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| 1 | Diverse modes of clonal evolution in HBV-related hepatocellular carcinoma revealed by single-cell genome sequencing显示文摘Hepatocellular 癌(HCC ) 是癌症实质词法,基因并且 phenotypic 差异。然而,我们不理解在 intratumor 异质和肿瘤的联系词法 / 组织学的特征之间的关系。用介绍 96 个肿瘤房间(30-36 各个) 和 15 个正常的肝房间(5 各个) 的单个房间的整个染色体的定序,与联系 HBV 的 HCC 从三个男病人收集了,我们证实拷贝数字变化在 hepatocarcinogenesis 早发生,但是此后在整个肿瘤仍然保持相对稳定前进。重要地,我们证明特定的 HCC 能具有 monoclonal 或 polyclonal 起源。有汇合的 multinodular 形态学的肿瘤是典型 polyclonal 肿瘤并且显示最高的 intratumor 异质。除了 mutational 和拷贝数字侧面,我们把了用导出 HBV 的外国 genomic 标记的 HCC 的同种细胞的起源。在 monoclonal HCC,所有肿瘤单身者房间展出一样的 HBV 集成,显示 HBV 集成是一个早司机事件并且在肿瘤前进期间仍然保持极其稳定。另外,我们的结果显示那两个都转移,迟了的传播并且早播种当模特儿,在 HCC 有一个角色前进。尤其是,开始的克隆传播的早 intrahepatic 导致同步 multifocal 肿瘤的形成。同时,我们在 HCC 识别了潜在的司机基因 ZNF717,它展出变化的高频率在单个房间并且人口铺平,,通过调整 IL-6/STAT3 小径行动的肿瘤 suppressor。这些调查结果热点多重不同肿瘤在 HCC 的进化机制,它为特定的处理策略建议需要。 | Meng Duan, Shu Zhang, Zhichao Wang, Jieyi Shi, Longzi Liu, Xiaoying Wang, Aiwu Ke, Jian Zhou, Jia Fan, Qiang Gao Junfeng Hao, Chong Li Sijia Cui Daniel L Worthley Ya Cao Ruibin Xi Xiaoming Zhang Jian Zhou, Jia Fan Qiang Gao | 2018 | Cell Research2018，28，3： | 21 |
| 2 | Effect of Bufei granule on stable chronic obstructive pulmonary disease: a randomized, double blinded, placebo-controlled, and multicenter clinical study显示文摘OBJECTIVE: To study the therapeutic effect Bufei granule, which is a traditional Chinese drug that can enhance the immune function of the lung, on patients with stable chronic obstructive pulmonary disease(COPD).METHODS: This is a randomized, double blinded,placebo-controlled, and multicenter clinical study.Three medical centers in Tianjin, China, participated in the trial. A total of 140 patients with stable COPD were enrolled and randomized into two groups, with 70 patients in each. The treatment group was treated with Bufei granule, while the control group received Bufei placebo. The pharmacological treatment lasted for 12 weeks from the date of enrollment. Then, the indexes of patients were observed. Data were analyzed to study the effect of Bufei granule, with the frequency of acute exacerbation as the primary outcome. Traditional Chinese Medicine syndromes,Modified British Medical Research Council dyspnea scale score, St.George's respiratory questionnaire scores, pulmonary function, and serum inflammatory marker levels [including interleukin-6(IL-6), interleukin-8, tumor necrosis factor-α, and transformation growth factor-β1]were the secondary outcomes.RESULTS: During the 12-week treatment, treatment and control groups had no adverse reactions.The analysis of the indexes obtained from all patients showed that the therapeutic effect in the treatment group was significantly better than that in the control group because most of the similar probabilities of primary and secondary outcomes were less than 0.05,except for the level of IL-6.CONCLUSION: Bufei granule can treat patients with stable COPD by lowering the frequency of acute exacerbation, improving the quality of life,and alleviating the severity of inflammation. | Sijia Guo Zengtao Sun Enshun Liu Jihong Feng Min Fu Yuechuan Li Qi Wu | 2014 | Journal of Traditional Chinese Medicine2014，34，4： | 19 |
| 3 | Cancer incidence in Beijing, 2014显示文摘Objective: To analyze cancer incidence data in Beijing in 2014 and temporal trends for selected common cancers during 2005 and 2014.Methods: A total of 144 secondary and tertiary hospitals reported newly diagnosed cancer cases to Beijing Cancer Registry, which covers 13 million residents in Beijing. The cancer incidence rate was calculated in strata by cancer type, sex, age group and area. The population composition of China in 1982 and Segi's population structure were used to calculate age-standardized rates. Extensive procedures were used to assure the quality of the data.Results: The overall data quality indicators of the percentage of morphology verification(MV)(%), the percentage of death certificate-only(DCO)(%) and the mortality to incidence ratio(M/I) were 72.15%, 0.94% and0.54 respectively. A total of 45,300 new cancer cases were diagnosed in Beijing in 2014. The incidence rate was341.92/100,000(343.50/100,000 in males, 340.33/100,000 in females), and the age-standardized incidence rates by Chinese standard population(ASIRC) and by world standard population(ASIRW) were 143.48/100,000 and182.99/100,000, respectively. The cumulative incidence rate for cancer before 75 years was 20.61%. Cancers of lung, colorectum, liver, stomach and prostate were the top five common cancer types for males, while cancers of breast, lung, thyroid, colorectum and uterus were the top five common cancer types for females. The different patterns were also observed between rural and urban areas. Regarding temporal trends, the incidence of thyroid cancer has the fastest growth between 2005 and 2014. The incidence of liver cancer decreased, and stomach and esophageal cancer also decreased significantly for males in the last decade. Incidence rate for lung cancer was relatively stable during that period of time.Conclusions: With more than 45,000 new cases in Beijing in 2014, cancer remains an important public health problem. Actions should be taken to diminish total cancer incidence in Beijing. | Shuo Liu Lei Yang Yannan Yuan Huichao Li Jing Tian Sijia Lu Ning Wang Jiafu Ji | 2018 | Chinese Journal of Cancer Research2018，30，1： | 11 |
| 4 | Soybean AP1 homologs control flowering time and plant height显示文摘Flowering time and plant height are key agronomic traits that directly affect soybean(Glycine max)yield.APETALA1(AP1)functions as a class A gene in the ABCE model for floral organ development,helping to specify carpel,stamen,petal,and sepal identities.There are four AP1 homologs in soybean,all of which are mainly expressed in the shoot apex.Here,we used clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein 9 technology to generate a homozygous quadruple mutant,gmap1,with loss-of-function mutations in all four GmAP1 genes.Under short-day(SD)conditions,the gmap1 quadruple mutant exhibited delayed flowering,changes in flower morphology,and increased node number and internode length,resulting in plants that were taller than the wild type.Conversely,overexpression of GmAP1a resulted in early flowering and reduced plant height compared to the wild type under SD conditions.The gmap1 mutant and the overexpression lines also exhibited altered expression of several genes related to flowering and gibberellic acid metabolism,thereby providing insight into the role of GmAP1 in the regulatory networks controlling flowering time and plant height in soybean.Increased node number is the trait with the most promise for enhancing soybean pod number and grain yield.Therefore,the mutant alleles of the four AP1 homologs described here will be invaluable for molecular breeding of improved soybean yield. | Liyu Chen Haiyang Nan Lingping Kong Lin Yue Hui Yang Qingsong Zhao Chao Fang Haiyang Li Qun Cheng Sijia Lu Fanjiang Kong Baohui Liu Lidong Dong | 2020 | Journal of Integrative Plant Biology2020，62，12： | 9 |
| 5 | Kinetic hydrate inhibitor performance of new copolymer poly(N-vinyl-2-pyrrolidone-co-2-vinyl pyridine)s with TBAB显示文摘In oil and gas field, the application of kinetic hydrate inhibitors (KHIs) independently has remained problematic in high subcooling and high water-cut situation. One feasible method to resolve this problem is the combined use of KHIs and some synergists, which would enhance KHIs’ inhibitory effect on both hydrate nucleation and hydrate crystal growth. In this study, a novel kind of KHI copolymer poly(N-vinyl-2-pyrrolidone-co-2-vinyl pyridine)s (HGs) is used in conjunction with TBAB to show its high performance on hydrate inhibition. The performance of HGs with different monomer ratios in structure II tetrahydrofuran (THF) hydrate is investigated using kinetic hydrate inhibitor evaluation apparatus by step-cooling method and isothermal cooling method. With the combined gas hydrate inhibitor at the concentration of 1.0 wt%, the induction time of 19 wt% THF solution could be prolonged to 8.5 h at a high subcooling of 6℃. Finally, the mechanism of HGs inhibiting the formation of gas hydrate is proposed. | Jun Hu Sijia Li Yanhong Wang Xuemei Lang Qingping Li Shuanshi Fan | 2012 | Journal of Natural Gas Chemistry2012，21，2： | 8 |
| 6 | Genome-wide variants of Eurasian facial shape differentiation and a prospective model of DNA based face prediction显示文摘It is a long-standing question as to which genes define the characteristic facial features among different ethnic groups. In this study, we use Uyghurs, an ancient admixed population to query the genetic bases why Europeans and Han Chinese look different. Facial traits were analyzed based on high-dense 3D facial images; numerous biometric spaces were examined for divergent facial features between European and Han Chinese, ranging from inter-landmark distances to dense shape geometrics. Genome-wide association studies(GWAS) were conducted on a discovery panel of Uyghurs. Six significant loci were identified, four of which, rs1868752, rs118078182, rs60159418 at or near UBASH3B, COL23A1, PCDH7 and rs17868256 were replicated in independent cohorts of Uyghurs or Southern Han Chinese. A prospective model was also developed to predict 3D faces based on top GWAS signals and tested in hypothetic forensic scenarios. | Lu Qiao Yajun Yang Pengcheng Fu Sile Hu Hang Zhou Shouneng Peng Jingze Tan Yan Lu Haiyi Lou Dongsheng Lu Sijie Wu Jing Guo Li Jin Yaqun Guan Sijia Wang Shuhua Xu Kun Tang | 2018 | Journal of Genetics and Genomics2018，45，8： | 7 |
| 7 | Interlayer-expanded MoS2 assemblies for enhanced electrochemical storage of potassium ions显示文摘Potassium-ion batteries are regarded as the low-cost alternative to lithium-ion batteries.However,their development is hampered by the lack of suitable electrode materials.In this work,we demonstrate that MoS2 with expanded interlayers represents a promising candidate for the electrochemical storage of potassium ions.Hierarchical interlayer-expanded MoS2 assemblies supported on carbon nanotubes are prepared via a straightforward solution method.The increased interlayer spacing not only enables the better accommodation of foreign ions,but also lowers the diffusion energy barrier and improves diffusion kinetics of ions.When investigated as the anode material of potassium ion batteries,our interlayer-expanded MoS2 assemblies exhibit an excellent electrochemical performance with large capacity(up to∼520 mAhg^−1),good rate capability(∼310 mAhg^−1 at 1,000 mAg^−1)and impressive cycling stability,superior to most competitors. | Sijia Di Pan Ding Yeyun Wang Yunling Wu Jun Deng Lin Jia Yanguang Li | 2020 | Nano Research2020，13，1： | 6 |
| 8 | A review:Synthesis,modification and photocatalytic applications of ZnIn_(2)S_(4)显示文摘Solar energy is an ideal energy source for solving energy shortages and serious environmental problems.In the past few decades,photocatalytic technology which uses solar energy to deal with the above problems has caused great interest.ZnIn_(2)S_(4),as a layered ternary metal chalcogenide compound,has a series of advantages such as the wide light absorption range and adjustable bandgap.It has been applied in the different fields of photocatalysis in recent years.This review introduced the crystal structures and growth mechanism of ZnIn_(2)S_(4)and summarized the preparation methods of ZnIn_(2)S_(4).Also,the promoted strategies of ZnIn_(2)S_(4)based photocatalytic system and their applications in the pollutant removal,hydrogen evolution,reduction of CO_(2),nitrogen fixation,and chemical synthesis was summarized.Furthermore,the challenges and development directions of the current ZnIn_(2)S_(4)based photocatalytic system were proposed.It is hoped that this review will help researchers design a better ZnIn_(2)S_(4)based photocatalytic system. | Jie Wang Sijia Sun Run Zhou Yangzi Li Zetian He Hao Ding Daimei Chen Weihua Ao | 2021 | Journal of Materials Science & Technology2021，，19： | 6 |
| 9 | Parallel selection of distinct Tof5 alleles drove the adaptation of cultivated and wild soybean to high latitudes显示文摘Photoperiod responsiveness is a key factor limiting the geographic distribution of cultivated soybean and its wild ancestor.In particular,the genetic basis of the adaptation in wild soybean remains poorly understood.In this study,by combining whole-genome resequencing and genome-wide association studies we identified a novel locus,Time of Flowering 5(Tof5),which promotes flowering and enhances adaptation to high latitudes in both wild and cultivated soybean.By genomic,genetic and transgenic analyses we showed that Tof5 en-codes a homolog of Arabidopsis thaliana FRUITFULL(FUL).Importantly,further analyses suggested that different alleles of Tof5 have undergone parallel selection.The Tof5H1 allele was strongly selected by humans after the early domestication of cultivated soybean,while Tof5H2 allele was naturally selected in wild soybean,and in each case facilitating adaptation to high latitudes.Moreover,we found that the key flowering repressor E1 suppresses the transcription of Tof5 by binding to its promoter.In turn,Tof5 physically associates with the promoters of two important FLOWERING LOCUS T(FT),FT2a and FT5a,to upregulate their transcription and promote flowering under long photoperiods.Collectively,ourfindings provide insights into how wild soybean adapted to high latitudes through natural selection and indicate that cultivated soybean underwent changes in the same gene but evolved a distinct allele that was artificially selected after domestication. | Lidong Dong Qun Cheng Chao Fang Lingping Kong Hui Yang Zhihong Hou Yongli Li Haiyang Nan Yuhang Zhang Qingshan Chen Chunbao Zhang Kun Kou Tong Su Lingshuang Wang Shichen Li Haiyang Li Xiaoya Lin Yang Tang Xiaohui Zhao Sijia Lu Baohui Liu Fanjiang Kong | 2022 | Molecular Plant2022，15，2： | 5 |
| 10 | Double weight determination method for experts of complex multi-attribute large-group decision-making in interval-valued intuitionistic fuzzy environment显示文摘The systematic clustering analysis based weight determination methods are suitable for the experts of complex multiattribute large-group decision-making(CMALGDM) in intervalvalued intuitionistic fuzzy environment. However, these methods mainly have two shortcomings: they do not consider the consistency of the experts in each aggregation; the aggregation weights are often determined simply by the 'majority principle', and neglect the quantity of information provided by the holistic aggregation,leading to decision biases. Hence, a double weight determination method for experts is proposed to solve these problems. As for the first shortcoming, a mathematical programming model is used to solve for the optimal expert weights within each aggregation,ensuring consistency in the overall preferences of the aggregations. As for the second shortcoming, we propose a modification of the aggregation weights based on the information entropy, which fully considers both the number of experts and the amount of information provided by the holistic aggregation. With the proposed method, the final expert weights are determined more rigorously and objectively. The feasibility of the proposed method is investigated through an illustrative example. | Bingsheng Liu Sijia Guo Kaijing Yan Ling Li Xueqing Wang | 2017 | Journal of Systems Engineering and Electronics2017，28，1： | 4 |
| 11 | A rapid, automated flaw segmentation method using morphological reconstruction to grade wood flooring显示文摘Region-Growing Algorithms(RGAs) are used to grade the quality of manufactured wood flooring. Traditional RGAs are hampered by problems of long segmentation time and low inspection accuracy caused by neighborhood search. We used morphological reconstruction with the R component to construct a novel flaw segmentation method. We initially designed two template images for low and high thresholds, and these were used for seed optimization and inflation growth, respectively. Then the extraction of the flaw skeleton from the low threshold image was realized by applying the erosion termination rules. The seeds in the flaw skeleton were optimized by the pruning method. The geodesic inflection was applied by the high threshold template to realize rapid growth of the flaw area in the floor plate, and region filling and pruning operations were applied for margin optimization. Experiments were conducted on 512×512, 256×256 and 128×128 pixel sizes, respectively. The 256×256 pixel size proved superior in time-consumption at 0.06 s with accuracy of 100%. But with the region-growing method the same process took 0.22 s with accuracy of 70%. Compared with RGA, our proposed method can realize more accurate segmentation, and the speed and accuracy of segmentation can satisfy the requirements for on-line grading of wood flooring. | Yizhuo Zhang Sijia Liu Jun Cao Chao Li Huiling Yu | 2014 | Journal of Forestry Research2014，25，4： | 3 |
| 12 | Characterization of Electric Field Distribution Within High Voltage Press-packed IGBT Submodules Under Conditions of Repetitive Turn-on and Turn-off显示文摘The high-voltage high-power press-packed IGBT(PPI)devices are the key component of the DC transmission apparatus.A PPI device is composed of several PPI submodules.In general,the PPI submodule works in a state of repetitive turn-on and turn-off,and the corresponding working voltage is the positive periodic square waveform(PPSW)voltage,which is much different from the conventional AC or DC voltage.In addition,insulation capability is one of the most critical challenges in the design and fabrication of PPI devices.To improve the insulation capability of the device,composite insulation structures with multiple dielectrics are usually employed.Under the PPSW voltage,it is essential to analyze the transient electric field to solve the insulation challenge of the PPI devices.However,the electric field of the PPI is often calculated under the electrostatic field or DC field.Moreover,the transient characteristics of the electric field are ignored.Therefore,this paper focuses on the analysis of the transient characteristics of the electric field of the PPI submodule under the PPSW voltage.The influences of the waveform parameters of the PPSW voltage on the transient characteristics are demonstrated in detail.This study is significant for the insulation analysis and design of the PPIs. | Teng Wen Xiang Cui Sijia Liu Xuebao Li Zhibin Zhao | 2022 | CSEE Journal of Power and Energy Systems2022，8，2： | 3 |
| 13 | Brain-wide Mapping of Mono-synaptic Afferents to Different Cell Types in the Laterodorsal Tegmentum显示文摘The laterodorsal tegmentum(LDT) is a brain structure involved in distinct behaviors including arousal,reward, and innate fear. How environmental stimuli and top-down control from high-order sensory and limbic cortical areas converge and coordinate in this region to modulate diverse behavioral outputs remains unclear.Using a modified rabies virus, we applied monosynaptic retrograde tracing to the whole brain to examine the LDT cell type specific upstream nuclei. The LDT received very strong midbrain and hindbrain afferents and moderate cortical and hypothalamic innervation but weak connections to the thalamus. The main projection neurons from cortical areas were restricted to the limbic lobe, including the ventral orbital cortex(VO), prelimbic, and cingulate cortices. Although different cell populations received qualitatively similar inputs, primarily via afferents from the periaqueductal gray area, superior colliculus, and the LDT itself, parvalbumin-positive(PV?) GABAergic cells received preferential projections from local LDT neurons.With regard to the different subtypes of GABAergic cells, aconsiderable number of nuclei, including those of the ventral tegmental area, central amygdaloid nucleus, and VO, made significantly greater inputs to somatostatinpositive cells than to PV?cells. Diverse inputs to the LDT on a system-wide level were revealed. | Xiaomeng Wang Hongbin Yang Libiao Pan Sijia Hao Xiaotong Wu Li Zhan Yijun Liu Fan Meng Huifang Lou Ying Shen Shumin Duan Hao Wang | 2019 | Neuroscience Bulletin2019，35，5： | 3 |
| 14 | New ZnCe catalyst encapsulated in SBA-15 in the production of 1,3-butadiene from ethanol显示文摘ZnO-CeO2/SBA-15 catalysts were prepared by two kinds of solid-state grinding method and used for the production of 1,3-butadiene(1,3-BD) from ethanol.A mixture of SBA-15(with or without organic template) and metal precursors were ground in solid-state.The obtained catalysts were characterized by TG,N2 adsorption-desorption,TEM,XRD,Py-FTIR and NH_3-TPD techniques.Superior dispersion of metal oxides and more exposed acid sites were achieved on the catalyst lOZn_1Ce_5-AS with the presence of organic template in SBA-15 during the solid-state grinding process.The catalytic performance was evaluated in a fixed-bed reactor and a 1,3-butadiene selectivity of as high as 45% is achieved.This is attributed to the coupling effect of Zn and Ce species in the mesopores of SBA-15,in which Zn promotes ethanol dehydrogenation and Ce enhances aldol-condensation,respectively.Additionally,solvent-free method inspires new catalyst synthesis strategy for the production of 1,3-butadiene from ethanol. | Yujun Zhao Sijia Li Zheng Wang Shengnian Wang Shengping Wang Xinbin Ma | 2020 | Chinese Chemical Letters2020，31，2： | 3 |
| 15 | Targeting a novel inducible GPX4 alternative isoform to alleviate ferroptosis and treat metabolic-associated fatty liver disease显示文摘Metabolic-associated fatty liver disease(MAFLD),which is previously known as non-alcoholic fatty liver disease(NAFLD),represents a major health concern worldwide with limited therapy.Here,we provide evidence that ferroptosis,a novel form of regulated cell death characterized by iron-driven lipid peroxidation,was comprehensively activated in liver tissues from MAFLD patients.The canonical-GPX4(cGPX4),which is the most important negative controller of ferroptosis,is downregulated at protein but not mRNA level.Interestingly,a non-canonical GPX4 transcript-variant is induced(inducible-GPX4,iGPX4)in MAFLD condition.The high fat-fructose/sucrose diet(HFFD)and methionine/choline-deficient diet(MCD)-induced MAFLD pathologies,including hepatocellular ballooning,steatohepatitis andfibrosis,were attenuated and aggravated,respectively,in cGPX4-and iGPX4-knockin mice.cGPX4 and iGPX4 isoforms also displayed opposing effects on oxidative stress and ferroptosis in hepatocytes.Knockdown of iGPX4 by siRNA alleviated lipid stress,ferroptosis and cell injury.Mechanistically,the triggered iGPX4 interacts with cGPX4 to facilitate the transformation of cGPX4 from enzymatic-active monomer to enzymatic-inactive oligomers upon lipid stress,and thus promotes ferroptosis.Co-immunoprecipitation and nano LC–MS/MS analyses confirmed the interaction between iGPX4 and cGPX4.Our results reveal a detrimental role of non-canonical GPX4 isoform in ferroptosis,and indicate selectively targeting iGPX4 may be a promising therapeutic strategy for MAFLD. | Jie Tong Dongjie Li Hongbo Meng Diyang Sun Xiuting Lan Min Ni Jiawei Ma Feiyan Zeng Sijia Sun Jiangtao Fu Guoqiang Li Qingxin Ji Guoyan Zhang Qirui Shen Yuanyuan Wang Jiahui Zhu Yi Zhao Xujie Wang Yi Liu Shenxi Ouyang Chunquan Sheng Fuming Shen Pei Wang | 2022 | Acta Pharmaceutica Sinica B2022，12，9： | 3 |
| 16 | In-situ observation of electrical tree evolution in epoxy dielectrics with internal cracks显示文摘Gas-filled internal crack might appear in thermoset materials like epoxy resin during the equipment manufacturing,which would become a vulnerable local region to initiate the electrical tree,thus prone to cause insulation failure.The withstand voltage test was carried on epoxy samples with artificial cracks based on a rod-plane electrode arrange-ment.Simultaneously,surface state variation and tree evolution with crack were observed by an optical microscope in conjunction with a charge-coupled device camera.The changes in morphology and chemical status of the crack surface were characterized by scanning electron microscopy,laser Raman spectrometer and energy dispersive spec-trometer,respectively.It was found that the erosion and tree started from the borderline of crack under a relatively low electric field strength;however,the area near the electrode had relatively little damage.The breaking of epoxy molecular chains coarsens the crack surface and further forms deep channels on a micro-level,which is the forerunner of the electrical tree inception.Based on these,the initiation mechanisms of the crack-induced electrical tree and the reasons for the erosion near the borderline have been revealed.This study provides a train thought for the polymer degradation opening up into the initial tree channel during tree evolution processes. | Zehua Wu Huidong Tian Sijia Zhu He Li Qingyu Wang Zongliang Xie Peng Liu Zongren Peng | 2021 | High Voltage2021，6，2： | 3 |
| 17 | Two soybean homologues of TERMINAL FLOWER 1 control flowering time under long day conditions显示文摘Flowering time is a key agronomic trait that directly affect the adaptation and yield of soybean.After whole genome duplications,about 75%of genes being represented by multiple copies in soybean.There are four TERMINAL FLOWER 1(TFL1)genes in soybean,and the TFL1b(Dt1)has been characterized as the determinant of stem growth habit.The function of other TFL1 homologs in soybean is still unclear.Here,we generated knockout mutants by CRISPR/Cas9 genome editing technology and found that the tfl1c/tfl1d double mutants flowered significantly earlier than wild-type plants.We investigated that TFL1c and TFL1d could physically interact with the b ZIP transcription factor FDc1 and bind to the promoter of APETALA1a(AP1a).RNA-seq and q RT-PCR analyses indicated that TFL1c and TFL1d repressed the expressions of the four AP1 homologs and delayed the flowering time in soybean.The two genes play important roles in the regulation of flowering time in soybean and mainly act as the flowering inhibitors under long-day conditions.Our results identify novel components in the flowering-time regulation network of soybean and will be invaluable for molecular breeding of improved soybean yield. | Lingshuang Wang Chun Lin Bohui Li Tong Su Shichen Li Haiyang Li Fanglei He Chuanjie Gou Zheng Chen Yanan Wang Jun Qin Baohui Liu Fanjiang Kong Lin Yue Sijia Lu Chao Fang | 2023 | The Crop Journal2023，11，3： | 3 |
| 18 | Effects of Qizhukangxian granules on idiopathic pulmonary fibrosis:a randomized,double blind,placebo-controlled and multicenter clinical pilot trial显示文摘OBJECTIVE:To evaluate the effects of Qizhukangxian granules(QG)on idiopathic pulmonary fibrosis(IPF).METHODS:This is a randomized,double blind,placebo-controlled and multicenter clinical pilot trial.Six medical centers in Tianjin,China,participated in the study.A total of 120 IPF patients were enrolled and randomized into two groups,with 60 patients in each group.The treatment group was treated with QG,while the control group received a Qizhukangxian placebo.The pharmacological treatment lasted for 48 weeks from the enrollment date.The indexes of patients were recorded on the admission day and at the end of the 24th and 48th weeks.Data were analyzed to study the effects of QG;forced vital capacity,change in forced vital capacity and maximal 6-min walk test(6MWT)distance were the primary endpoints.Secondary endpoints were percentage of patients with episodes of acute exacerbation of IPF,pulmonary function,changes in pulse oxygen saturation during the 6MWT,dyspnea score,St.George's respiratory questionnaire score,arterial blood gas analyses and the total Traditional Chinese Medicine symptom pattern score.RESULTS:After 24 weeks of treatment,QG showed greater efficacy than the placebo in certain parameters,including the dyspnea score,Traditional Chinese Medicine symptom pattern score and some indicators in the St.George's respiratory questionnaire score.Analysis of the indexes obtained from all patients at the end of the 48th week showed that the therapeutic effects in the treatment group were significantly better than those in the control group because remarkable differences were observed in most of the primary and secondary endpoints between the two groups,except for the maximal distance of the 6MWT and arterial blood gas analyses.No adverse reaction was observed in either group during the 48-week trial treatment period.CONCLUSION:QG could effectively treat IPF patients by ameliorating pulmonary function,improving the quality of life and lowering the percentage of acute exacerbations. | Guo Sijia Song Yalin Feng Jihong Liu Shuang Li Yuechuan Liu Min Wei Luqing Zhang Xian Xie Hui Sun Zengtao | 2020 | Journal of Traditional Chinese Medicine2020，40，4： | 3 |
| 19 | Osteogenesis effects of magnetic nanoparticles modified-porous scaffolds for the reconstruction of bone defect after bone tumor resection显示文摘The treatment of bone defect after bone tumor resection is a great challenge for orthopedic surgeons.It should consider that not only to inhibit tumor growth and recurrence,but also to repair the defect and preserve the limb function.Hence,it is necessary to find an ideal functional biomaterial that can repair bone defects and inactivate tumor.Magnetic nanoparticles(MNPs)have its unique advantages to achieve targeted hyperthermia to avoid damage to surrounding normal tissues and promote osteoblastic activity and bone formation.Based on the previous stage,we successfully prepared hydroxyapatite(HAP)composite poly(lactic-co-glycolic acid)(PLGA)scaffolds and verified its good osteogenic properties,in this study,we produced an HAP composite PLGA scaffolds modified with MNPs.The composite scaffold showed appropriate porosity and mechanical characteristics,while MNPs possessed excellent magnetic and thermal properties.The cytological assay indicated that the MNPs have antitumor ability and the composite scaffold possessed good biocompatibility.In vivo bone defect repair experiment revealed that the composite scaffold had good osteogenic capacity.Hence,we could demonstrate that the composite scaffolds have a good effect in bone repair,which could provide a potential approach for repairing bone defect after bone tumor excision. | Ming Li Jianheng Liu Xiang Cui Guofei Sun Jianwei Hu Sijia Xu Fei Yang Licheng Zhang XiumeiWang Peifu Tang | 2019 | Regenerative Biomaterials2019，6，6： | 3 |
| 20 | Biological evaluation and interaction mechanism of beta-site APP cleaving enzyme 1 inhibitory pentapeptide from egg albumin显示文摘Inhibition of beta-site APP cleaving enzyme1(BACE1)is one of the most promising therapeutic approaches for Alzheimer’s disease.To find natural products for the treatment of Alzheimer’s disease,absorption,distribution,metabolism,excretion and toxicity(ADMET)properties and in vitro BACE1 inhibitory activity of the peptides isolated from egg albumin were evaluated.Then,molecular docking and molecular dynamics simulation were used to explain the molecular mechanism of the interactions between BACE1 and peptides.The IC50 value of peptide KLPGF,with satisfactory ADMET properties,against BACE1 was(8.30±0.56)mmol/L.Molecular docking revealed that KLPGF contacted with the residues of BACE1’s active sites through twelve hydrogen bonds interactions,two hydrophobic interactions,one electrostatic interaction,and two Pi-cation interactions.The 5 ns molecular dynamics simulations confirmed that the structure of KLPGF with BACE1 was stable.Peptide KLPGF contacted the residues Lys321,Asp228,and Asn233 with stable hydrogen bonds.KLPGF may be a potential anti-BACE1 candidate. | Zhipeng Yu Sijia Wu Wenzhu Zhao Long Ding David Shiuan Fuping Zheng Jianrong Li Jingbo Liu | 2020 | Food Science and Human Wellness2020，9，2： | 2 |
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