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您的检索式：作者名="Satish Kumar Ainala"
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| 1 | A novel D(−)-lactic acid-inducible promoter regulated by the GntR-family protein D-LldR of Pseudomonas fluorescens显示文摘Lactic acid has two stereoisomers of D(−)-and L(+)-forms,both of which are important monomers of biodegradable plastic,poly-lactic acid.In this study,a novel D-lactate inducible system was identified in Pseudomonas fluorescens A506,partially characterized and tested as biosensor.The D-lactate catabolic operon(lldP-dld-II)was negatively regulated through the inversely transcribed D-lldR(encoding a GntR-type regulator),where the repression is relieved by addition of D-lactate.The derepression was specific to D-lactate and marginally affected by L-lactate.The D-LldR-responsive operator,showing dyad symmetry and separated by one base,was located between+11 and+27 from the transcription start site of the lldP-dld-II operon.By site-directed mutagenesis,a motif with a dyad symmetry(AATTGGTAtTACCAATT),present in the upstream region of lldP,was identified as essential for the binding of LldR.D-lactate biosensors were developed by connecting the upregulation by D-lactate to a green fluorescent readout.About^6.0-fold induction by 100mM D-lactate was observed compared to Llactate. | Kalpana Singh Satish Kumar Ainala Yeonhee Kim Sunghoon Park | 2019 | Synthetic and Systems Biotechnology2019，4，3： | 0 |
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