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| 1 | Online LS-SVM for function estimation and classification显示文摘An online algorithm for training LS-SVM (Least Square Support Vector Machines) was proposed for the application of function estimation and classification. Online LS-SVM means that LS-SVM can be trained in an incremental way, and can be pruned to get sparse approximation in a decremental way. When a SV (Support Vector) is added or removed, the online algorithm avoids computing large-scale matrix inverse. Thus the computation cost is reduced. Online algorithm is especially useful to realistic function estimation problem such as system identification. The experiments with benchmark function estimation problem and classification problem show the validity of this online algorithm. | JianghuaLiu Jia-pinChen ShanJiang JunshiCheng | 2003 | Journal of University of Science and Technology Beijing2003，10，5： | 7 |
| 2 | 电子学习——未来英语学习的最佳途径(英文)显示文摘英语,作为一种全球通用的语言,已经越来越显示出其霸主的地位。近来,因特网的出现为英语学习大开方便之门。中国的顺利入世和申奥的成功再次掀起了英语学习的高潮。然而,我国教育资源的困乏给英语学习带来很大障碍。由于英语学习的需求不断加大,传统的课堂教学手段已无法满足人们学习英语的需要。随着高科技的发展,方便快捷的因特网已经成为人们生活中必不可少的一部分。借助于因特网的新兴教育产业——电子学习(E-Learning),亦称在线学习(Online-Learning)成为英语学习的最佳媒介,为英语爱好者搭起一个有效的学习平台。本文通过电子学习手段同传统学习手段的比较,阐明电子学习是未来英语学习的最佳途径。 | Yao Li Shenyang Institute of Technology Cao Ying & Wang Shanjiang Dalian University | 2002 | Chinese Journal of Applied Linguistics2002，25，1： | 3 |
| 3 | Observation of a bi-critical point between antiferromagnetic and superconducting phases in pressurized single crystal Ca0.73La0.27FeAs2显示文摘One of the most strikingly universal features of the high-temperature superconductors is that the superconducting phase emerges in the close proximity of the antiferromagnetic phase, and the interplay between these two phases poses a long-standing challenge. It is commonly believed that, as the antiferromagnetic transition temperature is continuously suppressed to zero, there appears a quantum critical point, around which the existence of antiferromagnetic fluctuation is responsible for the development of the superconductivity. In contrast to this scenario, we report the observation of a bi-critical point identified at 2.88 GPa and 26.02 K in the pressurized high-quality single crystal Ca_(0.73)La_(0.27)FeAs_2 by complementary in-situ high pressure measurements. At the critical pressure, we find that the antiferromagnetism suddenly disappears and superconductivity simultaneously emerges at almost the same temperature, and that the external magnetic field suppresses the superconducting transition temperature but hardly affects the antiferromagnetic transition temperature. | Yazhou Zhou ShanJiang Qi wu Vladimir A.Sidorov Jing Guo Wei Yi Shan Zhang Zhe Wang HonghongWang shu Cai Ke Yang Sheng Jiang Aiguo Li Ni Ni Guangming Zhang Liling Sun Zhongxian Zhao | 2017 | Science Bulletin2017，62，12： | 3 |
| 4 | Preparation of anti-mouse caspase-12 mRNA hammerhead ribozyme and identification of its activity in vitro显示文摘AIM: To prepare and identify specific anti-mouse caspase12 hammerhead ribozymes in vitro, in order to select a more effective ribozyme against mouse caspase-12 as a potential tool to rescue cells from endoplasmic reticulum stress induced apoptosis.METHODS: Two hammerhead ribozymes directed separately against 138 and 218 site of nucleotide of mouse caspase-12 mRNA were designed by computer software,and their DNA sequences were synthesized. The synthesized ribozymes were cloned into an eukaryotic expression vector-neorpBSKU6 and embedded in U6 SnRNA context for further study. Mouse caspase-12 gene segment was cloned into PGEM-T vector under the control of T7 RNA polymerase promoter (containing gene sequence from positions nt 41 to nt 894) as target. In vitro transcription both the ribozymes and target utilize T7 promoter. The target was labeled with [α-32P]UTP, while ribozymes were not labeled. After gel purification the RNAs were dissolved in RNase free water. Ribozyme and target were incubated for 90 min at 37 ℃ in reaction buffer (40 mmol/L Tris-HCL,pH 7.5, 10 mmol/L Mg2+). Molar ratio of ribozyme vs target was 30:1. Samples were analyzed on 6% PAGE (containing 8 mol/L urea).RESULTS: Both caspase-12 and ribozyme gene sequences were successfully cloned into expression vector confirmed by sequencing. Ribozymes and caspase-12 mRNA were obtained by in vitro transcription. Cleavage experiment showed that in a physiological similar condition (37 ℃,pH 7.5), Rz138 and Rz218 both cleaved targets at predicted sites, for Rz138 the cleavage efficiency was about 100%,for Rz218 the value was 36.66%.CONCLUSION: Rz138 prepared in vitro can site specific cleave mouse caspase-12 mRNA with an excellent efficiency. It shows a potential to suppress the expression of caspase-12 in vivo, thus provided a new way to protect cells from ER stress induced apoptosis. | ShanJiang QingXie WeiZhang Xia-QiuZhou You-XinJin | 2005 | World Journal of Gastroenterology2005，11，26： | 2 |
| 5 | Sesamin as a co-initiator for UV photopolymeri-zation显示文摘 | KEMIN WANG SHANJIANG QIANG YU | 2011 | Chemistry and Materials Science2011，53，3： | 1 |
| 6 | EasyPDP: An Efficient Parallel Dynamic Programming Runtime System for Computational Biology显示文摘 | Tang Shanjiang Yu Ce Sun Jizhou | 2012 | IEEE Transactions on Parallel and Distributed Systems2012，23，5： | 1 |
| 7 | Inhibitory effects of polyphenols toward HCV from the mangrove plant Excoecaria agallocha L.显示文摘 | Yongxin Li Shanjiang Yu Dong Liu Peter Proksch Wenhan Lin | 2011 | Bioorganic & Medicinal Chemistry Letters2011，，2： | 1 |
| 8 | Expression and in vitro cleavage activity of anti-caspase-7 hammerhead ribozymes显示文摘AIM:To prepare hammerhead ribozymes against mouse caspase-7 and identify their cleavage activity in vitro, in order to select a ribozyme with specific cleavage activity against mouse caspase-7 as a potential gene therapy for apoptosis-related diseases.METHODS: Anti-caspase-7 ribozymes targeting sites 333 and 394 (named Rz333 and Rz394) were designed by computer software, and their DNA sequences encoding ribozymes were synthesized. Caspase-7 DNA sequence was acquired by RT-PCR. Ribozymes and caspase-7 DNA obtained by in vitro transcription were cloned into pBSKneo U6′ and pGEM-T vectors, respectively. The cleavage activity of ribozymes against mouse caspase-7 was identified by cleavage experiments in vitro.RESULTS: Rz333 and Rz394 were designed and their DNA sequences were synthesized respectively. The expression vector of caspase-7 and plasmids containing Rz333 and Rz394 were reconstructed successfully. Ribozymes and caspase-7 mRNA were expressed by in vitro transcription.In vitro cleavage experiment showed that 243-nt and 744-nt segments were produced after caspase-7 mRNA was mixed with Rz333 in equivalent, and the cleavage efficiency was 67.98%. No cleaved segment was observed when caspase-7 mRNA was mixed with Rz394.CONCLUSION: Rz333 can site-specific cleave mouse caspase-7 mRNA, and it shows a potential for gene therapy of apoptosis-related diseases by down-regulating gene expression of caspase-7. | WeiZhang Qingxie Xia-QiuZhou ShanJiang You-XinJin | 2004 | World Journal of Gastroenterology2004，10，14： | 1 |
| 9 | E-Learning: the Future of English Language Learning显示文摘 | Yao Li Cao Ying & Wang Shanjiang | 2002 | Teaching Englishi In China2002，25，1： | 1 |
| 10 | Long noncoding RNAs:new insights in modulating mammalian spermatogenesis显示文摘Spermatogenesis is a complex differentiating developmental process in which undifferentiated spermatogonial germ cells differentiate into spermatocytes,spermatids,and finally,to mature spermatozoa.This multistage developmental process of spermatogenesis involves the expression of many male germ cell-specific long noncoding RNAs(lncRNAs)and highly regulated and specific gene expression.LncRNAs are a recently discovered large class of noncoding cellular transcripts that are still relatively unexplored.Only a few of them have postmeiotic;however,lncRNAs are involved in many cellular biological processes.The expression of lncRNAs is biologically relevant in the highly dynamic and complex program of spermatogenesis and has become a research focus in recent genome studies.This review considers the important roles and novel regulatory functions whereby lncRNAs modulate mammalian spermatogenesis. | Bahlibi Weldegebriall Sahlu Shanjiang Zhao Xiuge Wang Saqib Umer Huiying Zou Jinming Huang Huabin Zhu | 2020 | Journal of Animal Science and Biotechnology2020，11，2： | 1 |
| 11 | Sirtuin 3 regulation:a target to alleviateβ-hydroxybutyric acid-induced mitochondrial dysfunction in bovine granulosa cells显示文摘Background During the transition period,the insufficient dry matter intake and a sharply increased in energy consumption to produce large quantities of milk,high yielding cows would enter a negative energy balance(NEB)that causes an increase in ketone bodies(KBs)and decrease in reproduction efficiency.The excess concentrations of circulating KBs,represented byβ-hydroxybutyric acid(BHBA),could lead to oxidative damage,which potentially cause injury to follicular granulosa cells(fGCs)and delayed follicular development.Sirtuin 3(Sirt3)regulates mitochondria reactive oxygen species(mitoROS)homeostasis in a beneficial manner;however,the molecular mechanisms underlying its involvement in the BHBA-induced injury of fGCs is poorly understood.The aim of this study was to explore the protection effects and underlying mechanisms of Sirt3 against BHBA overload-induced damage of fGCs.Results Our findings demonstrated that 2.4 mmol/L of BHBA stress increased the levels of mitoROS in bovine fGCs.Further investigations identified the subsequent mitochondrial dysfunction,including an increased abnormal rate of mitochondrial architecture,mitochondrial permeability transition pore(MPTP)opening,reductions in mitochondrial membrane potential(MMP)and Ca^(2+)release;these dysfunctions then triggered the caspase cascade reaction of apoptosis in fGCs.Notably,the overexpression of Sirt3 prior to treatment enhanced mitochondrial autophagy by increasing the expression levels of Beclin-1,thus preventing BHBA-induced mitochondrial oxidative stress and mitochondrial dysfunction in fGCs.Furthermore,our data suggested that the AMPK-mTOR-Beclin-1 pathway may be involved in the protective mechanism of Sirt3 against cellular injury triggered by BHBA stimulation.Conclusions These findings indicate that Sirt3 protects fGCs from BHBA-triggered injury by enhancing autophagy,attenuating oxidative stress and mitochondrial damage.This study provides new strategies to mitigate the fGCs injury caused by excessive BHBA stress in dairy cows with ketosis. | Shanjiang Zhao Jianfei Gong Yi Wang Nuo Heng Huan Wang Zhihui Hu Haoyu Wang Haobo Zhang Huabin Zhu | 2023 | Journal of Animal Science and Biotechnology2023，14，4： | 1 |
| 12 | The traffic and behavioral effects of the I-35W Mississippi River bridge collapse显示文摘 | Zhu Shanjiang Levinson David Liu X Henry | 2010 | Transportation Research Part A: Policy and Practice2010，44，10： | 1 |
| 13 | Dynamic MR:A Dynamic Slot Allocation Optimization Framework for MapReduce Clusters显示文摘 | Tang Shanjiang Lee Bu-Sung He Bingsheng | 2014 | IEEE Transactions on Cloud Computing2014，2，3： | 1 |
| 14 | Improve meat production and virus resistance by simultaneously editing multiple genes in livestock using Cas12i^(Max)显示文摘The clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated gene(Cas) system is continually optimized to achieve the most efficient gene editing effect. The Cas12i^(Max), a Cas12i variant, exhibits powerful DNA editing activity and enriches the gene editing toolbox. However, the application of Cas12i^(Max)in large domestic animals has not yet been reported. To verify the efficiency and feasibility of multiple gene editing in large animals, we generated porcine fibroblasts with simultaneous knockouts of IGF2, ANPEP, CD163,and MSTN via Cas12i^(Max)in one step. Phenotypically stable pigs were created through somatic cell nuclear transfer technology. They exhibited improved growth performance and muscle quality. Furthermore, we simultaneously edited three genes in bovine fibroblasts. A knockout of MSTN and PRNP was created and the amino acid Q-G in CD18 was precisely substituted. Meanwhile, no off-target phenomenon was observed by sum-type analysis or off-target detection. These results verified the effectiveness of Cas12i^(Max)for gene editing in livestock animals and demonstrated the potential application of Cas12i^(Max)in the field of animal trait improvement for agricultural production. | Jilong Ren Tang Hai Yangcan Chen Ke Sun Zhiqiang Han Jing Wang Chongyang Li Qingwei Wang Leyun Wang Huabing Zhu Dawei Yu Wei Li Shanjiang Zhao | 2024 | Science China(Life Sciences)2024，67，3： | 0 |
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