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| 1 | MiR-122-5p suppresses the proliferation, migration, and invasion of gastric cancer cells by targeting LYN显示文摘Gastric cancer(GC)is one of malignant tumors with high mortality and morbidity in the world.MicroRNA-122(miR-122)acts as a tumor suppressor in a variety of cancers and has been found to be dominant in gastric adenocarcinoma.However,the specific biological function of miR-122-5p in GC is not completely clear.In this study,we found that miR-122-5p was low-expressed in GC tissues and cell lines by using qRT-PCR.Overexpression of miR-122-5p inhibited the proliferation,migration,and invasion of GC cells by using CCK-8 and transwell assays.On the contrary,downregulation of miR-122-5p promoted the proliferation,migration,and invasion of GC cells.In addition,we found that the expression of LYN,an Src family tyrosine kinase,was inversely correlated with miR-122-5p expression in GC tissues by using western blot analysis,immunohistochemistry,and qRT-PCR assays.Meanwhile,luciferase assay results indicated that LYN is a direct target of miR-122-5p in GC cells.Moreover,silencing LYN expression by its siRNA inhibited the proliferation,migration,and invasion of GC cells.Importantly,overexpression of LYN restored miR-122-5p-mediated inhibition of the proliferation,migration,and invasion of GC cells.Taken together,our results indicated miR-122-5p inhibited the proliferation,migration,and invasion by targeting LYN in GC. | Lei Meng Zhangming Chen Zhe Jiang Ting Huang Jie Hu Panquan Luo Hongli Zhang Mengqi Huang Lei Huang Yu Chen Ming Lu A-man Xu Songcheng Ying | 2020 | Acta Biochimica et Biophysica Sinica2020，52，1： | 12 |
| 2 | Development of a rapid and sensitivity magnetic chemiluminescence immunoassay for DNA methyltransferase 1 in human serum显示文摘DNA methyltransferase 1(DNMT1)is a useful biomarker for lung cancer in early clinical diagnosis.A rapid magnetic chemiluminescence immunoassay(MCLIA)for DNMT1 in human serum has been developed.Horseradish peroxidase(HRP)-second-Ab was used to labeled polyclonal antibodies of anti-DNMT1.DNMT1 in sample integrates with specific immunomagnetic beads and can constitute a supersandwiched immunoreaction.In magnetic field,nonspecific materials can be separated.After luminescent substrate luminol-H2O2-BIP was added,the relative light unit(RLU)of HRP was detected and was discovered to be directly proportional to the content of DNMT1 in sample.The correlative variables involved in the MCLIA value were optimized and the methodological evaluation was carried out.After optimization,in the range of0.5–128 ng/mL,the linear regression equation was y=0.5014 x+1.769(x was logCDNMT1,y was relative luminescence units(RLU)/RLU0),and the limit of detection was 0.01 ng/mL.The RSD of intra-and interassays were 15.8%–16.9%and 14.3%–18.1%,respectively.The recovery was from 70.0%to 106.2%.Furthermore,paralleled with purchasable enzyme-linked immunosorbent assay(ELISA)kits,MCLEIA had lower detection limit,wider linear range and shorter detection time.Therefore,the MCLEIA established in this study could be used for the sensitive detection of DNMT1 in serum sample. | Sitian He Leiliang He Beibei Liu Songcheng Yu Li'e Liu Yongmei Tian Jia Wang Lihua Ding Yilin Wang Lingbo Qu Fei Yu Yongjun Wu | 2019 | Chinese Chemical Letters2019，30，5： | 4 |
| 3 | A competitive chemiluminescence enzyme immunoassay for rapid and sensitive determination of enrofloxacin显示文摘 | Fei Yu Yongjun Wu Songcheng Yu Huili Zhang Hongquan Zhang Lingbo Qu Peter de B. Harrington | 2012 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy2012，，： | 1 |
| 4 | The pretreatment effects on the antioxidant activity of jujube polysaccharides显示文摘 | QU Cbenling YU Songcheng JIN Huali | 2013 | Spectrochim Acta A2013，114，： | 1 |
| 5 | Cell-specific Regulation of APOBEC3F by Interferons显示文摘人的 cytidine 脱氨基酶 APOBEC3F (A3F ) 包括人的免疫不全病毒类型 1 对肝炎 B 病毒和制动火箭病毒举办宽广抗病毒的活动。然而,它在病毒的自然目标房间的规定如此的 CD4+T 淋巴细胞,巨噬细胞,和主要的肝房间一直不好学习。这里,我们证明 A3F 由在主要 hepatocytes 和多重肝房间的 -alpha 象巨噬细胞一样衬里的干扰素(IFN ) 是起来调整的。尽管 IFN-alphasignaling 小径在 T 淋巴的房间是活跃的,另外的 IFN 的正式就职刺激了基因,如此的一个说话者被检测, A3F 和 APOBEC3G (A3G ) 没被 IFN-oc 在这些房间导致。因此,除已知的刺激 IFN 的基因以外的另外的因素也调整了 IFN-alpha-induced A3F 特别在 IFN-alphastimulation 以后,在主要 hepatocytes 的 expressiondistinctly.A3F 和 A3G 表示层次比得上在在一些个人的 CD4+T 淋巴细胞的那些。在从各种各样的题目的主要 hepatocytes 的 A3F 和 A3G 表示的重要变化是在 A3F 或 A3G 规定的 observed.Individualvariations,表示可能影响肝炎 B 感染的临床的结果。 | Songcheng YING Xuzhao ZHANG Phuong Thi Nguyen SARKIS Rongzhen XU Xiaofang YU | 2007 | Acta Biochimica et Biophysica Sinica2007，39，4： | 0 |
| 6 | Pd-catalyzed cascade cyclization of allenylethylene carbonates and indandiones: Synthesis of tetracyclic dihydrocyclopentaindenofuranone derivatives显示文摘A palladium-catalyzed cascade cyclization of allenylethylene carbonates with 1,3-indandiones was developed, providing biologically interesting tetracyclic dihydrocyclopentaindenofuranone derivatives having three contiguous quaternary carbon centers in moderate to high yields with excellent diastereoselectivities. In this reaction, the allene moiety was fully fused into the cyclopentene ring. | Yujie Dong Jun Liu Xing Gao Ting Pan Biming Mao Songcheng Yu Yongjun Wu Cheng Zhang Hongchao Guo | 2023 | Chinese Chemical Letters2023，34，10： | 0 |
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