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| 1 | Rice Expression Database(RED):An integrated RNA-Seq-derived gene expression database for rice显示文摘Rice is one of the most important stable food as well as a monocotyledonous model organism for the plant research community.Here,we present RED(Rice Expression Database;http://gffzzd1bc3ab4bbbc4005h0pc56vkxxon56qcw.ffgz.tsg.suse.edu.cn),an integrated database of rice gene expression profiles derived entirely from RNA-Seq data.RED features a comprehensive collection of 284 high-quality RNA-Seq experiments,integrates a large number of gene expression profiles and covers a wide range of rice growth stages as well as various treatments.Based on massive expression profiles,RED provides a list of housekeeping and tissue-specific genes and dynamically constructs co-expression networks for gene(s) of interest.Besides,it provides user-friendly web interfaces for querying,browsing and visualizing expression profiles of concerned genes.Together,as a core resource in BIG Data Center,RED bears great utility for characterizing the function of rice genes and better understanding important biological processes and mechanisms underlying complex agronomic traits in rice. | Lin Xia Dong Zou Jian Sang Xingjian Xu Hongyan Yin Mengwei Li Shuangyang Wu Songnian Hu Lili Hao Zhang Zhang | 2017 | Journal of Genetics and Genomics2017，44，5： | 12 |
| 2 | The apricot(Prunus armeniaca L.)genome elucidates Rosaceae evolution and beta-carotenoid synthesis显示文摘Apricots,scientifically known as Prunus armeniaca L,are drupes that resemble and are closely related to peaches or plums.As one of the top consumed fruits,apricots are widely grown worldwide except in Antarctica.A high-quality reference genome for apricot is still unavailable,which has become a handicap that has dramatically limited the elucidation of the associations of phenotypes with the genetic background,evolutionary diversity,and population diversity in apricot.DNA from P.armeniaca was used to generate a standard,size-selected library with an average DNA fragment size of~20 kb.The library was run on Sequel SMRT Cells,generating a total of 16.54 Gb of PacBio subreads(N50=13.55 kb).The high-quality P.armeniaca reference genome presented here was assembled using long-read single-molecule sequencing at approximately 70×coverage and 171×Illumina reads(40.46 Gb),combined with a genetic map for chromosome scaffolding.The assembled genome size was 221.9 Mb,with a contig NG50 size of 1.02 Mb.Scaffolds covering 92.88%of the assembled genome were anchored on eight chromosomes.Benchmarking Universal Single-Copy Orthologs analysis showed 98.0%complete genes.We predicted 30,436 protein-coding genes,and 38.28%of the genome was predicted to be repetitive.We found 981 contracted gene families,1324 expanded gene families and 2300 apricot-specific genes.The differentially expressed gene(DEG)analysis indicated that a change in the expression of the 9-cis-epoxycarotenoid dioxygenase(NCED)gene but not lycopene beta-cyclase(LcyB)gene results in a lowβ-carotenoid content in the white cultivar“Dabaixing”.This complete and highly contiguous P.armeniaca reference genome will be of help for future studies of resistance to plum pox virus(PPV)and the identification and characterization of important agronomic genes and breeding strategies in apricot. | Fengchao Jiang Junhuan Zhang Sen Wang Li Yang Yingfeng Luo Shenghan Gao Meiling Zhang Shuangyang Wu Songnian Hu Haoyuan Sun Yuzhu Wang | 2019 | Horticulture Research2019，6，1： | 9 |
| 3 | Laboratory test on the combined cooling effect of L-shaped thermosyphons and thermal insulation on high-grade roadway construction in permafrost regions显示文摘With the completion of the Qinghai-Tibetan Railway,economic development of related areas has been greatly accelerated.This,in return,calls for building or upgrading more roadways,especially high-grade roadways.In cold regions,the thawing of permafrost can induce settlement damage of and even failure to railway (or roadway) embankments.Thermosyphons (self-powered refrigera-tion devices that are used to help keep the permafrost cool) have proved effective in mitigating thaw settlement by maintaining the thermal stability of the embankments.However,for high-grade roadway embankments of great width,stabilizing or cooling ef-fects of traditional geotechnological measures may be limited.To enhance the cooling effect of thermosyphons,an L-shaped thermosyphon was designed.A laboratory test was carried out to study the combined cooling effect of the L-shaped thermosyphon and thermal insulation applying to roadbed construction.The angle between the evaporator and condenser sections of the L-shaped thermosyphon is 134 degrees,and the L-shaped thermosyphon was inserted into the soil at an angle of 5 degrees with the road surface.The tested results show that the L-shaped thermosyphon is effective in removing heat from a roadway in winter.When the ambient air temperature is lower than the soil temperature,the thermosyphon is active and extracts the heat in the soil around it.When the ambient air temperature is higher than the soil temperature,the thermosyphon is inactive,and no heat is in-jected into the soil through the L-shaped thermosyphon.Compared to embankments with straight thermosyphons,the inner parts of the embankments with L-shaped thermosyphons were significantly cooled.It is hoped that the present study would be useful to the application of L-shaped thermosyphons in the construction of high-grade roadways in cold regions. | YuanHong Dong,YuanMing Lai,MingYi Zhang,ShuangYang Li State Key Laboratory of Frozen Soil Engineering,Cold and Arid Regions Environmental and Engineering Research Institute,Chinese Academy of Sciences,Lanzhou,Gansu 730000,China. | 2009 | Research in Cold and Arid Regions2009，1，4： | 3 |
| 4 | Numerical study on the influence of geometrical parameters on natural convection cooling effect of the crushed-rock revetment显示文摘The crushed-rock revetment, considered as a highly porous medium, is often applied to embankment slope to protect the underlying permafrost and ensure the thermal stability of roadway in permafrost regions. In this paper, in order to increase and optimize the cooling capacity of crushed-rock revetment in cold regions roadway engineering, based on the thermal convection theories, the practical geometry and the temperature characteristics of in-situ crushed-rock revetment, convective pattern and cooling effect of crushed-rock revetment are numerically studied, with a thickness of 1.0 m under different geometrical parameters, e.g. sloped angle and aspect ratio. The results indicate that, with a thickness of 1.0 m and a temperature difference of 10.0 ℃ between top and bottom boundaries, due to the existence of natural convection, the effective thermal conductivity of crushed-rock revetment can be increased and the thermal 'semi-conductor' characteristics are endowed. However, the convective pattern changes with the variation of the sloped angle, namely, with the increase of the sloped angle, the convection cell number decreases; furthermore, when the flow changes from various cells to a single cell at a sloped angle, the Nu number is the smallest and the cooling effect is the worst, therefore, the corresponding sloped angle is considered as the worst cooling sloped angle. Besides, with the increase of the aspect ratio, the worst cooling sloped angle increases and tends to 32°, also approaching to the in-situ crushed-rock revetment angle 33.7°. Therefore, when the crushed-rock revetment em-bankment is too high, that is to say, the aspect ratio of the sloped crushed-rock revetment is too large, some measures should be taken to enhance its cooling effect, which have been researched and discussed in this paper. It is hoped that some scientific references can be supplied to the design and maintenance of the crushed-rock revetment embankment in cold regions. | ZHANG MingYi CHENG GuoDong LI ShuangYang | 2009 | Science China(Technological Sciences)2009，52，2： | 2 |
| 5 | Simultaneous determination of 5-nitroimidazoles and nitrofurans in pork by high-performance liquid chromatography–tandem mass spectrometry显示文摘 | Xi Xia Xiaowei Li Suxia Zhang Shuangyang Ding Haiyang Jiang Jiancheng Li Jianzhong Shen | 2008 | Journal of Chromatography A2008，，1： | 2 |
| 6 | Genomic Insight into the Antimicrobial Resistance of Streptococcus Suis-Six Countries,2011-2019显示文摘Summary What is already known on this topic?Streptococcus suis(S.suis)is a zoonotic pathogen causing disease in humans and animals,and the emergence of its increased resistance to antimicrobial agents has become a significant challenge in many countries. | Lichao Ma Wanjiang Zhang Jingyun Ma Qingpo Cui Chaoyang Zhang Peng Zhang Chengtao Sun Huarun Sun Yao Zhu Shaolin Wang Shuangyang Ding Gongzheng Hu Zhangqi Shen | 2021 | China CDC weekly2021，3，47： | 2 |
| 7 | New Delay Doppler Communication Paradigm in 6G Era:A Survey of Orthogonal Time Frequency Space(OTFS)显示文摘In the 6G era,Space-Air-Ground Integrated Network(SAGIN)are anticipated to deliver global coverage,necessitating support for a diverse array of emerging applications in high-mobility,hostile environments.Under such conditions,conventional orthogonal frequency division multiplexing(OFDM)modulation,widely employed in cellular and Wi-Fi communication systems,experiences performance degradation due to significant Doppler shifts.To overcome this obstacle,a novel twodimensional(2D)modulation approach,namely orthogonal time frequency space(OTFS),has emerged as a key enabler for future high-mobility use cases.Distinctively,OTFS modulates information within the delay-Doppler(DD)domain,as opposed to the timefrequency(TF)domain utilized by OFDM.This offers advantages such as Doppler and delay resilience,reduced signaling latency,a lower peak-to-average ratio(PAPR),and a reduced-complexity implementation.Recent studies further indicate that the direct interplay between information and the physical world in the DD domain positions OTFS as a promising waveform for achieving integrated sensing and communications(ISAC).In this article,we present an in-depth review of OTFS technology in the context of the 6G era,encompassing fundamentals,recent advancements,and future directions.Our objective is to provide a helpful resource for researchers engaged in the field of OTFS. | Weijie Yuan Shuangyang Li Zhiqiang Wei Yuanhao Cui Jiamo Jiang Haijun Zhang Pingzhi Fan | 2023 | China Communications2023，20，6： | 2 |
| 8 | Molecular Epidemiology of Klebsiella pneumoniae from Clinical Bovine Mastitis in Northern Area of China,2018–2019显示文摘Klebsiella pneumoniae(K.pneumonia,KpⅠ)is a predominate inducement of bovine mastitis,which is associated with high mortality and milk yield reduction.However,data is lacking on the molecular characteristics of bovine K.pneumoniae,limiting the risk assessment of its transmission through the food chain.Herein,we investigated the prevalence of K.pneumoniae in 6301 clinical mastitis(CM)milk samples from dairy cattle in northern area of China.In total,183 K.pneumoniae isolates were recovered,with detection rates of 3.0% and 2.8% in 2018 and 2019,respectively.Like human clinical K.pneumoniae,all CM K.pneumoniae isolates belonged to one of three phylogroups:KpⅠ(n=143),Klebsiella.quasipneumoniae subsp.similipneumoniae(KpⅡ-B)(n=37),and Klebsiella variicola(KpⅢ)(n=3).We detected the extendedspectrum β-lactamase-encoding genes bla_(SHV-2a),blac_(CTX-M-14),and bla_(CTX-M-15),as well as clpC,lpfA,lacI,lacZ,lacY,and the fecABDEIR operon in the KpⅠ isolates,which may contribute to their pathogenicity and host adaptability in cows.The high prevalence of KpⅠ in dairy farms may be problematic,as it showed relatively higher rates of antibiotic resistance and virulence gene carriage than the KpⅡ-B and KpⅢ isolates.Furthermore,we observed distinct differences in population structure between CM-and human infection-associated KpⅠ isolates,with the genes associated with invasive infection in humans rarely being observed in bovine isolates,indicating that few CM-associated K.pneumoniae isolates pose a threat to human health.Nevertheless,bovine KpⅡ-B isolates shared a high level of nucleotide sequence identity with isolates from human infections and frequently carried the nitrogen-fixation gene nif,suggesting an association between KpⅡ-B isolates from cattle and humans,and plant-derived bacteria. | Shikai Song Wenjuan He Dawei Yang Manar Benmouffok Yao Wang Jiyun Li Chengtao Sun Xiangbin Song Shizhen Ma Chang Cai Shuangyang Ding Congming Wu Zhangqi Shen Yang Wang | 2022 | Engineering2022，8，3： | 1 |
| 9 | A novel quantum dot-based fluoroimmunoassay method for detection of Enrofloxacin residue in chicken muscle tissue显示文摘 | Junxia Chen Fei Xu Haiyang Jiang Yali Hou Qinxiong Rao Pengju Guo Shuangyang Ding | 2008 | Food Chemistry2008，，4： | 1 |
| 10 | Strength distributions of warm frozen clay and its stochastic damage constitutive model显示文摘 | LAI YUANMING LI SHUANGYANG QI JILIN | 2008 | Cold Regions Science and Technology2008，53，2： | 1 |
| 11 | Experimental Simulation and Verification of Position Servo Control of Mechanical Rodless Cylinder显示文摘In order to improve the position control accuracy of rodless cylinder,the valve control cylinder system based on pneumatic proportional servo is studied deeply.According to the working principle of the mechanical rodless cylinder control system,under the condition of uniform speed,the driving voltage of the proportional valve is changed to measure multiple sets of friction force and corresponding velocity data.Analyzed the physical structure of each component in pneumatic system,established the mathematical model of pneumatic system,and introduced MATLAB system identification toolbox to identify the parameters of the transfer function.and the experiment verifies its correctness. | Yeming ZHANG Kaimin LI Hongwei YUE Shuangyang HE Dongyuan LI Kun LYU Feng WEI | 2020 | Mechanical Engineering Science2020，2，2： | 1 |
| 12 | Simultaneous analysis of avermectins in bovine tissues by LC-MS-MS with immunoaffnity chromatography显示文摘 | HOU Xiaolin LI Xiaowei DING Shuangyang | 2006 | Chromatographia2006，63，1112： | 1 |
| 13 | Minimum ground pre-freezing time before excavation of Guangzhou subway tunnel显示文摘 | Shuangyang Li Yuanming Lai Mingyi Zhang | 2006 | Cold Regions Science and Technology2006，46，： | 1 |
| 14 | Development of a monoclonal antibody-based broad-specificity ELISA for fluoroquinolone antibiotics in foods and molecular modeling studies of cross-reactive compounds显示文摘 | WANG Zhanhui ZHU Yan DING Shuangyang | 2007 | Analytical Chemistry2007，79，： | 1 |
| 15 | An Improved Statistical Damage Constitutive Model forWarm Frozen Clay Based on Mohr-Coulomb Criterion 显示文摘 | LI Shuangyang LAI Yuanming ZHANG Shujuan | 2008 | Cold Regions Science and Technology2008，57，23： | 1 |
| 16 | Study on Long-term Stability of Qinghai-Tibet Railway Embankment 显示文摘 | LI Shuangyang LAI Yuanming ZHANG Mingyi | 2009 | Cold Regions Science and Technology2009，57，23： | 1 |
| 17 | Lower urinary tract symptoms in women with irritable bowel syndrome显示文摘 | Ya‐JunGuo Chen‐HsunHo Shyh‐ChyanChen Shun‐ShuangYang Han‐MoChiu Kuo‐HowHuang | 2010 | International Journal of Urology2010，，2： | 1 |
| 18 | Characterization of a Novel Gene, srpA, Conferring Resistance to Streptogramin A, Pleuromutilins, and Lincosamides in Streptococcus suis显示文摘Antimicrobial resistance is undoubtedly one of the greatest global health threats. The emergence of multidrug-resistant(MDR) Gram-positive pathogens, such as methicillin-resistant Staphylococcus aureus(MRSA), vancomycin-resistant Enterococcus faecium(VRE), and β-lactamase-resistant Streptococcus pneumonia, has severely limited our antibiotic arsenal. Numerous ribosome-targeting antibiotics, especially pleuromutilins, oxazolidinones, and streptogramins, are viewed as promising alternatives against aggressive MDR pathogens. In this study, we identified a new adenosine triphosphate(ATP)-binding cassete(ABC)-F family determinant, srp A, in Streptococcus suis(S. suis) by means of a comparative analysis of the whole-genome sequences of tiamulin(TIA)-resistant and TIA-sensitive bacteria. Functional cloning confirmed that the deduced gene can mediate cross-resistance to pleuromutilins, lincosamides, and streptogramin A in S. suis and S. aureus. A sequence alignment revealed that Srp A shares the highest amino acid identity with Vga(E)(36%) and shows canonical characteristics of ABC-F family members.In Srp A-ribosome docked compounds, the extended loop region of Srp A approaches the valnemulinbinding pocket in the ribosome peptidyl-transferase center and competes with bound valnemulin. A detailed mutational analysis of the loop residues confirmed that this domain is crucial for Srp A activity,as substitutions or truncations of this region affect the efficiency and specificity of antibiotic resistance.Intracellular antibiotics accumulation indicated that Srp A does not act as an efflux pump, while a ribosome binding assay supported the protective effects of Srp A on the ribosome by preventing antibiotic binding as well as displacing bound drugs. These findings clarify the mechanisms underlying resistance to ribosomal antibiotics. | Chaoyang Zhang Lu Liu Peng Zhang Jingpo Cui Xiaoxia Qin Lichao Ma Kun Han Zhanhui Wang Shaolin Wang Shuangyang Ding Zhangqi Shen | 2022 | Engineering2022，8，2： | 1 |
| 19 | Minimum ground pre -freezing time before excavation of Guangzhou subway tunnel 显示文摘 | LI Shuangyang LAI Yuanming ZHANG Mingyi | 2006 | Cold Regions Science and Technology2006，46，9： | 1 |
| 20 | Development of a monoclonal antibody-based broad-specificity ELISA for fluoroquinolone antibiotics in foods and molecular modeling studies of cross-reactive compounds显示文摘 | WANG Zhanhui ZHU Yah DING Shuangyang ct al | 2007 | Anal Chem2007，79，12： | 1 |
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