|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Increasing yield potential through manipulating of an ARE1 ortholog related to nitrogen use efficiency in wheat by CRISPR/Cas9显示文摘Wheat(Triticum aestivum L.)is a staple food crop consumed by more than 30%of world population.Nitrogen(N)fertilizer has been applied broadly in agriculture practice to improve wheat yield to meet the growing demands for food production.However,undue N fertilizer application and the low N use efficiency(NUE)of modern wheat varieties are aggravating environmental pollution and ecological deterioration.Under nitrogen-limiting conditions,the rice(Oryza sativa)abnormal cytokinin response1 repressor1(are1)mutant exhibits increased NUE,delayed senescence and consequently,increased grain yield.However,the function of ARE1 ortholog in wheat remains unknown.Here,we isolated and characterized three TaARE1 homoeologs from the elite Chinese winter wheat cultivar ZhengMai 7698.We then used CRISPR/Cas9-mediated targeted mutagenesis to generate a series of transgene-free mutant lines either with partial or triple-null taare1 alleles.All transgene-free mutant lines showed enhanced tolerance to N starvation,and showed delayed senescence and increased grain yield in field conditions.In particular,the AABBdd and aabbDD mutant lines exhibited delayed senescence and significantly increased grain yield without growth defects compared to the wild-type control.Together,our results underscore the potential to manipulate ARE1 orthologs through gene editing for breeding of high-yield wheat as well as other cereal crops with improved NUE. | Jiahui Zhang Huating Zhang Shaoya Li Jingying Li Lei Yan Lanqin Xia | 2021 | Journal of Integrative Plant Biology2021，63，9： | 13 |
| 2 | A Soft Bionic Gripper with Variable Effective Length显示文摘 | Yufei Hao Zheyuan Gong Zhexin Xie Shaoya Guan Xingbang Yang Tianmiao Wang Li Wen | 2018 | Journal of Bionic Engineering2018，15，2： | 12 |
| 3 | Efficient allelic replacement in rice by gene editing: A case study of the NRT1.1B gene显示文摘Precise replacement of an existing allele in commercial cultivars with an elite allele is a major goal in crop breeding. A single nucleotide polymorphism in the NRT1.1 B gene between japonica and indica rice is responsible for the improved nitrogen use efficiency in indica rice. Herein, we precisely replaced the japonica NRT1.1 B allele with the indica allele, in just one generation, using CRISPR/Cas9 gene-editing technology.No additional selective pressure was needed to enrich the precise replacement events. This work demonstrates the feasibility of replacing any genes with elite alleles within one generation, greatly expanding our ability to improve agriculturally important traits. | Jingying Li Xin Zhang Yongwei Sun Jiahui Zhang Wenming Du Xiuping Guo Shaoya Li Yunde Zhao Lanqin Xia | 2018 | Journal of Integrative Plant Biology2018，60，7： | 11 |
| 4 | Base editing in plants: Current status and challenges显示文摘Genome editing technologies have revolutionized the field of plant science by enabling targeted modification of plant genomes and are emerging as powerful tools for both plant gene functional analyses and crop improvement. Although homology-directed repair(HDR)is a feasible approach to achieve precise gene replacement and base substitution in some plant species, the dominance of the non-homologous end joining pathway and low efficiency of HDR in plant cells have limited its application. Base editing has emerged as an alternative tool to HDR-mediated replacement, facilitating precise editing of plant genome by converting one single base to another in a programmable manner without a doublestranded break and a donor repair template. In this review, we summarize the latest developments in base-editing technologies as well as their underlying mechanisms. We review current applications of these technologies in plant species. Finally, we address the challenges and future perspectives of this emerging technology in plants. | Sutar Suhas Bharat Shaoya Li Jingying Li Lei Yan Lanqin Xia | 2020 | The Crop Journal2020，8，3： | 8 |
| 5 | Molecular characteristics of the omp A gene of serotype B Chlamydia trachomatis in Qinghai Tibetan primary school students显示文摘To study the molecular characteristics of Chlamydia trachomatis, the major outer membrane protein gene(omp A) of C. trachomatis from primary school students with trachoma residing in the Qinghai Tibetan area was sequenced and compared with the same serotype in Gen Bank. In Jianshetang Primary School and Galeng Central Primary School in the Galeng Tibetan Township of Qinghai Haidong Sala Autonomous County, scraped samples were collected from the upper tarsal conjunctiva and lower conjunctival sac of both eyes of 45 students with trachoma, stored at 4°C, and transported to Beijing Tongren Hospital by air within 24 h. The samples were screened for C. trachomatis by real-time PCR. The omp A gene from the C. trachomatis-positive samples was amplified by nested PCR. The serotype was confirmed by National Center for Biotechnology Information(NCBI) BLAST search and homology analysis. The entire omp A gene sequence was compared with the corresponding gene sequences of serotype B strains available in Gen Bank. Of the 45 students aged 6–13 years with trachoma, 26 C. trachomatis-positive students were identified by the initial real-time PCR screening(average age,(9.09±1.63) years; sex ratio, 1.0), accounting for 57.78%(26/45). The cycle threshold values for real-time PCR were 16.79–37.77. Half(13/26) of C. trachomatis-positive students had a bacterial copy number of >105. The compliance rate of the omp A gene sequences with the C. trachomatis serotype B strains in Gen Bank was up to 99%. Two novel genetic mutations were found when the omp A gene was compared with those of the 11 serotype B strains in Gen Bank. The two non-synonymous mutations were located at(i) position 271 in the second constant domain, an adenine(A) to guanine(G) substitution(ACT?GCT), changing the amino acid at position 91 from threonine to alanine(Thr?Ala) in all 26 strains; and(ii) position 887 in the fourth variable domain, a cytosine(C) to thymine(T) substitution(GCA?GTA), changing the amino acid at residue 296 from alanine to valine(Ala?Val) in four of the 26 strains. Six mutations were identified relative to ATCC VR-573. The strains could be divided into two gene clusters according to the mutation at nucleotide position 887: CQZ-1(China Qinghai Tibetan-1) and CQZ-2(China Qinghai Tibetan-2). We thus detected two novel serotype B mutant strains of C. trachomatis among study subjects with trachoma. | Xue Li Shaoya Zhang Qingfeng Liang Mei Wang Ailian Hu Xiuyuan Li Benshan Yang Mingxin Zhang Ningli Wang Xinxin Lu | 2016 | Science China(Life Sciences)2016，59，6： | 6 |
| 6 | Expanding the Scope of CRISPR/Cpfl-Mediatec Senome Editing in Rice显示文摘 | Shaoya Li Xin Zhang Wensheng Wang Xiuping Guo Zhichao wu Wenming Du Yunde Zhao Lanqin Xia | 2018 | Molecular Plant2018，11，7： | 6 |
| 7 | Pyramiding favorable alleles in an elite wheatvariety in one generation by CRISPR-Cas9-mediated multiplex gene editing显示文摘Dear Editor,Common wheat(Triticum aestivum,2n=6x=42,AABBDD)is amajor staple crop consumed by more than 30%of the world’spopulation.It is the main source of cereal-based processed prod-ucts,such as bread,cookies,pasta,and noodles.Althoughwheat production increased by 10-fold following the Green revo-lution and maker-assisted breeding over the past decades,it isstill facing unprecedented challenges in the context of globalclimate changes,growing world population,decreased farm-lands,as well as water shortages in arid and semi-arid lands.Meanwhile,the functional redundancy of genes in hexaploidywheat makes a forward genetics approach to select a desiredphenotype,especially for pyramiding of several agronomicallyimportant traits in a modern variety,very time-consuming and in some cases,impossible because of gene linkage or genedrag. | Jinman Luo Shaoya Li Jiajing Xu Lei Yan Youzhi Ma Lanqin Xia | 2021 | Molecular Plant2021，14，6： | 6 |
| 8 | Precise gene replacement in plants through CRISPR/Cas genome editing technology:current status and future perspectives显示文摘CRISPR/Cas,as a simple,versatile,robust and cost-effective system for genome manipulation,has dominated the genome editing field over the past few years.The application of CRISPR/Cas in crop improvement is particularly important in the context of global climate change,as well as diverse agricultural,environmental and ecological challenges.Various CRISPR/Cas toolboxes have been developed and allow for targeted mutagenesis at specific genome loci,transcriptome regulation and epigenome editing,base editing,and precise targeted gene/allele replacement or tagging in plants.In particular,precise replacement of an existing allele with an elite allele in a commercial variety through homology-directed repair(HDR)is a holy grail in genome editing for crop improvement as it has been very difficult,laborious and time-consuming to introgress the elite alleles into commercial varieties without any linkage drag from parental lines within a few generations in crop breeding practice.However,it still remains very challenging in crop plants.This review intends to provide an informative summary of the latest development and breakthroughs in gene replacement using CRISPR/Cas technology,with a focus on achievements,potential mechanisms and future perspectives in plant biological science as well as crop improvement. | Shaoya Li Lanqin Xia | 2020 | aBIOTECH2020，1，1： | 5 |
| 9 | Multiplex precision gene editing by a surrogate prime editor in rice显示文摘Dear Editor,Development of a multiplex precision gene editing system is highly desirable for pyramiding beneficial alleles in crop improve-ment.Prime editing is a newly developed genome-editing tool that can precisely enable the installation of all 12 nucleotide sub-stitutions,short insertions,and deletions without exogenous DNA donor repair template and double-strand breaks(Anzalone et al.,2019).Among the prime editing systems,prime editor 3(PE3),which consists of a prime editing guide RNA(pegRNA)and an additional nicking single guide RNA(sgRNA)to nick the non-edited strand,enhances editing efficiency. | Huiyuan Li Ziwei Zhu Shaoya Li Jingying Li Lei Yan Chen Zhang Youzhi Ma Lanqin Xia | 2022 | Molecular Plant2022，15，7： | 4 |
| 10 | Characteristics of pathogenic microorganisms found in 99 cases of conjunctivitis from the Qinghai Tibetan area显示文摘Dear Editor,Morbidity associated with conjunctivitis is higher in developing countries,particularly among children,because of the poorer standards of living(Azari and Barney,2013;Yetman and Coody,1997).Presently,little is known regarding the microorganisms that cause conjunctivitis in the remote area of Chinese Tibet.In particular,it is difficult to monitor and control the occurrence of conjunctivitis in children from this area.Conjunctivitis has two forms:acute and chronic. | Benshan Yang Xiuyuan Li Qingfeng Liang Shaoya Zhang Shijing Deng | 2016 | Science China(Life Sciences)2016，59，6： | 4 |
| 11 | Present and future prospects for wheat improvement through genome editing and advanced technologies显示文摘Wheat(Triticum aestivum,2n=6x=42,AABBDD)is one of the most important staple food crops in the world.Despite the fact that wheat production has significantly increased over the past decades,future wheat production will face unprecedented challenges from global climate change,increasing world population,and water shortages in arid and semi-arid lands.Furthermore,excessive applications of diverse fertilizers and pesticides are exacerbating environmental pollution and ecological deterioration.To ensure global food and ecosystem security,it is essential to enhance the resilience of wheat production while minimizing environmental pollution through the use of cutting-edge technologies.However,the hexaploid genome and gene redundancy complicate advances in genetic research and precision gene modifications for wheat improvement,thus impeding the breeding of elite wheat cultivars.In this review,we first introduce state-of-the-art genome-editing technologies in crop plants,especially wheat,for both functional genomics and genetic improvement.We then outline applications of other technologies,such as GWAS,high-throughput genotyping and phenotyping,speed breeding,and synthetic biology,in wheat.Finally,we discuss existing challenges in wheat genome editing and future prospects for precision gene modifications using advanced genome-editing technologies.We conclude that the combination of genome editing and other molecular breeding strategies will greatly facilitate genetic improvement ofwheat for sustainable global production. | Shaoya Li Chen Zhang Jingying Li Lei Yan Ning Wang Lanqin Xia | 2021 | Plant Communications2021，2，4： | 4 |
| 12 | Plant genome editing using xCas9 with expanded PAM compatibility显示文摘CRISPR/Cas enables robust genome editing and has revolution-ized both functional genomics and crop breeding.The specificity of Cas-directed DNA cleavage is strictly determined by a chimeric single guide RNA(SgRNA)and a short protospacer adjacent motif(PAM)in the genome(Cong et al,2013;Zetsche et al,2015).The widely used Cas9 from Streptococcus pyogenes(SpCas9)generally recognizes the canonical NGG PAM(where N indicates any nucleicacid base)(Miao et al.,2013;Ma et al,2015),making many regionsuntargetable by Cas9.SpCas9 VQR and VRER variants,which recog-nize the non-canonical PAM sequences of NGA and NGCG,respectively,have been used to expand targetable sequences in plants(Hu et al.,2016).In addition,the applications of other Cas endonucleases such as SaCas9(Staphylococcus aureus Cas9)(Ran et al..2015). | Jingying Li Jinman Luo Meilian Xu Shaoya Li Jiahui Zhang Huiyuan Li Lei Yan Yunde Zhao Lanqin Xia | 2019 | Journal of Genetics and Genomics2019，46，5： | 2 |
| 13 | Plant base editing and prime editing:The current status and future perspectives显示文摘Precise replacement of an allele with an elite allele controlling an important agronomic trait in a predefined manner by gene editing technologies is highly desirable in crop improvement.Base editing and prime editing are two newly developed precision gene editing systems which can introduce the substitution of a single base and install the desired short indels to the target loci in the absence of double-strand breaks and donor repair templates,respectively.Since their discoveries,various strategies have been attempted to optimize both base editor(BE)and prime editor(PE)in order to improve the precise editing efficacy,specificity,and expand the targeting scopes.Here,we summarize the latest development of various BEs and PEs,as well as their applications in plants.Based on these progresses,we recommend the appropriate BEs and PEs for both basic plant research and crop improvement.Moreover,we propose the perspectives for further optimization of these two editors.We envision that both BEs and PEs will become the routine and customized precise gene editing tools for both plant biological research and crop improvement in the near future. | Jingying Li Chen Zhang Yubing He Shaoya Li Lei Yan Yucai Li Ziwei Zhu Lanqin Xia | 2023 | Journal of Integrative Plant Biology2023，65，2： | 1 |
| 14 | Artificial evolution of OsEPSPS through an improved dual cytosine and adenine base editor generated a novel allele conferring rice glyphosate tolerance显示文摘Exploiting novel endogenous glyphosate-tolerant alleles is highly desirable and has promising potential for weed control in rice breeding. Here,through fusions of different effective cytosine and adenine deaminases with nCas9-NG, we engineered an effective surrogate two-component composite base editing system, STCBE-2, with improved C-to-T and A-to-G base editing efficiency and expanded the editing window. Furthermore,we targeted a rice endogenous OsEPSPS gene for artificial evolution through STCBE-2-mediated near-saturated mutagenesis. After hygromycin and glyphosate selection, we identified a novel OsEPSPS allele with an Asp-213-Asn(D213N)mutation(OsEPSPS-D213N) in the predicted glyphosate-binding domain, which conferred rice plants reliable glyphosate tolerance and had not been reported or applied in rice breeding. Collectively, we developed a novel dual base editor which will be valuable for artificial evolution of important genes in crops. And the novel glyphosate-tolerant rice germplasm generated in this study will benefit weeds management in rice paddy fields. | Chen Zhang Xue Zhong Shaoya Li Lei Yan Jingying Li Yubing He Yong Lin Yangjun Zhang Lanqin Xia | 2023 | Journal of Integrative Plant Biology2023，65，9： | 1 |
| 15 | Engineering a plant A-to-K base editor with improved performance by fusion with a transactivation module显示文摘Dear Editor,Base editors(BEs)are promising tools that enable single-nucleotide substitutions in specific target genes,generating loss-offunction or gain-of-function mutations,which can greatly accelerate plant functional genomics and crop improvement(Ren et al.,2018;Zeng et al.,2020;Xu et al.,2021;Yan et al.,2021;Li et al.,2023).To date,three major classes of BEs have been engineered:cytosine BEs(CBEs)for C-to-T transitions(Komor et al.,2016),adenine BEs(ABEs)for A-to-G transitions(Gaudelli et al.,2017). | Yucai Li Shaoya Li Chenfei Li Chen Zhang Lei Yan Jingying Li Yubing He Yan Guo Yong Lin Yangjun Zhang Lanqin Xia | 2023 | Plant Communications2023，4，6： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员