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| 1 | Erianin,a novel dibenzyl compound in Dendrobium extract,inhibits lung cancer cell growth and migration via calcium/calmodulin-dependent ferroptosis显示文摘Ferroptosis,a novel form of programmed cell death,is characterized by iron-dependent lipid peroxidation and has been shown to be involved in multiple diseases,including cancer.Stimulating ferroptosis in cancer cells may be a potential strategy for cancer therapy.Therefore,ferroptosis-inducing drugs are attracting more attention for cancer treatment.Here,we showed that erianin,a natural product isolated from Dendrobium chrysotoxum Lindl,exerted its anticancer activity by inducing cell death and inhibiting cell migration in lung cancer cells.Subsequently,we demonstrated for the first time that erianin induced ferroptotic cell death in lung cancer cells,which was accompanied by ROS accumulation,lipid peroxidation,and GSH depletion.The ferroptosis inhibitors Fer-1 and Lip-1 but not Z-VAD-FMK,CQ,or necrostatin-1 rescued erianin-induced cell death,indicating that ferroptosis contributed to erianin-induced cell death.Furthermore,we demonstrated that Ca^(2+)/CaM signaling was a critical mediator of erianin-induced ferroptosis and that blockade of this signaling significantly rescued cell death induced by erianin treatment by suppressing ferroptosis.Taken together,our data suggest that the natural product erianin exerts its anticancer effects by inducing Ca^(2+)/CaMdependent ferroptosis and inhibiting cell migration,and erianin will hopefully serve as a prospective compound for lung cancer treatment. | Peng Chen Qibiao Wu Jiao Feng Lili Yan Yitian Sun Shuiping Liu Yu Xiang Mingming Zhang Ting Pan Xiaying Chen Ting Duan Lijuan Zhai Bingtao Zhai Wengang Wang Ruonan Zhang Bi Chen Xuemeng Han Yicong Li Liuxi Chen Ying Liu Xingxing Huang Ting Jin Wenzheng Zhang Hong Luo Xiaohui Chen Yongqiang Li Qiujie Li Guohua Li Qin Zhang Lvjia Zhuo Zuyi Yang Huifen Tang Tian Xie Xiaoping Ouyang Xinbing Sui | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 42 |
| 2 | Blood Lipid Profile and Prevalence of Dyslipidemia in Chinese Adults显示文摘Objective To investigate the plasma lipid levels in a national representative sample of subjects and to determine the prevalence of dyslipidemia in the Chinese population.Methods Plasma lipid profile was analyzed using the data obtained during the Chinese national nutrition and health survey(CNHS)in 2002 which involved 14 252 participants at the age of 18 years or older.Results The mean levels of total cholesterol(TC),triglyceride(TG)and high density lipoprotein cholesterol(HDL-C)in the participants were 3.81 mmol/L,1.10 mmol/L,and 1.30 mmol/L,respectively.In the groups of participants at the age of 18-44 years,45-59 years,and over 60 years the mean TC level was 3.70 mmol/L,4.09 mmol/L and 4.21 mmol/L,respectively,and the mean TG level was 07 mmol/L,1.21 mmol/L,1.20 mmol/L,1.29 mmol/L,1.33 mmol/L,and 1.33 mmol/L,respectively.The prevalence of dyslipidemia in Chinese adults was 18.6% and 22.2% in males and 15.9% in females.Dyslipidemia prevalence was higher in urban districts than in rural areas(21.0% vs.17.7%).The prevalence of hypercholesterolemia,hypertriglyceridemia,and low HDL cholesterol was 2.9%,11.9%,and 7.4% respectively among the participants.Conclusion Dyslipidemia has become one of the important health risk factors in the Chinese population.There is no significantly difference in the prevalence of dyslipidemia between the groups of participants at the age of 45-59 years and over 60 years.This study provides important lipid profile data for policy making and guideline development for the prevention of dyslipidemia in the Chinese population. | WEN-HUA ZHAO JIAN ZHANG YI ZHAI YUE YOU QING-QING MAN CHUN-RONG WANG HONG LI YING LI XIAO-GUANG YANG | 2007 | Biomedical and Environmental Sciences2007，20，4： | 22 |
| 3 | Isolation and characterization of dengue virus serotype 2 from the large dengue outbreak in Guangdong, China in 2014显示文摘Dengue has been well recognized as a global public health threat,but only sporadic epidemics and imported cases were reported in recent decades in China.Since July 2014,an unexpected large dengue outbreak has occurred in Guangdong province,China,resulting in more than 40000 patients including six deaths.To clarify and characterize the causative agent of this outbreak,the acute phase serum from a patient diagnosed with severe dengue was subjected to virus isolation and high-throughput sequencing(HTS).Traditional real-time RT-PCR and HTS with Ion Torrent PGM detected the presence of dengue virus serotype 2(DENV-2).A clinical DENV-2 isolate GZ05/2014 was obtained by culturing the patient serum in mosquito C6/36 cells.The complete genome of GZ05/2014 was determined and deposited in Gen Bank under the access number KP012546.Phylogenetic analysis based on the complete envelope gene showed that the newly DENV-2 isolate belonged to Cosmopolitan genotype and clustered closely with other Guangdong strains isolated in the past decade.No amino acid mutations that are obviously known to increase virulence or replication were identified throughout the genome of GZ05/2014.The high homology of Guangdong DENV-2 strains indicated the possibility of establishment of local DENV-2 circulation in Guangdong,China.These results help clarify the origin of this epidemic and predict the future status of dengue in China. | ZHAO Hui ZHAO Ling Zhai JIANG Tao LI Xiao Feng FAN Hang HONG Wen Xin ZHANG Yu ZHU Qin YE Qing TONG Yi Gang CAO Wu Chun ZHANG Fu Chun QIN Cheng Feng | 2014 | Science China(Life Sciences)2014，57，12： | 21 |
| 4 | Changes in Waist Circumference and Abdominal Obesity among Chinese Adults over a Ten-year Period显示文摘Objective The aim of this study was to describe changes in waist circumference(WC) and prevalence of abdominal obesity over a period of 10 years among Chinese adults in different socio-economic status(SES). Methods Data derived from the China Nutrition and Health Surveillance during 2002 and 2010-2012. We calculated the mean WC and the prevalence of abdominal obesity by gender, place of residence, SES indicators(education, income, and marital status), and body mass index(BMI) categoriesand used pooled t-tests to assess the differences between the two time periods. Results 26.0% of men and 25.3% of women had abdominal obesity in 2010-2012. The age-adjusted mean WC increased by 2.7 cm among men and 2.1 cm among women; the age-adjusted prevalence of abdominal obesity increased by 7.7% among men and 5.3% among women. The rising trends were observed in all subgroups except for a negative growth in high-income women. People living in rural areas with low education and income and with a BMI of 18.5 to 23.9 kg/m^2 had a greater absolute and relative increase in WC. People living in rural areas with low income had a greater relative increase in abdominal obesity. Conclusion The mean WC and prevalence of abdominal obesity among Chinese adults have increased during the past 10 years. Gender differences were noted using various SES indicators. | ZHAI Yi FANG Hong Yun YU Wen Tao YU Dong Mei ZHAO Li Yun LIANG Xiao Feng ZHAO Wen Hua | 2017 | Biomedical and Environmental Sciences2017，30，5： | 19 |
| 5 | Phase 1 clinical trial demonstrated that MUC1 positive metastatic seminal vesicle cancer can be effectively eradicated by modified Anti-MUC1 chimeric antigen receptor transduced T cells显示文摘Recent progress in chimeric antigen receptor-modified T-cell(CAR-T cell) technology in cancer therapy is extremely promising, especially in the treatment of patients with B-cell acute lymphoblastic leukemia. In contrast, due to the hostile immunosuppressive microenvironment of a solid tumor, CAR T-cell accessibility and survival continue to pose a considerable challenge, which leads to their limited therapeutic efficacy. In this study, we constructed two anti-MUC1 CAR-T cell lines. One set of CAR-T cells contained SM3 single chain variable fragment(sc Fv) sequence specifically targeting the MUC1 antigen and co-expressing interleukin(IL) 12(named SM3-CAR). The other CAR-T cell line carried the SM3 sc Fv sequence modified to improve its binding to MUC1 antigen(named p SM3-CAR) but did not co-express IL-12. When those two types of CAR-T cells were injected intratumorally into two independent metastatic lesions of the same MUC1+ seminal vesicle cancer patient as part of an interventional treatment strategy, the initial results indicated no side-effects of the MUC1 targeting CAR-T cell approach, and patient serum cytokines responses were positive. Further evaluation showed that p SM3-CAR effectively caused tumor necrosis, providing new options for improved CAR-T therapy in solid tumors. | Fengtao You Licui Jiang Bozhen Zhang Qiang Lu Qiao Zhou Xiaoyang Liao Hong Wu Kaiqi Du Youcai Zhu Huimin Meng Zhishu Gong Yunhui Zong Lei Huang Man Lu Jirong Tang Yafen Li Xiaochen Zhai Xiangling Wang Sisi Ye Dan Chen Lei Yuan Lin Qi Lin Yang | 2016 | Science China(Life Sciences)2016，59，4： | 16 |
| 6 | Distribution,fluxes and decadal changes of nutrients in the Jiulong River Estuary,Southwest Taiwan Strait显示文摘The Jiulong River Estuary (JRE) is a typical subtropical macro-tide estuary on the southwest coast of the Taiwan Strait (TWS),which has been greatly impacted by human activities over the past 30 years.To understand nutrient dynamics and fluxes under such a heavy background of anthropogenic perturbation,eight cruises were conducted from April 2008 to April 2011,covering both wet (May to September) and dry (October to April next year) seasons.Nutrient concentrations were very high for the freshwater end-member in the upper reach of the JRE (nitrate (NO 3-N):120-230 mol L-1 ;nitrite (NO 2-N):5-15 mol L-1 ;ammonium (NH 4-N):15-170 mol L-1 ;soluble reactive phosphorus (SRP):1.2-3.5 mol L-1 ;dissolved silicate (DSi):200-340 mol L-1).In dry seasons,concentrations of these nutrients were higher than in wet seasons.Nitrate was the dominant chemical species of dissolved inorganic nitrogen (DIN),with percentages of 67%-96% in wet seasons and 55%-72% in dry seasons.Distributions of NO 3-N and DSi against salinity were nearly constant during all cruises,and showed generally conservative mixing behaviors in the estuary (1 | YAN XiuLi ZHAI WeiDong HONG HuaSheng LI Yan GUO WeiDong HUANG Xiao | 2012 | Chinese Science Bulletin2012，57，18： | 16 |
| 7 | Potential of essential oils for poultry and pigs显示文摘The increasing pressure of abolishing and/or decreasing the use of antibiotics as antimicrobial growth promoters for livestock calls for alternative solutions to sustain the efficiency of current livestock production.Among the alternatives, essential oils have a great potential and are generally considered natural, less toxic, and free from residues.Essential oils have been proven in numerous in vitro studies to exert antimicrobial effects on various pathogens.The current review touched on the basics of essential oils, and the in vivo effects of essential oils on growth, intestinal microflora, anti-oxidation, immune functionality, meat qualities as well as the possible modes of action in poultry and pigs, and the future research areas were proposed. | Hengxiao Zhai Hong Liu Shikui Wang Jinlong Wu Anna-Maria Kluenter | 2018 | Animal Nutrition2018，，2： | 16 |
| 8 | Late Mesoarchean crust growth event: evidence from the ca. 2.8 Ga granodioritic gneisses of the Xiaoqinling area, southern North China Craton显示文摘Recently, more attention has been paid to Precambrian magma events and crustal growth of the North China Craton(NCC), accompanying with controversy in activity stages and dynamic mechanism. In this study, we report the Archean(2802 ± 13 Ma) granodioritic gneisses in the Taihua Complex from the Xiaoqinling area, located in the southern margin of the NCC. The zircon Lu–Hf isotope analysis of the rocks showed ^(176)Hf/^(177)Hf ratios of0.280977–0.281228, corresponding to ε_(Hf)(t) values ranging from-3.5 to +6.6, and two–stage Hf model ages varying from 2836 to 3409 Ma. It was confirmed that late Mesoarchean(2.9–2.8 Ga) juvenile crust made contribution to the source material of these ca. 2.8 Ga granodioritic gneisses in the Xiaoqinling area. The whole rock geochemical data indicate that the granodioritic gneisses are high-K calc-alkaline series, probably generated at relatively high pressure and temperature, and formed under the continental arc setting. Statistically, we conclude that the magmatic activities during 2.9–2.7 Ga may represent the most intense crustal growth events in the NCC and these Archean rocks at different locations in southern NCC underwent a similar crustal evolution history. | Xiaoliang Jia Xiyan Zhu Mingguo Zhai Yan Zhao Hong Zhang Jialin Wu Tao Liu | 2016 | Science Bulletin2016，61，12： | 16 |
| 9 | Progress in Global Gas Hydrate Development and Production as a New Energy Resource显示文摘Natural gas hydrates have been hailed as a new and promising unconventional alternative energy,especially as fossil fuels approach depletion,energy consumption soars,and fossil fuel prices rise,owing to their extensive distribution,abundance,and high fuel efficiency.Gas hydrate reservoirs are similar to a storage cupboard in the global carbon cycle,containing most of the world's methane and accounting for a third of Earth's mobile organic carbon.We investigated gas hydrate stability zone burial depths from the viewpoint of conditions associated with stable existence of gas hydrates,such as temperature,pressure,and heat flow,based on related data collected by the global drilling programs.Hydrate-related areas are estimated using various biological,geochemical and geophysical tools.Based on a series of previous investigations,we cover the history and status of gas hydrate exploration in the USA,Japan,South Korea,India,Germany,the polar areas,and China.Then,we review the current techniques for hydrate exploration in a global scale.Additionally,we briefly review existing techniques for recovering methane from gas hydrates,including thermal stimulation,depressurization,chemical injection,and CH4-CO2 exchange,as well as corresponding global field trials in Russia,Japan,United States,Canada and China.In particular,unlike diagenetic gas hydrates in coarse sandy sediments in Japan and gravel sediments in the United States and Canada,most gas hydrates in the northem South China Sea are non-diagenetic and exist in fine-grained sediments with a vein-like morphology.Therefore,especially in terms of the offshore production test in gas hydrate reservoirs in the Shenhu area in the north slope of the South China Sea,Chinese scientists have proposed two unprecedented techniques that have been verified during the field trials:solid fluidization and formation fluid extraction.Herein,we introduce the two production techniques,as well as the so-called'fbur-in-one'environmental monitoring system employed during the Shenhu production test.Methane is not currently commercially produced from gas hydrates anywhere in the world;therefore,the objective of field trials is to prove whether existing techniques could be applied as feasible and economic production methods for gas hydrates in deep-water sediments and permafrost zones.Before achieving commercial methane recovery from gas hydrates,it should be necessary to measure the geologic properties of gas hydrate reservoirs to optimize and improve existing production techniques.Herein,we propose horizontal wells,multilateral wells,and cluster wells improved by the vertical and in dividual wells applied during existing field trials.It is noteworthy that relatively pure gas hydrates occur in seafloor mounds,within near-surface sediments,and in gas migration conduits.Their extensive distribution,high saturation,and easy access mean that these types of gas hydrate may attract considerable attention from academia and industry in the future.Herein,we also review the occurrence and development of concentrated shallow hydrate accumulations and briefly introduce exploration and production techniques.In the closing section,we discuss future research needs,key issues,and major challenges related to gas hydrate exploration and production.We believe this review article provides insight on past,present,and future gas hydrate exploration and production to provide guidelines and stimulate new work into the field of gas hydrates. | LIU Liping SUN Zhilei ZHANG Lei WU Nengyou Yichao Qin JIANG Zuzhou GENG Wei CAO Hong ZHANG Xilin ZHAI Bin XU Cuiling SHEN Zhicong JIA Yonggang | 2019 | Acta Geologica Sinica(English Edition)2019，93，3： | 13 |
| 10 | Inactivated Sendai Virus Suppresses Murine Melanoma Growth by Inducing Host Immune Responses and Down-regulating β-catenin Expression显示文摘Objective This paper aims to investigate the anti-tumor mechanism of inactivated Sendai virus (Hemagglutinating virus of Japan envelope, HVJ-E) for murine melanoma (B16F10). Methods The murine dendritic cells (DCs) were treated with HVJ-E, and then the cytokines secreted from DCs and costimulation-related molecules on DCs were measured. Meanwhile, the expression of β-catenin in HVJ-E treated murine melanoma cells was detected. In addition, HVJ-E was intratumorally injected into the melanoma on C57BL/6 mice, and the immune cells, CTL response and tumor volume were analyzed. Results HVJ-E injected into B16F10 melanoma obviously inhibited the growth of the tumor and prolonged the survival time of the tumor-bearing mice. Profiles of cytokines secreted by dendritic cells (DCs) after HVJ-E stimulation showed that the number of cytokines released was significantly higher than that elicited by PBS (P<0.05). The co-stimulation-related molecules on DCs were comparable to those stimulated by LPS. Immunohistochemical examinations demonstrated the repression of β-catenin in B16F10 melanoma cells after HVJ-E treatment. Meanwhile, real-time reverse transcription PCR revealed that HVJ-E induced a remarkable infiltration of CD11c positive cells, chemokine ligand 10 (CXCL10) molecules, interleukin-2 (IL-2) molecule, CD4 + and CD8 + T cells into HVJ-E injected tumors. Furthermore, the mRNA expression level of β-catenin in the HVJ-E injected tumors was also down-regulated. In addition, B16F10-specific CTLs were induced significantly after HVJ-E was injected into the tumor-bearing mice. Conclusion This is the first report to show the effective inhibition of melanoma tumors by HVJ-E alone and the mechanism through which it induces antitumor immune responses and regulates important signal pathways for melanoma invasion. Therefore, HVJ-E shows its prospect as a novel therapeutic for melanoma therapy. | ZHANG Quan YUAN Wei Feng ZHAI Guo Qin XUE Zheng Feng ZHU Hong Fei XU Xiang Ming | 2012 | Biomedical and Environmental Sciences2012，25，5： | 9 |
| 11 | Molecular Cloning and Functional Characterization of a Salt Tolerance-Associated Gene IbNFU1 from Sweetpotato显示文摘Iron-sulfur cluster biosynthesis involving the nitrogen fixation(Nif) proteins has been proposed as a general mechanism acting in various organisms.NifU-like protein may play an important role in protecting plants against abiotic and biotic stresses.Based on the EST sequence selected from salt-stressed suppression subtractive hybridization(SSH) cDNA library constructed with a salt-tolerant mutant LM79,a NFU gene,termed IbNFU1,was cloned from sweetpotato(Ipomoea batatas(L.) Lam.) via rapid amplification of cDNA ends(RACE).The cDNA sequence of 1 117 bp contained an 846 bp open reading frame encoding a 281 amino acids polypeptide with a molecular weight of 30.5 kDa and an isoelectric point(pI) of 5.12.IbNFU1 gene contained a conserved Cys-X-X-Cys motif in C-terminal of the iron-sulfur cluster domain.The deduced amino acid sequence had 66.08 to 71.99% sequence identity to NFU genes reported in Arabidopsis thaliana,Eucalyptus grandis and Vitis vinifera.Real-time quantitative PCR analysis revealed that the expression level of IbNFU1 gene was significantly higher in the roots of the mutant LM79 compared to the wild-type Lizixiang.Transgenic tobacco(cv.Wisconsin 38) plants expressing IbNFU1 gene exhibited significantly higher salt tolerance compared to the untransformed control plants.It is proposed that IbNFU1 gene has an important function for salt tolerance of plants. | WANG Lian-jun HE Shao-zhen ZHAI Hong LIU De-gao WANG Yan-nan LIU Qing-chang | 2013 | Journal of Integrative Agriculture2013，12，1： | 9 |
| 12 | AFLP Fingerprinting and Genetic Diversity of Main Sweetpotato Varieties in China显示文摘AFLP fingerprinting of the 98 main sweetpotato varieties planted in China has been constructed. Using 17 AFLP primer combinations which were selected from 1 208 primer combinations and generated the most amounts of polymorphic bands, AFLP analysis of the 98 main sweetpotato varieties gave a total of 410 clear polymorphic bands with an average of 24.12 polymorphic bands per primer combination. Each one of the 98 sweetpotato varieties could be clearly distinguished by EcoR I-cta/Mse I-ggc primer combination which generated the most polymorphic bands. AFLP-based genetic distance ranged from 0.0546 to 0.5709 with an average of 0.3799. The dendrogram based on AFLP markers indicated that sweetpotato varieties coming from the same regions or having same parents were clustered in the same groups. Analysis of molecular variance (AMOVA) revealed greater variations within regions (94.08%) than among regions (5.92%). Thus, the genetic variations mainly existed within regions, while the variations among regions were very low in the tested sweetpotato varieties. Significant genetic variations existed between 'Northern'and 'Southern'sweetpotato varieties when Yangtze River was used as the dividing line. | LIU De-gao ZHAO Ning ZHAI Hong YU Xiao-xia JIE Qin WANG Lian-jun HE Shao-zhen LIUQing-chang | 2012 | Journal of Integrative Agriculture2012，11，9： | 9 |
| 13 | High Staphylococcus epidermidis Colonization and Impaired Permeability Barrier in Facial Seborrheic Dermatitis显示文摘 | Qian An Meng Sun Rui-Qun Qi Li Zhang Jin-Long Zhai Yu-Xiao Hong Bing Song Hong-Duo Chen Xing-Hua Gao | 2017 | Chinese Medical Journal2017，，14： | 8 |
| 14 | Cloning and Characterization of a Salt Tolerance-Associated Gene Encoding Trehalose-6-Phosphate Synthase in Sweetpotato显示文摘Trehalose plays an important role in metabolic regulation and abiotic stress tolerance in a variety of organisms. In plants, its biosynthesis is catalyzed by two key enzymes: trehalose-6-phosphate synthase(TPS) and trehalose-6-phosphate phosphatase(TPP). In the present study, a TPS gene, named IbTPS, was first isolated from sweetpotato(Ipomoea batatas(L.) Lam.) cv. Lushu 3 by rapid amplification of cDNA ends(RACE). The open reading frame(ORF) contained 2 580 nucleotides encoding 859 amino acids with a molecular weight of 97.433 kDa and an isoelectric point(pI) of 5.7. The deduced amino acid sequence showed high identities with TPS of other plants. Real-time quantitative PCR analysis revealed that the expression level of IbTPS gene was significantly higher in stems of Lushu 3 than in its leaves and roots. Subcellular localization analysis in onion epidermal cells indicated that IbTPS gene was located in the nucleus. Transgenic tobacco(cv. Wisconsin 38) plants over-expressing IbTPS gene exhibited significantly higher salt tolerance compared with the control plant. Trehalose and proline content was found to be significantly more accumulated in transgenic tobacco plants than in the wild-type and several stress tolerance related genes were up-regulated. These results suggest that IbTPS gene may enhance salt tolerance of plants by increasing the amount of treahalose and proline and regulating the expression of stress tolerance related genes. | JIANG Tao ZHAI Hong WANG Fei-bing ZHOU Hua-nan SI Zeng-zhi HE Shao-zhen LIU Qing-chang | 2014 | Journal of Integrative Agriculture2014，13，8： | 8 |
| 15 | Expression of Recombinant Human Lysozyme-tachyplesin I(hLYZ-TP I)in Pichia Pastoris and Analysis of Antibacterial Activity显示文摘Antimicrobial peptides(AMPs)are making headlines in science because they demonstrate superior microbicidal characteristics compared to synthetic and semi-synthetic antibiotics [1].Importantly,AMPs kill pathogens via a different mechanism compared to antibiotics [2].Therefore, | GAO Yu ZHAO Hong Lei FENG Xin ZHAI Rui Dong ZHU Seng DU Chong Tao SUN Chang Jiang LEI Lian Cheng | 2013 | Biomedical and Environmental Sciences2013，26，4： | 7 |
| 16 | SSR fingerprinting of 203 sweetpotato (Ipomoea batatas (L.) Lam.) varieties显示文摘Simple sequence repeat(SSR) markers have been shown to be a powerful tool for varieties identification in plants.However,SSR fingerprinting of sweetpotato varieties has been a little reported.In this study,a total of 1 294 SSR primer pairs,including 1 215 genomic-SSR and 79 expressed sequence tag(EST)-SSR primer pairs,were screened with sweetpotato varieties Zhengshu 20 and Luoxushu 8 and their 2 F1 individuals randomly sampled,and 273 and 38 of them generated polymorphic bands,respectively.Four genomic-SSR and 3 EST-SSR primer pairs,which showed good polymorphism,were selected to amplify 203 sweetpotato varieties and gave a total of 172 bands,85(49.42%) of which were polymorphic.All of the 203 sweetpotato varieties showed unique fingerprint patterns,indicating the utility of SSR markers in variety identification of this crop.Polymorphism information content(PIC) ranged from 0.5824 to 0.9322 with an average of 0.8176.SSR-based genetic distances varied from 0.0118 to 0.6353 with an average of 0.3100 among these varieties.Thus,these sweetpotato varieties exhibited high levels of genetic similarity and had distinct fingerprint profiles.The SSR fingerprints of the 203 sweetpotato varieties have been successfully constructed.The highly polymorphic SSR primer pairs developed in this study have the potential to be used as core primer pairs for variety identification,genetic diversity assessment and linkage map construction in sweetpotato and other plants. | MENG Yu-sha ZHAO Ning LI Hui ZHAI Hong HE Shao-zhen LIU Qing-chang | 2018 | Journal of Integrative Agriculture2018，17，1： | 7 |
| 17 | Photo-production of dissolved inorganic carbon from dissolved organic matter in contrasting coastal waters in the southwestern Taiwan Strait, China显示文摘Photo-production of dissolved inorganic carbon (DIC) from chromophoric dissolved organic matter (CDOM) is an important transformation process in marine carbon cycle, but little is known about this process in Chinese coastal systems. This study investigated an estuarine water sample and a coastal seawater sample from the subtropical waters in southeast of China. Water samples were exposed to natural sunlight and the absorption and fluorescence of CDOM as well as the DIC concentration were measured in the summer of 2009. The estuarine water had higher CDOM level, molecular weight and proportion of humic-like fluorescent components than the seawater that exhibited abundant tryptophan-like fluorescent component. After a 3-day irradiation, the CDOM level decreased by 45% in the estuarine water and 20% in the seawater, accompanied with a decrease in the molecular weight and aromaticity of DOM which was inferred from an increase in the absorption spectral slope parameter. The photo-degradation rates of all the five fluorescent components were also notable, in particular two humic-like components (C4 and C5) were removed by 78% and 69% in the estuarine water and by 69% and 56% in the seawater. The estuarine water had a higher photo-production rate of DIC than the seawater (4.4 vs. 2.5 μmol/(L·day)), in part due to its higher CDOM abundance. The differences in CDOM compositions between the two types of waters might be responsible for the higher susceptibility of the estuarine water to photo-degradation and hence could also affect the photo-production process of DIC. | Weidong Guo Liyang Yang Xiangxiang Yu Weidong Zhai Huasheng Hong | 2012 | Journal of Environmental Sciences2012，24，7： | 7 |
| 18 | Delivery room resuscitation and short-term outcomes of extremely preterm and extremely low birth weight infants: a multicenter survey in North China显示文摘Background:Delivery room resuscitation assists preterm infants,especially extremely preterm infants(EPI)and extremely low birth weight infants(ELBWI),in breathing support,while it potentially exerts a negative impact on the lungs and outcomes of preterm infants.This study aimed to assess delivery room resuscitation and discharge outcomes of EPI and ELBWI in China.Methods:The clinical data of EPI(gestational age[GA]<28 weeks)and ELBWI(birth weight[BW]<1000 g),admitted within 72 h of birth in 33 neonatal intensive care units from five provinces and cities in North China between 2017 and 2018,were analyzed.The primary outcomes were delivery room resuscitation and risk factors for delivery room intubation(DRI).The secondary outcomes were survival rates,incidence of bronchopulmonary dysplasia(BPD),and risk factors for BPD.Results:A cohort of 952 preterm infants were enrolled.The incidence of DRI,chest compressions,and administration of epinephrine was 55.9%(532/952),12.5%(119/952),and 7.0%(67/952),respectively.Multivariate analysis revealed that the risk factors for DRI were GA<28 weeks(odds ratio[OR],3.147;95%confidence interval[CI],2.082–4.755),BW<1000 g(OR,2.240;95%CI,1.606–3.125),and antepartum infection(OR,1.429;95%CI,1.044–1.956).The survival rate was 65.9%(627/952)and was dependent on GA.The rate of BPD was 29.3%(181/627).Multivariate analysis showed that the risk factors for BPD were male(OR,1.603;95% CI,1.061–2.424),DRI(OR,2.094;95% CI,1.328–3.303),respiratory distress syndrome exposed to≥2 doses of pulmonary surfactants(PS;OR,2.700;95%CI,1.679–4.343),and mechanical ventilation≥7 days(OR,4.358;95% CI,2.777–6.837).However,a larger BW(OR,0.998;95% CI,0.996–0.999),antenatal steroid(OR,0.577;95%CI,0.379–0.880),and PS use in the delivery room(OR,0.273;95%CI,0.160–0.467)were preventive factors for BPD(all P<0.05).Conclusion:Improving delivery room resuscitation and management of respiratory complications are imperative during early management of the health of EPI and ELBWI. | Shuai-Jun Li Qi Feng Xiu-Ying Tian Ying Zhou Yong Ji Yue-Mei Li Shu-Fen Zhai Wei Guo Fang Zhang Rong-Xiu Zheng Hai-Ying He Xia Liu Jun-Yi Wang Hua Mei Hong-Yun Wang Hua Xie Chao-Mei Zeng Li Ma Ping-Ping Zhang Jin-Yu Li Xiao-Ying Wang Li-Hua Li Hong Cui Shu-Lan Yang Lu Chen Xiao-Hong Gu Yan-Ju Hu Sheng-Shun Que Li-Xia Sun Ming Yang Wen-Li Zhao Qiu-Yan Ma Hai-Juan Wang Jiu-Ye Guo | 2021 | Chinese Medical Journal2021，，13： | 7 |
| 19 | Overexpression of SOS Genes Enhanced Salt Tolerance in Sweetpotato显示文摘The production of transgenic sweetpotato (cv.Xushu 18) plants exhibiting enhanced salt tolerance using salt overly sensitive (SOS) genes was achieved through Agrobacterium tumefaciens-mediated transformation.A.tumefaciens strain EHA105 harbors a binary vector pCAMBIA3301 with SOS genes (SOS1,SOS2 and SOS3) and bar gene.Selection culture was conducted using 0.3 mg L-1 phosphinothricin (PPT).A total of 40 plants were produced from the inoculated 170 cell aggregates via somatic embryogenesis.PCR analysis showed that 37 of the 40 regenerated plants were transgenic plants.The in vitro assay demonstrated that superoxide dismutase (SOD) and proline were significantly more accumulated and malonaldehyde (MDA) was significantly less accumulated in 21 transgenic plants than in control plants when they were exposed to 86 mmol L-1 NaCl.Salt tolerance of these 21 plants was further evaluated with Hoagland solution containing 0,51,86,and 120 mmol L-1 NaCl in the greenhouse.The results indicated that 6 of them had significantly better growth and rooting ability than the remaining 15 transgenic plants and control plants.Expression of SOS genes in the 6 salt-tolerant transgenic plants was demonstrated by RT-PCR analysis.This study provides an alternative approach for improving salt tolerance of sweetpotato. | GAO Shang YUAN Li ZHAI Hong LIU Cheng-long HE Shao-zhen LIU Qing-chang | 2012 | Journal of Integrative Agriculture2012，11，3： | 7 |
| 20 | Assessment and Comparison of in vitro Immunoregulatory Activity of Three Astaxanthin Stereoisomers显示文摘In recent years,the immune-modulatory role of all-trans astaxanthin from different pigment sources has been studied.It was reported that all-trans astaxanthin might exist as three stereoisomers,and the composition of all-trans stereoisomers in natural materials differs from that of synthetic products.However,the different biological effects of various all-trans stereoisomers still remain unclear.In the present study,we evaluated the bioactivity of three astaxanthin stereoisomers,(3S,3'S)-trans-,(3R,3'R)-transand meso-trans-astaxanthin,in regulating cell-mediated immune response using mice lymphocytes and peritoneal exudates cells(PECs) systems.After the treatment with three astaxanthin stereoisomers(20 μmol L-1),the lymphocyte proliferation capacity,neutral red phagocytosis of PECs and natural killer(NK) cell cytotoxic activity were comparatively assessed.The results showed that all three astaxanthin stereoisomers significantly promoted lymphocyte proliferation,phagocytic capacity of PECs,and cytotoxic activity of NK cells.Moreover,the(3S,3'S)-trans-astaxanthin exhibited a much higher response than others. | SUN Weihong XING Lihong LIN Hong LENG Kailiang ZHAI Yuxiu LIU Xiaofang | 2016 | Journal of Ocean University of China2016，15，2： | 7 |
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