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| 1 | Cryopreservation for delayed circulating tumor cell isolation is a valid strategy for prognostic association of circulating tumor cells in gastroesophageal cancer显示文摘AIM To demonstrate the feasibility of cryopreservation of peripheral blood mononuclear cells(PBMCs) for prognostic circulating tumor cell(CTC) detection in gastroesophageal cancer.METHODS Using 7.5 m L blood samples collected in EDTA tubes from patients with gastroesopheagal adenocarcinoma, CTCs were isolated by epithelial cell adhesion molecule based immunomagnetic capture using the Iso Flux platform. Paired specimens taken during the same blood draw(n = 15) were used to compare number of CTCs isolated from fresh and cryopreserved PBMCs. Blood samples were processed within 24 h to recover the PBMC fraction, with PBMCs used for fresh analysis immediately processed for CTC isolation. Cryopreservation of PBMCs lasted from 2 wk to 25.2 mo(median 14.6 mo). CTCs isolated from pre-treatment cryopreserved PBMCs(n = 43) were examined for associations with clinicopathological variables and survival outcomes.RESULTS While there was a significant trend to a decrease in CTC numbers associated with cryopreserved specimens(mean number of CTCs 34.4 vs 51.5, P = 0.04), this was predominately in samples with a total CTC count of > 50, with low CTC count samples less affected(P = 0.06). There was no significant association between the duration of cryopreservation and number of CTCs. In cryopreserved PBMCs from patient samples prior to treatment, a high CTC count(> 17) was associated with poorer overall survival(OS)(n = 43, HR = 4.4, 95%CI: 1.7-11.7, P = 0.0013). In multivariate analysis, after controlling for sex, age, stage, ECOG performance status, and primary tumor location, a high CTC count remained significantly associated with a poorer OS(HR = 3.7, 95%CI: 1.2-12.4, P = 0.03). CONCLUSION PBMC cryopreservation for delayed CTC isolation is a valid strategy to assist with sample collection, transporting and processing. | Daniel Brungs David Lynch Alison WS Luk Elahe Minaei Marie Ranson Morteza Aghmesheh Kara L Vine Martin Carolan Mouhannad Jaber Paul de Souza Therese M Becker | 2018 | World Journal of Gastroenterology2018，24，7： | 5 |
| 2 | Comparison of some French-American hybrid wines with white Riesling using gas chromatography-olfactometry显示文摘 | CHISHOLM MARY G GUIHER L ElSE A VONAH THERESE M | 1994 | The American Journal of Enology and Viticulture1994，45，2： | 1 |
| 3 | Littoral periphyton responses to nitrogen and phosphorus: an experimental study in a subtropical lake显示文摘 | Karl E Therese L E Andrew J R | 1999 | Aquatic Botany1999，，63： | 1 |
| 4 | Tacrolimus for the treatment of fistulas in patients with crohn’s disease: a randomized, placebo-controlled trial显示文摘 | William J Sandborn Daniel H Present Kim L Isaacs Douglas C Wolf Eugene Greenberg Stephen B Hanauer Brian G Feagan Lloyd Mayer Therese Johnson Joseph Galanko Christopher Martin Robert S Sandler | 2003 | Gastroenterology2003，，2： | 1 |
| 5 | Inactivation of Bacteriophage MS2 with potassium ferrate (Ⅵ) 显示文摘 | HU L H MARTIN A P THERESE S | 2012 | Environmental Science & Technology2012，46，12： | 1 |
| 6 | Use of polymerase chain reaction in detection of mycobacterim tuberculosis complex DNA from vitreous sample of Eales disease显示文摘 | Biswas J Therese L Madhavan HN | 1999 | Br J Ophthalmol1999，83，： | 1 |
| 7 | Application of PCR-based restriction fragment length polymorphism (RFLP) for the identification of mycobacterial isolates 显示文摘 | Deepa P Therese K L Madhavan H N | 2005 | Med Res2005，121，： | 1 |
| 8 | Evaluation ofPCR based DNA sequencing targeting mitochondrial larger subunit(mtLSU)region for the rapid detection of Pneumocystis jirovecii insputum from HIV positive patients显示文摘 | Revathy M Therese K L Chandrasekar C | 2012 | BMC Infect Dis2012，12，： | 1 |
| 9 | Application of PCR-based restriction fragment length polymorphism (RFLP) for the identification of mycobacterial isolates 显示文摘 | Deepa P Therese K L Madhavan H N | 2005 | Med Res2005，121，： | 1 |
| 10 | Modulating ccstimulation during antigen presentation to enhance can- cer immunotherapy显示文摘 | Therese L Ines D Alessio L | 2012 | Immu nology2012，12，3： | 1 |
| 11 | The effect of China' s one - child family policy after 25 years 显示文摘 | THERESE H LI L ZHU W X | 2005 | New England Journal of Medi-cine2005，353，11： | 1 |
| 12 | The impact of comorbidity on outcome in 12 623 Danish Head and Neck Cancer Patients: A population based study from the DAHANCA database显示文摘 | Charlotte Rotb? l B? je Susanne O. Dalton Therese K. Gr? nborg Hanne Primdahl Claus A. Kristensen Elo Andersen J? rgen Johansen Lisbeth J. Andersen Jens Overgaard | 2013 | Acta Oncologica2013，，： | 1 |
| 13 | Use of polymerase chain reaction in detection of mycobacterium tuberculosis complex DNA from vitreous sample of Eales' disease显示文摘 | Biswas J Therese L Madhavan HN | 1999 | Br J Ophthalmol1999，83，： | 1 |
| 14 | Carotid stents:Early and intermediate follow-up with Doppler US 显示文摘 | Michelle L Mark E Therese M | 1997 | Radiology1997，205，3： | 1 |
| 15 | The potential role of ultrasound contrast agents I n the liver 显示文摘 | Therese M | 1997 | Applied Radiology1997，26，1： | 1 |
| 16 | New insights towards the function of glutamate dehydrogenase revealed during source-sink transition of tobacco ( Nicotiana tabacum) plants grown under different nitrogen regimes 显示文摘 | Therese T L Gisela M Bertrand H | 2004 | PhysiologiaPlantarum2004，120，： | 1 |
| 17 | Polymerase chain re- action in detection of Mycobacterium tuberculosis complex DNA from vitreous sample of Eales disease 显示文摘 | Madhavan HN Therese L Gurisha P | 2000 | Invest Opthal- mol Vis Sci2000，41，： | 1 |
| 18 | carotid stents Early and in- termediate follow- up with Doppler US显示文摘 | Michelle L Mark E Therese M | 1997 | Radiology1997，205，： | 1 |
| 19 | Use of polymerase chain reaction in detection of Mycobacterium tuberculosis complex DNA from vitreous sample of Eales' disease 显示文摘 | Biswas J Therese L Madhavan HN | 1999 | Br J Ophthalmol1999，83，8： | 1 |
| 20 | Effects of two plant species, L) and tomato(Lycopersicon esculentum Mill), on the diversity of soilborne populations of Fluorescent pseudomonads显示文摘 | Philippe L Therese C Louis G | 1995 | Applied Environmental Microbiology1995，61，3： | 1 |
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