier families with SMA affected children. As a result, one of the couples has given birth to a healthy baby free of mutations in SMA-causing gene. It is the first time that MARSALA was applied to PGD for SMA, and we can distinguish the embryos with heterozygous deletion(carriers) from the wild-type(normal) ones accurately through this NGS-based method. In addition, direct mutation detection allows us to identify the affected embryos(homozygous deletion), which can be regarded as probands for linkage analysis, in case that the affected family member is absent. In the future, the NGS-based MARSALA method is expected to be used in PGD for all monogenetic disorders with known pathogenic gene mutation. | Yixin Ren Xu Zhi Xiaohui Zhu Jin Huang Ying Lian Rong Li Hongyan Jin Yan Zhang Wenxin Zhang Yanli Nie Yuan Wei Zhaohui Liu Donghong Song Ping Liu Jie Qiao Liying Yan | 2016 | Journal of Genetics and Genomics2016，43，9： | 9 |
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