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| 1 | Expansion and differentiation of human hepatocyte-derived liver progenitor-like cells and their use for the study of hepatotropic pathogens显示文摘The study of pathophysiological mecha nisms in huma n liver disease has bee n constrained by the in ability to expa nd primary hepatocytes in vitro while maintaining proliferative capacity and metabolic function. We and others have previously shown that mouse mature hepatocytes can be con verted to liver progenitor-like cells in vitro with defined chemical factors. Here we describe a protocol achieving efficient conversion of human primary hepatocytes into liver progenitor-like cells (HepLPCs) through delivery of developmentally relevant cues, including NAD +-dependent deacetylase SIRT1 signaling. These HepLPCs could be expanded sign ifica ntly duri ng in vitro passage. The expa nded cells can readily be con verted back into metabolically functional hepatocytes in vitro and upon tran splantati on in vivo. Under three-dime nsional culture conditions, differentiated cells gen erated from HepLPCs regai ned the ability to support infect io n or reactivatio n of hepatitis B virus (HBV). Our work dem on strates the utility of the con versi on betwee n hepatocyte and liver proge nitor-like cells for studying HBV biology and an tiviral therapies. These fin dings will facilitate the study of liver diseases and regenerative medicine. | Gong-Bo Fu Wei-Jian Huang Min Zeng Xu Zhou Hong-Ping Wu Chang-Cheng Liu Han Wu Jun Weng Hong-Dan Zhang Yong-Chao Cai Charles Ashton Min Ding Dan Tang Bao-Hua Zhang Yi Gao Wei-Feng Yu Bo Zhai Zhi-Ying He Hong-Yang Wang He-Xin Yan | 2019 | Cell Research2019，29，1： | 18 |
| 2 | Distributed processing based fault location,isolation,and service restoration method for active distribution network显示文摘Active distribution network(ADN)is a solution for power system with interconnection of distributed energy resources(DER),which may change the network operation and power flow of traditional power distribution network.However,in some circumstances the malfunction of protection and feeder automation in distribution network occurs due to the uncertain bidirectional power flow.Therefore,a novel method of fault location,isolation,and service restoration(FLISR)for ADN based on distributed processing is proposed in this paper.The differential-activated algorithm based on synchronous sampling for feeder fault location and isolation is studied,and a framework of fault restoration is established for ADN.Finally,the effectiveness of the proposed algorithm is verified via computer simulation of a case study for active distributed power system. | Jiaming WENG Dong LIU Ning LUO Xueyong TANG | 2015 | Journal of Modern Power Systems and Clean Energy2015，3，4： | 16 |
| 3 | Top-dressing nitrogen fertilizer rate contributes to decrease culm physical strength by reducing structural carbohydrate content in japonica rice显示文摘Lodging is an important factor limiting rice yield and quality by bending or breaking stem in japonica rice(Oryza sativa L.) production. The objectives of this study were to determine the mechanism of lodging resistance in japonica rice as affected by carbohydrate components, especially its related arrangement in culm tissue and response to top-dressing nitrogen(N) fertilizer. Field experiments were conducted in Danyang County, Jiangsu Province, China, by using two japonica rice varieties Wuyunjing 23(lodging-resistance variety) and W3668(lodging-susceptible variety) with three top-dressing N fertilizer rates(0, 135 and 270 kg N ha^(–1)) in 2013 and 2014. Lodging related physical parameters, morphological characteristics and stem carbohydrate components were investigated at 30 d after full heading stage. Results showed that with increasing N fertilizer rates, the lodging rate and lodging index increased rapidly primarily due to significant reduction of breaking strength in two japonica rice varieties. Correlation analysis revealed that breaking strength was significantly and positively correlated with bending stress, but negatively correlated with section modulus, except for significant correlation at W3668 in 2014. Higher stem plumpness status and structural carbohydrate contents significantly enhanced stem stiffness, despite of lower non-structural carbohydrate. With higher N fertilizer rate, the culm wall thickness was almost identical, and culm diameter increased slightly. The structural carbohydrates, especially for lignin content in culm, reduced significantly under high N rate. Further histochemical staining analysis revealed that high N treatments decreased the lignin deposition rapidly in the sclerenchyma cells of mechanical tissue, large vascular bundle and small vascular bundle region, which were consistent with reduction of bending stress, especially for W3668 and thus, resulted in poor stem strength and higher lodging index. These results suggested that structural carbohydrate plays a vital role for improving stem strength in japonica rice. N rate decreased lodging resistance primarily due to poor stem stiffness, by reducing structural carbohydrate content and lignin deposition in the secondary cell wall of lower internode culm tissue. | ZHANG Wu-jun WU Long-mei DING Yan-feng WENG Fei WU Xiao-ran LI Gang-hua LIU Zheng-hui TANG She DING Cheng-qiang WANG Shao-hua | 2016 | Journal of Integrative Agriculture2016，15，5： | 14 |
| 4 | Tripartite motif.containing 3(TRIM3)inhibits tumor growth and metastasis of liver cancer显示文摘Background:Reduced expression of tripartite motif-containing 3(TRIM3) has been reported to be involved in the pathogenesis of human glioblastoma.In our previous research,we found that TRIM3 expression was markedly reduced in human primary hepatocellular carcinoma(HCC) tissues and that low TRIM3 expression was associated with short survival of HCC patients.However,the role of TRIM3 in liver cancer remains unknown.This study aimed to investigate the function of TRIM3 in liver cancer cells.Methods:The protein levels of TRIM3 in five liver cancer cell lines(SK-Hep1,Hep3 B,Huh7,HepG2,Bel-7402) and one normal liver cell line(L02) were detected with Western blotting.HepG2 and Bel-7402 cells with low TRIM3 expression were infected with recombinant lentiviruses overexpressing TRIM3(LV-TRIM3),whereas Huh7 and Hep3 B cells with high TRIM3 expression were transfected with TRIM3-targeted small interfering RNA(siTRIM3).The functions of TRIM3 in the proliferation,colony formation,cell cycle,migration,invasion,and apoptosis of the above cell lines were examined.The effect ofTRIM3 on tumor growth and metastases in nude mice was also investigated.Results:TRIM3 was overexpressed in HepG2 and Bel-7402 cells with LV-TRIM3 infection,which further reduced proliferation,colony formation,migration,and invasion of both cell lines.Cell cycle analysis showed that TRIM3 overexpression induced G_0/G_1 phase arrest in HepG2 and Bel-7402 cells.Moreover,apoptosis was not increased in HepG2 or Bel-7402 cells overexpressing TRIM3.Contrarily,silencing TRIM3 expression in Huh7 and Hep3 B cells by siTRIM3 led to significantly decreased percentages of both cells in the G_0/G_1 phase and promoted cell proliferation,colony formation,migration,and invasion.In vivo experiment results confirmed that TRIM3 overexpression suppressed tumor growth and metastasis.Conclusions:TRIM3 plays a tumor-suppressing role in the regulation of liver cancer development by reducing cell proliferation through cell cycle arrest at the G_0/G_1 phase. | Xu.Qiong Huang Xiao.Fei Zhang Jin.Hua Xia Jie Chao Qiu.Zhong Pan Jing.Jing Zhao Zi.Qi Zhou Chang.Long Chen Yan Tang De.Sheng Weng Jian.Hua Zhang Jian.Chuan Xia | 2017 | Chinese Journal of Cancer2017，36，9： | 13 |
| 5 | The mechanism of barrier river reaches in the middle and lower Yangtze River显示文摘在它人工地或自然地被扰乱以后,冲积隧道总是以沉积运输的平衡国家调整了自己。怎么维持沉积运输的平衡国家并且使河政体稳定总是是河的 geomorphologists 的担心。在长江的中间、更低的活动范围的隧道被区分的河和单个线的河的蹒跚的分发描绘。河政体的变化比在单个线的河里在区分的河里猛烈地是更多。区分的河里的河政体的调整是否能通过单个线的河并且宣传到下游的活动范围,影响全面的河政体的稳定性。研究证明障碍河活动范围能阻止宣传到下游的活动范围接近在上游的隧道调整;因此,它在稳定河政体起一个关键作用。这研究在长江的中间、更低的活动范围调查 34 个单个线的河活动范围。根据长江的中间、更低的活动范围的河的进程的系统的摘要,障碍河活动范围的控制因素被总结并且提取:平面形态学单个线、蜿蜒地流;没有流动偏斜,节点在河活动范围的上面或中间的部分散布了;水力的几何系数是不到 4;纵的坡度比 12 大,凹面河岸的泥土内容比 9.5% 大,并且床沉积的中部的直径比 0.158 公里大。从海军司烧方程,流动的弯曲半径的计算公式动态轴被推出,然后这些的角色在限制流动的迁居上控制因素动态轴和障碍河活动范围的形成被分析。当时,障碍河活动范围被看作这到隧道边界的限制力量的动态轴是的流动的迁居力量的比率不到 1 在不同流动层次下面。障碍河活动范围的机制是以便甚至当在上游的河政体调整时,这个活动范围的隧道边界能总是抑制流动的移植振幅动态轴并且在不同的在上游的河政体条件下面集中主要的流线的平面位置,提供相对稳定的到来的流动为下游的活动范围调节,从而阻止宣传到下游的活动范围接近在上游的河政体调整。 | YOU Xingying TANG Jinwu ZHANG Xiaofeng HOU Weiguo YANG Yunping SUN Zhaohua WENG Zhaohui | 2017 | Journal of Geographical Sciences2017，27，10： | 9 |
| 6 | Anterior vs conventional approach right hepatic resection for large hepatocellular carcinoma:A systematic review and meta-analysis显示文摘AIM To compare the clinical outcomes of right hepatectomy for large hepatocellular carcinoma via the anterior and conventional approach.METHODS We comprehensively performed an electronic search of Pub Med, EMBASE, and the Cochrane Library for randomized controlled trials(RCTs) or controlled clinical trials(CCTs) published between January 2000 and May 2017 concerning the anterior approach(AA) and the conventional approach(CA) to right hepatectomy. Studies that met the inclusion criteria were included, and their outcome analyses were further assessed using a fixed or random effects model.RESULTS This analysis included 2297 patients enrolled in 16 studies(3 RCTs and 13 CTTs). Intraoperative blood loss [weighted mean difference =-255.21; 95% confidence interval(95%CI):-371.3 to-139.12; P < 0.0001], intraoperative blood transfusion [odds ratio(OR) = 0.42; 95%CI: 0.29-0.61; P < 0.0001], mortality(OR = 0.59; 95%CI: 0.38-0.92; P = 0.02), morbidity(OR = 0.77; 95%CI: 0.62-0.95; P = 0.01), and recurrencerate(OR = 0.62; 95%CI: 0.47-0.83; P = 0.001) were significantly reduced in the AA group. Patients in the AA group had better overall survival(hazard ratio [HR] = 0.71; 95%CI: 0.50-1.00; P = 0.05) and disease-free survival(HR = 0.67; 95%CI: 0.58-0.79; P < 0.0001) than those in the CA group.CONCLUSION The AA is safe and effective for right hepatectomy for large hepatocellular carcinoma and could accelerate postoperative recovery and achieve better survival outcomes than the CA. | Jian-Xin Tang Jin-Jun Li Rui-Hui Weng Zi-Ming Liang Nan Jiang | 2017 | World Journal of Gastroenterology2017，23，44： | 7 |
| 7 | Capture and electro-splitting of CO_2 in molten salts显示文摘Due to the serious greenhouse gas effects caused by the increasing concentration of atmospheric CO_2,carbon capture and storage(CCS) has been an important area of research and many technologies are developed within this field. Molten salt CO_2 capture and electrochemical transformation(MSCC-ET) process is a desirable method due to a high CO_2 solubility, a wide potential window of molten salts and easily-controlled electrode reactions. Generally, electro-splitting CO_2 in molten salts begins with CO_2 absorption reactions to form CO_3^(2-), which is then followed by the carbon deposition at the cathode and O_2 evolution at the anode. As a result, CO_2 is electro-converted to O_2 and carbon with different morphologies, compositions, microstructures and functional properties. This report introduces the MSCC-ET process, summarizes the reactions occurring in the molten salts and at the electrode surfaces, as well as the morphological variations of the cathodic products. The inert anode materials, cost estimation and scale-up evaluation of the process are then discussed. It is presumed that with a comprehensive understanding of the electrode reactions during electrolysis and the functional properties of carbon materials obtained during CO_2 electro-splitting can provide a foundation for further developing this environmentally friendly process. | Wei Weng Lizi Tang Wei Xiao | 2019 | Journal of Energy Chemistry2019，28，1： | 7 |
| 8 | Effects of Shuanghuanglian oral liquids on patients with COVID-19:a randomized,open-label,parallel-controlled,multicenter clinical trial显示文摘We conducted a randomized,open-label,parallel-controlled,multicenter trial on the use of Shuanghuanglian(SHL),a traditional Chinese patent medicine,in treating cases of COVID-19.A total of 176 patients received SHL by three doses(56 in low dose,61 in middle dose,and 59 in high dose)in addition to standard care.The control group was composed of 59 patients who received standard therapy alone.Treatment with SHL was not associated with a difference from standard care in the time to disease recovery.Patients with 14-day SHL treatment had significantly higher rate in negative conversion of SARS-CoV-2 in nucleic acid swab tests than the patients from the control group(93.4%vs.73.9%,P=0.006).Analysis of chest computed tomography images showed that treatment with high-dose SHL significantly promoted absorption of inflammatory focus of pneumonia,which was evaluated by density reduction of inflammatory focus from baseline,at day 7(mean difference(95%CI),−46.39(−86.83 to−5.94)HU;P=0.025)and day 14(mean difference(95%CI),−74.21(−133.35 to−15.08)HU;P=0.014).No serious adverse events occurred in the SHL groups.This study illustrated that SHL in combination with standard care was safe and partially effective for the treatment of COVID-19. | Li Ni Zheng Wen Xiaowen Hu Wei Tang Haisheng Wang Ling Zhou Lujin Wu Hong Wang Chang Xu Xizhen Xu Zhichao Xiao Zongzhe Li Chene Li Yujian Liu Jialin Duan Chen Chen Dan Li Runhua Zhang Jinliang Li Yongxiang Yi Wei Huang Yanyan Chen Jianping Zhao Jianping Zuo Jianping Weng Hualiang Jiang Dao Wen Wang | 2021 | Frontiers of Medicine2021，15，5： | 7 |
| 9 | Electrochemical Oxidative C-H Sulfenylation of Imidazopyridines with Hydrogen Evolution显示文摘Summary of main observation and conclusion Selective oxidative C-H sulfenylation of imidazopyridine heterocycles is achieved using an undivided electrolytic cell.The reaction avoids the use of stoichiometric amount of external chemical oxidant and produces hydrogen gas as the only byproduct. Both aryl and aliphatic thiols demonstrate good reactivity for C-S bond formation. | Yong Yuan Yangmin Cao Jin qiao Yueping Lin Xiaomei Jiang Yaqing Weng Shan Tang Aiwen Lei | 2019 | Chinese Journal of Chemistry2019，37，1： | 5 |
| 10 | Inadequate glycaemic control and antidiabetic therapy among inpatients with type 2 diabetes in Guangdong Province of China显示文摘背景糖尿病 mellitus 在调查的 China.We 在最近的年里变得流行在来自在广东省教医院的十所大学的类型 2 糖尿病的住院病人之中的多糖症和不适当的 glycaemic 控制的流行,中国。当 HbA1c() 6.5%.Therapeutic 政体包括了没有干预,在糖尿病的病人的方法不适当的 glycaemic 控制被定义,生活方式仅仅,口头的 antiglycemic 代理人(OA ) ,加 OA (insulin+OA ) 的胰岛素,或胰岛素仅仅。Antidiabetic 管理包括了 monotherapy,两倍治疗,三元组或四倍的治疗。在有已知的历史的 493 个糖尿病的住院病人之中的结果, 75%had HbA1c6.5%.Inadequate 葡萄糖控制率更经常比在 OA 政体(71%)( PO.001 )上在 insulin+OA 政体(97%)上在病人被看见,并且在联合治疗(81%-96%)上的病人更经常比 monotherapy (75%)( P0.05 ),胰岛素上的 .Patients 由吝啬的 HbA1c , glycemic 控制率,糖尿病持续时间, microvascular 复杂并发症,和 BMI ( P0.01 )在 OA 上与病人显著地不同。这研究看了类型 2 糖尿病的病人的那 glycaemic 控制的结论为胰岛素的收到的胰岛素和开始时间通常是谁的病人败坏了 delayed.It 依赖临床医生从传统的逐步的治疗搬到更活跃、早的联合 antidiabetic 治疗提供更好的葡萄糖控制。 | BI Yan YAN Jin-hua LIAO Zhi-hong LI Yan-bing ZENG Long-yi TANG Kuan-xiao XUE Yao-ming YANG Hua-zhang LI Lu CAI De-hong WU Ge ZHANG Fan LIN Shao-da XIAO Zheng-hua ZHU Da-long WENG Jian-ping | 2008 | Chinese Medical Journal2008，，8： | 5 |
| 11 | ZBTB20 is involved in liver regeneration after partial hepatectomy in mouse显示文摘BACKGROUND: A better understanding of the molecular mechanisms in liver regeneration holds promise for exploring the new potential therapy for liver failure. The present study was to investigate the role of zinc finger and BTB domain-containing protein 20(ZBTB20), a potential factor associated with liver regeneration, in a model of 70% hepatectomy in mice. METHODS: Parameters for liver proliferation such as liver/body ratio and BrdU positivity were obtained via direct measurement and immunohistochemistry. The levels of zinc fingers and homeoboxes 2(ZHX2), ZBTB20, alpha-fetoprotein(AFP) and glypican 3(GPC3) transcripts in the regenerating liver tissue of a 70% hepatectomy rodent model were monitored by real-time PCR analysis at different time points. Knockdown of ZBTB20 was performed to characterize its regulatory function. RESULTS: A negatively regulating relationship between ZHX2, ZBTB20 and AFP, GPC3 was revealed from 24 to 72 hours after 70% hepatectomy. ZBTB20 appears to negatively regulate AFP and GPC3 transcription since the knockdown of ZBTB20 promoted the proliferation of hepatocytes and the expression of AFP and GPC3. CONCLUSION: In addition to AFP, GPC3 and ZHX2, ZBTB20 is a new regulator in liver regeneration and the decrease of ZBTB20 expression following 70% hepatectomy promotes AFP and GPC3 expression. | Ming-Zhe Weng Peng-Yuan Zhuang Zhen-Yu Hei Pei-Yi Lin Zhi-Sheng Chen Ying-Bin Liu Zhi-Wei Quan Zhao-Hui Tang | 2014 | Hepatobiliary & Pancreatic Diseases International2014，13，1： | 5 |
| 12 | Numerical investigation on the effect of depletion-induced stress reorientation on infill well hydraulic fracture propagation显示文摘Depletion-induced stress change causes the redistribution of stress field in reservoirs,which can lead to the reorientation of principal stresses.Stress reorientation has a direct impact on fracture propagation of infill wells.To understand the effect of stress reorientation on the propagation of infill well’s fractures,an integrated simulation workflow that combines the reservoir flow calculation and the infill well hydraulic fracturing modeling is adopted.The reservoir simulation is computed to examine the relationship between the extent of stress reversal region and reservoir properties.Then,the hydraulic fracturing model considering the altered stress field for production is built to characterize the stress evolution of secondary fracturing.Numerical simulations show that stress reorientation may occur due to the decreasing of the horizontal stresses in an elliptical region around the parent well.Also,the initial stress difference is the driving factor for stress reorientation.However,the bottom hole pressure,permeability and other properties connected with fluid flow control timing of the stress reorientation.The decrease of the horizontal stresses around the parent well lead to asymmetrical propagation of a hydraulic fracture of the infill well.The study provides insights on understanding the influence of stress reorientation to the infill well fracturing treatment and interference between parent and infill wells. | Feng-Shou Zhang Liu-Ke Huang Lin Yang Egor Dontsov Ding-Wei Weng Hong-Bo Liang Zi-Rui Yin Ji-Zhou Tang | 2022 | Petroleum Science2022，19，1： | 4 |
| 13 | Deep origin of Cenozoic volcanoes in Northeast China revealed by 3-D electrical structure显示文摘The three-dimensional(3-D)electrical structure of the upper-mantle was used to examine the deep origins of and relationship among the Cenozoic volcanoes located in Northeast China(NEC).High-quality,long-period magnetotelluric(LMT)full-impedance tensor data were collected in NEC and subjected to 3-D Gauss-Newton inversion in order to construct a resistivity model.The resulting model reveals the presence of multiple localized low-resistivity anomalies(LRAs)within the high resistivity lithosphere beneath NEC.These LRAs partially coincide with Cenozoic volcanoes on the surface.Three LRAs that form a larger,annular LRA were observed in the deep upper mantle beneath the Songliao Basin,whereas vein-like LRAs were found in the asthenosphere that connect the lithosphere and deep upper mantle.Petrophysical analyses suggest that the LRAs may have been caused by fluid-induced melting.Based on our electrical model,we propose that,following dehydration of the subducted Western Pacific slab into the mantle transition zone(MTZ)beneath NEC,the released water migrated upward and caused partial melting at the top of the MTZ beneath the Songliao Basin.Under the effect of buoyancy,the melted mantle formed a thermal upwelling that caused melting of asthenosphere before diapiring at the base of the dry lithosphere.The magma then penetrated structural boundaries(such as thinner,weaker,or activated suture zones)and finally reached the Earth's surface.This melting and upwelling of hot mantle materials may have resulted in large-scale volcanism in the region throughout the Cenozoic,including the eruption of Changbai Mountain and Halaha Volcanoes.Our results suggest that the Cenozoic NEC volcanoes may all share a similar mode of genesis,and probably originated from the annular LRA in the deep upper mantle. | Shiwen LI Aihua WENG Jianping LI Xuanlong SHAN Jiangtao HAN Yu TANG Yanhui ZHANG Xueqiu WANG | 2020 | Science China Earth Sciences2020，63，4： | 4 |
| 14 | Scalable and ultrafast epitaxial growth of single-crystal graphene wafers for electrically tunable liquid-crystal microlens arrays显示文摘The scalable growth of wafer-sized single-crystal graphene in an energy-efficient manner and compatible with wafer process is critical for the killer applications of graphene in high-performance electronics and optoelectronics. Here, ultrafast epitaxial growth of single-crystal graphene wafers is realized on singlecrystal Cu90Ni10(1 1 1) thin films fabricated by a tailored two-step magnetron sputtering and recrystallization process. The minor nickel(Ni) content greatly enhances the catalytic activity of Cu, rendering the growth of a 4 in. single-crystal monolayer graphene wafer in 10 min on Cu90Ni10(1 1 1), 50 folds faster than graphene growth on Cu(1 1 1). Through the carbon isotope labeling experiments, graphene growth on Cu90Ni10(1 1 1) is proved to be exclusively surface-reaction dominated, which is ascribed to the Cu surface enrichment in the Cu Ni alloy, as indicated by element in-depth profile. One of the best benefits of our protocol is the compatibility with wafer process and excellent scalability. A pilot-scale chemical vapor deposition(CVD) system is designed and built for the mass production of single-crystal graphene wafers, with productivity of 25 pieces in one process cycle. Furthermore, we demonstrate the application of single-crystal graphene in electrically controlled liquid-crystal microlens arrays(LCMLA), which exhibit highly tunable focal lengths near 2 mm under small driving voltages. By integration of the graphene based LCMLA and a CMOS sensor, a prototype camera is proposed that is available for simultaneous light-field and light intensity imaging. The single-crystal graphene wafers could hold great promising for highperformance electronics and optoelectronics that are compatible with wafer process. | Bing Deng Zhaowei Xin Ruiwen Xue Shishu Zhang Xiaozhi Xu Jing Gao Jilin Tang Yue Qi Yani Wang Yan Zhao Luzhao Sun Huihui Wang Kaihui Liu Mark H. Rummeli Lu-Tao Weng Zhengtang Luo Lianming Tong Xinyu Zhang Changsheng Xie Zhongfan Liu Hailin Peng | 2019 | Science Bulletin2019，64，10： | 4 |
| 15 | Treating chronic hepatitis B virus:Chinese physicians' awareness of the 2010 guidelines显示文摘AIM: To investigate Chinese physicians' awareness of the 2010 guidelines on the treatment of chronic hepatitis B virus(HBV) infection.METHODS: This was a quantitative survey that investigated the characteristics and practices of physicians who were treating patients with hepatitis B, the profile of their patients and physician practices regarding the diagnosis and treatment of HBV at the time of the survey. Participants were randomly selected from available databases of Chinese physicians and requested to complete either an online or paper-based survey. Data from the survey responses were analysed. For data validation and interpretation, qualitative in-depth interviews were conducted with 39 of the respondents.RESULTS: Five-hundred completed surveys, from 663 physicians were available for analysis. A mean of 175 chronic hepatitis B(CHB) patients was seen by each physician every month, of whom 85(49%) were treated in line with therapeutic indications stated in the 2010 guidelines. A total of 444(89%) physicians often(> 60% of the time) adhered to the guidelines. Most physicians used antiviral medications as recommended. For patients with compensated and decompensated cirrhosis, 342(68%) and 336(67%) of physicians, respectively, often followed the recommendation to use potent nucleos(t)ide analogues with a high genetic barrier to resistance, using the appropriate treatment more than 60% of the time. Physicians from infectious disease or liver disease departments were better informed than those from gastrointestinal or other departments.CONCLUSION: The majority of Chinese physicians often adhere to Chinese 2010 CHB guidelines and are well-informed about the use of antiviral medications for hepatitis B. | Lai Wei Ji-Dong Jia Xin-Hua Weng Xiao-Guang Dou Jia-Ji Jiang Hong Tang Qin Ning Qing-Qing Dai Run-Qin Li Jie Liu | 2016 | World Journal of Hepatology2016，8，18： | 4 |
| 16 | The first power generation test of hot dry rock resources exploration and production demonstration project in the Gonghe Basin,Qinghai Province,China显示文摘Hot dry rock(HDR)is a kind of clean energy with significant potential.Since the 1970s,the United States,Japan,France,Australia,and other countries have attempted to conduct several HDR development research projects to extract thermal energy by breaking through key technologies.However,up to now,the development of HDR is still in the research,development,and demonstration stage.An HDR exploration borehole(with 236℃ at a depth of 3705 m)was drilled into Triassic granite in the Gonghe Basin in northwest China in 2017.Subsequently,China Geological Survey(CGS)launched the HDR resources exploration and production demonstration project in 2019.After three years of efforts,a sequence of significant technological breakthroughs have been made,including the genetic model of deep heat sources,directional drilling and well completion in high-temperature hard rock,large-scale reservoir stimulation,reservoir characterization,and productivity evaluation,reservoir connectivity and flow circulation,efficient thermoelectric conversion,monitoring,and geological risk assessment,etc.Then the whole-process technological system for HDR exploration and production has been preliminarily established accordingly.The first power generation test was completed in November 2021.The results of this project will provide scientific support for HDR development and utilization in the future. | Er-yong Zhang Dong-guang Wen Gui-ling Wang Wei-de Yan Wen-shi Wang Cheng-ming Ye Xu-feng Li Huang Wang Xian-chun Tang Wei Weng Kuan Li Chong-yuan Zhang Ming-xing Liang Hong-bao Luo Han-yue Hu Wei Zhang Sen-qi Zhang Xian-peng Jin Hai-dong Wu Lin-you Zhang Qing-da Feng Jing-yu Xie Dan Wang Yun-chao He Yue-wei Wang Zu-bin Chen Zheng-pu Cheng Wei-feng Luo Yi Yang Hao Zhang En-lai Zha Yu-lie Gong Yu Zheng Chang-sheng Jiang Sheng-sheng Zhang Xue Niu Hui Zhang Li-sha Hu Gui-lin Zhu Wen-hao Xu Zhao-xuan Niu Li Yang | 2022 | China Geology2022，5，3： | 4 |
| 17 | Control Effect of Bacillus cereus CGMCC4348 against Botrytis cinerea and Its Mechanism显示文摘[Objective]The paper was to study antifungal activity and control mechanism of Bacillus cereus CGMCC4348 against Botrytis cinerea,and further find new methods against the disease.[Method]By quantitative bioassay,in vitro experiment and pot test,the antagonistic protein was extracted with ammonium sulfate precipitation fractionation to determine the control efficiency.EC50of B.cereus CGMCC4348 was 6.19 mg/L.Average controlling effect of B.cereus CGMCC4348 on B.cinerea was 75.8%,equal to the control group which was treated by 50 mg/L pyrimethanil.Bacillus cereus CGMCC4348 had good inhibitory effect against B.cinerea.[Conclusion]The paper provides scientific basis for green control technology against solanaceous vegetable diseases. | Liang Mengmeng Hu Yonghong Yin Jingjing Yang Wenge Tang Rongrong Zhang Yong Liu Youzhou | 2013 | Plant Diseases and Pests2013，4，5： | 3 |
| 18 | Prediction of Mechanical Properties of Hot Rolled Strip by Using Semi-Parametric Single-Index Model显示文摘A semi-parametric single-index model based approach was proposed for prediction of mechanical properties of hot rolled strip. Based on industrial production data, a semi-parametric single-index model was developed by choosing the appropriate kernel function and window width to predict the yield strength, tensile strength and elongation. When data samples are limited, compared with regression method and neural network method, the prediction results show that the semi-parametric single-index model based method is more adaptive and the prediction performance is superior to those by both regression and neural network methods. | ZHAO Yong-hong WENG Yang PENG Ning-qi TANG Guang-bo LIU Zheng-dong | 2013 | Journal of Iron and Steel Research(International)2013，20，7： | 3 |
| 19 | Generation and characterization of stable pig pregastrulation epiblast stem cell lines显示文摘Pig epiblast-derived pluripotent stem cells are considered to have great potential and broad prospects for human therapeutic model developme nt and livestock breeding.Despite on going attempts since the 1990s,no stably defined pig epiblast-derived stem cell line has bee n established.Here,guided by in sights from a large-scale sin gle-cell tran scriptome an alysis of pig embryos from embryonic day(E)0 to E14,specifically,the tracing of pluripotency changes during epiblast development,we developed an in vitro culture medium for establishing and maintaining stable pluripotent stem cell lines from pig E10 pregastrulation epiblasts(pgEpiSCs).Enabled by chemical inhibition of WNT-related signaling in combination with growth factors in the FGF/ERK,JAK/STAT3,and Activin/Nodal pathways,pgEpiSCs maintain their pluripotency transcriptome features,similar to those of E10 epiblast cells,and normal karyotypes after more than 240 passages and have the potential to differentiate into three germ layers.Strikingly,ultradeep in situ Hi-C analysis revealed functional impacts of chromatin 3D-spatial associations on the transcriptional regulation of pluripote ncy marker genes in pgEpiSCs.I n practice,we con firmed that pgEpiSCs readily tolerate at least three rounds of successive gene editi ng and gene rated cloned gen e-edited live piglets.Our findings deliver on the long-a nticipated promise of pig pluripote nt stem cells and open new avenues for biological research,animal husbandry,and regenerative biomedicine. | Minglei Zhi Jinying Zhang Qianzi Tang Dawei Yu Shuai Gao Dengfeng Gao Pengliang Liu Jianxiong Guo Tang Hai Jie Gao Suying Cao Zimo Zhao Chongyang Li Xiaogang Weng Mengnan He Tianzhi Chen Yingjie Wang Keren Long Deling Jiao Guanglei Li Jiaman Zhang Yan Liu Yu Lin Daxin Pang Qianqian Zhu Naixin Chen Jingjing Huang Xinze Chen Yixuan Yao Jingcang Yang Zicong Xie Xianya Huang Mengxin Liu Ran Zhang Qiuyan Li Yiliang Miao Jianhui Tian Xingxu Huang Hongsheng Ouyang Bofeng Liu Wei Xie Qi Zhou Zhonghua Liu Caihong Zheng Mingzhou Li Jianyong Han | 2022 | Cell Research2022，32，4： | 3 |
| 20 | Comprehensive Proteomic Profiling of Patients'Tears Identifies Potential Biomarkers for the Traumatic Vegetative State显示文摘The vegetative state is a complex condition with unclear mechanisms and limited diagnostic, prognostic,and therapeutic methods. In this study, we aimed to explore the proteomic profile of tears from patients in a traumatic vegetative state and identify potential diagnostic markers using tears—a body fluid that can be collected noninvasively. Using i TRAQ quantitative proteomic technology, in the discovery phase, tear samples collected from 16 patients in a traumatic vegetative state and 16 normal individuals were analyzed. Among 1080 identified tear proteins, 57 were upregulated and 15 were downregulated in the patients compared to the controls. Bioinformatics analysis revealed that the differentially-expressed proteins were mainly involved in the wound response and immune response signaling pathways. Furthermore, we verified the levels of 7 differentially-expressed proteins in tears from50 traumatic vegetative state patients and 50 normal controls(including the samples used in the discovery phase) using ELISA. The results showed that this 7-protein panel had a high discrimination ability for traumatic vegetative state(area under the curve = 0.999). In summary, the altered tear proteomic profile identified in this study provides a basis for potential tear protein markers for diagnosis and prognosis of the traumatic vegetative state and also provides novel insights into the mechanisms of traumatic vegetative state. | Qilin Tang Chao Zhang Xiang Wu Wenbin Duan Weiji Weng Junfeng Feng Qing Mao Shubin Chen Jiyao Jiang Guoyi Gao | 2018 | Neuroscience Bulletin2018，34，4： | 3 |
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