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| 1 | Overexpression of micro RNA_(40)8 enhances photosynthesis, growth, and seed yield in diverse plants显示文摘The ability of a plant to produce grain, fruit, or forage depends ultimately on photosynthesis. There have been few attempts, however, to study micro RNAs, which are a class of endogenous small RNAs post-transcriptionally programming gene expression, in relation to photosynthetic traits. We focused on miR_(40)8, one of the most conserved plant miRNAs, and overexpressed it in parallel in Arabidopsis, tobacco, and rice. The transgenic plants all exhibited increased copper content in the chloroplast, elevated abundance of plastocyanin,and an induction of photosynthetic genes. By means of gas exchange and optical spectroscopy analyses, we showed that higher expression of miR_(40)8 leads to enhanced photosynthesis through improving efficiency of irradiation utilization and the capacity for carbon dioxide fixation. Consequently, miR_(40)8 hyper-accumulating plants exhibited higher rate of vegetative growth. An enlargement of seed size was also observed in all three species overproducing miR_(40)8. Moreover, we conducted a 2-year-two-location field trial and observed miR_(40)8 overexpression in rice significantly increased yield, which was primarily attributed to an elevation in grain weight. Taken together, these results demonstrate that miR_(40)8 is a positive regulator of photosynthesis and that its genetic engineering is a promising route for enhancing photosynthetic performance and yield in diverse plants. | Jiawei Pan Dahui Huang Zhonglong Guo Zheng Kuang He Zhang Xinyu Xie Zengfeng Ma Shaopei Gao Manuel T.Lerdau Chengcai Chu Lei Li | 2018 | Journal of Integrative Plant Biology2018，60，4： | 8 |
| 2 | Big Grain3, encoding a purine permease, regulates grain size via modulating cytokinin transport in rice显示文摘Grain size is an important agronomic trait affecting grain yield,but the underlying molecular mechanisms remain to be elucidated.Here,we isolated a dominant mutant,big grainy(bg3-D),which exhibits a remarkable increase of grain size caused by activation of the PURINE PERMEASE gene,OsPUP4.BC3/OSPUP4 is predominantly expressed in vascular tissues and is specifically suppressed by exogenous cytokinin application.Hormone profiling revealed that the distribution of different cytokinin forms,in roots and shoots of the bg3-D mutant,is altered.Quantitative reverse transcription-PCR(qRT-PCR)analysis indicated that expression of rice cytokinin type-A RESPONSE REGULATOR(OsRR)genes is enhanced in the roots of the bgj-D mutant.These results suggest that OSPUP4 might contribute to the long-distance transport of cytokinin,by reinforcing cytokinin loading into vascular bundle cells.Furthermore,plants overexpressing OsPUP7,the closest homolog of OsPUP4,also exhibited a similar phenotype to the bg3-D mutant.Interestingly,subcellular localization demonstrated that OSPUP4 was localized on the plasma membrane,whereas OSPUP7 was localized to the endoplasmic reticulum.Based on these findings,we propose that OSPUP4 and OSPUP7 function in a linear pathway to direct cytokinin cell-to-cell transport,affecting both its long-distance movement and local allocation. | Yunhua Xiao Dapu Liu Guoxia Zhang Shaopei Gao Linchuan Liu Fan Xu Ronghui Che Yiqin Wang Hongning Tong Chengcai Chu | 2019 | Journal of Integrative Plant Biology2019，61，5： | 7 |
| 3 | Fuzzy Risk Analysis of Harbour Engineering Investment by Hierarchy System Approach显示文摘The present risk analysis model of engineering investment is built by fuzzy hierarchy approach under the assumption of maximizing the revenues of the project during its whole life cycle of operation. It can reasonably be expressed by a system evaluation analysis. As a matter of fact, the system, aimed by its system goal can be modelled by a set of factors, constitutively structured by certain links between them, to form a factorial network chart, which represents the essentials of the system behaviours, the nodes of which represent the factors concerned. The weight distribution between factors located at the same level can be determined by the eigen-value problem of a 'pair comparison' relation matrix. The weight distribution of factors at each level is successively manipulated until the fuzzy synthetic risk assessment. As an example of risk analysis of engineering investment, a harbour construction project is presented for illustration. | Lin Shaopei and Zhang Zhongming Professor, Civil and Architectural Engineering Dept., Shanghai Jiao Tong University, Shanghai Lecturer, Civil and Architectural Engineering Dept., Shanghai Jiao Tong University, Shanghai | 1992 | China Ocean Engineering1992，7，1： | 6 |
| 4 | MCPIP1 mediates inflammatory responses induced by lipopolysaccharide and lipoteichoic acid in bovine mammary epithelial cells显示文摘Monocyte chemoattractant protein-induced protein 1(MCPIP1)is a kind of zinc finger RNA binding protein,which exerts immune responses in a variety of cell types.However,the role of MCPIP1 in bovine mammary epithelial cells during mastitis has not been studied.In this study,we explored the functions of MCPIP1 in the inflammatory process induced by virulence factors of pathogens in bovine mammary alveolar cell-T(MAC-T)cell line.Our results showed that MCPIP1 was significantly highly expressed both in the mammary tissue of dairy cows with mastitis and in inflammatory MAC-T cells induced by lipopolysaccharide(LPS)or lipoteichoic acid(LTA).Furthermore,we found that overexpression of MCPIP1 in MAC-T cells abated the LPS-induced increase at the gene expression levels of inflammatory mediators tumor necrosis factor-α-α,interleukin(IL)-1β,IL-6 and IL-8,enhanced the LPS-and LTA-induced inhibition of epithelial proliferation and promoted the LPS-and LTA-induced oxidative and DNA damage.These findings indicated that MCPIP1 has an enormous potential in regulating the inflammatory response of bovine mammary epithelial cells during infection and may provide an effective therapeutic target for bovine mastitis to reduce the damage caused by inflammatory reactions. | Shaopei Qi Lixue Wang Xiao Chang Yanfen Zhang Yong Zhang Ming-Qing Gao | 2019 | Acta Biochimica et Biophysica Sinica2019，51，2： | 2 |
| 5 | Development of a high-density SSR genetic linkage map in sweet potato显示文摘Simple sequence repeat(SSR) markers have been proved to be a very powerful tool for quantitative trait locus(QTL) mapping, marker-assisted selection and comparative genomics research in many crop species. However, a high-density SSR genetic linkage map is still lacking because there are only a few SSR markers available in sweet potato. In this study, a total of 2545 simple sequence repeat(SSR) primer pairs, including 1215 genomic SSR(gSSR) primer pairs and 1330 BES-SSR(bSSR) primer pairs designed from the genome sequence and BAC-end sequence of sweet potato, respectively, were screened with sweet potato cultivars Luoxushu 8 and Zhengshu 20 and their randomly sampled two F1 individuals and 571 of them generated polymorphic bands. The selected 571 polymorphic SSR primer pairs and 35 EST-based SSR(eSSR) primer pairs developed at our laboratory were used to genotype 240 F1 individuals derived from a cross between Luoxushu 8 and Zhengshu 20. A double pseudo-test-cross strategy was applied for linkage analysis. The Luoxushu 8 map included 90 linkage groups with 5057 SSR markers and covered 13,299.9 cM with a marker density of 2.6 cM, and the Zhengshu 20 map contained 90 linkage groups with 3009 SSR markers and covered 11,122.9 cM with a marker density of 3.7 cM. Fifteen homologous groups were identified in both parent maps. These are the first SSR linkage maps consisting of the complete 90 linkage groups and 15 homologous groups, which are consistent with the autohexaploid nature of sweetpotato, and are also the linkage maps with the highest SSR marker density reported to date.These results provide a basis for QTL mapping, marker-assisted breeding and comparative genomics research of sweet potato. | Yusha Meng Chenxing Zheng Hui Li Aixian Li Hong Zhai Qingmei Wang Shaozhen He Ning Zhao Huan Zhang Shaopei Gao Qingchang Liu | 2021 | The Crop Journal2021，9，6： | 2 |
| 6 | FUZZY ECONOMIC ASSESSMENT FOR OFFSHORE OIL FIELDS显示文摘It is understood that economic assessment is a key issue in the development of offshore oil field. In view of uncertainties in exploration, engineering investment etc. the capital investment will take serious risk, especially for small marginal blocks. In order to investigate the relationships between main economic indices and uncertain factors, the upper and lower fuzzy bounds as well as the tolerance are presented in this paper based on the concept of fuzzy mathematics. Quantizing those uncertainties of concerned factors, the risk of investment can then be identified through conventional cash flow table and sensitivity analysis, consequently, the risk can be reduced through careful handling of the uncertainties. As an example, the economic analysis of a reusable platform in a marginal offshore oil field is presented, which shows the process of fuzzy assessment in exposing severe marginality of the field and the performance of discounted cash flow rate of return by the fluctuation of uncertainties. | Lin, Shaopei Sun, Qiliang Yang, Xiaofeng | 1990 | China Ocean Engineering1990，5，1： | 2 |
| 7 | Resampling Factor Estimation via Dual-Stream Convolutional Neural Network显示文摘The estimation of image resampling factors is an important problem in image forensics.Among all the resampling factor estimation methods,spectrumbased methods are one of the most widely used methods and have attracted a lot of research interest.However,because of inherent ambiguity,spectrum-based methods fail to discriminate upscale and downscale operations without any prior information.In general,the application of resampling leaves detectable traces in both spatial domain and frequency domain of a resampled image.Firstly,the resampling process will introduce correlations between neighboring pixels.In this case,a set of periodic pixels that are correlated to their neighbors can be found in a resampled image.Secondly,the resampled image has distinct and strong peaks on spectrum while the spectrum of original image has no clear peaks.Hence,in this paper,we propose a dual-stream convolutional neural network for image resampling factors estimation.One of the two streams is gray stream whose purpose is to extract resampling traces features directly from the rescaled images.The other is frequency stream that discovers the differences of spectrum between rescaled and original images.The features from two streams are then fused to construct a feature representation including the resampling traces left in spatial and frequency domain,which is later fed into softmax layer for resampling factor estimation.Experimental results show that the proposed method is effective on resampling factor estimation and outperforms some CNN-based methods. | Shangjun Luo Junwei Luo Wei Lu Yanmei Fang Jinhua Zeng Shaopei Shi Yue Zhang | 2021 | Computers, Materials & Continua2021，，1： | 1 |
| 8 | Multi-scale and multi-modal GIS-T data model显示文摘 | Shaopei Chen Jianjun Tan Christophe Claramunt Cyril Ray | 2009 | Journal of Transport Geography2009，，1： | 1 |
| 9 | On paradox of Fuzzy AI Modeling: supervised learning for rectifying fuzzy membership functions 显示文摘 | Lin Shaopei | 2005 | Artificial Intelligence Review2005，23，4： | 1 |
| 10 | Biochemical characterization of human peroxiredoxin 2, an antioxidative protein显示文摘人的 peroxiredoxin 2 (Prx2 ) ,它充满红血球,被显示了在由清除保护红血球免于氧化应力起一个关键作用象参予房间信号 transduction 一样的反应的氧种类。这里,人的 Prx2 基因成功地为 Prx2 表示被克隆进 Escherichia coli BL21 (DE3 ) 。钠 dodecyl 硫酸盐 polyacrylamide 胶化电气泳动分析建议 recombinant 蛋白质主要在一种可溶的形式被表示。recombinant 蛋白质被一步舞 Ni-nitrilotriacetic 酸 chelating 亲密关系层析净化到多达 91.5% 的纯净。Prx2 的 peroxidase 活动 H2O2 被 ferrithiocyanate 试金决定清除。Prx2 的能力被使用一个混合功能氧化系统测试保护 plasmid DNA,并且结果证明 Prx2 能阻止 DNA 经历氧化应力。紫外(紫外) 导致的房间 apoptosis 试金证明 Prx2 也能保护 NIH/3T3 房间免受紫外导致损坏的伤害,在化妆品和另外的区域建议它的可能的应用。 | Sheng Yan Shaopei Chen Zhendong Li Haiying Wang Tuxiong Huang Xiaoning Wang Jufang Wang | 2012 | Acta Biochimica et Biophysica Sinica2012，44，9： | 1 |
| 11 | A Three--dimensional Inverse Heat Conduction Problem in Estimating Surface Heat Flux by Conjugate Gradient Method显示文摘 | Huang Chenghuang Wang Shaopei | 1999 | International Journal of Heat and Mass Transfer1999，42，18： | 1 |
| 12 | A Three-Dimensio- nal Inverse Heat Conduction Problem in Estimating Sur- face Heat Flux by Conjugate Gradient Method 显示文摘 | HUAN Chenghung WANG Shaopei | 1999 | Inter- national Journal of Heat and Mass Transfer1999，42，18： | 1 |
| 13 | Multi- scale and multi-model GIS-T data model显示文摘 | Shaopei C Jianjun T Christophe C | 2011 | Journal of Transport 6eography2011，19，1： | 1 |
| 14 | An Efficient Method of Keyframe Extraction Based on a Cluster Algorithm显示文摘 | Zhang Qiang Yu Shaopei | 2013 | Journal of Human Kinetics2013，39，1： | 1 |
| 15 | Multi-scale and multi-modal transport data model显示文摘 | Chen Shaopei Tan Jianjun Claraumnt C | 2011 | Journal of Trans- port Geography2011，19，1： | 1 |
| 16 | A spatio-temporal modelling approach for the study of the connectivity and accessibility of the Guangzhou metropolitan network显示文摘 | Shaopei Chen Christophe Claramunt Cyril Ray | 2014 | Journal of Transport Geography2014，，36： | 1 |
| 17 | Rapid Dendrite Growth in Solidification of Highly Undercooled Alloys显示文摘Nonequilibrium thermodynamics and transportation kinetics near the propagating solid-liquid interface dominates the rapid solidification process,which is far from a thermodynamically stable state.Rapid solidification process can be described more precisely using quantitative thermodynamic calculation of phase diagram with nonlinear liquidus and solidus and evaluating the nonequilibrium effect in diffusion kinetics.Based on these basic principles,we used a current nonequilibrium dendrite growth model to describe rapid solidification process of deeply undercooled alloys.Evolution of the key fundamental solidification parameters was also evaluated. | 安红恩 YANG Jine ZHANG Xiaobing YANG Shaopei | 2021 | Journal of Wuhan University of Technology(Materials Science)2021，36，2： | 1 |
| 18 | Forensic Human Image Identification Using Medical Indicators显示文摘Diseases not only bring troubles to people’s body functions and mind but also influence the appearances and behaviours of human beings.Similarly,we can analyse the diseases from people’s appearances and behaviours and use the personal medical history for human identification.In this article,medical indicators presented in abnormal changes of human appearances and behaviours caused by physiological or psychological diseases were introduced,and were applied in the field of forensic identification of human images,which we called medical forensic identification of human images(mFIHI).The proposed method analysed the people’s medical signs by studying the appearance and behaviour characteristics depicted in images or videos,and made a comparative examination between the medical indicators of the questioned human images and the corresponding signs or medical history of suspects.Through a conformity and difference analysis on medical indicators and their indicated diseases,it would provide an important information for human identification from images or videos.A case study was carried out to demonstrate and verify the feasibility of the proposed method of mFIHI,and our results showed that it would be important contents and angles for forensic expert manual examination in forensic human image identification. | Jinhua Zeng Xiulian Qiu Shaopei Shi Xinwei Bian | 2022 | Forensic Sciences Research2022，7，4： | 0 |
| 19 | The transcription factor IbNAC29 positively regulates the carotenoid accumulation in sweet potato显示文摘Carotenoid is a tetraterpene pigment beneficial for human health.Although the carotenoid biosynthesis pathway has been extensively studied in plants,relatively little is known about their regulation in sweet potato.Previously,we conducted the transcriptome database of differentially expressed genes between the sweet potato(Ipomoea batatas)cultivar‘Weiduoli’and its high-carotenoid mutant‘HVB-3’.In this study,we selected one of these candidate genes,IbNAC29,for subsequent analyses.IbNAC29 belongs to the plant-specific NAC(NAM,ATAF1/2,and CUC2)transcription factor family.Relative IbNAC29 mRNA level in the HVB-3 storage roots was∼1.71-fold higher than Weiduoli.Additional experiments showed that the contents ofα-carotene,lutein,β-carotene,zeaxanthin,and capsanthin are obviously increased in the storage roots of transgenic sweet potato plants overexpressing IbNAC29.Moreover,the levels of carotenoid biosynthesis genes in transgenic plants were also up-regulated.Nevertheless,yeast one-hybrid assays indicated that IbNAC29 could not directly bind to the promoters of these carotenoid biosynthesis genes.Furthermore,the level of IbSGR1 was down-regulated,whose homologous genes in tomato can negatively regulate carotene accumulation.Yeast three-hybrid analysis revealed that the IbNAC29-IbMYB1R1-IbAITR5 could form a regulatory module.Yeast one-hybrid,electrophoretic mobility shift assay,quantitative PCR analysis of chromatin immunoprecipitation and dual-luciferase reporter assay showed that IbAITR5 directly binds to and inhibits the promoter activity of IbSGR1,up-regulating carotenoid biosynthesis gene IbPSY.Taken together,IbNAC29 is a potential candidate gene for the genetic improvement of nutritive value in sweet potato. | Shihan Xing Ruijie Li Haoqiang Zhao Hong Zhai Shaozhen He Huan Zhang Yuanyuan Zhou Ning Zhao Shaopei Gao Qingchang Liu | 2023 | Horticulture Research2023，10，3： | 0 |
| 20 | A conserved unusual posttranscriptional processing mediated by short,direct repeated (SDR) sequences in plants显示文摘In several stress responsive gene loci of monocot cereal crops,we have previously identified an unusual posttranscriptional processing mediated by paired presence of short direct repeated (SDR) sequences at 5' and 3' splicing junctions that are distinct from conventional (U2/U12-type) splicing boundaries.By using the known SDR-containing sequences as probes,24 plant candidate genes involved in diverse functional pathways from both monocots and dicots that potentially possess SDR-mediated posttranscriptional processing were predicted in the GenBank database.The SDRs-mediated posttranscriptional processing events including cis-and trans-actions were experimentally detected in majority of the predicted candidates.Extensive sequence analysis demonstrates several types of SDR-associated splicing peculiarities including partial exon deletion,exon fragment repetition,exon fragment scrambling and trans-splicing that result in either loss of partial exon or unusual exonic sequence rearrangements within or between RNA molecules.In addition,we show that the paired presence of SDR is necessary but not sufficient in SDR-mediated splicing in transient expression and stable transformation systems.We also show prokaryote is incapable of SDR-mediated premRNA splicing. | Xiangli Niu Di Luo Shaopei Gao Guangjun Ren Lijuan Chang Yuke Zhou Xiaoli Luo Yuxiang Li Pei Hou Wei Tang Bao-Rong Lu Yongsheng Liu | 2010 | Journal of Genetics and Genomics2010，37，1： | 0 |
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