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您的检索式：作者名="Jinman Luo"
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| 1 | Pyramiding favorable alleles in an elite wheatvariety in one generation by CRISPR-Cas9-mediated multiplex gene editing显示文摘Dear Editor,Common wheat(Triticum aestivum,2n=6x=42,AABBDD)is amajor staple crop consumed by more than 30%of the world’spopulation.It is the main source of cereal-based processed prod-ucts,such as bread,cookies,pasta,and noodles.Althoughwheat production increased by 10-fold following the Green revo-lution and maker-assisted breeding over the past decades,it isstill facing unprecedented challenges in the context of globalclimate changes,growing world population,decreased farm-lands,as well as water shortages in arid and semi-arid lands.Meanwhile,the functional redundancy of genes in hexaploidywheat makes a forward genetics approach to select a desiredphenotype,especially for pyramiding of several agronomicallyimportant traits in a modern variety,very time-consuming and in some cases,impossible because of gene linkage or genedrag. | Jinman Luo Shaoya Li Jiajing Xu Lei Yan Youzhi Ma Lanqin Xia | 2021 | Molecular Plant2021，14，6： | 6 |
| 2 | Plant genome editing using xCas9 with expanded PAM compatibility显示文摘CRISPR/Cas enables robust genome editing and has revolution-ized both functional genomics and crop breeding.The specificity of Cas-directed DNA cleavage is strictly determined by a chimeric single guide RNA(SgRNA)and a short protospacer adjacent motif(PAM)in the genome(Cong et al,2013;Zetsche et al,2015).The widely used Cas9 from Streptococcus pyogenes(SpCas9)generally recognizes the canonical NGG PAM(where N indicates any nucleicacid base)(Miao et al.,2013;Ma et al,2015),making many regionsuntargetable by Cas9.SpCas9 VQR and VRER variants,which recog-nize the non-canonical PAM sequences of NGA and NGCG,respectively,have been used to expand targetable sequences in plants(Hu et al.,2016).In addition,the applications of other Cas endonucleases such as SaCas9(Staphylococcus aureus Cas9)(Ran et al..2015). | Jingying Li Jinman Luo Meilian Xu Shaoya Li Jiahui Zhang Huiyuan Li Lei Yan Yunde Zhao Lanqin Xia | 2019 | Journal of Genetics and Genomics2019，46，5： | 2 |
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