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34篇 您的检索式:作者名="Weiwei Wan"
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1The Reference Genome of Tea Plant and Resequencing of 81 Diverse Accessions Provide Insights into Its Genome Evolution and Adaptation显示文摘Tea plant is an important economic crop,which is used to produce the world's oldest and most widely consumed tea beverages.Here,we present a high-quality reference genome assembly of the tea plant(Camellia sinensis var.sinensis)consisting of 15 pseudo-chromosomes.LTR retrotransposons(LTR-RTs)account for 70.38%of the genome,and we present evidence that LTR-RTS play critical roles in genome size expansion and the transcriptional diversification of tea plant genes through preferential insertion in promoter regions and introns.Genes,particularly those coding for terpene biosynthesis pro-teins,associated with tea aroma and stress resistance were significantly amplified through recent tandem duplications and exist as gene clusters in tea plant genome.Phylogenetic analysis of the sequences of 81 tea plant accessions with diverse origins revealed three well-differentiated tea plant populations,support-ing the proposition for the southwest origin of the Chinese cultivated tea plant and its later spread to western Asia through introduction.Domestication and modern breeding left significant signatures on hundreds of genes in the tea plant genome,particularly those associated with tea quality and stress resis-tance.The genomic sequences of the reported reference and resequenced tea plant accessions provide valuable resources for future functional genomics study and molecular breeding of improved cul-tivars of tea plants.Enhua Xia Wei Tong Yan Hou Yanlin An Linbo Chen Qiong Wu Yunlong Liu Jie Yu Fangdong Li Ruopei Li Penghui Li Huijuan Zhao Ruoheng Ge Jin Huang Ali Inayat Mallano Yanrui Zhang Shengrui Liu Weiwei Deng Chuankui Song Zhaoliang Zhang Jian Zhao Shu Wei Zhengzhu Zhang Tao Xia Chaoling Wei Xiaochun Wan 2020Molecular Plant2020,13,7:51
2Unruptured pregnancy in a noncommunicating rudimentary horn at37 weeks with a live fetus:a case report显示文摘We report an extremely rare case of an unruptured non-communicating rudimentary horn full-term pregnancy.A woman who had a uterine malformation was misdiagnosed as uterus didelphys and gave birth to a live,healthy fetus.The correct diagnosis was not made until cesarean section at 37 4/7 weeks.The case suggests that women who are pregnant in a rudimentary horn could obtain a full-term delivery and give birth to a live and healthy baby.Chen Cheng Weiwei Tang Lei Zhang Mei Luo Meihua Huang Xiuling Wu Guiping Wan 2015The Journal of Biomedical Research2015,29,1:11
3Normal Structure and Function of Endothecium Chloroplasts Maintained by ZmMs33-Mediated Lipid Biosynthesis in Tapetal Cells Are Critical for Anther Development in Maize显示文摘Genic male sterility(GMS)is critical for heterosis utilization and hybrid seed production.Although GMS mutants and genes have been studied extensively in plants,it has remained unclear whether chloroplast-associated photosynthetic and metabolic activities are involved in the regulation of anther development.In this study,we characterized the function of ZmMs33/ZmGPAT6,which encodes a member of the glycerol-3-phosphate acyltransferase(GPAT)family that catalyzes the first step of the glycerolipid synthetic pathway.We found that normal structure and function of endothecium(En)chloroplasts maintained by ZmMs33-mediated lipid biosynthesis in tapetal cells are crucial for maize anther development.ZmMs33 is expressed mainly in the tapetum at early anther developmental stages and critical for cell proliferation and expansion at late stages.Chloroplasts in En cells of wild-type anthers function as starch storage sites before stage 10 but as photosynthetic factories since stage 10 to enable starch metabolism and carbohydrate supply.Loss of ZmMs33 function inhibits the biosynthesis of glycolipids and phospholipids,which are major components of En chloroplast membranes,and disrupts the development and function of En chloroplasts,resulting in the formation of abnormal En chloroplasts containing numerous starch granules.Further analyses reveal that starch synthesis during the day and starch degradation at night are greatly suppressed in the mutant anthers,leading to carbon starvation and low energy status,as evidenced by low trehalose-6-phosphate content and a reduced ATP/AMP ratio.The energy sensor and inducer of autophagy,SnRK1,was activated to induce early and excessive autophagy,premature PCD,and metabolic reprogramming in tapetal cells,finally arresting the elongation and development of mutant anthers.Taken together,our results not only show that ZmMs33 is required for normal structure and function of En chloroplasts but also reveal that starch metabolism and photosynthetic activities of En chloroplasts at different developmental stages are essential for normal anther development.These findings provide novel insights for understanding how lipid biosynthesis in the tapetum,the structure and function of En chloroplasts,and energy and substance metabolism are coordinated to maintain maize anther development.Taotao Zhu Ziwen Li Xueli An Yan Long Xiaofeng Xue Ke Xie Biao Ma Danfeng Zhang Yijian Guan Canfang Niu Zhenying Dong Quancan Hou Lina Zhao Suowei Wu Jinping Li Weiwei Jin Xiangyuan Wan 2020Molecular Plant2020,13,11:9
4Allele-defined genome reveals biallelic differentiation during cassava evolution显示文摘Dear Editor,Generation of heterozygous genomes by hybridization between or within species can help maintain plant diversity and serve as a potential source of new species(Baek et al.,2018).Moreover,genomic heterozygosity is associated with genomic coadaptation,developmental stability,and heterosis.Accurate definition of alleles in haplotypes is necessary to precisely characterize allelic variation controlling agriculturally important traits(Shi et al.,2019).Currently,most released genomes have mosaic assembly of haplotypes due to random selection or collapse of alleles during genome assembly(Shi et al.,2019),which masked allelic variation and functional differentiation of divergent alleles in heterozygous species.Wei Hu Changmian Ji Haitao shi Zhe Liang Zehong Ding Jianqiu Ye Wenjun Ou Gang Zhou Weiwei Tie Yan Yan Jinghao Yang Xiaoying Yang Yunxie Wei Zhiqiang Jin Jianghui Xie Ming Peng Wenquan Wang Anping Guo Biyu Xu Jianchun Guo Songbi Chen Liming Ma Mingcheng Wang Yang Zhou Xiaolong Li Ruoxi Li Siyi Guo Xinhui Xiao Zhongqing Wan Feifei An Jie Zhang Qingyun Leng Yin Li Ray Ming Kaimian Li 2021Molecular Plant2021,14,6:8
5Recent progress in organic solar cells(PartⅠmaterial science)显示文摘During past several years,the photovoltaic performances of organic solar cells(OSCs)have achieved rapid progress with power conversion efficiencies(PCEs)over 18%,demonstrating a great practical application prospect.The development of material science including conjugated polymer donors,oligomer-like organic molecule donors,fused and nonfused ring acceptors,polymer acceptors,single-component organic solar cells and water/alcohol soluble interface materials are the key research topics in OSC field.Herein,the recent progress of these aspects is systematically summarized.Meanwhile,the current problems and future development are also discussed.Yahui Liu Bowen Liu Chang-Qi Ma Fei Huang Guitao Feng Hongzheng Chen Jianhui Hou Lingpeng Yan Qingya Wei Qun Luo Qinye Bao Wei Ma Wei Liu Weiwei Li Xiangjian Wan Xiaotian Hu Yanchun Han Yaowen Li Yinhua Zhou Yingping Zou Yiwang Chen Yongfang Li Yongsheng Chen Zheng Tang Zhicheng Hu Zhi-Guo Zhang Zhishan Bo 2022Science China Chemistry2022,65,2:5
6Study of rare earth element effect on microstructures and mechanical properties of an Al-Cu-Mg-Si cast alloy显示文摘The improvements of microstructures and properties of a high strength aluminum cast alloy were studied. The effects of rare earth elements on the microstructures and mechanical properties of the high strength cast alloy Al-Cu-Mg-Si were investigated. The result shows that the addition of rare earth elements can change the microstructures in refining the grain size of the alloy and making the needle-like and laminar eutectic Si to a granular Si. With the increase of the rare earth, the tensile strength and elongation of the alloy increase first and then fall down. The mechanical properties of the alloy will reach the highest value when the content of rare earth elements is about 0.7%.WAN Weiwei HAN Jianmin LI Weijing WANG Jinhua 2006Rare Metals2006,25,z2:5
7基于磁流变液传动的柔顺关节研究:设计,仿真和实验(英文)显示文摘为了实现机器人的内在主动柔顺,应用磁流变液的可控特性,设计了基于磁流变液传动的多盘式柔顺关节。基于Bingham塑性模型建立了柔顺关节的扭矩传递模型,仿真分析了盘片直径、盘片间隙、励磁电流等因素对输出扭矩的影响规律,根据理论分析和仿真,得到柔顺关节的机械结构参数。设计实验平台对柔顺关节样机进行了盘片壁面形貌对动力传递性能影响、空转力矩特性、动力传动特性、静态特性、转速-输出转矩特性以及阶跃输入、阶跃负载情况下的动态响应实验研究。结果表明基于磁流变液传动的柔顺关节盘片表面粗糙度越大,所能传递的扭矩越大,磁流变传动输出稳定,且可以无级调控。蔡世波 韦威 Wan Weiwei 鲍官军 胥芳 2019仪器仪表学报2019,40,1:5
8Multi-objective optimization design of wheat centralized seed feeding device based on particle swarm optimization (PSO) algorithm显示文摘In order to solve the problem of interaction between multiple evaluation indexes of seed metering performance under multiple factors of centralized seed feeding device,a multi-objective optimization of structure based on particle swarm optimization(PSO)algorithm was proposed in this paper.The wheat centralized seed feeding device was taken as the research object,and the experimental factors were cone angle of type hole,working speed and seed filling gap.The working process of wheat centralized seed feeding device was simulated by discrete element method(DEM).The average seed number of type hole,the variation coefficient of the average seed number of type hole,and the maximum tangential force between seed and seed feeding mechanism were selected as the evaluation indexes.Through the variance analysis of the evaluation indexes by the experimental factors,the optimization objective function was constructed.Using PSO algorithm,the multi-objective optimization was carried out for the wheat centralized seed feeding device.The optimization results show that the best structural combination parameters of the wheat centralized seed feeding device are the hole cone angle of 31.6°and the seed filling gap of 4.6 mm.The validity of the method was verified by simulation and field test.The results show that the PSO algorithm multi-objective optimization method proposed in this paper can provide a reference for the structural improvement and optimal design of the centralized seed feeding device.Qingqing Wang Zhaodong Li Weiwei Wang Chunling Zhang Liqing Chen Ling Wan 2020International Journal of Agricultural and Biological Engineering2020,13,6:4
9Preparation of an anti-diethylstilbestrol monoclonal antibody and development of an indirect competitive ELISA to detect diethylstilbestrol in biological samples显示文摘Based on the preparation of an anti-diethylstilbestrol(DES) monoclonal antibody,a simple and convenient indirect competitive enzyme-linked immunosorbent assay(ELISA) method for DES detection has been developed.The monoclonal antibody demonstrated high sensitivity to DES with an IC50 value of 275 pg mL-1 and detection limit(LOD) of 90 pg mL-1.The specificity of the assay was studied by measuring cross-reactivity of the antibody with structurally related compounds of ethinyl estradiol(<7%),estrone(<0.1%),estriol(<0.1%),and diethylstilbestrol benzoate(<0.1%).Chicken,fish,shrimp,urine and bile spiked with different concentration of DES were detected by the developed method,and the recovery rates were greater than 79.5%.Intra-and inter-assay variations were about 6%.This method exhibited high stability with a coefficient of variation less than 10% in buffer and in real samples.The LODs in fish/shrimp,liver,feed and urine spiked with DES were 600,600,4800 and 600 pg mL-1,respectively.These results confirmed that the antibody to DES was successfully produced and could be used to establish ELISA methods for DES detection in food producing animals.LI WeiHua MENG Meng HE FangYang WAN YuPing XUE HuYin LIU Wei YIN WeiWei XU Jing FENG CaiWei WANG ShanLiang LU Xiao LIU JinTing XI RiMo 2011Chinese Science Bulletin2011,56,8:4
10Survey of optimal motion planning显示文摘Optimal motion planning becomes more and more important these days and is critical for motion planning algorithms developed in the academia to be applicable to real-world applications.The objective of this article is to survey the current state-of-the-art on optimal motion planning algorithms in terms of their three main components:the decision variables and problem formulation,constraints,and objectives.A selection of proposed techniques is reviewed along with a discussion of their effectiveness and limitations.The side-by-side comparison presented in this survey helps to gain insight into the strengths and limitations of the reviewed approaches and assists with system-level design choices.Yajue Yang Jia Pan Weiwei Wan 2019IET Cyber-Systems and Robotics2019,1,1:4
11Urban Plant Diversity in Relation to Land Use Types in Built-up Areas of Beijing显示文摘Urban plants provide various ecosystem services and biodiversity for human well-being. It is necessary to examine the plant species and functional traits composition and the influencing factors. In this study, a field survey was conducted using the tessellation-randomized plot method to assess the plant species and functional traits variability in greenspaces across eight land use types(LUTs) in the built-up areas of Beijing, China. Results showed that the woody plants in the built-up areas of Beijing comprised 85 non-native species(57%), 21 pollen-allergenic species(14%), and 99 resistant species(67%). Residential areas, community parks and institutional areas had higher woody plant species richness than other LUTs. Native and extralimital native species were more widespread than exotic species. Proportions of species with resistances were low except for cold-and drought-resistance; consequently, a high intensity of management and maintenance is essential for survival of plants in this urban area. Caution should be exerted in selecting plant species with resistance to harsh conditions in different LUTs. Housing prices, distances from the urban center, years since the establishment of LUTs and greening rate were strongly correlated with the plant functional traits and species diversity. Urban forest managers should consider plant functional traits and LUT-specific strategies to maximize both forest and human health.GUO Peipei SU Yuebo WAN Wuxing LIU Weiwei ZHANG Hongxing SUN Xu OUYANG Zhiyun WANG Xiaoke 2018Chinese Geographical Science2018,28,1:4
12OsPKpa_1 encodes a plastidic pyruvate kinase that affects starch biosynthesis in the rice endosperm显示文摘Pyruvate kinase(PK) is a key enzyme in glycolysis and carbon metabolism. Here, we isolated a rice(Oryza sativa) mutant, w59, with a white-core floury endosperm. Map-based cloning of w59 identified a mutation in OsPKpa_1, which encodes a plastidic isoform of PK(PKp). OsPKpa_1 localizes to the amyloplast stroma in the developing endosperm, and the mutation of OsPKpa_1 in w59 decreases the plastidic PK activity, resulting in dramatic changes to the lipid biosynthesis in seeds. The w59 grains were also characterized by a marked decrease in starch content. Consistent with a decrease in number and size of the w59 amyloplasts, large empty spaces were observed in the central region of the w59 endosperm, atthe early grain-filling stage. Moreover, a phylogenetic analysis revealed four potential rice isoforms of OsPKp. We validated the in vitro PK activity of these OsPKps through reconstituting active PKp complexes derived from inactive individual OsPKps, revealing the heteromeric structure of rice PKps, which was further confirmed using a protein–protein interaction analysis. These findings suggest a functional connection between lipid and starch synthesis in rice endosperm amyloplasts.Yue Cai Wenwei Zhang Jie Jin Xiaoming Yang Xiaoman You Haigang Yan Liang Wang Jie Chen Jiahuan Xu Weiwei Chen Xingang Chen Jing Ma Xiaojie Tang Fei Kong Xiaopin Zhu Guoxiang Wang Ling Jiang William Terzaghi Chunming Wang Jianmin Wan 2018Journal of Integrative Plant Biology2018,60,11:3
13Recent progress in organic solar cells(PartⅡdevice engineering)显示文摘1 Introduction Organic solar cells(OSCs)belong to a multidisciplinary field composed of chemistry,materials science,physics,engineering,etc.For a better reviewing of this field,we briefly divide the research field of OSCs into two parts:material science and device engineering.In our previous review,the material science part of OSCs,including conjugated polymer donors and acceptors,small molecular donors and acceptors.Yahui Liu Bowen Liu Chang-Qi Ma Fei Huang Guitao Feng Hongzheng Chen Jianhui Hou Lingpeng Yan Qingya Wei Qun Luo Qinye Bao Wei Ma Wei Liu Weiwei Li Xiangjian Wan Xiaotian Hu Yanchun Han Yaowen Li Yinhua Zhou Yingping Zou Yiwang Chen Yuqiang Liu Lei Meng Yongfang Li Yongsheng Chen Zheng Tang Zhicheng Hu Zhi-Guo Zhang Zhishan Bo 2022Science China Chemistry2022,65,8:3
14Lipidomic and transcriptomic analysis reveals reallocation of carbon flux from cuticular wax into plastid membrane lipids in a glossy“Newhall”navel orange mutant显示文摘Both cuticle and membrane lipids play essential roles in quality maintenance and disease resistance in fresh fruits.Many reports have indicated the modification of alternative branch pathways in epicuticular wax mutants;however,the specific alterations concerning lipids have not been clarified thus far.Here,we conducted a comprehensive,timeresolved lipidomic,and transcriptomic analysis on the“Newhall”navel orange(WT)and its glossy mutant(MT)“Gannan No.1”.The results revealed severely suppressed wax formation accompanied by significantly elevated production of 36-carbon plastid lipids with increasing fruit maturation in MT.Transcriptomics analysis further identified a series of key functional enzymes and transcription factors putatively involved in the biosynthesis pathways of wax and membrane lipids.Moreover,the high accumulation of jasmonic acid(JA)in MT was possibly due to the need to maintain plastid lipid homeostasis,as the expression levels of two significantly upregulated lipases(CsDAD1 and CsDALL2)were positively correlated with plastid lipids and characterized to hydrolyze plastid lipids to increase the JA content.Our results will provide new insights into the molecular mechanisms underlying the natural variation of plant lipids to lay a foundation for the quality improvement of citrus fruit.Haoliang Wan Hongbo Liu Jingyu Zhang Yi Lyu Zhuoran Li Yizhong He Xiaoliang Zhang Xiuxin Deng Yariv Brotman Alisdair RFernie Yunjiang Cheng Weiwei Wen 2020Horticulture Research2020,7,1:2
15Medroxyprogestogen enhances apoptosis of SKOV-3 cells via inhibition of the PI3K/Akt signaling pathway显示文摘We sought to assess the effect of progestin on the apoptosis of epithelial ovarian cancer cell line SKOV-3 and via regulation of phosphorylation signaling in.Epithelial ovarian cancer cell line SKOV-3 was treated with medroxyprogestogen,phosphatidylinositol 3-kinase inhibitor LY294002 and vehicle control.Akt,phospho-Akt,Bcl-2 and phospho-Bad proteins were examined by immunoblotting assays.Medroxyprogestogen-induced apoptosis was assessed by MTT assays and Annexin V apoptosis assay.We found no significant difference in Akt and Bad expression in both the medroxyprogestogen groups and the control group.The levels of phospho-Akt,Bcl-2 and phospho-Bad were decreased in all the medroxyprogestogen groups and significantly decreased in the high dose mitogen-activated protein (MAP) group (10 μmol/L).Viability of SKOV-3 was reduced and apparent apoptosis of SKOV-3 cells was observed with increased doses of MAP.The findings suggest that medroxyprogestogen can induce SKOV-3 cell apoptosis by inhibiting Akt phosphorylation.Yan Li Yi Jiang Yicong Wan Lin Zhang Weiwei Tang Jingjing Ma Shan Wu Wenjun Cheng 2013The Journal of Biomedical Research2013,27,1:2
16Determination of HCH and DDT in soils following Wenchuan 5.12 Violent Earthquake in China显示文摘A large number of disinfectants and insecticides were used on a large scale after the Wenchuan 5.12 Violent Earthquake. The disinfectants and insecticides, including HCH (hexachlorocyclohexane) and DDT (dichlorodiphenyltrichoroethane) as representative OCPs, could lead to serious environmental problems. In order to disclose the residues of HCH and DDT in soils following the Wenchuan 5.12 Violent Earthquake, surface soils in Dujiangyan City and Beichuan County were sampled in 2009. The samples were analyzed by GC/MS after pre- treatment by ultrasonic solvent extraction. It has been found that the concentra-tions of γ-HCH, β-HCH and 4,4'-DDT in soils of Beichuan County were 94–102.4, 14.1–36.1 and 2.9–4.3 times high their concentrations in those of the Wolong Nature Reserve, and the concentrations in soils of Dujiangyan City were 52.4–61.6, 8.8 –45.3 and 5.4–6.2 times high their concentrations in soils of the Wolong Nature Reserve, respectively. According to the isomers of HCHs and metabolites of DDTs, it could be concluded that these areas may have additional inputs of lindane and 4,4'-DDT.CUI Weiwei ZHANG Qiangbin ZHU Xianlei WAN Yunyang ZHANG Zhihuan FAN Zhihua WANG Tieguan 2011Chinese Journal Of Geochemistry2011,30,1:2
17Geodynamic processes of the southeastern Neo-Tethys Ocean and the formation mechanism of the curved subduction system in Southeast Asia显示文摘Southeast Asia is located at the intersection of the Tethys and Pacific domains. The superimposed effects of the two tectonic domains have resulted in complicated deep structure, surface magma responses, and dynamic processes of Southeast Asia. Based on the latest long-term passive seismic experiment and numerical modeling, this study reconstructs the dynamic processes of the closure of the Neo-Tethys Ocean and the formation of the curved subduction system in Southeast Asia since the Late Mesozoic. P-wave velocity structure shows a remnant of the Neo-Tethys subducted slab in the lower mantle beneath Southeast Asia at a depth of approximately 1500 km. On the Java-East Timor subduction zone, the remnant slab is coupled with the Indo-Australian subducting slab in the upper mantle with the same direction, while on the Sumatra subduction zone, the remnant slab is decoupled from the Indo-Australian subducting slab in different directions. The formation of the curved subduction system in Southeast Asia is resulted from the northward subdcutions of previous Neo-Tethys and current IndoAustralian Plate, and the westward subduction of the Pacific Plate since Mesozoic. The former is characterized by continuous subduction and subsequent continental block collision, forming the current continental lithosphere in Southeast Asia and the curve-shaped Sumatra-Java subduction zone;the latter is characterized by subduction retreat and back-arc spreading, forming the eastern Philippine subduction zone and a series of marginal sea basins. Since the Early Cretaceous, the opening of the North Australian Sea resulted in stagnation of the Australian Block in the high latitude area of the southern hemisphere for a long time.The North Australian Sea was dominated by out-dipping double subduction from 45 Ma, which resulted in rapid northward drifting of the Australian Block and final collision with the Sundaland.Weiwei DING Rixiang ZHU Bo WAN Liang ZHAO Xiongwei NIU Pan ZHAO Baolu SUN Yanghui ZHAO 2023Science China Earth Sciences2023,66,4:2
18Integrated multi-omics analysis of developing‘Newhall’orange and its glossy mutant provide insights into citrus fragrance formation显示文摘Sesquiterpene valencene is dominant in flavedo tissues of sweet oranges and imparts a unique woody aroma.However,the interaction between the biosynthetic pathways of valencene and other nutritional compounds is less studied.Sesquiterpenoids were significantly accumulated in a previously reported glossy mutant of orange(MT)than the wild type(WT),especially valencene and caryophyllene.In addition,we identified several other pathways with variations at both the transcriptional and metabolic levels in MT.It’s interesting to found those upregulated metabolites in MT,such as eukaryotic lipids,kaempferol and proline also showed strong positive correlation with valencene along with fruit maturation while those down-regulated metabolites,such as phenylpropanoid coumarins and most of the modified flavonoids exhibited negative correlation.We then categorized these shifted pathways into the‘sesquitepenoid-identical shunt’and the sesquitepenoid-opposite shunt’and confirmed the classification result at transcriptional level.Our results provide important insights into the connections between various fruit quality-related properties.Haoliang Wan Xiaoliang Zhang Ping Wang Haiji Qiu Yafei Guo Yunjiang Cheng Weiwei Wen 2022Horticultural Plant Journal2022,8,4:2
19Yin Yang 1 Plays an Essential Role in Breast Cancer and Negatively Regulates p27显示文摘Meimei Wan Weiwei Huang Timothy E. Kute Lance D. Miller Qiang Zhang Heather Hatcher Jingxuan Wang Daniel B. Stovall Gregory B. Russell Paul D. Cao Zhiyong Deng Wei Wang Qingyuan Zhang Ming Lei Suzy V. Torti Steven A. Akman Guangchao Sui 2012The American Journal of Pathology2012,,:1
20MicroRNA-206 overexpression promotes apoptosis, induces cell cycle arrestand inhibits the migration of human hepatocellular carcinoma HepG2 cells显示文摘Weiwei Liu Chuanming Xu Huifang Wan Chunju Liu Can Wen Hongfei Lu Fusheng Wan 2014International Journal of Molecular Medicine2014,,2:1
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