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| 1 | ZmMs30 Encoding a Novel GDSL Lipase Is Essential for Male Fertility and Valuable for Hybrid Breeding in Maize显示文摘Genic male sterility (GMS) is very useful for hybrid vigor utilization and hybrid seed production. Although a large number of GMS genes have been identified in plants, little is known about the roles of GDSL lipase members in anther and pollen development. Here, we report a maize GMS gene, ZmMs30, which encodes a novel type of GDSL lipase with diverged catalytic residues. Enzyme kinetics and activity assays show that ZmMs30 has lipase activity and prefers to substrates with a short carbon chain. ZmMs30 is specifically expressed in maize anthers during stages 7-9. Loss of ZmMs30 function resulted in defective anther cuticle, irregular foot layer of pollen exine, and complete male sterility. Cytological and lipidomics analyses demonstrate that ZmMs30 is crucial for the aliphatic metabolic pathway required for pollen exine formation and anther cuticle development. Furthermore, we found that male sterility caused by loss of ZmMs30 function was stable in various inbred lines with different genetic background, and that it didn't show any negative effect on maize heterosis and production, suggesting that ZmMs30 is valuable for crossbreeding and hybrid seed production. We then developed a new multi-control sterility system using ZmMs30 and its mutant line, and demonstrated it is feasible for generating desirable GMS lines and valu. able for hybrid maize seed production. Taken together, our study sheds new light on the mechanisms of anther and pollen development, and provides a valuable male-sterility system for hybrid breeding maize. | Xueli An Zhenying Dong Youhui Tian Ke Xie Suowei Wu Taotao Zhu Danfeng Zhang Van Zhou Canfang Niu Biao Ma Quancan Hou Jianxi Bao Simiao Zhang Ziwen Li Yanbo Wang Tingwei Yan Xiaojing Sun Yuwen Zhang Jinping Li Xiangyuan Wan | 2019 | Molecular Plant2019，12，3： | 15 |
| 2 | Effect of contact- and sliding-mode electrification on nanoscale charge transfer for energy harvesting显示文摘费用转移的过程最近作为收获技术的一个新、有希望的精力的基础基于 triboelectrification (TE ) 和接触充电(CE ) 被利用了,即,摩擦电 nanogenerators,以及 selfpowered 传感器和系统。在二表面之间的静电的费用转移能发生在 TE 和取决于相对滑动磨擦的参与的 CE 模式。在 TE 的滑动行为从 CE 在费用转移导致任何基本差别吗?因为挑战,很少研究在这比较上是可得到的。这份报纸基于扫描探查在 nanoscale 在 CE 和 TE 在基本差别上提供第一研究显微镜的方法。在相等的接触时间和力量的二进程的量的比较被提供,并且结果建议从 TE 的费用转移从 CE,而是转移费用密度的浸透值是比那快得多的是一样。11 eV 的测量摩擦精力驱散尖端什么时候在 1 的距离上扫描?使潜在的机制清楚些:磨擦可以经由电子刺激便于费用转移过程。这些结果提供基本指导让材料和设备结构的选择在不同应用启用 TE 或 CE;CE 模式为象收获的颤动精力那样的经常的中等接触是有利的, TE 模式为象从人的走精力收获那样的立刻的运动是有利的。 | Yu Sheng Zhou Shengming Li Simiao Niu Zhong Lin Wang | 2016 | Nano Research2016，9，12： | 5 |
| 3 | Strengthening population medicine to promote public health显示文摘As we enter.2022,we are facedwith the fact that coronavirus disease2019(CovID-19)has been devastating the world formore than 2full years.Since the original outbreak of COVID-19in December2019,the disease has proved to be a major threat to global health and,perhaps more importantly,one that is not likely to be resolved soon.Severe_acute respiratory syndrome coronavirus. | Chen Wang Simiao Chen Guangliang Shan Zhiwei Leng Till Bärnighausen Weizhong Yang | 2022 | Chinese Medical Journal2022，，10： | 3 |
| 4 | The impact of UV treatment on microbial control and DBPs formation in full-scale drinking water systems in northern China显示文摘To manage potential microbial risks and meet increasingly strict drinking water health standards,UV treatment has attracted increasing attention for use in drinking water systems in China.However,the effects of UV treatment on microbial control and disinfection byproducts(DBPs)formation in real municipal drinking water systems are poorly understood.Here,we collected water samples from three real drinking water systems in Beijing and Tianjin to investigate the impacts of UV treatment on microbial control and DBP formation.We employed heterotrophic plate count(HPC),flow cytometry(FCM),quantitative PCR analysis,and high-throughput sequencing to measure microorganisms in the samples.Different trends were observed between HPC and total cell count(measured by FCM),indicating that a single indicator could not reflect the real degree of biological re-growth in drinking water distribution systems(DWDSs).A significant increase in the 16S rRNA gene concentration was observed when the UV system was stopped.Besides,the bacterial community composition was similar at the phylum level but differed markedly at the genera level among the three DWDSs.Some chlorine-resistant bacteria,including potential pathogens(e.g.,Acinetobacter)showed a high relative abundance when the UV system was turned off.It can be concluded that UV treatment can mitigate microbial re-growth to some extent.Finally,UV treatment had a limited influence on the formation of DBPs,including trihalomethanes,haloacetic acids,and nitrogenated DBPs.The findings of this study may help to understand the performance of UV treatment in real drinking water systems. | Xiuwei Ao Zhongyun Chen Simiao Li Chen Li Zedong Lu Wenjun Sun | 2020 | Journal of Environmental Sciences2020，32，1： | 2 |
| 5 | Crystal structure of an LRR protein with two solenoids显示文摘 | Peiyuan Liu Zehan Hu Bin Zhou Simiao Liu Jijie Chai | 2013 | Cell Research2013，23，2： | 2 |
| 6 | Factors Predicting Progression to Severe COVID-19: A Competing Risk Survival Analysis of 1753 Patients in Community Isolation in Wuhan, China显示文摘Most studies of coronavirus disease 2019(COVID-19)progression have focused on the transfer of patients within secondary or tertiary care hospitals from regular wards to intensive care units.Little is known about the risk factors predicting the progression to severe COVID-19 among patients in community iso-lation,who are either asymptomatic or suffer from only mild to moderate symptoms.Using a multivari-able competing risk survival analysis,we identify several important predictors of progression to severe COVID-19—rather than to recovery—among patients in the largest community isolation center in Wuhan,China from 6 February 2020(when the center opened)to 9 March 2020(when it closed).All patients in community isolation in Wuhan were either asymptomatic or suffered from mild to moderate COVID-19 symptoms.We performed competing risk survival analysis on time-to-event data from a cohort study of all COVID-19 patients(n=1753)in the isolation center.The potential predictors we inves-tigated were the routine patient data collected upon admission to the isolation center:age,sex,respira-tory symptoms,gastrointestinal symptoms,general symptoms,and computed tomography(CT)scan signs.The main outcomes were time to severe COVID-19 or recovery.The factors predicting progression to severe COVID-19 were:male sex(hazard ratio(HR)=1.29,95%confidence interval(CI)1.04–1.58,p=0.018),young and old age,dyspnea(HR=1.58,95%CI 1.24–2.01,p<0.001),and CT signs of ground-glass opacity(HR=1.39,95%CI 1.04–1.86,p=0.024)and infiltrating shadows(HR=1.84,95%CI 1.22–2.78,p=0.004).The risk of progression was found to be lower among patients with nausea or vomiting(HR=0.53,95%CI 0.30–0.96,p=0.036)and headaches(HR=0.54,95%CI 0.29–0.99,p=0.046).Our results suggest that several factors that can be easily measured even in resource-poor set-tings(dyspnea,sex,and age)can be used to identify mild COVID-19 patients who are at increased risk of disease progression.Looking for CT signs of ground-glass opacity and infiltrating shadows may be an affordable option to support triage decisions in resource-rich settings.Common and unspecific symptoms(headaches,nausea,and vomiting)are likely to have led to the identification and subsequent community isolation of COVID-19 patients who were relatively unlikely to deteriorate.Future public health and clinical guidelines should build on this evidence to improve the screening,triage,and monitoring of COVID-19 patients who are asymtomatic or suffer from mild to moderate symptoms. | Simiao Chen Hui Sun Mei Heng Xunliang Tong Pascal Geldsetzer Zhuoran Wang Peixin Wu Juntao Yang Yu Hu Chen Wang Till Bärnighausen | 2022 | Engineering2022，8，6： | 1 |
| 7 | On Technology and Ritual of Chuandou House Construction in Southwest China:The Case of Dong Minority Area显示文摘This study is based on field work conducted in the mountainous countryside of Dong minority area,southwest China,where the custom of chuandou house construction has been preserved relatively completely.In this paper,we try to depict the whole construction process of traditional chuandou dwellings,from the very beginning of material preparation to the completion of one house,by explaining some details of the various steps of the construction process,including design principles,rituals,characteristic details on engineering,etc.During our research,we followed the work of a group of chief carpenters residing in the Dong minorities area.Ritual and technology are two arms helping them to maintain their professional authority towards other people involved in the construction process.Furthermore,we examine the relationship between ritual and technology,how the two can be combined,how they influence the appearance of houses and how one can master them and pass down their knowledge and know-how to future generations of carpenters. | Congcong Ren Ruchen Bian Simiao Li | 2018 | Built Heritage2018，2，1： | 1 |
| 8 | Athermal silicon arrayed waveguide grating with polymer-filled slot structure显示文摘 | Wang Xiang Xiao Simiao Zheng Weiwei | 2009 | Optics Communications2009，282，： | 1 |
| 9 | Cryogenic sample grinding for copper,lead and manganesedetermination in human teeth by slurry sampling GFAAS显示文摘 | Santos Junior D Barbosa Junior F Simiao de Souza S | 2003 | J Anal Atom Spectrom2003，18，8： | 1 |
| 10 | Piezoresistive effect in MoO3 nanobelts and its application in strain-enhanced oxygen sensors显示文摘有不同性质的 MoO3 nanobelts (NB ) 经由物理蒸汽免职(PVD ) 被综合了方法。NB 的结晶的结构和形态学被 X 光检查衍射,传播电子显微镜学和扫描电子显微镜学描绘。电的大小被执行,在 MoO3 的深刻 piezoresistive 效果试验性地学习了并且验证。影响计量器因素的因素例如 NB 尺寸,做的集中和空气作文,被讨论并且分析。煤气的察觉到性能也在设备被测试,由把紧张用于煤气的传感器,它的察觉到的表演能有效地被调节并且提高,这被表明。这研究在 MoO3 NB 和另外的电子调整测量负担得起更好的设备性能和更宽广的材料功能的这效果能借助于被结合的通用机制的第一幅插图提供重要 piezoresistivity 的第一示范。 | Xiaonan Wen Weiqing Yang Yong Ding Simiao Niu Zhong Lin wang | 2014 | Nano Research2014，7，2： | 1 |
| 11 | The mutual information based correlation analysis between fault types and monitor data 显示文摘 | LIN Huo CHUAN Lv DONG Zhou SIMIAO F | 2011 | Procedia Engineering2011，，： | 1 |
| 12 | Dimension-Enhanced Ultra-High Performance Liquid Chromatography/Ion Mobility-Quadrupole Time-of-Flight Mass Spectrometry Combined with Intelligent Peak Annotation for the Rapid Characterization of the Multiple Components from Seeds of Descurainia sophia显示文摘The complex composition of herbal metabolites necessitates the development of powerful analytical techniques aimed to identify the bioactive components.The seeds of Descurainia sophia(SDS)are utilized in China as a cough and asthma relieving agent.Herein,a dimension-enhanced integral approach,by combining ultra-high performance liquid chromatography/ion mobility-quadrupole time-of-flight mass spectrometry(UHPLC/IMQTOF-MS)and intelligent peak annotation,was developed to rapidly characterize the multicomponents from SDS.Good chromatographic separation was achieved within 38 min on a UPLC CSH C18(2.1×100 mm,1.7μm)column which was eluted by 0.1%formic acid in water(water phase)and acetonitrile(organic phase).Collision-induced dissociation-MS^(2)data were acquired by the data-independent high-definition MS^(E)(HDMS^(E))in both the negative and positive electrospray ionization modes.A major components knockout strategy was applied to improve the characterization of those minor ingredients by enhancing the injection volume.Moreover,a self-built chemistry library was established,which could be matched by the UNIFI software enabling automatic peak annotation of the obtained HDMS^(E)data.As a result of applying the intelligent peak annotation workflows and further confirmation process,a total of 53 compounds were identified or tentatively characterized from the SDS,including 29 flavonoids,one uridine derivative,four glucosides,one lignin,one phenolic compound,and 17 others.Notably,four-dimensional information related to the structure(e.g.,retention time,collision cross section,MS^(1)and MS^(2)data)was obtained for each component by the developed integral approach,and the results would greatly benefit the quality control of SDS. | Simiao Wang Xue Li Boxue Chen Shitong Li Jiali Wang Jing Wang Mingshuo Yang Xiaoyan Xu Hongda Wang Wenzhi Yang | 2022 | Phyton-International Journal of Experimental Botany2022，91，3： | 1 |
| 13 | Headspace Solid-Phase Micro-Extraction Gas Chromatography/Mass Spectrometry(HS-SPME-GC/MS)-Based Untargeted Metabolomics Analysis for Comparing the Volatile Components from 12 Panax Herbal Medicines显示文摘Quality control of ginseng currently is mainly based on ginsenoside analysis,but rarely focuses on the volatile organic components.In the current work,an untargeted metabolomics approach,by headspace solid-phase micro-extraction gas chromatography/mass spectrometry(HS-SPME-GC/MS),was elaborated and further employed to holistically compare the compositional difference of the volatile components simultaneously from 12 Panax herbal medicines,which included P.ginseng(PG),P.quinquefolius(PQ),P.notoginseng(PN),red ginseng(PGR),P.ginseng leaf(PGL),P.quinquefolius leaf(PQL),P.notoginseng leaf(PNL),P.ginseng flower(PGF),P.quinquefolius flower(PQF),P.notoginseng flower(PNF),P.japonicus(PJ),and P.japonicus var.major(PJvm).Chromatographic separation was performed on an HP-5MS elastic quartz capillary column using helium as the carrier gas,enabling good resolution within 1 h.We were able to characterize totally 259 volatile compounds,including 82 terpenes(T),46 alcohols(Alc),29 ketones(K),25 aldehydes(Ald),21 esters(E),and the others.By analyzing 90 batches of ginseng samples based on the untargeted metabolomics workflows,236 differential ions were unveiled,and accordingly 36 differential volatile components were discovered.It is the first report that simultaneously compares the compositional difference of volatile components among 12 Panax herbal medicines,and useful information is provided for the quality control of ginseng aside from the well-known ginsenosides. | Simiao Wang Xiaohang Li Meiting Jiang Xinlong Wu Yuying Zhao Meiyu Liu Xiaoyan Xu Huimin Wang Hongda Wang Heshui Yu Wenzhi Yang | 2022 | Phyton-International Journal of Experimental Botany2022，91，7： | 1 |
| 14 | Hardware-in-the-Loop Simulation System for Space Manipulator Docking: Model,Stability and Experimental Evaluation显示文摘A manipulator-type docking hardware-in-the-loop(HIL)simulation system is proposed in this paper,with further development of the space docking technology and corresponding requirements of the engineering project.First,the structure of the manipulator-type HIL simulation system is explained.The mass and the flexibility of the manipulator has an important influence on the stability of the HIL system,which is the premise of accurately simulating actual space docking.Thus,the docking HIL simulation models of rigid,flexible and flexible-light space manipulators are established.The characteristics of the three HIL systems are studied from three important aspects:the system parameter configuration relation,the system stability condition and the dynamics frequency simulation ability.The key conclusions obtained were that the system satisfies stability or reproduction accuracy.Meanwhile,the influence of different manipulators on the system stability is further analyzed.The accuracy of the calculated results is verified experimentally. | Simiao Yu Shutao Zheng Yu Yang Zhiyong Qu Junwei Han | 2020 | Journal of Beijing Institute of Technology2020，29，1： | 1 |
| 15 | Hyperdense artery sign, symptomatic infarct swelling and effect of alteplase in acute ischaemic stroke显示文摘Background Alteplase improves functional outcomes of patients with acute ischaemic stroke,but its effects on symptomatic infarct swelling,an adverse complication of stroke and the influence of CT hyperdense artery sign(HAS)are unclear.This substudy of the Third International Stroke Trial aimed to investigate the association between HAS and symptomatic infarct swelling and effect of intravenous alteplase on this association.Methods We included stroke patients whose prerandomisation scan was non-contrast CT.Raters,masked to clinical information,assessed baseline(prerandomisation)and follow-up(24-48 hours postrandomisation)CT scans for HAS,defined as an intracranial artery appearing denser than contralateral arteries.Symptomatic infarct swelling was defined as clinically significant neurological deterioration≤7 days after stroke with radiological evidence of midline shift,effacement of basal cisterns or uncal herniation.Results Among 2961 patients,HAS presence at baseline was associated with higher risk of symptomatic infarct swelling(OR 2.21;95% CI 1.42 to 3.44).Alteplase increased the risk of swelling(OR 1.69;95% CI 1.11 to 2.57),with no difference between patients with and those without baseline HAS(p=0.49).In patients with baseline HAS,alteplase reduced the proportion with HAS at follow-up(OR 0.67;95% CI 0.50 to 0.91),where HAS disappearance was associated with reduced risk of swelling(OR 0.25,95% CI 0.14 to 0.47).Conclusion Although alteplase was associated with increased risk of symptomatic infarct swelling in patients with or without baseline HAS,it was also associated with accelerated clearance of HAS,which in return reduced swelling,providing further mechanistic insights to underpin the benefits of alteplase. | Simiao Wu Grant Mair Geoff Cohen Zoe Morris Anders von Heijne Nick Bradey Lesley Cala Andre Peeters Andrew J Farrall Alessandro Adami Gillian Potter Ming Liu Richard I Lindley Peter A G Sandercock Joanna M Wardlaw the IST-3 Collaborative Group | 2021 | Stroke & Vascular Neurology2021，6，2： | 1 |
| 16 | An engineered platform for reconstituting functional multisubunit SCF E3 ligase in vitro显示文摘Multisubunit SKP1/Cullin1/F-box(SCF)E3 ligases play essential roles in regulating the stability of crucial regulatory factors and controlling growth and development in eukaryotes.Detecting E3 ligase activity in vitro is important forexploring the molecular mechanism of protein ubiquitination.However,in vitro ubiquitination assay systems for multisubunit E3 ligases remain difficult to achieve,especially in plants,mainly owing to difficulties in achieving active components of multisubunit E3 ligases with high purity and characterizing specific E2 and E3 pairs.In this study,we characterized components of the rice ScFDiwARF3(SCFDs)E3 ligase,screened the coordinated E2,and reconstituted active ScFD3 E3 ligase in vitro.We further engineered SCFD3 E3 ligase using a fused SKP1-Cullin1-RBX1(eSCR)protein and found that both the wild-type SCFD3 E3 ligase and the engineered SCFD3 E3 ligase catalyzed ubiquitination of the substrate D53,which is the key transcriptional repressor in strigolactone signaling.Finally,we replaced D3 with other F-box proteins from rice and humans and reconstituted active escF E3 ligases,including escFaID2,escFBxL1s,and escFcDC4 E3 ligases.Our work reconstitutes functional SCF E3 ligases in vitro and generates an engineered system with interchangeable F-box proteins,providing a powerful platform for studying the mechanisms of multisubunit SCF E3 ligases in eukaryotes. | Huihui Liu Simiao Liu Hong Yu Xiahe Huang Yingchun Wang Liang Jiang Xiangbing Meng Guifu Liu Mingjiang Chen Yanhui Jing Feifei Yui Bing Wang Jiayang Li | 2022 | Molecular Plant2022，15，8： | 1 |
| 17 | Edaravone improves functional and structural outcomes in animal models of focal cerebral ischemia:A systematic review显示文摘 | Simiao Wu Emily Sena Kieren Egan | 2014 | International Journal of Stroke2014，9，： | 1 |
| 18 | Simultaneous determination of nineteen major components in Qi She Pill by ultra-high-performance liquid chromatography–tandem mass spectrometry显示文摘Qi She Pill(QSP)is a traditional Chinese medicine(TCM)prescription that has been used in treating cervical spondylosis radiculopathy for many years.In this study,a simple and sensitive method using ultra-high-performance liquid chromatography–tandem mass spectrometry(UHPLC–MS/MS)on a reverse-phase C18 column was developed for the simultaneous determination of the 19 major components in QSP.We found that the optimum mobile phase for gradient elution was 0.1% formic acid and methanol.The correlation coefficients of all calibration curves were greater than 0.99.Recoveries measured at three concentration levels varied from 95.43%to 102.35%.Relative standard deviations of intra-and inter-day precisions were less than 4.45%.After successfully validating our method,we then applied it to the quantification of 19 components in QSP products to show that this method provides a new standard in quality assessment of TCM prescriptions containing multiple bioactive components. | Zhongliang Zhang Qiang Li Qiufen Li Simiao Du Yongquan Zhou Chunming Lv Yan Zhao Yongjun Wang Ning Zhang | 2014 | Acta Pharmaceutica Sinica B2014，4，5： | 1 |
| 19 | Edaravone improves functional and structural outcomes in animal models of focal cerebral ischemia: A systematic review显示文摘 | Simiao Wu Emily Sena Kieren Egan Malcolm Macleod Gillian Mead | 2014 | Int J Stroke2014，，1： | 1 |
| 20 | Discharge voltage behavior of electric double-layer capacitors during high-g impact and their application to autonomously sensing high-g accelerometers显示文摘在这研究,电的双层电容器(EDLC ) 的分泌物电压行为理论上并且试验性地在 high-g 影响期间被学习。微规模的动态机制被建议在 high-g 影响期间描述分泌物电压的增加的物理基础。基于这动态机制,一个多地模型被建立,并且模拟和分泌物电压增加现象的试验性的研究被进行。从模拟和试验性的数据,在增加的电压和 high-g 加速之间的关系被揭示。一个加速察觉范围多达, 10,000g 被验证。设备的设计被学习参数的影响优化,例如电极厚度和分泌物电流,在产量上。这个工作基于精力存储设备为自治传感器系统的开发开创新大街并且为许多实际应用程序例如是重要的在测试的碰撞和汽车安全。 | Keren Dai Xiaofeng Wang Fang Yi Yajiang Yin Cheng Jiang Simiao Niu Qingyu Li Zheng You | 2018 | Nano Research2018，11，2： | 1 |
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