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| 1 | Cryopreservation for delayed circulating tumor cell isolation is a valid strategy for prognostic association of circulating tumor cells in gastroesophageal cancer显示文摘AIM To demonstrate the feasibility of cryopreservation of peripheral blood mononuclear cells(PBMCs) for prognostic circulating tumor cell(CTC) detection in gastroesophageal cancer.METHODS Using 7.5 m L blood samples collected in EDTA tubes from patients with gastroesopheagal adenocarcinoma, CTCs were isolated by epithelial cell adhesion molecule based immunomagnetic capture using the Iso Flux platform. Paired specimens taken during the same blood draw(n = 15) were used to compare number of CTCs isolated from fresh and cryopreserved PBMCs. Blood samples were processed within 24 h to recover the PBMC fraction, with PBMCs used for fresh analysis immediately processed for CTC isolation. Cryopreservation of PBMCs lasted from 2 wk to 25.2 mo(median 14.6 mo). CTCs isolated from pre-treatment cryopreserved PBMCs(n = 43) were examined for associations with clinicopathological variables and survival outcomes.RESULTS While there was a significant trend to a decrease in CTC numbers associated with cryopreserved specimens(mean number of CTCs 34.4 vs 51.5, P = 0.04), this was predominately in samples with a total CTC count of > 50, with low CTC count samples less affected(P = 0.06). There was no significant association between the duration of cryopreservation and number of CTCs. In cryopreserved PBMCs from patient samples prior to treatment, a high CTC count(> 17) was associated with poorer overall survival(OS)(n = 43, HR = 4.4, 95%CI: 1.7-11.7, P = 0.0013). In multivariate analysis, after controlling for sex, age, stage, ECOG performance status, and primary tumor location, a high CTC count remained significantly associated with a poorer OS(HR = 3.7, 95%CI: 1.2-12.4, P = 0.03). CONCLUSION PBMC cryopreservation for delayed CTC isolation is a valid strategy to assist with sample collection, transporting and processing. | Daniel Brungs David Lynch Alison WS Luk Elahe Minaei Marie Ranson Morteza Aghmesheh Kara L Vine Martin Carolan Mouhannad Jaber Paul de Souza Therese M Becker | 2018 | World Journal of Gastroenterology2018，24，7： | 5 |
| 2 | Comparison of some French-American hybrid wines with white Riesling using gas chromatography-olfactometry显示文摘 | CHISHOLM MARY G GUIHER L ElSE A VONAH THERESE M | 1994 | The American Journal of Enology and Viticulture1994，45，2： | 1 |
| 3 | Fabrication of a silica coating on magnetic γ-Fe2O3 nanoparticles by an immobilized enzyme 显示文摘 | Shukoor M I Natalio F Therese H A | 2008 | Chem Mater2008，20，： | 1 |
| 4 | The basis for activity controlled rate variable cardiac pacemakers:an analysis of mechanical forces on the human body induced by exercise and environment显示文摘 | Alt E Matula M Theres H | | 0，，10： | 1 |
| 5 | From single mole- cules to nanoscopically structured functional materials: Au nanocrystal owth on TiO2 nanowires controlled by surface bound silicatein 显示文摘 | TAH]R M N EBERHARDT M THERESE H A | | An- gew Chem Iut Ed 2X060，45，29： | 1 |
| 6 | Isoperimetric monotonicity and isoperimetric inequalities of Payne- Rayner type for the first eigenfunction of the Helmholtz problem 显示文摘 | Therese M Jobin K | 1981 | Zeitschrift fiir Angewandte Mathematik und Physik1981，32，6： | 1 |
| 7 | Corticotropin-releasing hormone effects on human pregnant vs nonpregnant myometrium explants estimated from a mathematical model of uterine contraction显示文摘 | Therese M M Brigitte P Bruno C | 2005 | J Appl Physiol2005，99，： | 1 |
| 8 | The contribution of publicly provided inputs to states ' economies 显示文摘 | Teresa G Therese J M | 1992 | Regional Science and Urban Economics1992，22，2： | 1 |
| 9 | Epidural butorphanol or morphine for the relief of post-cesarean section pain:ventilatnry responses to carbon dioxide 显示文摘 | Therese KA Moore M Zhu J | 1987 | Anesth Analg1987，66，： | 1 |
| 10 | Surface-enhance Raman spectroscopy as an Anacillory High-performance Liquid Chromatography Detector for Nitro-phenol Compounds 显示文摘 | Fan N I Lana T Therese M S | 1989 | Anal Chem1989，61，8： | 1 |
| 11 | A New Concept for the Role of Ex vivo Sentinel Lymph Nodes in Node-Negative Colorectal Cancer显示文摘 | Bruno M?rkl Hans M. Arnholdt Hendrik J?hnig Hanno Spatz Matthias Anthuber Daniel V. Oruzio Therese Gannon Kerwel | 2010 | Annals of Surgical Oncology2010，，10： | 1 |
| 12 | Effect of cutaneous stimulation on pain reduction in Emergency Department patients显示文摘 | Sylvia M Kubsch Therese Neveau Kathryn Vandertie | 2000 | Complementary Therapies in Nursing and Midwifery2000，，1： | 1 |
| 13 | Evaluation ofPCR based DNA sequencing targeting mitochondrial larger sub-unit (mtLSU) region for the rapid detection of Pneumocystis ji-rovecii in sputum from HIV positive patient's 显示文摘 | Revathy M Therese KL Chandrasekar C | 2012 | BMC InfectDis2012，12，1： | 1 |
| 14 | Proportioning high-strength concrete to control creep and shrinkage 显示文摘 | COLLINS THERESE M | 1989 | ACI Mater J1989，86，6： | 1 |
| 15 | Evapotrans- piration and seed yield of field grown soybean under deficit irrigation conditions 显示文摘 | FADI K RANDA M THERESE SFEIR | 2005 | Agricultural Water Management2005，75，： | 1 |
| 16 | Evaluation ofPCR based DNA sequencing targeting mitochondrial larger subunit(mtLSU)region for the rapid detection of Pneumocystis jirovecii insputum from HIV positive patients显示文摘 | Revathy M Therese K L Chandrasekar C | 2012 | BMC Infect Dis2012，12，： | 1 |
| 17 | Electrochemical synthesis of perovskite oxides 显示文摘 | Therese G H A Dinamani M Kamath P V | 2005 | Journal of Applied Electrochemistry2005，35，5： | 1 |
| 18 | Anaerobic granular sludge bioreactor technology显示文摘 | Sharon M H Caroline O R Therese M | | 0，，2： | 1 |
| 19 | Endothelin A receptors in human uterine leiomyomas显示文摘 | Breuiller-Fouche M Marie-Cecile MD Therese F | 1997 | Obst Gynecol1997，90，5： | 1 |
| 20 | The discriminatory power of MALDI-TOF mass spectrometry to differentiate between isogenic teicoplanin-susceptible and teicoplanin-resistant strains of methicillin-resistant Staphylococcus aureus 显示文摘 | PAUL A M THERESE M | 2006 | J Mass Spectrometry2006，225，3： | 1 |
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