|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 30个重要小麦生产品种抗叶锈性基因分析显示文摘【目的】小麦叶锈病是影响中国小麦产量的重要病害之一,培育持久抗病品种可以经济、有效地控制该病害。论文通过基因推导结合系谱分析、分子标记及成株抗病鉴定对小麦生产品种中抗病基因进行鉴定,从而确定小麦品种中所携带的抗病基因。【方法】选用18个小麦叶锈菌菌系(PHGQ、THJT、PHJT、KHJS、PHJS、THTT?、KHHT、FHRT、FHJQ、PHTT、THTT?、PHTT、FHTR、FHHT?、FHHT?、TGGT、FHTT、FGMT)接种36个已知抗叶锈病基因载体品种和中国的30个小麦生产品种进行苗期抗叶锈病基因推导,进一步利用9个与已知抗病基因紧密连锁的特异性标记进行标记检测,同时系谱分析法确定供试小麦品种中所携带的已知抗叶锈病基因。为了鉴定小麦品种的成株抗性基因,在2014—2015和2015—2016年度将30个小麦品种、慢锈对照品种SAAR和感病对照品种郑州5389种植于河北农业大学小麦试验田和河南周口黄泛区农场试验田,田间用混合生理小种(FHRT、THTT、THJT)接种进行成株抗叶锈性鉴定,进一步运用软件IBM SPSS Statistics 19.0进行方差分析(ANOVA),根据苗期与成株期的侵染型排除具有主效抗性基因的品种,将田间最终严重度(当达到发病高峰时调查的严重度为最终严重度,final disease severity,FDS)明显小于或与慢锈对照SAAR无显著差异的作为慢锈品种,从而筛选出表现慢锈的小麦品种。【结果】基因推导、系谱分析结合标记检测结果表明,30个小麦生产品种中有4个品种(鄂恩5号、鄂麦14、陕229和西农979)含有抗病基因Lr1,10个品种(鄂恩1号、鄂恩5号、鄂恩6号、贵农16、陕225、陕354、陕715、陕合6号、陕麦509和陕农7859)携带有抗病基因Lr26,2个品种(陕225和小偃81)经分子标记检测含有慢锈抗病基因Lr46,另外还有3个品种(西农979、陕229和贵农16)可能含有基因Lr13,所有供试品种均不含Lr9、Lr10、Lr19、Lr20、Lr24和Lr34抗病基因。根据2年2点的田间抗叶锈病鉴定筛选出18个表现慢锈的品种,且方差分析结果表明各品种间和地点间差异均极显著,年份间差异显著,品种与地点间、品种与年份间差异均极显著,而品种与重复间和重复间均不显著,这表明小麦叶锈病抗性的表达受基因型和环境互作共同影响。【结论】30个小麦品种中共检测到Lr1、Lr26、Lr13和Lr46等4个抗叶锈病基因,其中Lr46为成株抗病基因,通过田间抗性鉴定共检测出18个品种可能携带成株慢锈基因,所有慢锈材料中可能含有未知成株抗叶锈病基因,需要进一步进行遗传鉴定。 | 闫晓翠 李在峰 杨华丽 张换换 Gebrewahid Takele Weldu 姚占军 刘大群 周悦 | 2017 | 中国农业科学2017，50，2： | 17 |
| 2 | 70份国外小麦品种(系)的苗期和成株期抗叶锈病鉴定显示文摘小麦叶锈病是小麦生产中的重要病害之一,培育持久抗病品种是最经济、有效和环保的方法。本研究用19个不同毒力的叶锈菌小种苗期接种70份国外引进小麦品种(系)及36个已知抗叶锈病基因的载体品种进行抗性鉴定,同时在2016—2017年度分别于河北保定和河南周口对70份国外引进品种进行田间抗叶锈性鉴定。为进一步检测材料中所携带的苗期和成株抗叶锈病基因,利用12个与已知基因紧密连锁的分子标记进行检测,综合基因推导、系谱分析和分子标记检测的结果,在33份材料中鉴定出15个抗叶锈病基因,包括Lr1、Lr2a、Lr26、Lr3ka、Lr11、Lr17、Lr30、Lr10、Lr14a、Lr2b、Lr13、Lr15、Lr21、Lr44和Lr45,田间鉴定筛选出39份品种表现慢锈性。苗期和田间表现表明,国外品种中含有丰富的对我国叶锈菌小种有效的苗期和成株期抗叶锈病基因,可作为小麦抗叶锈病抗源在抗病育种中加以利用。 | 郑慧敏 温晓蕾 郝晨阳 张培培 GEBREWAHID Takele Weldu 闫晓翠 刘大群 张学勇 李在峰 | 2019 | 作物学报2019，45，10： | 9 |
| 3 | Seedling and adult plant resistance to leaf rust in 46 Chinese bread wheat landraces and 39 wheat lines with known Lr genes显示文摘Wheat leaf rust,caused by Puccinia triticina(Pt),is an important foliar disease that has an important influence on wheat yield.The most economic,safe and effective way to control the disease is growing resistant cultivars.In the present study,a total of 46 wheat landraces and 34 wheat lines with known Lr(leaf rust resistance)genes were inoculated with 16Pt pathotypes for postulating seedling resistance gene(s)in the greenhouse.These cultivars and five wheat differential lines with adult plant resistance(APR)genes(Lr12,Lr22b,Lr34,Lr35 and Lr37)were also evaluated for identification of slow rusting resistance in the field trials in Baoding,Hebei Province of China in the 2014–2015 and 2015–2016 cropping seasons.Furthermore,10 functional molecular markers closely linked to 10 known Lr genes were used to detect all the wheat genotypes.Results showed that most of the landraces were susceptible to most of the Pt pathotypes at seedling stage.Nonetheless,Lr1 was detected only in Hongtangliangmai.The field experimental test of the two environments showed that 38 landraces showed slow rusting resistance.Seven cultivars possessed Lr34 but none of the landraces contained Lr37 and Lr46.Lr genes namely,Lr9,Lr19,Lr24,Lr28,Lr29,Lr47,Lr51 and Lr53 were effective at the whole plant stage.Lr18,Lr36 and Lr45 had lost resistance to part of pathotypes at the seedling stage but showed high resistance at the adult plant stage.Lr34 as a slowing rusting gene showed good resistance in the field.Four race-specific APR genes Lr12,Lr13,Lr35 and Lr37 conferred good resistance in the field experiments.Seven race-specific genes,Lr2b,Lr2c,Lr11,Lr16,Lr26,Lr33 and LrB had lost resistance.The 38 landraces showed slow rusting resistance to wheat leaf rust can be used as resistance resources for wheat resistance breeding in China. | ZHANG Pei-pei Takele Weldu Gebrewahid ZHOU Yue LI Qing-luo LI Zai-feng LIU Da-qun | 2019 | Journal of Integrative Agriculture2019，18，5： | 5 |
| 4 | 小麦品种‘武农148’和‘西农928’的抗叶锈病基因分析显示文摘本研究选用9个小麦叶锈菌菌系接种36个已知基因载体品种(系)、‘武农148’和‘西农928’进行抗叶锈基因苗期推导分析,在2014—2015和2015—2016连续两年两点对其进行成株抗叶锈性鉴定并结合苗期抗叶锈基因推导与系谱分析;利用9个与已知抗病基因紧密连锁的特异性标记进行标记检测,从而得出供试材料的抗性和携带的抗病基因。结果表明,‘西农928’中可能携带抗病基因Lr30、Lr42和携带有未知微效抗病基因,所检测的2个品种均不含抗病基因Lr1、Lr9、Lr10、Lr19、Lr20、Lr24、Lr26、Lr34和Lr46。针对西农928中含有的抗叶锈病基因,可作为小麦抗锈病育种中的抗源材料。 | 闫晓翠 姚世博 Gebrewahid Takele Weldu 姚占军 | 2017 | 河北农业大学学报2017，40，2： | 3 |
| 5 | High-temperature wheat leaf rust resistance gene Lr13 exhibits pleiotropic effects on hybrid necrosis显示文摘Dear Editor,Wheat is one of the most important staple food crops.Leaf rust caused by Puccinia triticina Eriks.(Pt)is a destructive foliar disease that threatens world wheat production.Complementary wheat genes Necrosis 1(Ne1)and Necrosis 2(Ne2)on chromosome arms 5BL and 2BS,respectively,cause hybrid necrosis(Caldwell and Compton,1943;Chu et al.,2006).Ne2 shows extremely tight genetic linkage with leaf rust resistance gene Lr13(Zhang et al.,2016).We previously identified a temperature-sensitive leaf rust resistance gene LrZH22 in Chinese wheat cultivar Zhoumai 22(ZM,Figure 1A)that was effective against most Chinese Pt pathotypes(Wang et al.,2016)and shared an overlapping genetic interval with Lr13.In a separate study,a recessive early leaf senescence 1(els1)gene(Figure 1B)shared an overlapping interval with LrZH22/Lr13/Ne2(Chu et al.,2006;Wang et al.,2016;Zhang et al.,2016;Li et al.,2018).However,the function and relationship of LrZH22/Lr13/Ne2/els1 remained unknown for decades.Here,we report the map-based cloning of LrZH22 and els1 and characterize their relationship with Lr13 and Ne2 in conjunction with a companion paper(Hewitt et al.t 2021)studying the same gene. | Xiaocui Yan Miaomiao Li Peipei Zhang Guihong Yin Huaizhi Zhang Takele Weldu Gebrewahid Jianping Zhang Lingli Dong Daqun Liu Zhiyong Liu Zaifeng Li | 2021 | Molecular Plant2021，14，7： | 2 |
| 6 | QTL mapping of adult plant resistance to stripe rust and leaf rust in a Fuyu 3/Zhengzhou 5389 wheat population显示文摘Stripe or yellow rust(YR)and leaf rust(LR)cause large losses in wheat production worldwide.Resistant cultivars curtail the levels of losses.The present study aimed to identify quantitative trait loci(QTL)for YR and LR resistance in 147 F2:6 recombinant inbred lines(RIL)derived from the cross Fuyu 3/Zhengzhou 5389.The RIL population and parents were genotyped with the Wheat55 K single nucleotide polymorphism(SNP)array and simple sequence repeat(SSR)markers.All materials were also phenotyped for YR severity at Mianyang in Sichuan province and Baoding in Hebei province in the 2015/2016,2016/2017,and 2017/2018 cropping seasons,and LR severity at Zhoukou in Henan province and at Baoding in 2017/2018.Eleven QTL for YR resistance and five for LR resistance were detected using inclusive composite interval mapping(Ici Mapping).Four of these QTL on chromosomes 1 BL,2 BS,3 AL,and 5 AL conferred resistance to both YR and LR.The QTL on 1 BL was Lr46/Yr29,and that on 7 BL might be Lr68.The QTL on chromosome 2 BS was detected at a similar position to previously detected loci.QYr.hebau-3 AL/QLr.hebau-3 AL,QYr.hebau-5 AL/QLr.hebau-5 AL,QYr.hebau-7 DL,QYr.hebau-4 BS,QYr.hebau-6 DL,and QYr.hebau-2 AS are likely to be new.An SSR marker for QYr.hebau-7 DL was developed and validated in a diverse wheat panel from China,suggesting effectiveness in different genetic backgrounds.These QTL with closely linked SNP and SSR markers could be useful for marker-assisted selection in wheat breeding programs targeting durable resistance to both diseases. | Takele Weldu Gebrewahid Peipei Zhang Yue Zhou Xiaocui Yan Xianchun Xia Zhonghu He Daqun Liu Zaifeng Li | 2020 | The Crop Journal2020，8，4： | 2 |
| 7 | 69份国外小麦品种(系)抗叶锈性鉴定及基因分析显示文摘为给抗叶锈病育种提供优异抗源,选用17个不同毒力的叶锈菌生理小种,对69份国外小麦品种(系)及36份已知抗病基因的近等基因系进行苗期抗叶锈病基因推导,并结合11个已知基因的分子标记进行标记检测;于2017-2018年度分别在河北保定试验田和河南周口黄泛区农场对69份国外小麦材料进行田间成株期抗叶锈性鉴定。结果发现,在69份国外小麦品种(系)中共检测到6个抗小麦叶锈病基因(Lr1、Lr10、Lr19、Lr26、Lr34和Lr37),携带苗期抗病基因Lr1、Lr10、Lr19和Lr26的分别有6、19、5和10份品种(系),携带成株抗病基因Lr34和Lr37的分别有10和26份品种(系),田间鉴定具有成株慢锈性的品种(系)共有46份。 | 王思曼 张静 张培培 Gebrewahid Takele Weldu 闫晓翠 刘大群 李在峰 | 2020 | 麦类作物学报2020，40，10： | 2 |
| 8 | Identification of leaf rust resistance genes in common wheat varieties from China and foreign countries显示文摘Wheat leaf rust,triggered by Puccinia triticina Eriks(Pt),is among the most important diseases of wheat worldwide.Deploying resistant varieties against leaf rust is the most effective,environmentally-friendly and economic way to control the disease.In the present study,66 wheat varieties form China and foreign countries were tested with 17 Pt races for gene postulation during the seedling stage in the greenhouse.All the varieties were also planted to identify slow rusting responses to leaf rust at the adult plant stage in Baoding and Zhoukou field trials during the 2016/2017 to 2017/2018 cropping seasons.Moreover,12 closely linked molecular markers to known leaf rust resistance(Lr)genes were used for assessing all the varieties.The results of both gene postulation and molecular marker identification showed that a total of eight Lr genes,Lr1,Lr10,Lr17,Lr20,Lr26,Lr34,Lr37 and Lr46,either singly or in combination were detected in 32 varieties.Known Lr genes were not identified in the remaining 34 varieties.Seventeen varieties were found to have slow rusting resistance.The resistance sources identified in this study can be used as resources for resistance against leaf rust in wheat breeding programs in China and the respective foreign countries. | LIU Yuan Takele Weldu GEBREWAHID ZHANG Pei-pei LI Zai-feng LIU Da-qun | 2021 | Journal of Integrative Agriculture2021，20，5： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员