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| 1 | Study of the mechanisms of acupuncture and moxibustion treatmentfor ulcerative colitis ratsinview of the gene expression of cytokines显示文摘AIM To observe the effect of acupuncture and moxibustion on the expression of IL 1β and IL 6 mRNA in ulcerative colitis rats. METHODS The SD rat ulcerative colitis model was created by immunological method associated with local stimulation. Colonic mucosa was prepared from human fresh surgical colonic specimens, homogenized by adding appropriate amount of normal saline and centrifuged at 3000*!r/*!min . The supernatant was collected for measurement of protein conentration and then mixed with Freund adjuvant. This antigen fluid was first injected into the plantae of the model group rats, and then into their plantae, dorsa, inguina and abdominal cavities (no Freund adjuvant for the last injection) again on the 10th, 17th, 24th and 31st day. When a certain titer of serum anti colonic antibody was reached, 2% formalin and antigen fluid (no Freund adjuvant) were administered separately by enema. The ulcerative colitis rat model was thus set up. The animals were randomly divided into four groups: model control group (MC, n =8), electro acupuncture group (EA, n =8), herbs partition moxibustion group (HPM 8), normal control group (NC, n =8). HPM: Moxa cones made of refined mugwort floss were placed on the medicinal pad (medicinal pad dispensing: Radix Aconiti praeparata, cortex Cinnamomi, etc ) for Qihai (RN 6) and Tianshu (ST 25, bilateral) and ignited. Two moxa cones were used for each acupoint once a day and 14 times in all. EA: Tianshu (bilateral) and Qihai were stimulated by the intermittent pulse with 2Hz frequency, 4mA intensity for 20 minutes once a day and 14 times in all. After treatment, rats of all four groups were killed simultaneously. The spleen was separated and the distal colon was dissected. Total tissue RNA was isolated by the guanidinium thiocyanate phenol chloroform extraction method. RT PCR technique was used to study the expression of IL 1β and IL 6 mRNA. RESULTS IL 1β and IL 6 mRNAs were not detected in the spleen and colonic mucosa of the NC rats, whereas they were significantly expressed in that of the MC rats. IL 1β and IL 6 mRNAs were markedly lower in the EA and HPM rats than that in MC rats. There was no significant difference between the levels of IL 1β and IL 6 mRNAs in the EA and HPM rats. The expressions of IL 1β and IL 6 mRNAs were nearly the same in the spleen and colon of all groups. CONCLUSION Acupuncture and moxibustion greatly inhibited the expression of IL 1β and IL 6 mRNA in the experimental ulcerative colitis rats. | Wu HG Zhou LB Pan YY Huang C Chen HP Shi Z Hua XG | 1999 | World Journal of Gastroenterology1999，5，6： | 45 |
| 2 | A case-control association study between the GRID1 gene and schizophrenia in the Chinese Northern Han population 显示文摘 | Guo SZ Huang K Shi YY | 2007 | Schizophr Res2007，93，13： | 1 |
| 3 | Radical resection improves survival for patients with pT2 gallbladder carcinoma显示文摘 | Wise PE Shi YY Washington MK | 2001 | Am Surg2001，67，： | 1 |
| 4 | Identification and analysis of tumour associated antigens in hepatocellular carcinoma显示文摘 | Shi YY Wang HC Yin YH | 2005 | Brit J Cancer2005，92，5： | 1 |
| 5 | Changes in hemocytes of Plutella xylostella after parasitism by Diadegma semiclausum显示文摘 | Huang F Shi M Yang YY Li JY Chen XX | | 0，，03： | 1 |
| 6 | Hypoxia specific SDF-1 expression by retinal pigment epithelium initiates bone marrow-derived cells to participate in choroidal neovascularization in a laser-induced mouse model显示文摘 | ZHANG ZX WANG YS SHI YY HOU HY ZHANG C CAI Y | 2011 | Curt Eye Res2011，36，9： | 1 |
| 7 | Inhibition of hamster sperm acrosomal enzyme by gossypol is closely associated with decrease in fertilization capacity 显示文摘 | Yuan YY Shi QX | 2000 | Contraception2000，62，4： | 1 |
| 8 | ZNF804A and schizophrenia suscep- tibility in Asian populations 显示文摘 | Li M Shi CJ Shi YY et 81 | 2012 | American Journal of Medical Ge- netics Part B : Neuropsychiatric Genetics2012，159，： | 1 |
| 9 | Association study of single nucleotide polymorphisms in pre-miRNA and rheumatoid arthritis in a Han Chinese population 显示文摘 | Yang B Zhang JL Shi YY | 2011 | Molecular Biology Reports2011，38，8： | 1 |
| 10 | Relaxation of selective constraints on avian mitochondrial DNA following the degeneration of flight ability显示文摘 | Shen YY Shi P Sun YB | | 0，，10： | 1 |
| 11 | Adipose - derived aduh stromal ceils heal critical - size mouse calvarial defects 显示文摘 | Cowan CM Shi YY Aalami OO | 2004 | Nat Biotechnol2004，22，5： | 1 |
| 12 | Adipose -derived adult stromal eens heal critical -size mouse ealvarial defects显示文摘 | Cowan CM Shi YY Aalami OO | 2004 | Nat Biotechnol2004，22，5： | 1 |
| 13 | Identification and analysis of tumour-associated antigens in hepatocellular carcinoma显示文摘 | SHI YY WANG HC YIN YH | 2005 | Br J Cancer2005，92，5： | 1 |
| 14 | Identification and analysis of tumour-associated antigens in hepatocellular carcinoma显示文摘 | Shi YY Wang HC Yin YH | 2005 | Br J Cancer2005，92，5： | 1 |
| 15 | Effect of octreotide surface density on receptor-mediated endocytosis in vitro and anticancer efficacy of modified nanocarrierin vivo after optimization 显示文摘 | Su ZG Shi YP Xiao YY | 2013 | Ira J Pharm2013，447，12： | 1 |
| 16 | Radical resection improves survival for patients with pT2 gallbladder carcinoma 显示文摘 | Shi YY Washington MK | 2001 | AmSurg2001，67，： | 1 |
| 17 | NQO1 609C》T polymorphism interaction with tobacco smoking and alcohol drinking increases colorectal cancer risk in a Chinese population显示文摘 | Peng XE Jiang YY Shi XS | 2013 | Gene2013，521，1： | 1 |
| 18 | Corosolic acid stimulates glucose uptake via enhancing insulin receptor phosphorylation 显示文摘 | Shi L Zhang W Zhou YY | 2008 | Eur J Pharmacol2008，584，： | 1 |
| 19 | Proteasome inhibitors induce a p38 mitogen activated protein kinase (MAPK)-dependent anti apoptotie program involving MAPK phosphatase-1 and Akt in models of breast cancer显示文摘 | Shi YY Small GW Orlowski RZ | 2006 | Breast Cancer Res Treat2006，100，1： | 1 |
| 20 | Identification and analysis of tumour-associated antigens in hepatocellular carcinoma 显示文摘 | Shi YY Wang HC Yin YH | 2005 | Br J Cancer2005，92，5： | 1 |
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