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| 1 | Conservation analysis of long non-coding RNAs in plants显示文摘Long non-coding RNAs(lncRNAs) are gene regulators that have vital roles in development and adaptation to the environment in eukaryotes. However, the structural and evolutionary analyses of plant lncRNAs are limited. In this study, we performed an analysis of lncRNAs in five monocot and five dicot species. Our results showed that plant lncRNA genes were generally shorter and had fewer exons than protein-coding genes. The numbers of lncRNAs were positively correlated with the numbers of protein-coding genes in different plant species, despite a high range of variation. Sequence conservation analysis showed that the majority of lncRNAs had high sequence conservation at the intra-species and sub-species levels, reminiscent of protein-coding genes. At the inter-species level, a subset of lncRNAs were highly diverged at the nucleotide level, but conserved by position.Interestingly, we found that plant lncRNAs have identical splicing signals, and those which can form precursors or targets of miRNAs have a conservative identity in different species. We also revealed that most of the lowly expressed lncRNAs were tissue-specific, while those highly conserved were constitutively transcribed. Meanwhile, we characterized a subset of rice lncRNAs that were co-expressed with their adjacent protein-coding genes, suggesting they may play cis-regulatory roles. These results will contribute to understanding the biological significance and evolution of lncRNAs in plants. | Pingchuan Deng Shu Liu Xiaojun Nie Song Weining Liang Wu | 2018 | Science China(Life Sciences)2018，61，2： | 4 |
| 2 | Study on liquids diffusion into and relevant corrosion behaviour of glass fibre reinforced polymer used in high voltage composite insulator显示文摘Long-term liquids diffusion tests were performed on glass fibre reinforced polymer(GFRP)used in high voltage composite insulator.The plate-shaped GFRP specimens were immersed in three types of liquids,which high voltage composite insulator would encounter in practice,including deionised water,NaCl solution and nitric acid solution for a period of 4000 h.Chemical reaction and physical dissolution complicated the diffusion processes,which could be divided into three types based on the features of diffusion curves.Fourier transform infrared spectroscopy,scanning electron microscopy and optical interferometry were employed to investigate the chemical property,two-dimensional(2D)and 3D surface morphologies of GFRP.The results revealed that the surface layer of GFRP was destroyed to some extent depending on the type and concentration of the liquid,the maximum of profile height deviation of GFRP surface was proposed to evaluate the extent of the corrosion of GFRP,the greater the profile height deviation,the more serious the corrosion of GFRP.The present work is helpful for a better understanding of the diffusion behaviour of liquids into GFRP used in high voltage composite insulator as well as in other industry applications. | Yanfeng Gao Xidong Liang Weining Bao Chao Wu Shaohua Li Yingyan Liu Yuanji Cai | 2020 | High Voltage2020，5，1： | 2 |
| 3 | Purification and Photodynamic Bioactivity of Phycoerythrin and Phycocyanin from Porphyra yezoensis Ueda显示文摘Phycoerythrin and phycocyanin were purified from Porphyra yezoensis Ueda with their bioactivity determined in this study. Continuous precipitation with ammonium sulfate at different concentrations(10%, 20%, 40% and 50%) increased the purity(A564:A280) of phycoerythrin to 1.49, 3.92 fold of the raw extract(0.38) and the purity(A615:A280) of phycocyanin to 0.70, 3.33 fold of the raw extract(0.21). Two more times of chromatography with hydroxylapatites finally made the purity of phycoerythrin and phycocyanin reach 5.50, 14.47 fold of the raw extract, and 5.10, 24.29 fold of the raw extract, respectviely. The yield of high purity phycoerythrin and phycocyanin were 0.21% and 0.09% of dried P. yezoensis blade, respectively. The photodynamic cytotoxic experiment showed that both phycoerythrin and phycocyanin inhibited the growth of liver tumor cells significantly. It was found that 250 mg L-1 purified phycoerythrin and phycocyanin inhibited the growth of hepatocellular carcinoma cells 24 h after laser-irradiation by 80% and 59%, respectively, and 100 mg L-1 purified phycoerythrin and phycocyanin induced the apoptosis of 31.54% and 32.54% of the cells, respectively, 8 h after photodynamic therapy. Oue findings demonstrated that P. yezoensis can serve as photosensitizer(phycoerythrin and phycocyanin) producer. | CAI Chuner WANG Yuan LI Chunxia GUO Ziye JIA Rui WU Weining HU Yan HE Peimin | 2014 | Journal of Ocean University of China2014，13，3： | 2 |
| 4 | Vapor phase growth of two-dimensional PdSe2 nanosheets for high-photoresponsivity near-infrared photodetectors显示文摘Palladium diselenide(PdSe2),a stable layered material with pentagonal structure,has attracted extensive interest due to its excellent electrical and optoelectronic performance.Here,we report a reliable process to synthesize PdSe2 via chemical vapor deposition(CVD)method.Through systematic regulation of temperature in the growth process,we can tune the thickness,size,nucleation density and morphology of PdSe2 nanosheets.Field-effect transistors based on PdSe2 nanosheets exhibit n-type behavior and present a high electron mobility of 105 cm^2·V^−1·s^−1.The electrical property of the devices after 6 months keeping in the air show little change,implying outstanding air-stability of PdSe2.In addition,PdSe2 near-infrared photodetector shows a photoresponsivity of 660 A·W^−1 under 914 nm laser.These performances are better than those of most CVD-grown 2D materials,making ultrathin PdSe2 a highly qualified candidate material for next-generation optoelectronic applications. | Weiting Xu Jiayang Jiang Huifang Ma Zhengwei Zhang Jia Li Bei Zhao Ruixia Wu Xiangdong Yang Hongmei Zhang Bailing Li Weining Shu Zucheng Zhang Bo Li Yuan Liu Lei Liao Xidong Duan | 2020 | Nano Research2020，13，8： | 1 |
| 5 | Collaborative resource sharing between public and school libraries显示文摘 | Smith D Shea M Wu Weining | 2014 | Interlending & Document Supply2014，42，4： | 1 |
| 6 | Urban ecology in China:historical developments and future directions显示文摘 | Wu Jianguo Xiang Weining Zhao Jingzhu | 2014 | Landscape and Urban Planning2014，125，5： | 1 |
| 7 | Effect of sourdough fermentation on the quality of Chinese Northern-style steamed breads显示文摘 | Chao Wu Ruoshi Liu Weining Huang Patricia Rayas-Duarte Feng Wang Yuan Yao | 2012 | Journal of Cereal Science2012，，2： | 1 |
| 8 | Effect of sourdough fermentation on the quality of Chinese Northern - style steamed breads显示文摘 | Chao Wu Ruoshi Liu Weining Huang | 2012 | Journal of Cereal Science2012，，56： | 1 |
| 9 | Analysis of a thress-way race between tumor growth, a rephcation-competent virus and an immune response 显示文摘 | WU J T KIRN D H WEIN L M | 2004 | Bull Math Biol2004，66，： | 1 |
| 10 | Effect of filled-concrete height on ultimate strength and ductility of steel tubular bridge pier with partial filled-concrete 显示文摘 | WANG Zhanfei WU Quan SUI Weining | 2011 | Advanced materials research2011，，803806： | 1 |
| 11 | Synthesis and fluorescence of Tb3+ and Eu3+ complexes with N,N',N',-1,3,5-triazine-2,4,6-triyltris--glycine显示文摘 | Meng Jianxin Wu Weining | 1995 | Journal of Rare Earth1995，13，3： | 1 |
| 12 | High Voltage Digital Transient State Measuring System显示文摘This paper presents main functions of a high voltage,high speed and extensive memory transient measurementequipment and design of associate hardware and software. Thepaper also introduces the hardware design of high speed hugememory data sampler, explains the know-how of data analysissoftware, program-controlled voltage divider andanti-interference measurement, with which the equipmentperforms excellent on--site.This equipment reallies automatic measurement and satisfiesthe requirement of high voltage digital measurment. | Wu Weining Chang Wenliang Xu Yong Li Jianjian Mei Cang RongJiangang(Wuhan Research Institute of High Voltage Technology, State Power Corporation)Yao Zonggan(Central China University of Technology)Xie Jing(Fujian Electric Power Testing & Research Insti | 1999 | Electricity1999，，3： | 0 |
| 13 | High-Speed Numeral Filter in Impulse Voltage Measurement显示文摘Since the CPU of embed system has some limitation in operating speed, a new filter was put forward which implemented mountain template convolution by performing rectangle template convolution two times. It can obtain time and frequency localization with computational complexity greatly reduced. This algorithm was applied to lightning waveforms (include chopped waveforms) parameter calculation. It simplifies the computation and the results pretreated by this algorithm are in accord with IEC1083-2 completely. It was applied in embed system successfully. Its capability in frequency restraining was researched. The validity of the algorithm was proved in theory when processing lightning waves. The standard sources and the processing results are consistent completely. | Zheng Xiaodong Wu Weining Huang Xinghan Wang Min | 2005 | Electricity2005，16，3： | 0 |
| 14 | Effects of liquids immersion and drying on the surface properties of HTV silicone rubber:characterisation by contact angle and surface physical morphology显示文摘High-temperature vulcanised(HTV)silicone rubber composite insulator has been widely used in extra-high and ultra-high transmission lines due to its excellent electrical and mechanical performance.In practice use,liquids may encounter the composite insulator intermittently and can lead to the degradation and deterioration of silicone rubber.In the present research,the effects of liquids immersion and subsequent drying on the surface properties of HTV silicone rubber were investigated by means of the contact angle measurement and surface physical morphology measurement which was further analysed in terms of the discrete wavelet transform method,multiresolution signal decomposition algorithm,and three-dimensional fractal dimension calculation.Based on the experimental results,the mechanism of the loss and recovery of the hydrophobicity of HTV silicone rubber surface during the liquids immersion and subsequent drying processes were further explained by taking into consideration the embedded water molecules(and/or hydrated ions)on the HTV silicone rubber surface and the synergy of the increase in roughness/fractal dimension and low molecular weight siloxanes diffusion.This study is helpful for better understanding of the change of surface properties of HTV silicone rubber caused by environmental and electrical stresses. | Yanfeng Gao Xidong Liang Weining Bao Shaohua Li Chao Wu Yingyan Liu Yuanji Cai | 2019 | High Voltage2019，4，1： | 0 |
| 15 | Failure analysis of a field brittle fracture composite insulator:characterisation by Xray photoelectron spectroscopy analysis显示文摘In the present work,a field brittle fracture composite insulator was collected and examined with the purpose of obtaining a better understanding of the failure features.Firstly,the macroscopic and microscopic fractography of this fractured composite insulator was observed confirming that the classical brittle fracture morphology features appeared in this field failed composite insulator.Secondly,the X-ray photoelectron spectroscopy(XPS)analysis was performed on the fracture surface of the glass-fibre-reinforced polymer rod.By examining the XPS survey spectra and high-resolution XPS spectra for nitrogen,sulphur,silicon,calcium,aluminium,boron and carbon elements,the type of the glass fibre and the acid source responsible for this brittle fracture composite insulator were confirmed.Furthermore,the oxidation of the silicate network of glass fibre at the fracture surface was first observed,meanwhile,the oxidation of the epoxy resin matrix was also observed.The leaching of non-silicate constituents leading to a high-silica glass fibre was also observed;moreover,in comparison with the calcium and aluminium elements,the boron element resides in the silicate network was more preferential to be leached.The present work provides an insight into the brittle fracture in high voltage composite insulator from chemical properties point of view. | Yanfeng Gao Xidong Liang Weining Bao Chao Wu Shaohua Li | 2019 | High Voltage2019，4，2： | 0 |
| 16 | Traceless photodegradable polymer cocoons for universal protein delivery and light-controlled gene editing显示文摘Polymer conjugation was found highly valuable in clinic to improve the bioavailability of protein therapeutics.However,it is still a tremendous challenge to achieve a complete release of original proteins from the conjugated hybrid under external stimulus to recover active proteins in the targeted tissue.Herein,we report a general light-controlled protein delivery methodology by weaving a photodegradable polymer cocoon around proteins,which could reliably protect them from degradation in the dark while efficiently releasing them under illumination without any residual atoms.The surface charge of the polymer shell is easily tunable to facilitate efficient cell uptake.The versatility of this strategy is demonstrated by the delivery of the Cas9/sg RNA complex that realized light-controlled gene editing both in vitro and in vivo,and such repertoire is of particular value in regard to minimizing the off-target toxicity of CRISPR-Cas9-based gene therapy. | Huaibin Yu Meron Tsegay Kifle Lili Chen Feihong Xiong Yan Zhang Shangbang Gao Wei Hou Shuliang Chen Weining Zhao Yuzhou Wu | 2024 | Science China Chemistry2024，67，2： | 0 |
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