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| 1 | Experimental Study on Preparation of Natural Gas Hydrate by Crystallization显示文摘In this paper, the saturated solution crystallization method is proposed to promote the formation of hydrate by means of the known similarities between the hydrate formation process and the crystallization process. In this method,adding the second phase crystals was used to replace the spontaneous formation of hydrate crystal nuclei to form hydrate.The effects of saturated Na_2SO_4, MgSO_4, NH_4HCO_3 and CuSO_4 solutions on the formation rates of natural gas hydrate and gas storage capacity were investigated. The results showed that the saturated solution had an influence on the hydrate formation process. Under the given experimental conditions, the saturated Na_2SO_4 solution showed a highest increase in the hydrate formation rate, and the average hydrate formation rate in its presence was 11.8 times higher than that obtained in the deionized water. Moreover, the largest formation rate of gas hydrates observed in the saturated Na_2SO_4 solution was 386 times bigger than that in the deionized water, and the gas storage capacity increased by 10 times. In addition, the average hydrate formation rate in the saturated Mg SO_4 solution was faster than that in water by 20 times. The largest formation rate of gas hydrates in the saturated MgSO_4 solution was 165 times faster than that obtained in the deionized water, and the gas storage capacity increased by 6.2 times. The saturated NH_4HCO_3 and saturated CuSO_4 solutions also influenced the formation process of hydrate. Therefore, the crystallization method of saturated solution can be used to achieve a highefficiency preparation of natural gas hydrates, which provides theoretical guidance for the storage of natural gas in the form of hydrate. | Ma Shihui Pan Zhen Li Ping Wu Yuguo Li Bingfan Kang Jinke Zhang Zhien | 2017 | China Petroleum Processing & Petrochemical Technology2017，19，1： | 7 |
| 2 | RuCl_3 anchored onto post-synthetic modification MIL-101(Cr)-NH_2 as heterogeneous catalyst for hydrogenation of CO_2 to formic acid显示文摘A series of efficient ruthenium chloride (RuCl_3)-anchored MOF catalysts,such as RuCl_3@MIL-101 (Cr)-Sal,and RuCl_3@MIL-101 (Cr)-DPPB, have been successfully synthesized by post-synthetic modification (PSM)of the terminal amino of MIL-101(Cr)-NH_2 with salicylaldehyde, 2-diphenylphosphinobenzaldehyde (DPPBde) and anchoring of Ru (Ⅲ) ions. The stronger coordination electron donor interaction between Ru (Ⅲ) ions and chelating groups in the RuCl_3@MIL-101 (Cr)-DPPB enhances its catalytic performance for CO_2 hydrogenation to formic acid. The turnover number (TON) of formic acid was up to 831 in reaction time of 2 h with dimethyl sulfoxide (DMSO) and water (H_2O) as mixed solvent, trimethylamine (Et_3N) as organic base, and PPh_3 as electronic additive. | Shengping Wang Shihui Hou Chao Wu Yujun Zhao Xinbin Ma | 2019 | Chinese Chemical Letters2019，30，2： | 5 |
| 3 | Asymmetric neurocognitive representation of ethnic in-group/out-group faces显示文摘To investigate asymmetric neurocognitive representation of ethnic in-group and out-group members, we recorded event-related potentials (ERPs) to faces in a perceptual task after the faces had been primed with positive or negative affective links. The affective link priming did not influence the ERPs to ethnic out-group faces. However, relative to the positive affective link priming, the negative affective link priming increased the amplitudes of an early frontal negativity (N100) and a following central negativity but decreased the amplitude of a late positive potential elicited by ethnic in-group faces. Moreover, the N100 amplitude correlated with the degree of negative attitudes towards ethnic in-group faces. The findings suggest that multiple-level neural mechanisms are involved in individuation of heterogeneous ethnic in-group faces. | MA YiNa GE JianQiao XU XiaoJing FAN Yan YANG ShengMin HAN ShiHui | 2009 | Chinese Science Bulletin2009，54，12： | 3 |
| 4 | Single-cell transcriptomic landscape of human blood cells显示文摘High throughput single-cell RNA-seq has been successfully implemented to dissect the cellular and molecular features underlying hematopoiesis.However,an elaborate and comprehensive transcriptome reference of the whole blood system is lacking.Here,we profiled the transcriptomes of 7551 human blood cells representing 32 immunophenotypic cell types,including hematopoietic stem cells,progenitors and mature blood cells derived from 21 healthy donors.With high sequencing depth and coverage,we constructed a single-cell transcriptional atlas of blood cells(ABC) on the basis of both protein-coding genes and long noncoding RNAs(lncRNAs),and showed a high consistence between them.Notably,putative lncRNAs and transcription factors regulating hematopoietic cell differentiation were identified.While common transcription factor regulatory networks were activated in neutrophils and monocytes,lymphoid cells dramatically changed their regulatory networks during differentiation.Furthermore,we showed a subset of nucleated erythrocytes actively expressing immune signals,suggesting the existence of erythroid precursors with immune functions.Finally,a web portal offering transcriptome browsing and blood cell type prediction has been established.Thus,our work provides a transcriptional map of human blood cells at single-cell resolution,thereby offering a comprehensive reference for the exploration of physiological and pathological hematopoiesis. | Xiaowei Xie Mengyao Liu Yawen Zhang Bingrui Wang Caiying Zhu Chenchen Wang Qing Li Yingying Huo Jiaojiao Guo Changlu Xu Linping Hu Aiming Pang Shihui Ma Una Wang Wenbin Cao Shulian Chen Qiuling Li Sudong Zhang Xueying Zhao Wen Zhou Hongbo Luo Guoguang Zheng Erlie Jiang Sizhou Feng Lixiang Chen Lihong Shi Hui Cheng Sha Hao Ping Zhu Tao Cheng | 2021 | National Science Review2021，8，3： | 3 |
| 5 | Experimental investigation on vibration characteristics of the medium-low-speed maglev vehicle-turnout coupled system显示文摘The steel turnout is one of the key components in the medium–low-speed maglev line system.However,the vehicle under active control is prone to vehicle–turnout coupled vibration,and thus,it is necessary to identify the vibration characteristics of this coupled system through field tests.To this end,dynamic performance tests were conducted on a vehicle–turnout coupled system in a medium–low-speed maglev test line.Firstly,the dynamic response data of the coupled system under various operating conditions were obtained.Then,the natural vibration characteristics of the turnout were analysed using the free attenuation method and the finite element method,indicating a good agreement between the simulation results and the measured results;the acceleration response characteristics of the coupled system were analysed in detail,and the ride quality of the vehicle was assessed by Sperling index.Finally,the frequency distribution characteristics of the coupled system were discussed.All these test results could provide references for model validation and optimized design of medium–low-speed maglev transport systems. | Miao Li Dinggang Gao Tie Li Shihui Luo Weihua Ma Xiaohao Chen | 2022 | Railway Engineering Science2022，30，2： | 3 |
| 6 | NK cells play a significant role in immunosurveillance at the early stage of MLL-AF9 acute myeloid leukemia via CD226/CD155 interactions显示文摘Acute myeloid leukemia(AML) is an aggressive hematological malignancy, and the mechanism underlying immune system involvement in leukemia development is unclear. In the present study, we utilized a myeloid/lymphoid or mixed-lineage leukemia; translocated to, 3(MLLT3/MLL-AF9)-induced AML mouse model with or without exposure to irradiation. We found that the leukemia cells could survive and expand in hosts with intact immune systems, whereas leukemia progression was accelerated in mice with impaired immune systems. Moreover, the leukemia cells escaped from host immunosurveillance via editing their immunogenicity, including the up-regulation of an inhibitory antigen(i.e., CD47) and the down-regulation of active antigens(i.e., CD86, CD54, retinoic acid early transcript(RAE), histocompatibility 2, D region locus b(H2-Db) and H2-Dd). Natural killer(NK) cells were activated in the early phase of AML progression, whereas T cells were stimulated in the late phase. Furthermore, NK cell depletion showed that NK cells were necessary for the elimination of leukemia cells in our AML mouse model. Notably, CD155/CD226 primarily mediated the interaction between NK cells and leukemia cells and contributed to the antitumor effects of NK cells during the early phase of AML. Clinical data from patients with diverse hematological malignancies showed that CD155 expression was decreased in hematological malignancies. Taken together, our results demonstrate that NK cells play a pivotal role in immunosurveillance against leukemia cells during the early stage of AML primarily through the CD226/CD155 interaction; however, NK cells are not sufficient to eliminate leukemia cells. | WANG YaJie CHEN Chen DONG Fang MA ShiHui XU Jing GONG YueMin CHENG Hui ZHOU Yuan CHENG Tao HAO Sha | 2015 | Science China(Life Sciences)2015，58，12： | 3 |
| 7 | Stabilizing Mechanism and Running Behavior of Couplers on Heavy Haul Trains显示文摘Published studies in regard to coupler systems have been mainly focused on the manufacturing process or coupler strength issues. With the ever increasing of tonnage and length of heavy haul trains, lateral in-train forces generated by longitudinal in-train forces and coupler rotations have become a more and more significant safety issue for heavy haul train operations. Derailments caused by excessive lateral in-train forces are frequently reported. This article studies two typical coupler systems used on heavy haul locomotives. Their structures and stabilizing mechanism are analyzed before the corresponding models are developed. Coupler systems models are featured by two distinct stabilizing mechanism models and draft gear models with hysteresis considered. A model set which consists of four locomotives and three coupler systems is developed to study the rotational behavior of different coupler systems and their implications for locomotive dynamics. Simulated results indicate that when the locomotives are equipped with the type B coupler system, locomotives can meet the dynamics standard on tangent tracks; while the dynamics performance on curved tracks is very poor. The maximum longitudinal in-train force for locomotives equipped with the type B coupler system is 2000 kN. Simulations revealed a distinct trend for the type A coupler system. Locomotive dynamics are poorer for the type A case when locomotives are running on tangent tracks, while the dynamics are better for the type A case when locomotives are running on curved tracks. Theoretical studies and simulations carried out in this article suggest that a combination of the two types of stabilizing mechanism can result in a good design which can significantly decrease the relevant derailments. | XU Ziqiang WU Qing LUO Shihui MA Weihua DONG Xiaoqing | 2014 | Chinese Journal of Mechanical Engineering2014，27，6： | 2 |
| 8 | Coupler dynamic performance analysis of heavy haul locomotives显示文摘 | MA Weihua LUO Shihui SONG Rongrong | | 0，，3： | 1 |
| 9 | Analysis ofwheel set longitudinal vibration and the correlateddynamic performance显示文摘 | MA Weihua LUO Shihui SONG Rongrong | 2011 | Applied Mechanics andMaterials2011，5254，： | 1 |
| 10 | Knockdown of Burton’s tyrosine kinase confers potent protection against sepsis-induced acute lung injury显示文摘 | Panyu Zhou Bing Ma Shuogui Xu Shijie Zhang Hongtai Tang Shihui Zhu Shichu Xiao Daofeng Ben Zhaofan Xia | 2014 | Cell Biochemistry and Biophysics2014，，2： | 1 |
| 11 | Functional dissociation of the left and right fusiform gyrus in self‐face recognition显示文摘 | Yina Ma Shihui Han | 2011 | Hum Brain Mapp2011，，10： | 1 |
| 12 | Influence of track irregularity on wheelset longitudinal vibration and the correlation performance 显示文摘 | MA Weihua LUO Shihui | 2006 | Journal of Southwest Jiaotong University2006，14，3： | 1 |
| 13 | Self-face advantage is modulated by social threat – Boss effect on self-face recognition显示文摘 | Yina Ma Shihui Han | 2009 | Journal of Experimental Social Psychology2009，，4： | 1 |
| 14 | Coupler Dy- namic Performance Analysis of Heavy Haul Locomotives 显示文摘 | MA Weihua LUO Shihui SONG Rongrong | 2012 | Vehicle System Dynamics2012，50，9： | 1 |
| 15 | Phylogenetic relationship of Picea mongolica with other Picea species in the same area based on chloroplast gene variations显示文摘Picea mongolica is a conifer with a limited distribution in China and its taxonomic status is controversial.In order to explore genetic diff erences between P.mongolica and other nearby Picea species and to investigate its taxonomic status,phylogenetic relationships were analyzed between P.mongolica and Picea koraiensis,Picea meyeri and Picea wilsonii by three chloroplast gene sequences matK,chlB and atpA.The length of joint chloroplast sequence is 2379 bp.The fi fteen haplotypes were identifi ed by haplotype network analysis,among which two were major haplotypes and nine were unique.In addition,the genetic diversity of the sample collection species was inferred.Based on the haplotype network and Neighbor Joining phylogenetic tree analysis,P.mongolica was located on the basal clade of the phylogenetic tree which had more primitive taxa,and the genetic diversity of P.mongolica was highest.The signifi cant diff erences between P.mongolica and these other Picea species were identifi ed by this research. | Hao Di Jingjing Ma Kuaikuai He Fangxu Han Yue Li Shihui Niu | 2021 | Journal of Forestry Research2021，32，1： | 1 |
| 16 | Interleukin-12 supports in vitro self-renewal of long-term hematopoietic stem cells显示文摘Hematopoietic stem cells(HSCs)self-renew or differentiate through division.Cytokines are essential for inducing HSC division,but the optimal cytokine combination to control self-renewal of HSC in vitro remains unclear.In this study,we compared the effects of interleukin-12(IL-12)and thrombopoietin(TPO)in combination with stem cell factor(SCF)on in vitro self-renewal of HSCs.Single-cell assays were used to overcome the heterogeneity issue of HSCs,and serum-free conditions were newly established to permit reproduction of data.In single-cell cultures,CD150^(+)CD48^(-)CD41^(-)CD34^(-)c-Kit^(+)Sca-1^(+)lineage^(-)SCs divided significantly more slowly in the presence of SCF+IL-12 compared with cells in the presence of SCF+TPO.Serial transplantation of cells from bulk and clonal cultures revealed that TPO was more effective than IL-12 at supporting in vitro self-renewal of short-term(<6 months)HSCs,resulting in a monophasic reconstitution wave formation,whereas IL-12 was more effective than TPO at supporting the in vitro selfrenewal of long-term(>6 months)HSCs,resulting in a biphasic reconstitution wave formation.The control of division rate in HSCs appeared to be crucial for preventing the loss of self-renewal potential from their in vitro culture. | Shanshan Zhang Maiko Morita Zhao Wang Jun Ooehara Sen Zhang Miner Xie Haitao Bai Wenying Yu Xiaofang Wang Fang Dong Jinhong Wang Shihui Ma Satoshi Yamazaki Hideo Ema | 2019 | Blood Science2019，1，1： | 0 |
| 17 | Design,synthesis,and applications of stereospecific 1,3-diene carbonyls显示文摘The strategy toward the synthesis of various 1,3-dienals or 1,3-dienones is disclosed between diazo compounds and furans,which features metal-free,additive-free,broad functional group tolerance,and readily accessible starting materials.Notably,this strategy is applicable in both intramolecular and intermolecular protocols.Mechanistic studies suggested that the reactions undergo a cyclopropanation/rearrangement sequence.With an E/E-1,3-dienal,corresponding N-tosylhydrazones were readily prepared and subjected to phenylboronic acid to form a double bond migration product and indoles to construct a five-member ring via [3 + 2] annulation reaction. | Qiang Feng Shihui Wang Xingxing Ma Changqing Rao Qiuling Song | 2022 | Science China Chemistry2022，65，5： | 0 |
| 18 | Construction and application of high-quality genome-scale metabolic model of Zymomonas mobilis to guide rational design of microbial cell factories显示文摘High-quality genome-scale metabolic models(GEMs)could play critical roles on rational design of microbial cell factories in the classical Design-Build-Test-Learn cycle of synthetic biology studies.Despite of the constant establishment and update of GEMs for model microorganisms such as Escherichia coli and Saccharomyces cerevisiae,high-quality GEMs for non-model industrial microorganisms are still scarce.Zymomonas mobilis subsp.mobilis ZM4 is a non-model ethanologenic microorganism with many excellent industrial characteristics that has been developing as microbial cell factories for biochemical production.Although five GEMs of Z.mobilis have been constructed,these models are either generating ATP incorrectly,or lacking information of plasmid genes,or not providing standard format file.In this study,a high-quality GEM iZM516 of Z.mobilis ZM4 was constructed.The information from the improved genome annotation,literature,datasets of Biolog Phenotype Microarray studies,and recently updated Gene-Protein-Reaction information was combined for the curation of iZM516.Finally,516 genes,1389 reactions,1437 metabolites,and 3 cell compartments are included in iZM516,which also had the highest MEMOTE score of 91%among all published GEMs of Z.mobilis.Cell growth was then predicted by iZM516,which had 79.4%agreement with the experimental results of the substrate utilization.In addition,the potential endogenous succinate synthesis pathway of Z.mobilis ZM4 was proposed through simulation and analysis using iZM516.Furthermore,metabolic engineering strategies to produce succinate and 1,4-butanediol(1,4-BDO)were designed and then simulated under anaerobic condition using iZM516.The results indicated that 1.68 mol/mol succinate and 1.07 mol/mol 1,4-BDO can be achieved through combinational metabolic engineering strategies,which was comparable to that of the model species E.coli.Our study thus not only established a high-quality GEM iZM516 to help understand and design microbial cell factories for economic biochemical production using Z.mobilis as the chassis,but also provided guidance on building accurate GEMs for other non-model industrial microorganisms. | Yalun Wu Qianqian Yuan Yongfu Yang Defei Liu Shihui Yang Hongwu Ma | 2023 | Synthetic and Systems Biotechnology2023，8，3： | 0 |
| 19 | Highly Stereoselective C-Glycosylation by Photocatalytic Decarboxylative Alkynylation on Anomeric Position:A Facile Access to Alkynyl C-Glycosides显示文摘The highly stereoselective synthesis of diverse medicinally valuable alkynyl C-glycosides under mild and green reaction conditions remains a great challenge.Herein,we wish to report a visible-light induced photocatalytic decarboxylative alkynylation approach.By utilizing an iridium photocatalyst in the presence of visible light,glycosyl radicals are in-situ generated via the decarboxylation of anomeric acids and efficiently coupled with a variety of ethynylbenziodoxolones(EBXs),which allows the facile synthesis of a variety of alkynyl C-glycosides in moderate to high yields and with excellent diastereoselectivity. | Kailin Lu Yingying Ma Shihui Liu Shixun Guo Yongqiang Zhang | 2022 | Chinese Journal of Chemistry2022，40，6： | 0 |
| 20 | The long road to bloom in conifers显示文摘More than 600 species of conifers(phylum Pinophyta)serve as the backbone of the Earth’s terrestrial plant community and play key roles in global carbon and water cycles.Although coniferous forests account for a large fraction of global wood production,their productivity relies largely on the use of genetically improved seeds.However,acquisition of such seeds requires recurrent selection and testing of genetically superior parent trees,eventually followed by the establishment of a seed orchard to produce the improved seeds.The breeding cycle for obtaining the next generation of genetically improved seeds can be significantly lengthened when a target species has a long juvenile period.Therefore,development of methods for diminishing the juvenile phase is a cost-effective strategy for shortening breeding cycle in conifers.The molecular regulatory programs associated with the reproductive transition and annual reproductive cycle of conifers are modulated by environmental cues and endogenous developmental signals.Mounting evidence indicates that an increase in global average temperature seriously threatens plant productivity,but how conifers respond to the ever-changing natural environment has yet to be fully characterized.With the breakthrough of assembling and annotating the giant genome of conifers,identification of key components in the regulatory cascades that control the vegetative to reproductive transition is imminent.However,comparison of the signaling pathways that control the reproductive transition in conifers and the floral transition in Arabidopsis has revealed many differences.Therefore,a more complete understanding of the underlying regulatory mechanisms that control the conifer reproductive transition is of paramount importance.Here,we review our current understanding of the molecular basis for reproductive regulation,highlight recent discoveries,and review new approaches for molecular research on conifers. | Jingjing Ma Xi Chen Fangxu Han Yitong Song Biao Zhou Yumeng Nie Yue Li Shihui Niu | 2022 | Forestry Research2022，2，1： | 0 |
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